Previously we reported the result of topical immunotherapy of warts with I -chloro-2,4-dinitrobenzene(DNCB) solution using two different methods. In this study, we treated the warts with DNCB ointment and compared the therapeutic result with previous study. In addition, in order to evaluate the possible rnechanlarn of DNCB-induced wart regression by histologic rnethod, 5 regressing warts were biopsied and we observed the histopathologic findings. Thirty-six patients with various warts which were resistant to the conventional measures were treated by challeng application with 100mg% or 10mg% DNCB ointment directly on several randomly selected wart lesions after sensitization. with l?000mcg of DNCR in solution on normal skin of upper arm. In 5 pat:ients showing regression of warts, one wart lesion which was not challenged with DNCR was biopsied. The results are summarized as follows; 1. We treated the 36 patients having various warts with DNCB ointment and 25 patients (69.4%) were cured. Most(72%) of the eured warts began to regress within one month. 2. We cornpared the therapeutic result in this study with the results in theprevious study in which we used DNCB solution with two different methods, Comparing the results obtained by the R different methods, we found no statistically different cure rate, however the present method might be more convement because the ointment can be maintained longer without any change of the ingredient concentration and can be applied more easily than the solution. 3. On histologic examination, we observed moderate to severe patchy perivasce
Arm ; Dinitrochlorobenzene* ; Humans ; Immunotherapy* ; Skin ; Warts*

We presented two cases of bullous dermatoses in childhood. The first case was a 13-year-old boy who had numerous tense bullae on the scalp, both forearms and dorsa of hands, trunk and both thighs in symmetrical distribution, but he had not any oral lesion. On histologic examination, subepidermal bullae containing many neutrophils and only a few eosinophils were demonstrated. The lesions began to subside prornptly since 2 days after ornl administration of prednisolone and there was no evidence of recurrence during the follow-up studies of 4 months. The second case was a 14-year-old boy who had erythematous macules, vesicles, bullae and crusts scattered irregularly on almost entire body surface and had some oral lesions. Histologically erythematous macular lesions revealed many microabscesses composed almost entirely oi neutrophils on the tip of dermal papillae but vesicular lesions showed intraepidermal bulla containing numerous eosinophils exclusively. His skin lesions were well suppressed by DDS but reappeared one or two days after cessation of DDS. We thought these two cases were not the typical cases of bullous pemphigoid or dermatitis herpetiforrnis. We did not perform immunologic studies, so we cannot assure that these two cases were belong to either bullous pemphigoid or dermatitis herpetiformis and the so-called benign chronic bullous dermatosis of childhood.
Adolescent ; Dermatitis ; Dermatitis Herpetiformis ; Eosinophils ; Follow-Up Studies ; Forearm ; Hand ; Humans ; Male ; Neutrophils ; Pemphigoid, Bullous ; Prednisolone ; Recurrence ; Scalp ; Skin ; Skin Diseases ; Skin Diseases, Vesiculobullous* ; Thigh ; Transcutaneous Electric Nerve Stimulation

We presented a case of perifolliculitis capitis abscedens et suffodiens associated with acne conglobata in 40-year-old rnale. He had a sausage-shaped plaque containing many fistular tracts on right temnporal area and cicatrizing alopecia on occiput. On the middle portion of upper back he had a adult fist-sized plaque containing many fistular tracts. Histopathologically epidermis showed mild byperkeratosis and keratotic plugging and in the dermis most hair follicles were destroyed and replacel by fibrous tissue but there were moderxte cellular infiltration composed of neutrophils, lymphocytes a.nd hist.iocytes around the remained hair follicles. On serum electrophoresis alpha,-globulin was elevated and gamna globulin was the upper liviit of nornal variation. Skin tests with murnps vaccine, dinitrochloro benzene (DNCB) and old tuberculin disclosed the evidence of de reased c "ll-mediated immunity(CMI). All th se findings suggest thzt immune rriechanisni r,-ay be concerned ivith the pathogenesis in this case. He wa.s treated with th internal administration of antibiotic and corticosteroid and with surgical opening and curettage of fistular tracts
Acne Vulgaris ; Adult ; Alopecia ; Benzene ; Curettage ; Dermis ; Electrophoresis ; Epidermis ; Hair Follicle ; Humans ; Lymphocytes ; Neutrophils ; Skin Tests ; Tuberculin

