1.THE EXPRESSION OF LECTIN RECEPTORS IN ADENOCARCIN-OMAS OF THE GASTROINTESTINAL TRACT
Academic Journal of Second Military Medical University 1985;0(06):-
Ten cases of gastrointestinal adenocarcinomas were histochemically investigated for their lectin receptors by avidin-biotin-peroxidase complex (ABC) method. Results showed that gastric adenocarcinomas expressed more types of lectin receptors than large bowel adenocarcinomas. Positive rates of G1c/Man-and GlcNAc-specific lectin receptors in those tumors were higher than those of Gal/GalNAc- and Fuc-specific lectin receptoes. From the stomach to rectum, the spectrum of the lectin receptors in pericarcinomatous tissues gradually changed from the gastric type to rectal type. The expression of lectin receptors in gastrointestinal adenocarcinomas was obviously different from that in their corresponding pericarcinomatous tissues. The increase and decrease of lectin receptor expression, the appearance and disappearance of some types of lectin receptors as well as the change of the receptor distribution are indirectly regulated by cellular genome.
2.THE RECEPTOR ROLE OF GLYCOPHORIN A DURING THE INVASION OF ERYTHROCYTES BY PLASMODIUM FALCIPARUM
Academic Journal of Second Military Medical University 1982;0(02):-
Glycophorin A(GPA)of human erythrocyte membrane is closely related with the invasion of erythrocytes by Plasmodium falciparum. Under the condition of the in vitro culture, the effects of GPA, anti-GPA-IgG, ?1-acid glycoprotein, wheat germ agglutinin, etc. on the invasion of erythrocytes by Plasmodium falciparum were observed in the research. The results showed that the combination of GPA with Plasmodium falciparum merozoites has high specificity, high affinity and a tendency to saturation, and produces specific biological effects. These confirm for the first time from the characteristics of the ligand-receptor interaction that GPA is a recptor recognized and bound by Plasmodium falciparum merozoites.
3.RAPID PURIFICATION OF WHEAT GERM AGGLUTINSN FROM WHEAT GERM
Academic Journal of Second Military Medical University 1981;0(03):-
Wheat germ agglutinin(WGA)with a higher activity of hemagglutination was purified from wheat germ by a modified method of ovomucoid-Sepharose 4B affinity chromatography.The average yield of purified WGA from 100g wheat germ acetone powder was 58.74?6.56mg. WGA showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent molecular weight of 34400.The specific haptens of WGA- were N-acetylglucosamine and sialic acid.
4.USE OF ABC METHOD IN THE STUDY OF LECTIN RECEPTORS IN LIVER TUMORS
Academic Journal of Second Military Medical University 1982;0(01):-
The high affinity interaction of avidin-biotin in immunoenzyrnatic techniques has provided a simple and sensitive method to localize antigens in formalin-fixed and paraffin-embedded tissues.The distribution of lectin receptors in primary hepatocellular carcinoma, int-ahepatic cholangiccarcinorria an.i cavernous hemangi-oma was studied for the first time by avidin-biotin-peroxidase complex (ABC) method.The results show that the method was simple, highly sensitive, and had gcod reproducibility and specificity as well as higher value of practical use.
5.Localization of WGA Receptors in Liver Tumor Tissues
Academic Journal of Second Military Medical University 1982;0(02):-
Receptors of wheat germ agglutinin (WGA) in liver tumors, such as hepatocellular carcinoma (HCC), intrahepatic cholangiocellular carcinoma(ICC) and so on, were histologically localized by avidin-biotin-peroxidase complex (ABC) method. Normal, cirrhostic and pericarcinomatous liver tissues were used as controls. The result showed that WGA receptors in HCC were irregularly distributed, which was significantly different to the band-like distribution of WGA receptors in the controls and to the distribution in ICC. After the treatment with neuraminidase, the distributions of WGA receptors changed apparently. It is suggested that the characteristics of WGA receptor distributions in cells could be helpful to the diagnosis and differential diagnosis of liver tumors.
