0. 05),while that in group C was significantly lower(P

Objective To evaluate DOG1 as an immunohistochemical marker for GIST.Methods From Jan 1987 to Sep 2008,210 consecutive cases including 137 GISTs as well as 73 non-GIST sarcoma were evaluated for clinicopathological characteristics and immunostained for DOG1 antibodies.Result Immunoreactivity for DOG1 was detected in 110 of 137 GIST cases (80.3%),3 out of 11 CD117negative GISTs were DOG1-positive.Only 1 of 73 non-GIST case was positive for DOG1.The expression of DOG1 in GIST is associated with the location of tumor,cell density,nuclear pleomorphism and Fletcher grading criteria.The postoperative 1-,3-,5-year overall survival for DOG1 negative and positive patients was 81.3%,74.5%,74.5% and 98.5%,85.9%,83.2%,respectively,P = 0.034.Conclusion DOG1 was a sensitive and specific marker for GIST in gastrointestinal mesenchymal tumors (GIMT).

Objective To assess apoptosis mediated by the cell death receptor Fas in peripheral T lymphocytes of patients with abdominal aortic aneurysm. Methods The apoptotic pathway was triggered by anti-Fas monoclonal antibody in cultured and activated peripheral T-cells from 20 AAA patients. Control groups consisted of 15 patients with aortic atherosclerotic occlusive disease(AOD)and 25 healthy individuals. Cell survival and death rate were assessed. Results Cross-linkage of Fas receptor exerted a strong apoptotic response on T cells from AOD patients and healthy controls, while the effect on T cells was very limited from that of AAA patients. The evaluation of cell Survival rate showed a significantly higher percentage in AAA group(98.9%±10.3%)than in the AOD subjects(58.9%±15.2%)or the healthy group(59.4%±12.9%;P＜0.001＝.Apoptosis assessment by annexin V and propidium iodide staining and flow cytometry showed similar results. The defect in AAA group was not due to decreased fas expressed at normal levels. Moreover,it specifically involved the Fas system because cell death was induced in the normal way by methylprednisolone. Conclusions Fas-induced apoptosis in activated T cell from AAA patients is impaired. This may disturb the normal down-regulation of the immune response and thus provide a new insight into possible mechanisms and routes in the pathogenesis of AAA.

Objective To compare the therapeutic efficiency of laparoscopic and open radical colorectal surgery in the treatment of colorectal carcinoma. Methods Clinical data of 77 cases undergoing laparoscopic colorectal surgery from September 2004 to October 2007 were compared with 90 patients treated by open surgery. Results Mean operating time was longer in the laparoseopic group than that in the open group [248 minutes vs. 225 minutes (t = -2. 11 ,P =0. 036)], blood loss was less in laparoscopic group [210 ml vs. 315 ml (t = 2. 82, P = 0. 005)]. Laparoscopic surgery was associated with lower rate of analgesia use [48% vs. 80% (x2 = 18. 69 ,P < 0. 01)], earlier recovery of bowel function [2. 9 days vs. 4. 3 days(t =5.59,P <0. 01)]and shorter hospital stay [12. 5 days vs. 15.5 days (t =2. 32,P=0. 039)]compared with open surgery. The number of removed lymph nodes [14. 2 vs. 15.3 (t = 1.04, P = 0. 3)]and length of reseeted bowel [18. 9 cm vs. 20. 0 cm, (t = 0. 88,P = 0. 383)]were not different between the two groups. The mean follow-up time of the two groups were 28 months, local recurrence rate, metachronous metastases rate and 3-year cumulative survival rate were not statistically different between the two groups. Conclusion Laparoscopic surgery is as effective as conventional open surgery in the treatment of colorectal carcinoma.

OBJECTIVETo study the relationship between glutathione S-transferase M1 (GSTM1) genotypes and lipid peroxidation of asbestos workers.

METHODS94 asbestos workers and 51 controls were selected as subjects. The general information, occupational history and individual habits were collected by questionnaires in all participants. The venous blood was sampled and the plasma was separated for the detection of malondialdehyde (MDA) level and lymphocytes for DNA isolation and GSTM1 genotyping.

