1.Axillary Artery Perfusion in Arteriosclerotic Thoracic Aortic Aneurysm.
Tetsuro Uchida ; Takashi Minowa ; Jun Hosaka ; Masataka Koshika ; Kiyoshige Inui ; Takao Watanabe ; Yasuhisa Shimazaki
Japanese Journal of Cardiovascular Surgery 2002;31(4):266-268
Between 1996 to 2000, 12 patients with arteriosclerotic thoracic aortic aneurysm underwent surgery with cardiopulmonary bypass using the right axillary artery as an arterial inflow. All patients received total arch replacement with selective cerebral perfusion and deep hypothermic circulatory arrest. One patient with occlusion of the left carotid artery died of postoperative stroke. There were no postoperative complications or deaths related to axillary artery perfusion except for cerebrovascular accidents. Perfusion through the axillary artery, providing antegrade aortic flow, is a safe and effective procedure to avoid stroke owing to retrograde arterial perfusion. We believe that the axillary artery could be an alternative to conventional femoral artery cannulation in the setting of aortic arch operations.
2.Engineered three-dimensional rabbit oral epithelial-mesenchymal-muscular hybrid sheets
Yamane SHIGEKI ; Higa KAZUNARI ; Umezawa TAKASHI ; Serikawa MASAMITSU ; Shimazaki JUN ; Abe SHINICHI
International Journal of Oral Science 2016;8(3):145-154
Regenerative muscles are required for swallowing and mastication, and are important for functional recovery from diseases involving oral muscular defects. Therefore, we generated three-layer hybrid sheets, similar to oral mucosal structures containing submucosal muscles, using rabbit oral mucosa epithelial, mesenchymal, and myoblastic progenitor cells, and examined the structural proteins. Each cell type was obtained from rabbit oral mucosa using enzymatic digestion. Isolated mesenchymal and myoblastic cells were multi-differentiated into osteoblasts, adipocytes, and chondrocytes or myotubes. Isolated epithelial cells were cultured on collagen gels containing isolated mesenchymal cells for 2 weeks, and these epithelial–mesenchymal cell sheets were laminated onto myoblastic cell sheets. The engineered hybrid sheets were multi-stratified in the epithelial and myoblastic layers in a time-dependent manner, expressing intermediate cytoskeletal filament proteins of epithelium and muscle. Hybrid sheets also expressed extracellular matrix basement membrane proteins. Immature cell markers for epithelial and myoblastic cells were observed continuously in hybrid sheet cultures. We established engineered three-dimensional rabbit oral mucosa hybrid sheets containing each immature cell type in vitro.
3.Epiphrenic Esophageal Diverticulum
Akira HOKAMA ; Yoriyuki SHIMAZAKI ; Jun FUKUCHI ; Maki NISHIZAWA
Chonnam Medical Journal 2023;59(2):142-142