1.Development of a LC-MS/MS for Quantification of Venlafaxine in Human Plasma and Application to Bioequivalence Study in healthy Korean Subjects.
Hyun Ku KANG ; Min A KANG ; Hyun Jin KIM ; Yoo Sin PARK ; Shin Hee KIM ; Ju Seop KANG
Translational and Clinical Pharmacology 2014;22(1):35-42
A simple, rapid and selective liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is developed and validated for quantification of venlafaxine in human plasma with simple liquid-liquid extraction step consisted of extraction with ether and dichloromethane for 10 min and mixing with 1 M sodium acetate in human plasma using fluoxetine as an internal standard (IS). The analyte are separated using an isocratic mobile phase consisted of acetonitrile and 5 mM ammonium formate (4/3, v/v) on a isocratic YMC hydrosphere C18 (2.0x50.0 mm, 3.0 microm) column and analyzed by MS/MS in the multiple reaction monitoring (MRM) mode using the transitions of respective [M+H](+) ions, m/z 278.2-->260.3 and m/z 310.1-->148.1 for quantification of venlafaxine and IS, respectively. The standard calibration curves showed good linearity within the range of 1.0-200.0 ng/mL (r2=0.9986, 1/chi2 weighting). The lower limit of quantification (LLOQ) was 1.0 ng/mL. The retention times of venlafaxine and IS were 0.6 min and 0.7 min that means the potential for the high-throughput potential of the proposed method. In addition, no significant metabolic compounds were found to interfere with the analysis. Acceptable precision and accuracy were obtained for the concentrations over the standard curve range. The validated method was successfully applied to bioequivalence study after 75-mg of venlafaxine sustained-release (SR) capsule in 24 healthy Korean subjects.
Ammonium Compounds
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Calibration
;
Chromatography, Liquid
;
Ether
;
Fluoxetine
;
Humans
;
Ions
;
Liquid-Liquid Extraction
;
Methylene Chloride
;
Pharmacokinetics
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Plasma*
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Sodium Acetate
;
Tandem Mass Spectrometry
;
Therapeutic Equivalency*
;
Venlafaxine Hydrochloride
2.An investigation of the use of a general health examination center.
Eun Soo KU ; Hae Youn KIM ; Young Sung SUH ; Dong Hak SHIN ; Hi Young CHO ; Moon Ku KANG ; Hyo Geon BAE
Journal of the Korean Academy of Family Medicine 1991;12(7):52-62
No abstract available.
3.An investigation of the use of a general health examination center.
Eun Soo KU ; Hae Youn KIM ; Young Sung SUH ; Dong Hak SHIN ; Hi Young CHO ; Moon Ku KANG ; Hyo Geon BAE
Journal of the Korean Academy of Family Medicine 1991;12(7):52-62
No abstract available.
4.A Case of Myotonic Dystrophy with Prolonged Atrial Flutter.
Won Kwon KANG ; Dae Hoi KU ; Seung Hun SHIN ; Yeon Chae JEONG ; Eun Seok JEON ; Jong Hoon PARK
Korean Circulation Journal 1989;19(4):770-775
Myotonic dystrophy is a multisystemic disorder inherited as an autosomal dominant trait. The characteristic clinical features include the presence of myotonia, atrophy of the muscles of the face and the sternocleidomastoids and numerous nonmusclar manifestations such as cataracts, frontal baldness, gonadal dysfunctions and cardiac abnormalities. We experienced one case of myotonic dystrophy with prolonged atrial flutter in 30-year-old male who was admitted because of palpitation. We present this case with reviewing literatures.
Adult
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Alopecia
;
Atrial Flutter*
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Atrophy
;
Cataract
;
Gonads
;
Humans
;
Male
;
Muscles
;
Myotonia
;
Myotonic Dystrophy*
5.Effect of Smoking on Adult Periodontitis after Non-surgical Periodontal Therapy.
Young Chae PARK ; Jung Ku KANG ; Hyung Keun YOU ; Hyung Shik SHIN
The Journal of the Korean Academy of Periodontology 1997;27(2):305-315
The purpose of this study was to investigate the effects of smoking on adult periodontitis after non-surgical periodontal therapy. The study population consisted of 40 patients with moderate to advanced periodontitis. Smokers(n=20) were defined as individuals smoking at least twenty cigarettes per day at the time of the initial examination. The non-smoking group(n=20) consisted of individuals who were not smoking at the initial examination. The average age was 42.4 years for the smoking and non-smoking group. Examination regarding plaque index, gingival index, pocket depth and contrast phase microscope were performed. Evaluation were made at the first, the second and the fourth weeks after periodontal non-surgical therapy. The results were as follows: 1. Clinical indices including plaque index, gingival index, and pocket depth were decreased in both smoking and non-smoking group at the first, the second, and the fourth weeks. Especially, clinical indices of non-smokers were more significantly decreased than those of smokers. 2. Non-motile rods were increased and motile rods were reduced at the fourth week. spirochetes were reduced significantly in the non-smoking group at the fourth week. These results suggest that smoking play a minor role in adult periodontitis after non-surgical periodontal therapy.
