1.Correlation of adaptive skills with intelligence in young children
Chinese Journal of Tissue Engineering Research 2001;5(9):45-46
Objective To explore the relationship between adaptive skills and intelligence in young children. Method 30 young children aged from 3 to 6 years were concurrently administered the Adaptive Skill Rating Scale for Young Children(ASRSYC) and China- Wechsler Young Chilren Scale of Intelligence(C -WYCSI). Result The correlation coefficients between the total score of ASRSYC and the verbal scale score , performance scale score and full scale score of C - WYCSI were 0. 85, 0.70and 0. 81, respectively. The corrdlations between the total score of ASRSYC and the subtest scores of C - WYCSI ranged from 0. 51 to 0. 75 . Conclusion Adaptive skills and intelligenoe in young children relate each other and develop jointly.
2.Effects of blood transfusion on postoperative infection in patients with breast cancer
Xianqing ZHANG ; Ruoquan YAO ; Shijie MU
Journal of Clinical Surgery 2000;0(06):-
Objective To investigate the effects of blood transfusion on postoperative infection in patients with breast cancer.Methods 311 patients who underwent breast cancer operations in our hospital were observed.Among them,109 patients were transfused with red blood cell (RBC) concentrates,112 with white blood cell(WBC)-depleted RBC concentrates,90 were not transfused.The rates of postoperative infection of three groups were compared.Results The rate of postoperative infection of the group transfused with RBC concentrates was 5.50%.The rate of postoperative infection of the group transfused with WBC-depleted RBC concentrates was 0.89%,which was significantly different from that of the group transfused with RBC concentrates(P0.05).Conclusions In patients with breast cancer,the rate of postoperative infection of the group transfused with RBC concentrates was higher than that of group transfused with WBC-depleted RBC concentrates and of the group without transfusion. The rate of postoperative infection was closely correlated to the amount of RBC concentrates.
3.Relationship between inflammatory cytokines and traumatic brain injury
Zhansheng ZHU ; Shijie CHEN ; Mingcan WU ; Yuan YAO
International Journal of Surgery 2010;37(10):700-702
A medical studies have indicated that there would be a defense response from human body after TBI,which mainly comes with inflammatory reaction. Human body's resistance seems likely to be enhanced,during this process, by inflammatory cytokines leading to rehabilitation of the cellular organization.However,a strong Inflammation from the whole human body may be caused due to the excessive activation,which, instead, worsens secondary brain injury. This article discusses the relationship between cytokines and traumatic brain injury.
4.Expression of Notch4 in renal cell carcinoma and its relationship with the microvascular density
Liang PANG ; Guangming LIU ; Wenli SONG ; Zhen JING ; Shijie YAO
The Journal of Practical Medicine 2017;33(15):2525-2529
Objective To investigate the expression of Notch4 protein and to analyze its correlation with the clinical parameters and the microvessel dentisty in renal cell carcinoma. Methods The expression of Notch4 was examined in 60 cases of renal cell carcinoma and the para-carcinoma tissue by SP immunohistochemical stain-ing ,and CD34 detection was used for counting microvessel density. Statistical analysis was performed to reveal the correlation with clinicopathological parameters ,microvessel density and prognosis. Results The positive rate of Notch4 protein expression was 75%(45/60)in para-carcinoma tissue,and was 43.3%(26/60)in renal cell car-cinoma,with significant difference on tumor grade and Lymph node metastasis(P<0.05). The microvessel densi-ty in Notch4 positive tissues was significant lower than that in the negative samples(P<0.05). The survival time of patients with Notch4 positive expression was significantly longer than that of patients with Notch4 negative expres-sion(P<0.05). Conclusion Notch4 protein plays an important role in the development of renal cell carcinoma. Notch4 expression might both attenuate the malignant biological characteristics and suppress the angiogenesis dur-ing tumor development.
