1.Clinicopathologic Analysis of the Liver Explant with Severe Hepatitis A Virus Infection.
Joo Young KIM ; Sung Gyu LEE ; Shin HWANG ; Ji Hoon KIM ; Se Jin JANG ; Eunsil YU
Korean Journal of Pathology 2011;45(Suppl 1):S48-S52
The incidence of severe hepatitis A virus (HAV) infection has been increasing. However, clinicopathologic features of severe HAV infection that lead to liver transplantation (LT) have not been reported in Korea. We retrieved 16 LT cases with HAV infection during the last 3 years at Asan Medical Center, Seoul, Korea. Fifteen cases progressed to hepatic encephalopathy. Thirteen cases survived with or without complications, and three patients died of sepsis. The explanted liver showed massive or zonal necrosis with moderate to severe cholestasis. The zonal distribution of necrosis was frequently associated with endothelialitis of portal and/or central veins. Degenerative changes of hepatocytes were various in degree and distribution. Viral inclusions were suspected in two cases. Although HAV infection is usually confirmed by serological tests, significant venulitis of central and/or portal veins and viral inclusions, which are rarely observed, can suggest an HAV infection as a cause of massive hepatic necrosis of unknown mechanism.
Cholestasis
;
Fluconazole
;
Hepatic Encephalopathy
;
Hepatitis
;
Hepatitis A
;
Hepatitis A virus
;
Hepatocytes
;
Humans
;
Incidence
;
Korea
;
Liver
;
Liver Transplantation
;
Massive Hepatic Necrosis
;
Necrosis
;
Portal Vein
;
Sepsis
;
Serologic Tests
;
Veins
2.Permanent Preserving the Bony Fragments Digged Up from Haemi Nameless Martyrdom Holy Place Burial Sites.
Won Sik KIM ; O Yu KWON ; Chang Yong LEE ; Woong Ghi SHIN ; Soo Il KIM
Korean Journal of Physical Anthropology 1996;9(2):135-139
Bone materials diggd up from the Haemi nameless martyrdom holy place burial sites were severely broken into fragments of various size, clay matrix filled the bone marrow cavities, grass roots were spreaded into the periosteum and the fragments were very soft enough to be flaken. To make permanent specimens, bony fragments impregnated within unsaturated polyester resin with catalyzer MEKP, promotor 8% cobalt octoate, and UV absorber. Permanent specimens were so very transparent that all the macroscopic structures can be observed, and they were hard enough not to be broken or cracked. This method of preserving bony fragments is considered to be used in archaeologic preservation and in making teaching materials of various human organs or tissues.
Bone Marrow
;
Burial*
;
Cobalt
;
Humans
;
Methods
;
Periosteum
;
Poaceae
;
Polyesters
;
Teaching Materials
3.A study of relationship between hypertension and hyperlipidemia in the health screening center.
Yong Chul KIM ; Im Yu KIM ; Youn Sug CHOI ; H cheol SHIN ; Eun Sook PARK
Journal of the Korean Academy of Family Medicine 1992;13(5):410-419
No abstract available.
Hyperlipidemias*
;
Hypertension*
;
Mass Screening*
4.A Case of CHAP Syndrome.
Man Wook SEO ; Ji Sung KIM ; Seong Yong YU ; Byeung Soo SHIN ; Young Hyun KIM
Journal of the Korean Geriatrics Society 2001;5(4):348-353
CHAP syndorme consists of choreoathetosis, orofacial dyskinesia, affective change, hypotonia, and pseudobulbar signs. CHAP syndrome was firstly des- cribed with onset 1 to 7 days after induced profound hypothermia and complete circulatory arrest for con- genital heart lesion repair. Since then, only a few cases have been reported until now. Case We report a patient who demonstrated all of the elements of the CHAP syndrome in severe cachexic state. Brain SPECT(99mTC-ECD) shows decreased perfusion in left fronto-parieto-temporal lobe. Comments The pathogenesis of CHAP syndrome is obscure. However our case led us to suggest that more essential component pre- disposing to the CHAP syndrome is hypoxia rather than hypothermia. Furthermore, it seems to be related with circulatory insufficiency in extrapyramidal nervous systems.
Anoxia
;
Brain
;
Heart
;
Humans
;
Hypothermia
;
Movement Disorders
;
Muscle Hypotonia
;
Nervous System
;
Perfusion
5.Development of Test System for Detection of Antibody to Human Immunodeficiency Virus Type 1 Subtype O.