Adult*
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Chronic Periodontitis*
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Humans
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Periodontal Index
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Periodontitis
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Smoke*
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Smoking*
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Spirochaetales
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Tobacco Products
6.A Case of Endobronchial Granular Cell Tumor Associated with Malignant Mediastinal Tumor.
Hyung Seok CHOI ; Ku Sub YUN ; Shin Eun CHOI
Tuberculosis and Respiratory Diseases 2000;48(1):96-102
Granular Cell Tumors(GCT) were originally described as myoblastic myomas. Subsequent scientific investigations elucidated the origin of this tumor as Schwann cells . Usually they have a predilection for head and neck, but also can occur in many other organs. This tumor is believed to originate from Schwann cells based on subsequent scientific investigations. Although it usually appears in the head and neck, it can also appear in other organs, as well. Endobronchial granular cell tumors are rather rare and should be differentiated from other common diseases of endobronchial location endobronchial diseases such as bronchogenic carcinoma and endobronchial tuberculosis, especially in Korea . We report a A case of a patient with an extremely rare condition of endobronchial granular cell tumor concurrent with malignant mediastinal tumor in a patient (delete) is reported.
Carcinoma, Bronchogenic
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Granular Cell Tumor*
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Head
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Humans
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Korea
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Myoblasts
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Myoma
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Neck
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Schwann Cells
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Tuberculosis
7.Effects of some herbal drugs on gingival fibroblast and periodontal ligament cellular activity.
Jin Soo DOO ; Jung Ku KANG ; Hyung Keun YOU ; Hyung Shik SHIN
The Journal of the Korean Academy of Periodontology 1997;27(3):459-468
Healing of periodontal tissues require the migration and proliferation of gingival fibroblasts and periodontal ligament cells. There is many evidences that the some agents like cytokines and polypeptide growth factors are mediate these cellular events in wound healing. Recently someone is interested in herbal drugs on periodontal tissue healing processes. The purpose of this study was to examine the effects of 4 herbal drugs, Carthami Flis, Moutan Radicis Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. on human gingival fibroblasts and periodontal ligament cells. Periodontal ligament cells and gingival fibroblasts were primarily cultured from extracted premolar with non-periodontal diseases. The powder from extracted herbal drugs were prepared with distilled water. Cells were cultured with DMEM at 37degrees C, 5% CO2, 100% humidity incubator, and treated with each herbal drugs with proper concentration for 1, 2, and 3 days. The cell activity was determined by ELISA reader using MTT assay. There was the most significant elevation in 10(-3)g/ml of almost herbal drugs on cellular activities. The result of this study demonstrated that Carthami Flis, Moutan Radicis Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. appears to have beneficial effect on healing process after periodontal treatment.
Bicuspid
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Carthamus tinctorius
;
Cytokines
;
Enzyme-Linked Immunosorbent Assay
;
Fibroblasts*
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Humans
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Humidity
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Incubators
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Intercellular Signaling Peptides and Proteins
;
Periodontal Ligament*
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Water
;
Wound Healing
8.A Clinical Study of the Effects of Time - interval and Temperature Change on the Arterial Blood Gases and PH .