BACKGROUND: Alpha hydroxy acids (AHAs) are known to diminish corneocyte cohesion at the innermost levels of the stratum corneum and have been used in the treatment of various disorders of keratinization. However, their effect on skin barrier function and their irritant potential is not fully understood. OBJECTIVE: Our study was done to evaluate the skin irritancy of AHAs in normal human skin. METHODS: Patches with 1%, 5% and 10% solutions of lactic acid (LA) and glycolic acid (GA) were applied to the volar forearm of 20 healthy volunteers for 24 hours using large Finn chambers with filter paper. Visual scores, erythema (E-) index and transepidermal water loss (TEWL) were measured at 30 min, 24 h and 48 h after removal of the patches. RESULTS: The results are summarized as follows. 1. Visual scores were 0.1+/-0.3 (1%), 0.5+/-0.6 (5%) and 1.1+/-0.8 (10%) at 24 h after removal of LA, and were 0.2+/-0.4 (1%), 0.6+/-0.6 (5%) and 1.0+/-0.7 (10%) at 24 h after removal of GA. They were increased in proportion to the concentrations and there were significant differences in skin responses between the control and each concentration of the solutions. 2. E-indices were 9.1+/-2.1 (control), 8.8+/-1.8 (1%), 9.0+/-2.6 (5%) and 10.5+/-3.9 (10%) at 24 h after removal of LA, and were 9.4+/-1.8 (control), 9.3+/-2.3 (1%), 10.0+/-3.0 (5%) and 11.1+/-3.5 (10%) at 24 h after removal of GA. They were not increased in the patch areas of 1% and 5% solutions in both the LA and GA group, but were significantly increased in the patch areas of 10% solutions in both the LA and GA group. 3. TEWL values were 7.3+/-2.3 (control), 8.3+/-4.0 (1%), 9.8+/-4.5 (5%) and 16.7+/-9.1 (10%) at 24 h after removal of LA, and were 8.1+/-3.2 (control), 7.8+/-3.8 (1%), 8.6+/-3.0 (5%) and 10.9+/-4.1 (10%) at 24 h after removal of GA. They were not increased in the patch areas of 1% LA, 1% GA and 5% LA, but there were high significant differences between the controls and 10% solutions of both LA and GA. CONCLUSION: Visual scores were increased in all concentrations of AHAs tested, but the increase in E-index and TEWL values were not significant or minimal in 1% and 5% solutions of AHAs. These findings suggest that AHAs could be classified as non-corrosive irritants.
Erythema ; Forearm ; Healthy Volunteers ; Humans* ; Hydroxy Acids* ; Irritants ; Lactic Acid ; Skin*

The object of this study was to determine the difference of radiation effect in different fractional does and to establish optimal fractionation schedule on the whole-abdominal X-ray irradiation. Total 160 mice were irradiated with 150 KVP, 15 mA orthovoltage x-ray machine and two different fractionation (100 cGy/Fr. and 200 cGy/Fr.) were used. Body weight, hemoglobin and WBC count with differential count were analyzed according to the same amount of total dose, same field size and two different fractionation schedules. The results of this study were summarized s follows: There was no significant difference in body weight and hemoglobin concentration by sex or fractional dose. Leukopenic change was prominent in the 3,000 cGy irradiation group and the proportion of decrease was remarkable in the 200 cGy/Fr. Group than 100 cGy/Fr. Group. Progressive decrease of lymphocyte count and reciprocal increase of neutrophil count were noted as dose increment. The effect of the fractional does on WBC count and proportion of lymphocyte were significant. This suggests that judicious selection of fractional dose may be important in clinical radiotherapeutic practice.
Animals ; Appointments and Schedules ; Body Weight* ; Lymphocyte Count ; Lymphocytes ; Mice* ; Neutrophils ; Radiation Effects*