Young Shik CHO ; Gun Woo HA ; Sunyoung KIM ; Seung Shin YU ; Sang Gook LEE ; Myung Hwan CHO ; Hyung Sik SHIN
Journal of the Korean Society of Virology 1998;28(1):31-38
In Korea, all domestic made test systems for detecting antibodies in HIV-1 contain the antigens from human immunodeficiency type 1 (HIV-1) subtype B. However, because HIV-1 subtype O is significantly different in amino acid sequences from all other subtypes of HIV-1, there has been a need for developing a test for detecting antibodies in subtype O. For this purpose, the entire nucleotide sequence corresponding to the extracellular domain of the transmembrane glycoprotein of HIV-1 subtype O was synthesized with consideration of Escherichia coli cordon usage. Various regions of the extracellular domain were cloned into E. coli expression vectors and tested for levels of protein production. The nucleotide sequence, named ECTM, that can encode a 129 amino acid-long peptide, was found to be expressed at a high level in E. coli. The protein of approximately 17 kDa specifically reacted with sera from individuals infected with HIV-1 subtype O. The ECTM protein was purified to near homogeneity by the CM-T gel chromatography, using concentrated, denatured inclusion bodies. In Western blot analysis, the purified viral antigen reacted with sera from individuals infected with subtype O more efficiently than subtype B. The enzyme linked immunoabsorbent assay (ELISA) system was developed using the subtype O viral protein and compared with the commercially available kit lacking the antigens from subtype O. The ELISA kit containing the subtype O antigen ECTM alone efficiently reacted with sera from individuals infected with subtype O. The subtype O antigen-containing kit produced a positive absorbence even when sera were diluted 512-fold, suggesting a high sensitivity. The commercially available kit also reacted with subtype O sera, but produced a negative result at a dilution of 8-fold. Our results suggest that the currently available kit may not be able to efficiently detect subtype O sera and that the viral protein developed in this study may be added to the current system to maximize the detection of sera from individuals infected with subtype O.
Amino Acid Sequence
;
Antibodies
;
Base Sequence
;
Blotting, Western
;
Chromatography, Gel
;
Clone Cells
;
Enzyme-Linked Immunosorbent Assay
;
Escherichia coli
;
Glycoproteins
;
HIV*
;
HIV-1*
;
Humans*
;
Inclusion Bodies
;
Korea
;
O Antigens
6.The Effect of Senior Simulation on Nurses' Attitude Toward the Elderly.
Su Jeong YU ; Shin Mi KIM ; Yun Jung LEE
Journal of Korean Academy of Nursing 2004;34(6):974-982
PURPOSE: This study was performed to explore the effect of senior simulation on nurses' attitudes toward the elderly. METHOD: Twenty-seven nurses working in various settings such as acute hospitals, community health centers, geriatric hospitals, and clinics were recruited. Among them, 25 subjects completed the whole experimental protocol. Aging Semantic Differential Scaling was utilized to evaluate attitudes toward the elderly and 'Suit for Experiencing Being Aged' from the Sakamoto Model was provided for the experiment. Before and after the experiment subjects filled out questionnaires. RESULT: Attitude score before experiment was 4.36, which indicates neutral attitude. Objective attitude scores were not different significantly after experiment. However subjective statements indicated attitude changes in a positive way. CONCLUSION: Senior simulation can affect nurses' attitude toward elderly in subjective way. That is, nurses became more empathetic and understanding to elderly's physical limitations and felt more initiative nursing approach were needed in caring elderly.
Adult
;
*Aged
;
*Attitude of Health Personnel
;
Female
;
Humans
;
Nurse-Patient Relations
;
Nurses/*psychology
;
*Patient Simulation
7.Relationship of Insulin like Growth Factor I with Pharmacologically Stimulated Growth Hormone Secretion in Growth Hormone Deficient Children.
Phil Soo OH ; Ki Joong KIM ; Young Iee YU ; Jeh Hoon SHIN
Journal of the Korean Pediatric Society 1995;38(10):1394-1403
No abstract available.
Child*
;
Growth Hormone*
;
Humans
;
Insulin*
;
Insulin-Like Growth Factor I*
8.Histopathological Causes of Late Liver Allograft Dysfunction: Analysis at a Single Institution.
