Lesional and normal skins in 10 patients of fixed drug eruption were studied. Number and vertical distance of rete ridges were measured in H & E stained specimens. Amount of free melanin and number of melanocytes were counted in dopa stained epidermal sheets. According to the number of recurrence, activity and number of melanocytes see med to be changed. The lesions of a few recurrence showed only active melano cytes, however, those of frequent recurrenoe increased number of melanocytes with marked elongation of rete ridges. It is supposed that the numerical increase of melanocytes were from the elongation of rete redges.
Dihydroxyphenylalanine ; Drug Eruptions* ; Humans ; Melanins ; Melanocytes* ; Recurrence ; Skin

Suction is a useful tool for separation of epidermis from dermis in vivo. With appropriate suction pressures and durations applied, cellular damage of the separated epidermis can reduce, This study was performed to examine the appropriste suction pressures and durations in the lhsional and normal skin of four vitiligo patients who wanted to take epidermal autograft. The suction pressures and durations for making the blisters of similar morphology were different according to person. Microscopic changes in the blisters of similar morphology were not very different, and suction of 200 to 250 mmHg for 2 to 3 hours with a 2cm diametered suction cup was considered to be acceptable. Obvious damage to keratinocytes and diminish of melanacyte number were observed with much more powerful and longer suction such as suction of 350 to 400 mmHg for 3 to 4 hours. The level of dermo-epidermal separation was between basal cell membrane.
Autografts ; Blister ; Cell Membrane ; Dermis* ; Epidermis* ; Humans ; Keratinocytes ; Skin ; Suction* ; Vitiligo*

No abstract available.
Drug Eruptions*

HLA-A, B, C and HLA-DR are very important in the regulation of immune response and of transplant success or failure, but there have been a few reports about F3I A expression in melanocyt.es, a minor population of cpidermal cells. In the treatment of stable or segmental vitiligo, allogeneic melanocytes graft could t)e potentially useful if rejection does not. occur. The aim of this study was to examine the expression of HI.A-A, B, C and HI.A-DR in cultured hurnan melanocytes with and without. stimulation by INF-r and suction blister fliid. All strains of cultured me- lanocytes expressed HLA-A, B, C regardless of stmulation, but HLA-DR was expressed only following stimulation of INI-r. HLA expression in me]anocytes appears to be similar to that in keratinocytes.
Blister* ; HLA-A Antigens ; HLA-DR Antigens* ; Humans* ; Keratinocytes ; Melanocytes* ; Suction* ; Transplants ; Vitiligo

Sensitization to Cenasert®, a broad spectrum antimicrobial for the treatment of leukorrhea, is rare. Perineal contact dermatitis due to vaginal suppositories has never been reported in the medical literature. We report a 31-year-old woman, who developed perineal skin eruptions following the treatment of leukorrhea with Cenasert® vaginal suppositories. Patch tests showed positive reactions to Cenasert® suppositories and to the three active ingredients of Cenasert®. Vaginal suppositories can sensitize not only the vagina but also the surrounding perineum due to leakage of the allergen from the vaginal vault.
Adult ; Dermatitis, Allergic Contact* ; Dermatitis, Contact ; Female ; Humans ; Leukorrhea ; Patch Tests ; Perineum ; Skin ; Suppositories* ; Vagina

Two cases of pseudoxanthoma elasticum associated with angioid streaks in both eyes were presented and the literatures were reviewed. The affected sisters were 15 and 18 years female among the five siblings. They had pin head sized yellowish maculopapular eruptions on the sides of the neck and axillae, and also visual distubance of the both eyes. They seemed to be autosomal recessive inberitance in their family background. There were no other complications.
Angioid Streaks ; Axilla ; Female ; Head ; Humans ; Neck ; Pseudoxanthoma Elasticum* ; Siblings

Although there are many reports on the splenic (systemic) T cell response after Toxoplasma gondii infection, little information is available regarding the local T cell responses of peritoneal exudate cells (PEC) and gut intraepithelial lymphocytes (IEL) following peroral infection with bradyzoites. Mice were infected with 40 cysts of the 76K strain of T. gondii, and then sacrificed at days 0, 1, 4, 7 and 10 postinfection (PI). The cellular composition and T cell responses of PEC and IEL were analyzed. The total number of PEC and IEL per mouse increased after infection, but the ratio of increase was higher in IEL. Lymphocytes were the major component of both PEC and IEL. The relative percentages of PEC macrophages and neutrophils/eosinophils increased significantly at day 1 and 4 PI, whereas those of IEL did not change significantly. The percentage of PEC NK1.1 and gamma delta T cells peaked at day 4 PI (p < 0.0001), and CD4 and CD8 alpha T cells increased continuously after infection. The percentages of IEL CD8 alpha and gamma delta T cells decreased slightly at first, and then increased. CD4 and NK1.1 T cells of IEL did not change significantly after infection. IFN-gamma-producing PEC NK1.1 T cells increased significantly from day 1 PI, but the other T cell subsets produced IFN-gamma abundantly thereafter. The proportion of IEL IFN-gamma-producing CD8 alpha and gamma delta T cells increased significantly after infection, while IEL NK1.1 T cells had similar IFN-gamma production patterns. Taken together, CD4 T cells were the major phenotype and the important IFN-gamma-producing T cell subsets in PEC after oral infection with T. gondii, whereas CD8 alpha T cells had these roles in IEL. These results suggest that PEC and IEL comprise different cell differentials and T cell responses, and according to infection route these factors may contribute to the different cellular immune responses.
Acute Disease ; Animals ; Ascitic Fluid/cytology/*metabolism ; Female ; Interferon Type II/*biosynthesis ; Intestinal Mucosa/cytology ; Lymphocytes/*metabolism ; Mice ; Mice, Inbred C57BL ; Support, Non-U.S. Gov't ; T-Lymphocyte Subsets/*immunology ; Toxoplasmosis/*immunology

No abstract available.
Thoracic Wall* ; Thorax*

No abstract available.
Glomerular Filtration Rate* ; Plasma*

Consecutive twelve patients' twelve eyes with complicated pars planitis underwent pars plana vitrectomy at our center in the period from January 1988 to December 1990. Patients' ages ranged from 24 to 60(mean 39.9)years and all were male. Nine patients had histories of topical or systemic steroid therapy and at the time of surgery, all eyes were quiet from active inflammation. With various degrees of vitreal opacities, five eyes had macular pucker or membrane only and in other seven eyes, retinal detachments of either tractional or tractional-rhegmatogenous origin were arisen. In the later group, macular hole was associated in two eyes. Surgical procedures combined were membrane peeling(five eyes), scleral buckling(ten eyes), fluid/air-gas exchange(eight eyes), lensectomy(two eyes) and photocoagulation(two eyes). During the follow-up period ranged from four to twenty-one(mean 7.75)months after first operations, the uveitis recurred or continued to be activated in four eyes and as a complication, retinal detachment occurred in two eyes which had macular pucker only preoperatively. One refused reoperation and the other was reattached successfully by second operation. Final visual acuities were improved in eight eyes, stationary in one and worsen in three. The causes of worsen vision were retinal detachment, macular edema and macular slanting by buckle respectively. Preoperatively, the majority of eyes(10 eyes: 84%) had less than 0.09 vision and postoperative vision turned over better than 0.09 in 7 eyes(59%). Although we did not confirm extinguished therapeutic effects of vitrectomy on the pars planitis perse, our results revealed some positve role of surgical intervention in the complicated pars planitis.