Laureth 9 is a nonionic surfactant and it is used as a sclerosing agen; in the treatment of esophageal and gastric varices and a local anesthetics. We report a 20-year-old male patient presenting with features of alergic contact dermatitis due to laureth 9 used in the oxoralen, ointment.
Anesthetics, Local ; Dermatitis, Allergic Contact* ; Dermatitis, Contact ; Esophageal and Gastric Varices ; Humans ; Male ; Young Adult

Normal lymphocyte transfer (NLT) test is composed of two peaks of delaycd. hypersensitivity reaction of erythema and/or induration developed at the site of intradermal inoculatioa of allogenic lymphocyte. It has been suggested that NLT test is a px.siblc homograft raaction, how var tlv exact mecha.nism is remained to be resolved. In leprosy, there has been accumulating evidenc. indicating defective cell mediated. immunity detected by varioua immunological tests. The present study was undertaken to evaluate the immune capability of patients with leprosy using NLT test. A total of 20 hea,lthy volunteers, 20 lepromatous and 22 tuberculoid leprosy patients who were under antileprosy chemotherapy with DDS for varying periods, entered this study. Lymphocytes from hepatitis associati antigen (HAA) negative donor were isolated with Ficoll- Hypaque density gradient method and the cell concentration was adjusted by normal saline so as to give 2, 5 million cells per 0. 1 ml. Each recipient was given 0. 1 ml lymphocyte suspension on the flexor surface of th right forearm intrad.ermally using 1 ml tuberculi n syringe with a 26 gauge needle. The appearance and the size of induration was checked daily for 2 weeks. In this atudy, the NLT reaction pattem was classified as follows: a. normal pattem showing the first peak and second peak of reaction. b. first peak only showing the first peak, but no second peak, of reaction. c. flat pattem showing neitger the first peak nor the second peak of reaction The results were as follows: 1. There were normal pattem in sixteen persons(80%) and first peak only in 4 persons(20%) among twenty healthy human volunteers. 2. There were normal pattem in four patients(20%), first peak only in six patients(30%) and flat pattem in ten patients(50%) among twenty letromatous leprosy patients. 3. There were normal pattem in twelve patients(54.5%), first peak only in eight patients(36.4%) and flat pattem in two patients(9.1%) among twenty-two tuberouloid leprosy patients. These findings suggest that NLT test may be useful in evaluating the immune capability in leprosy and host factor in belived to be important in the development of both first and second peaks of NLT test.
Allografts ; Diatrizoate ; Drug Therapy ; Erythema ; Forearm ; Healthy Volunteers ; Hepatitis ; Humans ; Hypersensitivity ; Immunologic Tests ; Leprosy* ; Leprosy, Tuberculoid ; Lymphocytes* ; Needles ; Syringes ; Tissue Donors ; Volunteers

No abstract available.
Humans ; Rectus Abdominis* ; Tissue Donors*

Using a monoclonal antibody OKT 6, we nvestigated Langerhans cells by indirect immunoperoxidase technique in 60 skin specimens of fetuses from 3 months of gestation period to 10 months and 21 skin specimens of adults for the study of their development and the comparison of their density in fetus with adult. The results were as follows: 1. Langerhans cell population density of fetus was 52/mm in 4 months of gestation period, l07/mm in 5 months,136/mm in 6 months, l97/mm in 7 months, 235/mm in 8 months, and 311/mm in 10 months and there was a tendency to increase from 4 months to 1Q months. The earliest fetal skin specimen showing OKTg(+) Langerhans cell was that of 13 weeks and 2 days of gestation period. 3. Langerhans cell population density of back skin in fetus was 12.6% in 5 months, 16.0%, in 6 months, 20.8% in 7 months, 26.4% in 8 months and 36, 2% in 10 months of 900/mm in adult back skin. 4, Langerhans cell population density in adult was 432/mm in the skin of shin, 363/mm in prepuce, and 90Q/mm in back skin and there was statistically significant in difference between prepuce and back skin(p<0.00l).
Adult ; Fetus* ; Humans ; Immunoenzyme Techniques ; Langerhans Cells* ; Population Density ; Pregnancy ; Skin