Eun SHIN ; Ji Hoon KIM ; Eunsil YU
Korean Journal of Pathology 2013;47(1):21-27
BACKGROUND: We summarize our experience in the pathological diagnosis of late complications of liver transplantation (LT) to better understand the causes of late allograft dysfunction in a population mostly composed of patients with hepatitis B virus (HBV) infection. METHODS: We reviewed 361 post-transplant liver biopsies from 174 patients who underwent LT and first presented with liver function abnormalities 3 months post-procedure. The underlying diseases included HBV-associated liver disease (77%), toxic or alcoholic liver disease (10.3%), hepatitis C virus (HCV)-associated liver disease (8.6%), primary biliary cirrhosis (1.2%), primary sclerosing cholangitis (1.2%), and metabolic disease (1.7%). RESULTS: The three most common late complications were acute rejection (32.5%), recurrent disease (19.1%), and biliary complication (17.1%). Patients who underwent LT for HBV infection or for drug- or alcohol-related liver disease had a lower incidence of recurring disease than those who underwent transplantation for HCV infection. During post-transplantation months 3-12, acute rejection was the most common cause of allograft dysfunction and recurring disease was the leading cause for allograft dysfunction (p=0.039). The two primary causes of late allograft dysfunction have overlapping histological features, although acute rejection more frequently showed bile duct damage and vascular endothelialitis than recurring HBV infection, and recurring HBV infection had more frequent lobular activity and piecemeal necrosis. CONCLUSIONS: The causes of late liver allograft dysfunction are closely associated with the original liver diseases and the period after LT. Careful attention is required for differential diagnosis between acute rejection and recurrent HBV.
Bile Ducts
;
Biopsy
;
Cholangitis, Sclerosing
;
Diagnosis, Differential
;
Hepacivirus
;
Hepatitis B virus
;
Humans
;
Incidence
;
Liver
;
Liver Cirrhosis, Biliary
;
Liver Diseases
;
Liver Diseases, Alcoholic
;
Liver Transplantation
;
Metabolic Diseases
;
Rejection (Psychology)
;
Transplantation, Homologous
;
Transplants
9.Control Mechanisms of Ovarian Follicle Development by Follicle Stimulating Hormone and Pituitary Adenylate Cyclase-activating Polypeptide.
Yu Il LEE ; Jin Ok SHIN ; Mi Young KIM ; Sang Young CHUN
Korean Journal of Fertility and Sterility 2006;33(1):15-24
OBJECTIVE: Pituitary adenylate cyclase-activating polypeptide (PACAP), a novel hypothalamic neuropeptide, has been suggested to play a role in ovarian folliculogenesis. The present study evaluated the effect of PACAP on the growth of preantral follicles. METHODS: Preantral follicles were mechanically isolated from ovaries of 21-day-old rats and cultured in groups for 3 days in serum-free medium in the absence or presence of PACAP-38 (10-6 M). RESULTS: Treatment with PACAP-38 resulted in an increase in follicle diameter by 75% whereas treatment with follicle stimulating hormone (FSH) increased follicle diameter by 65%. PACAP-38 treatment enhanced the granulosa cell proliferation as measured by thymidine incorporation analysis. Furthermore, the production of progesterone by cultured granulosa cells and GFSHR-17 cell line was stimulated by PACAP-38. Interestingly, PACAP enhanced FSH action on stimulation of SF-1 and aromatase gene expression. CONCLUSION: The present results demonstrate that PACAP stimulated preantral follicle growth by potentiating proliferation and by stimulating steroidogenesis.
Animals
;
Aromatase
;
Cell Line
;
Female
;
Follicle Stimulating Hormone*
;
Gene Expression
;
Granulosa Cells
;
Neuropeptides
;
Ovarian Follicle*
;
Ovary
;
Pituitary Adenylate Cyclase-Activating Polypeptide*
;
Progesterone
;
Rats
;
Thymidine
10.The Effect of Knowledge and Altruism on the Blood Donation Behavior among High School Students.
Korean Journal of Blood Transfusion 2010;21(1):16-24
BACKGROUND: This study was designed to evaluate the effect of knowledge and altruism on the blood donation behavior among high school students. METHODS: This cohort study enrolled 299 high school students who had not previously donated blood, but who had participated in a school group blood donation program at Gwangju.Chonnam Red Cross Blood Center. An initial survey was conducted 1 month before the planned blood donation program. The blood donation program was conducted from May to June 2009. A follow-up survey was conducted 1 month later. The first questionnaire consisted of 42 questions on general characteristics, knowledge of blood donation, altruism and public relations issues associated with blood donation. The second questionnaire consisted of 6 questions about the motivation for blood donation and the reasons for not donating. RESULTS: Among the enrolled students, there were 143 (47.8%) donors and 156 (52.2%) students completed and returned the questionnaires. The level of knowledge was not significantly associated with blood donation (P=0.959). The more altruistic group had an increased blood donation rate (OR=2.08; 95% confidence interval [CI] 1.29~3.66) compared with the less altruistic group. After adjusting for school, religion, the parents' income, parents' education and residence, the more altruistic group had a persistently increased blood donation rate (OR=1.98; 95% CI 1.18~3.32). CONCLUSION: In this study, altruism was associated with blood donation. Therefore, we need to develop an education program to enhance altruism in order to increase blood donation from high school students.
Altruism
;
Blood Donors
;
Cohort Studies
;
Follow-Up Studies
;
Humans
;
Motivation
;
Public Relations
;
Red Cross
;
Tissue Donors
;
Surveys and Questionnaires