5.Significances of Up-expressions of CYCLIND1 and CDK4 in pancreatic carcinoma
Yong YANG ; Yunning HUANG ; Gang SU ; Shijie YANG ; Baoguo YAO
Basic & Clinical Medicine 2006;0(07):-
Objective To investigate the expressions of CYCLIND1 CDK4 in pancreatic cancer and their relationship with clinlicopathological parameters.Methods Immunohistochemical detector with new second generational tow steps method was used to detect the expressions of CYCLIND1 CDK4 and PCNA in 48 cases of pancreatic cancer and 14 cases of chronic pancreatitis.Results The expressions of CYCLIND1 CDK4 proteins in pancreatic cancer were 70.85%(34/48)and 62.50%(30/48) respectively.The CYCLIND1 and CDK4 proteins were increased(P
6.Study on mutation of mitochondrial gene from rat breast cancer
Pingzhong WANG ; Shijie MENG ; Pei LIU ; Yangzhen YAO
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To know the variations of the cytochrome b gene in cancer tissue, paracarinoma tissue and normal tissue and to inquire into the relationship between mutations of mitochondrial genome and carcinogenesis. METHODS: Cellular total DNA was extracted.The cytochrome b genes of three tissues were amplifyed with polymerase chain reaction(PCR). PCR products were analysed by DNA auto-sequencing method. RESULTS: The cytochrome b gene of cancer tissue had the C to G mutation at nt 14931, the C to G mutation at nt 15004 and the T to C mutation at nt15435,respectively. The cytochrome b gene of paracarinoma tissue had the A to C mutation at nt 15436. The cytochrome b gene of normal tissue had not mutation. CONCLUSION: Mitochondrial DNA mutations could be the endogenous factors that induce nuclear genome mutation. It could promoto carcinogenesis. The paracarinoma tissue was abnormal in DNA molecular level.
7.Development of urothelial tumors following renal transplantation of 11 cases report
Wenhui SONG ; Zhijie BAI ; Shijie YAO ; Qian HU ; Haifeng WANG ; Qingtong MA ; Shiqiang YANG ; Hongshun MA
Clinical Medicine of China 2012;28(5):528-530
Objective To analyze the incidence and clinical features of urothelial tumors in renal allograft recipients.Methods A retrospective analysis of 1042 patients received renal allografts who had taken immunosuppression for at least six months between 2006 and 2011 in The First Centre Hospital of Tianjin was performed.Results Eleven cases of uroepithelial tumors were diagnosed in the 1042 cases of renal transplantation ( 1.06% ),of whom 9 cases were noticed by hematuria ( 81.8 % ),2 cases ( 18.2% ) by medical examination.Six patients were diagnosed with multifocal urothelial carcinomas.Surgery was performed on all the patients with renal tumors and followed by chemotherapy or radiotherapy.Conclusion Malignancies in urinary tract after renal transplantation should be bore in mind.Early diagnosis is very important.The treatment options include reducing immunosuppressive agents and removing tumor lesions completely.
8.Evaluation of left ventricular systolic and twist function in patients with heart transplantation using ultrasound speckle tracking imaging
Cuizhen PAN ; Hao CHEN ; Xianhong SHU ; Minmin SUN ; Shijie ZHU ; Haohua YAO ; Chunsheng WANG
Chinese Journal of Ultrasonography 2008;17(11):930-933
Objective To investigate the feasibility of ultrasound speckle tracking imaging(STI)in assessing left ventricular(LV)systolic and twist function in patients with heart transplantation.Methods Nine patients with heart transplantation and 10 subjects with normal LV function were examined by GE Vivid 7 with a M3S probe.Eighteen regional peak systolic strain and displacement in longitudinal,radial,circumferential,and 18 regional degree of rotation were measured respectively.Results The segmental regional peak systolic strain and displacement were less than those in subjects with normal LV function(P <0.05),but segmental regional degree of rotation was more than that in subjeets with normal LV function (P<0.05).Conclusions STI may provide a noninvasive,simple and quick tool for evaluation of left ventricular systolic and twist function in patients with heart transplantation.
