1.Roles of miRNAs in HPV-related cancers
Shimeng ZHANG ; Fei LIU ; Lei ZHENG ; Qian WANG
Chinese Journal of Laboratory Medicine 2014;37(12):977-981
High-risk human papillomavirus infection is the major cause of tumors such as cervical cancer and head and neck cancer.MicroRNAs (miRNAs) are 20-25nt small non-coding RNAs,which are involved in the proliferation,apoptosis,invasion and migration of tumors.HR-HPVs,which integrate into the host genome,encode three oncogenes:E6,E7 and E5 and enhance carcinogenesis by regulating downstream miRNAs and genes e.g.p53 and pRb.
2. Correlation analysis of gut microbiota and biochemical indexes in patients with non-alcoholic fatty liver disease
Shimeng REN ; Lu MEI ; Huang HUANG ; Shaofeng CAO ; Ruihao ZHAO ; Pengyuan ZHENG
Chinese Journal of Hepatology 2019;27(5):369-375
Objective:
To investigate the relationship between gut microbiota structure and biochemical changes in patients with different types of nonalcoholic fatty liver disease (NAFLD), in order to provide evidence for clinical diagnosis and prevention of NAFLD.
Methods:
Forty-eight NAFLD cases (NAFLD group), 40 NAFLD cases with type 2 diabetes mellitus (NAFLD combined with type 2 diabetes mellitus group) and 30 healthy cases (healthy group) were randomly enrolled, and their body mass index, serum alanine aminotransferase, aspartate aminotransferase, total bilirubin, total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein and uric acid were measured. Serum levels of TNF-alpha and fasting insulin were measured using ELISA, and then insulin resistance index was calculated. The gut microbiota of three groups of subjects was detected using 16S rDNA-based high-throughput sequencing. Lastly, the correlations between the various factors were analyzed. The comparison among groups was conducted by 2 test, and one-way ANOVA was used for comparison among groups with normal distribution and homogeneity of variance. Furthermore, the LSD method was used to compare the two groups. K-W rank sum test was used for comparison among groups without normal distribution or homogeneity of variance.
Results:
Body mass index, aspartate aminotransferase, triglyceride, total cholesterol, low density lipoprotein, uric acid, tumor necrosis factor-alpha, fasting insulin and insulin resistance index of NAFLD group were higher than healthy group, while the high-density lipoprotein was lower in the healthy group, and the difference was statistically significant (
3.Preliminary results of endoscopic stent placement for treating benign pyloric stenosis (with video)
Lixia ZHAO ; Shimeng ZHENG ; Dan LIU ; Lingjian KONG ; Deliang LI ; Qingfen ZHENG ; Yangyang ZHOU ; Ullah SAIF ; Huiyu YANG ; Bingrong LIU
Chinese Journal of Digestive Endoscopy 2021;38(6):483-486
From June 2017 to September 2019, 5 patients who were diagnosed as having benign severe pyloric stenosis underwent fully covered stent placement using a new stenting method at the First Affiliated Hospital of Zhengzhou University. Five patients were performed successfully without serious complications. Postoperative barium meal radiograph revealed that the stents were in good location and the acontrast agent passed smoothly. The liquid diet was commenced 1-3 days after surgery. No vomiting, abdominal pain and diarrhea occurred. During the follow-up, all the patients had improved weight and nutritional status. Four patients underwent stent removal 3-4 months postoperatively. One patient was found that the stent had migrated to stomach 3 months after discharge. After removing the stent, balloon dilation and mucosal resection was performed for the mild pyloric stenosis. All 5 patients had an additional follow-up of 3 months, and no symptoms and restenosis occured. These preliminary results showed that the new stenting method of fully covered stent placement is feasible, safe and effective in the treatment of benign pyloric stenosis.