RESULTSMDA level was significantly higher in asbestos workers [(0.283 +/- 0.054) nmol/L] than that in controls [(0.163 +/- 0.053) nmol/L, P < 0.01], however, neither duration of exposure nor accumulated asbestos exposure dose was related to MDA levels; MDA levels in control workers with GSTM1 +/- genotype [(0.190 +/- 0.034) nmol/L] were significantly higher than that in control workers with GSTM1 +/+ genotype [(0.138 +/- 0.055) nmol/L, P < 0.01]. Among asbestos workers, the same trend could be found, but the differences was not significant(P > 0.05). When the workers were stratified by duration of exposure or accumulated asbestos exposure dose, MDA levels in individuals with GSTM1 -/- genotype were also higher than those with GSTM1 +/+ genotype, but the differences were also not significant(P > 0.05).

CONCLUSIONBoth exposure to asbestos and deficiency of GSTM1 genotype were related to lipid peroxidation in workers, but the role of the former may be more important than that of the latter.

Asbestos ; adverse effects ; Genotype ; Glutathione Transferase ; genetics ; Humans ; Lipid Peroxidation ; Malondialdehyde ; analysis ; Occupational Exposure

Objective To investigate the regulatory effect of Xuebijing injection on inflammatory reaction during the preservation of isolated empty beating pig hearts with extracorporeal membrane oxygenation.Methods Twelve healthy Guangxi Bama miniature pigs were randomly divided into the Xuebijing group(n=6)and normal saline group(n=6).After the models were established in the Xuebijing group,Xuebijing injection was given at a dose of 5 mL/h through micropump in membrane oxygenator.In the normal saline group,an equivalent amount of 0.9％sodium chloride injection was pumped.Continuous pumping was performed for 8 h in both groups.The time of cardiac resuscitation and perfusion pressure,heart rate,perfusion flow rate after 8 h preservation were recorded in two groups.Pathological and ultrastructural changes of myocardial tissues in the left ventricular wall of hearts with cardiac arrest were observed after 8 h preservation.Serum levels of myocardial injury markers and inflammatory cytokines were detected in two groups at the beginning(T0),2 h(T2),4 h(T4),6 h(T6)and 8 h(T8)after model establishment,respectively.The expression levels of NOD-like receptor protein 3(NLRP3),cysteinyl aspartate specific proteinase-1(Caspase-1),apoptosis-associated speck-like protein containing a CARD(ASC)messenger RNA(mRNA)in myocardial tissues were measured at T0,T2,T4,T6 and T8,respectively.Results There were no significant differences in the time of cardiac resuscitation and perfusion pressure,heart rate,perfusion flow rate after 8 h preservation between two groups(all P＞0.05).Compared with the normal saline group,the levels of lactate dehydrogenase(LDH)at T4,creatine kinase(CK),LDH and α-hydroxybutyrate dehydrogenase(α-HBDH)at T6 and T8,tumor necrosis factor(TNF)-α at T4,T6 and T8,and interleukin(IL)-6,IL-18 and IL-1 β at T0,T2,T4,T6 and T8 were lower,and the mRNA relative expression levels of NLRP3 and Caspase-1 at T2,T4 and T6,and Caspase-1 and ASC at T8 were lower in the Xuebijing group,respectively(all P＜0.05).Hematoxylin-eosin staining and transmission electron microscopy showed that the degree of myocardial injury in the Xuebijing group was slighter than that in the normal saline group.Conclusions Xuebijing injection may effectively mitigate inflammatory response and exert certain myocardial protection effect during the ECMO preservation of isolated empty beating pig hearts.

Objective To investigate the effects of elderly hypertension with type 2 diabetes mellitus on cardiac autonomic nervous function and left ventricular structure and function.Methods The clinical data of 140 elderly outpatients with hypertension were retrospectively analyzed,and they were divided into diabetes group (n =38) and control group (n =102) according to situation of being complicated with type 2 diabetes mellitus.All patients underwent color Doppler echocardiography.The autonomic nerve functional indicators such as plasma neuropeptide Y (NPY),neurotensin (NT),left ventricular structure indicators such as left ventricular end diastolic diameter (LVEDd),left ventricular end systolic diameter (LVESD),interventricular septal thickness (IVST),left ventricular posterior wall thickness (LVPWT),left ventricular systolic function indicators such as left ventricular ejection fraction (LVEF),lefi ventricular shortening fraction (FS) and left ventricular diastolic function indicators such as mitral valve orifice diastolic early/late flow filling velocity (E/A)were observed and compared between the two groups when enrolled in the study.Results The plasma NPY level,LVEDd,LVESD,IVST,LVPWT and A level of diabetes group were significantly higher than that of control group when enrolled in the study (P ＜ 0.05 or P ＜ 0.01) while the plasma NT level,left ventricular systolic function indexes such as LVEF,FS and part of the left ventricular diastolic function indexes such as E,E / A were significantly lower than that of the control group (P ＜ 0.05 or P ＜ 0.01).Conclusion Elderly hypertension combined with type 2 diabetes mellitus have a greater effect on cardiac autonomic nervous function,left ventricular structure and left ventricular function,so it needs great attention.