6.Mismatch between the short celphalomeduallary nail and the anterior femoral bow in Chinese intertrochanteric fractures: a postoperative radiological study
Chinese Journal of Orthopaedic Trauma 2012;14(2):103-107
Objective To observe the matching between the straight short femoral cephalomedullary nail (PFNA-Ⅱ) and the femoral anterior bow in Chinese patients with intertrochanteric fracture. Methods A retrospective study was performed to analyze the postoperative lateral X-rays of 158 patients with intertrochanteric fracture who had been treated by PFNA- Ⅱ between August 2009 and December 2010.They were 35 men and 123 women,with a mean age of 77.2 years.The relationships between the distal tip of the medullary nail and the anterior cortex of the femur were observed according to a self-designed 6-grade scale (grade 0:central location of the nail tip in the medullary canal; grade 1:deviated location without contact with the cortex; grade 2:contact< 1/3 cortex thickness; grade 3:contact > 1/3, < 2/3 cortex thickness; grade 4:contact > 2/3 cortex thickness; grade 5:perforation; posterior deviations in grades 1 to 5 presented as minus).The theoretical distance was measured as if the anterior protrusive nail tip had been placed back to the central axis.The degree of the curvature and its corresponding radius were calculated by the geometric method. Results Of the 158 patients,the distal tip of PFNA- Ⅱ was located centrally along the axis of femur canal in 30 cases(19.0% ),posteriorly to the central axis in 10 cases(6.3% ),and anteriorly to the central axis in 118 cases (grades 1 to 3; 74.7% ),of which 55 cases experienced irritation caused by the contact between the distal tip and the anterior cortex (grades 2 to 3% ).The mean theoretical distance needed to put back the nail tip to the canal central axis was 1.42 ± 0.18 mm in the 17 cm nails,1.77 ± 0.39 mm in the 20 cm nails,and 2.46 ±0.20 mm in the 24 cm nails.Their corresponding curvature arcs were 2.51° ±2.40°,2.13° ± 1.65° and 2.09° ± 0.98°,and their curvature radii were 1483 ± 818 mm,2329 ± 1293 mm,and3710 ± 1957 mm,respectively. Conclusions There is a significant mismatch between the current short straight celphalomedullary nail (PFNA- Ⅱ) and the femoral anterior how in Chinese population.A curvature design is needed for the short cephaiomedullary nails to match the femoral anterior how of Chinese population.
7.Ultrasound Diagnosis of Carotid Atherosclerosis and Its Clinical Significance
International Journal of Cerebrovascular Diseases 2006;0(02):-
Carotid atherosclerosis is one of the important risk factors of ischemic cerebrovascular diseases. Ultrasonography can detect carotid intima-media thickness, compositions, configuration and surface structure of plaque, severity of carotid stenosis and its hemodynamics change, thus it is helpful to estimate the mechanism of ischemia and evaluate its risk factors. So it has a wide application prospect in screening high-risk population, guiding drug administration and surgical intervention.
8.The reason and the countermeasure of the deterioration of indoor air quality in medical district
Journal of Medical Postgraduates 2003;0(11):-
The deterioration of indoor air quality (IAQ) in medical district was caused by the personnel in the area, the buildings and their surroundings, and the situations of the air conditioning system. It is necessary to control the personnel entering the medical area and to strengthen the management of the buildings and their surroundings, and the air conditioning system for maintaining high IAQ. The development of new efficient air cleaning (equipment) is of importance in the same way.
9.Reticulocyte parameters of normal human and its relationship with mature erythrocyte parameters
International Journal of Laboratory Medicine 2016;37(8):1049-1051,1054
Objective To investigate the relationship between the parameters of the red blood cells and the corresponding param‐eters of mature red blood cells .Methods Blood samples of 112 healthy males and 110 healthy females were tested for these reticu‐locyte parameters by using Siemens Advia2120 hematology analyzer .Reference intervals were established .Comparisons between these reticulocyte parameters and the corresponding parameters of mature erythrocytes were drawn .Results Reference intervals for the healthy group recruited in this study were determined as follows .Females ,percentage of reticulocyte (Retic% ):0 .45% ~1 .76% ,absolute reticulocyte number(Retic#):(22~86)× 109/L ,mean corpuscular hemoglobin content of reticulocyte(CHr):30 . 1~36 .4 pg ,mean corpuscular volume of reticulocyte(MCVr):99 .5~113 .7 fL .Male ,Retic% :0 .54% ~1 .93% ,Retic# :(29~104)× 109/L ,CHr :32 .6~36 .6 pg ,MCVr :97 .6~112 .7 fL .The absolute reticulocyte of male is significantly higher than that of female;MCVr was significantly larger than mean corpuscular volume of mature erythrocytes ,which was also slightly larger in male than that in female;CHr was significantly higher than mean corpuscular hemoglobin of mature erythrocytes while mean corpuscular hemoglobin concentration of large reticulocytes was lower than that of mature erythrocytes;red blood cell volume distribution width of reticulocytes was lower than that of mature erythrocytes;the hemoglobin content distribution width of mature erythrocytes has a higher uniformity compared with reticulocytes .Conclusion The reference ranges of not commonly used parameters of reticulocytes were established ,and were compared with the corresponding parameters of red blood cells .
10.Discussion on Hospital Pharmaceutical Management Research in China
China Pharmacy 2001;0(10):-
OBJECTIVE:To improve the research level of hospital pharmaceutical management in China in order to pro-mote the pharmaceutical care in hospital.METHODS:298published articles on pharmaceutical management between2002and2003were collected and analyzed.The differences were compared between Chinese and American research papers.RESULTS&CONCLUSION:Chinese articles mainly dealt with drug management,including drug use,preparation management and drug purchase,and the research methods used in these articles were less scientific,while the research methodology in U.S studies is worthy of learning.