9.Comparison of phenotype,genotype and drug resistance genes of Acinetobacter baumannii isolated from clinic
Min WANG ; Meimei WANG ; Di YAO ; Xianping LI ; Hong CAO ; Zhangshun QIN ; Shijie DU ; Haiyan ZENG
Chinese Journal of Microbiology and Immunology 2010;30(9):821-828
Objective To investigate the genotypes and encoding resistance genes differences of Acinetobacter baumannii and analyze their interrelations with multi-drug resistance.Methods A total of 77strains Acinetobacter baumannii were collected random from the second Xiangya Hospital during September 2008 to September 2009.The K-B method which was WHO recommended was adopted to Acinetobacter baumannii drug sensitivity test to 15 kinds of antibiotics to establish susceptibility spectrum.At the same time,random amplified polymorphic DNA(RAPD)technique was used to establish DNA fingerprinting.The genes of β-lactamase(TEM-1,IMP,OXA-23,OXA-24,AmpC),aminoglycoside-modifying enzymes[aac(3')-Ⅰ ,aac(6')-Ⅰ ,ant(3")-Ⅰ]and 16S rRNA methylase(armA,rmtA,rmtB)were detected by PCR and sequenced,and find the relationship between the gene encoding and multi-drug resistance.In addition,we compared the rates of resistance genes of Acinetobacter baumannii and the relations with the genotype and the multi-resistance.Results Thirty-one sensitive strains and 46 multi-drug resistance strains(10 Pan-drug resistances)were isolated.Seventeen types from A to Q were separated using RAPD technique.E genotype widely popular in the ICU was the advantage type in multi-drug resistance strains,and the rate was 47.1%.While the various types scattered in sensitive strains.The positive rates of TEM-1,IMP,OXA-23,OXA-24,AmpC,aac(3')-Ⅰ ,aac(6')-Ⅰ ,ant(3")-Ⅰ ,armA in the multi-drug resistance strains and the sensitive strains were 95.7%,39.1%,84.8% ,54.3%,87.0%,89.1%,84.8%,45.7%,63.0% and 58.1%,9.7%,32.3%,48.4%,48.4%,29.0%,45.2%,12.9%,9.7%,respectively,and there was significant difference except for OXA-24 using the X2 test(P < 0.05).All isolates were negative for rmtA gene and rmtB gene.Drug susceptibility analysis showed that the resistant rate was significantly higher of the strains carrying resistant genes than that of the resistance negative strains.When the strains were resistant to gentamicin and amikacin,the rate of three aminoglycoside genes positive was 34.8%.The trains containing all the measured β-lactamase genes were all resistant strains.Conclusion Compared with the sensitive Acinetobacter baumannii strains,a broad resistance spectrum and a high drug resistance rate were showed in multidrug resistance strains isolated from clinic,which harboring many kinds of β-lactamase genes and aminoglycosides genes with a high separation rate,and the same clone of multiple drug-resistant strains may be transmitted in and among wards.
10.Expression of Oct4 and Wnt2 in human glioma tissues and its clinical significance
Guangrui ZHAO ; Mingcan WU ; Shijie CHEN ; Junchuan LI ; Tingxuan CHEN ; Yuan YAO
Chinese Journal of Cancer Biotherapy 2009;16(6):629-632
Objective:To investigate the expression of Oct4 and Wnt2 in human glioma tissues and its relationship with the clinicopathological features of glioma. Methods: Fifty-six paraffin blocks were obtained from glioma patients receiving surgery. The diagnosis of these patients were confirmed by pathology in our hospital from 2006-2009. Immunohistochemi-cal staining was used to examine Oct4 and Wnt2 expression in the brain tissues of 10 patients with acute brain injury and 56 glioma tissues (including 15 recurrent cases). Results: The normal brain tissues were negative of Oct4, with only one case showing weak Wnt2 expression. Thirty-four of the 56 glioma tissues showed positive expression of Oct4 (60.7%), and 40 showed positive expression of Wnt2 (71.4%). Positive expression rates of Oct4 and Wnt2 in low-grade and high-grade glioma tissues were 46.2 %, 73.3% and 57.7 %, 83.3%, respectively (P < 0.05). Oct4 positive rates in the recrudescence and newly diagnosed glioma tissues were 86.7% and 51.2%, respectively (P < 0.05). Oct4 expression in the glioma tissues was positively correlated with that of Wnt2 (r = 0.537, P < 0.01). Conclusion: Expression of Oct4 and Wnt2 is associated with the malignant degrees of glioma, and Oct4 expression is related to the recurrence of glioma. Oct4 might participate in the development and progression of brain glioma through Wnt signaling pathway.