1.Studies on HPLC Fingerprint of Triterpene Acids in Poria Peel
Shaojun WANG ; Qi DUAN ; Shilin YANG ; Qianfeng GONG ; Jihua ZHANG
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(01):-
Objective To establish a method for HPLC fingerprint determination of the triterpene acids in poria peel.Methods Ethanol solvent and ultrasound method were used to extract the sample. The chromatographic conditions of RP-HPLC were:acefonitrile-0.5%phosphoric acid(linear gradient elution),the detection wavelength being 242 nm, temperature at 30℃and flow rate being 1.0mL?min-1. Ten batches of poria peel produced in different places were measured.The common model was set up to calculate the correlation coefficient, cos?between every batch vector and the total standard model vector, thus to define the similarity.Results There was much difference in the constituent content of poria peel produced in different places and batches.Conlusion The HPLC fingerprint of triterpene acids is stable and reprodacible,which is useful for standard cultivation and quality evaluation of the crud drug of poria peel.
2.Design of SpO_2 Measuring and Analysis System Based on LabVIEW
Shilin HE ; Guangfei CHEN ; Qi LI ; Jun YING
Chinese Medical Equipment Journal 2003;0(11):-
Objective To design and develop a kind of SpO2 detection and analysis system based on LabVIEW. Methods Pulse signal process and SpO2 calculation were achieved by designing a control system based on TI Company's MSP430FG439 MCU and using the digital signal process and graphic controls of the LabVIEW software. The design project and the realization way were given out by discussing the software of LabVIEW. Results The system could acquire, display and process pulse signal and calculate SpO2. Conclusion The system carries out a new way to design medical monitor equipment. Furthermore, it provides a shortcut for special analysis of the SpO2 and pulse signal in clinic.
3.Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity.
Yanbo ZHU ; Qi WANG ; Chengyu WU ; Guoming PANG ; Jianxiong ZHAO ; Shilin SHEN ; Zhongyuan XIA ; Xue YAN
Journal of Integrative Medicine 2010;8(11):1023-8
To explore the relationships between traditional Chinese medicine (TCM) constitutional types and overweight or obesity so as to provide evidence for adjusting constitutional bias and preventing and treating obesity.
5.Changes of endocrine and immune function in subjects of yang deficiency constitution.
Qi WANG ; Shilin YAO ; Jing DONG ; Hongdong WU ; Chengyu WU ; Zhongyuan XIA ; Hefeng SHI ; Guoming PANG ; Qiwei DENG ; Jianxiong ZHAO ; Jing CAI ; Zhengzhi CUI
Journal of Integrative Medicine 2008;6(12):1226-32
To investigate the changes of endocrine, cyclic nucleotide and immune systems in subjects of yang deficiency constitution, and to explore the relationship among characteristics and causes of yang deficiency constitution, the physiological and biochemical parameters.
6.Analysis of clinical features and genetic variants in a Chinese pedigree affected with tuberous sclerosis.
Haiyan MI ; Yongjun CHEN ; Shilin QI ; Xijin LIU ; Min LI ; Yang SHEN ; Shiyang LIU
Chinese Journal of Medical Genetics 2021;38(4):363-365
OBJECTIVE:
To analyze the clinical features of a Chinese pedigree affected with tuberculosis sclerosis and explore its molecular pathogenesis.
METHODS:
Clinical data of the proband and members of his pedigree were collected. Whole exome sequencing was carried out to detect variants of the TSC1 and TSC2 genes. Candidate variants was verified by Sanger sequencing and bioinformatic analysis.
RESULTS:
The proband and his mother, who also had mild features of tuberous sclerosis, were found to harbor a novel heterozygous c.4183C>T (p.Q1395X) variant of the TSC2 gene, which was absent in the 4 healthy relatives. Bioinformatic analysis suggested the variant to be likely pathogenic.
CONCLUSION
The heterozygous c.4183C>T (p.Q1395X) variant of the TSC2 gene probably underlay the disease in this pedigree. Above finding has expanded the spectrum of TSC2 gene variants. The more severe symptoms in the proband may be attributed to phenotypic heterogeneity of this disease.
China
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Humans
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Mutation
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Pedigree
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Tuberous Sclerosis/genetics*
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Tuberous Sclerosis Complex 2 Protein/genetics*