Objective To investigate the effects of elderly hypertension with type 2 diabetes mellitus on cardiac autonomic nervous function and left ventricular structure and function.Methods The clinical data of 140 elderly outpatients with hypertension were retrospectively analyzed,and they were divided into diabetes group (n =38) and control group (n =102) according to situation of being complicated with type 2 diabetes mellitus.All patients underwent color Doppler echocardiography.The autonomic nerve functional indicators such as plasma neuropeptide Y (NPY),neurotensin (NT),left ventricular structure indicators such as left ventricular end diastolic diameter (LVEDd),left ventricular end systolic diameter (LVESD),interventricular septal thickness (IVST),left ventricular posterior wall thickness (LVPWT),left ventricular systolic function indicators such as left ventricular ejection fraction (LVEF),lefi ventricular shortening fraction (FS) and left ventricular diastolic function indicators such as mitral valve orifice diastolic early/late flow filling velocity (E/A)were observed and compared between the two groups when enrolled in the study.Results The plasma NPY level,LVEDd,LVESD,IVST,LVPWT and A level of diabetes group were significantly higher than that of control group when enrolled in the study (P ＜ 0.05 or P ＜ 0.01) while the plasma NT level,left ventricular systolic function indexes such as LVEF,FS and part of the left ventricular diastolic function indexes such as E,E / A were significantly lower than that of the control group (P ＜ 0.05 or P ＜ 0.01).Conclusion Elderly hypertension combined with type 2 diabetes mellitus have a greater effect on cardiac autonomic nervous function,left ventricular structure and left ventricular function,so it needs great attention.

Myopia,as one of the most prevalent eye diseases in the world,has become an important public health problem.In addition to damaging eye health,myopia also negatively affects students'academic performance,interpersonal interactions,and quality of life,leading to mental health problems such as anxiety and depression.In recent years,schol-ars at home and abroad have been paying more and more attention to the mental health status of myopic people,but how to objectively and accurately assess the mental health of myopic people is still an urgent problem to be solved.In this paper,we summarize the current situation and characteristics of the application of mental health scales for myopic people in do-mestic and international studies,analyze the applicability of the scales,and provide references for the scientific selection of myopic mental health scales.

OBJECTIVETo understand the effects of exposure to asbestos and GSTM1 genotypes on plasma activity of glutathione S-transferases (GSTs).

METHODSNinety-four workers exposed to asbestos and 51 controls were selected, and their general information, occupational history and personal behavior were collected by questionnaire. Venous blood specimen was collected from each of them and plasma was separated for detection of GSTs activity and lymphocytes for DNA extraction and GSTM1 genotyping.

RESULTSPlasma activity of GSTs in the asbestos-exposed workers (23.0 +/- 6.9) U/L was significantly lower than that in the controls (32.6 +/- 11.8) U/L, which declined with the length of employment in asbestos industry and the increase of cumulated dose of asbestos. Stratification of workers by GSTM1 genotypes showed that plasma activity of GSTs in asbestos-exposed workers with GSTM1+/+ or GSTM1-/- were (24.0 +/- 6.1) and (22.5 +/- 7.3) U/L, respectively, lower than those in the controls with the same genotypes (38.1 +/- 13.2) and (26.8 +/- 6.6) U/L. Plasma activity of GSTs in the control workers with GSTM-/- was significantly lower than in those with GSTM+/+, and, so did in asbestos-exposed workers, but without statistically significant difference.

CONCLUSIONExposure to asbestos could significantly decrease plasma activity of GSTs, and GSTM1 genotypes could affect on the activity of GSTs in the control workers, which was not so obvious in asbestos-exposed workers.

Adult ; Asbestos ; adverse effects ; Gene Frequency ; Genotype ; Glutathione Transferase ; blood ; drug effects ; genetics ; Humans ; Middle Aged ; Occupational Exposure ; adverse effects ; Time Factors