Seung Hwan LEE ; Keon Sik KIM ; Hwa Ja KANG ; Dong Soo KIM ; Kwang Il SHIN ; Min Ku LYO
Korean Journal of Anesthesiology 1989;22(6):879-885
Blood gas samples are highly susceptible to preanalytic error due to improper methods of obtaining or handling the sample prior to delivery to the laboratory. The errors in the measurement of blood gas analysis are currently derived from the exposure of sample to atmosphere, effects of anticoagulant itself, temperature difference between the measuring electrode and drawn blood and the delay in running the sample. To study the effects of the delay in measuring the sample and the temperature difference between the measuring electrode and drawn blood on values of blood gases and pH, we analyzed the arterial sampling from the 24 patients who were taking elective surgery or on his/her recovery period with indwelling arterial catheter. The plastic sampling syringes were kept at 4 degrees C (refrigerator) or 22-24 degrees C (room temperature) and analyzed at regular intervals (1, 10, 30, 60,120 min) for 120 minutes. The following results were obtained: 1) When the arterial blood drawn from the anesthetized patients were stored at 4 degrees C, partial pressure of oxygen (PaO2) decreased significantly after 20 min, whereas those stored at room temperature decreased significantly after 10 min. 2) When the arterial blood drawn from the recovery patients were stored at 4 degrees C, PaO2 did not decrease significantly through the experimental period of 120 min. Although those stored at room temperature did not decrease significantly through the period of 120 min. 3) Partial pressure of carbon dioxide in the arterial blood (PaCO2,) drawn from the anesthetized patients increased significantly by 120 min. at 4 degrees C, whereas those at room temperature increased significantly after 20 min. 4) PaCO2, of the recovery patients increased signigicantly by 120 min. at 4 degrees C, whereas those at room temperature increased significantly after 30 min. 5) pH of the arterial blood drawn from either anesthetized or recovery patients decreased significantly by 120 min. at 4 degrees C, whereas those at room temperature decreased significantly after 60 min. 6) No significant changes of arterial oxygen saturation (SaO2) and content (CaO2) were noted in either anesthetized or recovery. patients in accordance with time elapsed at 4 degrees C or room temperature. In summary, as the changes of PO2 in particular higher than physiologic PO2 and PCO2 in the arterial blood stored at room temperature are significant in accordance with the delay in measuring, it would be advisable to analyze the sample in a short period of time or to store it in a cool place when the measuring will be delayed.
Atmosphere
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Blood Gas Analysis
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Carbon Dioxide
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Catheters
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Electrodes
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Gases*
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Humans
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Hydrogen-Ion Concentration*
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Oxygen
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Partial Pressure
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Plastics
;
Running
;
Syringes
9.Mechanism of Tissue Injury in Experimental Retinal Artery Occlusion: Morphological Study I.
Seung Jun PARK ; Shin Goo KANG ; Young In CHOI
Journal of the Korean Ophthalmological Society 2000;41(11):2450-2457
We used an animal model of laser-induced retinal artery occlusion to study the temporal and spatial patterns of tissue injury and to identify apoptosis-like morphologic changes.We performed fundus exam and fluorescein angiography for 2 weeks.Rats were sacrificed at 6 hours, 12 hours, 1 day, 2 days, 3 days, 7 days, and 14 days after arterial occlusion.The arterial reperfusion was seen from 6 hours to 4 days.We could observe vascular tortuosity and retinal hemorrhage in the fundus.Six hours after retinal arterial occlusion, there were a lot of cells which had chromatin condensation.One to three days membrane budding and apoptotic body were identifed after occlusion.Most of inner retinal layers were destroyed following 7days.These data demonstrated that retinal artery occlusion induces apoptotic cell death in the retinal ganglion cell and inner nuclear layer.
Apoptosis
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Cell Death
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Chromatin
;
Fluorescein Angiography
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Membranes
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Models, Animal
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Reperfusion
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Retinal Artery Occlusion*
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Retinal Artery*
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Retinal Ganglion Cells
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Retinal Hemorrhage
;
Retinaldehyde*
10.Synchronous Multiple Colorectal Cancer Occurring in Polyposis.
Young Chan HAM ; Il Myung KIM ; Jin YUN ; Sang Soo PARK ; Dong Gue SHIN ; Seong Ku KANG
Journal of the Korean Society of Coloproctology 2010;26(1):80-84
The frequencies of multiple synchronous carcinomas of the colon and the rectum have been reported to range from 2.1 to 6.3%. Currently, the frequency is higher in colorectal cancer patients, and the diagnosis is better due to the many diagnostic tools that have been developed. There are a few reported cases of five cancers in a patient at the same time. We report here on the case of five synchronous cancers arising from the colon and the rectum in a patient without a familial history of colon cancer or of genetic predisposing factor. The patient was a 62-yr-old woman who presented with frequently loose stool for six months and intermittent abdominal pain for two months. Colonoscopic examination revealed two adenocarcinomas, one each at the sigmoid colon and the rectum; the cancer in the sigmoid colon was obstructed at nearly 40 cm above the anal verge. Computed tomographic colonoscopy revealed many other polyps and masses in the colon and a metastatic mass at segment 8 in the liver. A total proctocolectomy and ileostomy were performed. Histologic evaluation revealed the five lesions to be adenocarcinomas invading the pericolic fat; 1 out of 30 lymph nodes was invaded by the cancer cells.
Abdominal Pain
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Adenocarcinoma
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Colon
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Colon, Sigmoid
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Colonic Neoplasms
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Colonoscopy
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Colorectal Neoplasms
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Female
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Humans
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Ileostomy
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Liver
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Lymph Nodes
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Polyps
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Rectum