1.An Investigation of Indoor Air Pollution in Newly Decorated Rooms in Luohe
Shijie LI ; Xiaojie ZHANG ; Wen ZHENG
Journal of Environment and Health 1993;0(01):-
Objective To investigate the indoor air pollution caused by construction materials and decoration materials and present scientific data for controlling indoor air pollution caused by construction and decoration materials. Methods 126 samples collected from 44 newly decorated rooms in some districts of Luohe city. The levels of ammonia, benzene and formaldehyde were analyzed according to the national standard. Results The rates of exceed standard of benzene and formaldehyde concentration were 8.7% and 72.2% respectively. Formaldehyde concentration was 3.4 times of the national standard limit averagely. Ammonia and dimethylbenzene were not detected. Conclusion Some decoration materials can pollute the indoor air. The formaldehyde and benzene concentration tend to decrease as time goes on.
2.The comparison of methodologies ot assessing myocardial reperfusion in patients with acute myocardial infarction after primary angioplasty
Shijie ZHAO ; Wen TIAN ; Guoxian QI ; Yaming LI ; Xuena LI
Chinese Journal of Postgraduates of Medicine 2008;31(28):19-21
Objective To assess the predictive value of coronary angiography TIMI myocardial perfusion grade (TMPG), electrocardiogram (ECG) single-lend ST segment resolution (STR), ECG Max-ST-segment deviation (MaxSTE) on judging myocardial reperfusion after primary angioplasty in the patients with acute myocardial infarction (AMI). Methods Primary percutaneous coronary intervention(PCI) was performed in 42 patients within 12 hours after AMI onset. Coronary angiography and ECG was done before and after angioplasty. TMPG, ECG single-leed STR and MaxSTE were used to assess myocardial reperfusion immediately after PCI. Myocardial perfusion scan was examined in all the patients using99mTc-MIBI SPECT on day 7±2. Results Compared with the level of myocardial perfusion demonstrated in myocardial scan of 99mTc-MIBI SPECT, the sensitivity, specificity and accuracy of TMPG, single-lead STR and MaxSTE was calculated. The sensitivity of TMPG, single-lead STR and MaxSTE was 93.75%, 87.50% and 81.25%, respectively, the specificity of them was 20.00%, 80.00% and 80.00%, respectively, and the accuracy was 76.19%, 85.71% and 80.95%, respectively. The findings in single-lead STR and MaxSTE matched well with the results of myocardial scan of 99mTc-MIBI SPECT. Conclusion In the patients suffering from AMI treated with PCI, ECG is an effective method to assess myocardial tissue reperfusion.
3.Incidence rates of cancer among registered residents of urban Shijiazhuang in 2012
Denggui WEN ; Nan ZHANG ; Cheng FENG ; Baoen SHAN ; Shijie WANG
Chinese Journal of Clinical Oncology 2015;(3):141-146
Objective:To study the incidence rates of cancer in the urban area of Shijiazhuang city, China in 2012 based on the data of 2,374,827 registered residents. Methods: The incidence of diagnosed cancer cases in 2012 was obtained from the hospital reimbursement database of the medical insurance center of the city by retrieving the records on first-time reimbursement applications for the hospitalization of tumor patients from January 1 to December 31 in 2012. Population census data was obtained from the Population Department of the Shijiazhuang Public Security Bureau. The site-specific and sex-specific age-adjusted incidence rates were calculated. Results:The overall incidence rate, the age-adjusted rate of the Chinese population (ASRC), and the age-adjusted rate of the world population (ASRW) for both men and women were 237.53, 129.86, and 167.71 per 100,000 individuals, respectively. The incidence rate increased with age and peaked in the 75-79 years age groups of men and women at 1,729.42 and 867.35 per 100,000 individuals, respectively. The top ten most frequently diagnosed cancers in males were lung, stomach, colorectal, liver, esophagus, kidney, prostate, leukemia, bladder, as well as lymphoma, whereas those in females were the breast, lung, colorectal, stomach, cervical, uterine body, ovary, lymphoma, esophageal, and liver cancers. The incidence rate and ASRW of all cancers combined in men were 269.05 and 187.52 per 100,000 individuals, whereas those for women were 207.57 and 150.44 per 100 000 individuals, respectively. Compared with the average incidence rates of 31 Chinese cities in 2009, the ASRW of lung, stomach, and colorectal cancers in males from Shijiazhuang was nearly equal to the national level;however, the ASRW of breast cancer in females from Shijiazhuang was higher than the national level. When the incidence rates of Shijiazhuang in 2012 were compared with those of Beijing in 2009, the ASRW of stomach and esophageal cancers in men of Shijiazhuang was twice that of the same cancers in Beijing. However, the same parameters for the pancreatic and prostate cancers in men, as well as the thyroid and uterine body cancers in women of Beijing, were twice the values for Shijiazhuang. Conclusion: The ASRWs of the major types of cancer, such as the lung, stomach, colorectal, and breast cancers, in urban Shijiazhuang in 2012 were identical to those of the 31 Chinese cities in 2009. Compared with Beijing, the incidence rates of pancreatic, prostate, and thyroid cancers were significantly higher in Shijiazhuang, whereas those of esophageal and stomach cancers were significantly lower.
4.Simultaneous Determination of 6 Antidepressants in Human Plasma by HPLC
Youmin WEN ; Shijie WEI ; Hongwan DANG ; Xiaoying YANG ; Lechuan JIA ; Weihong SUN
China Pharmacy 2007;0(32):-
OBJECTIVE:To establish a rapid and sensitive HPLC method for simultaneous determination of plasma concentrations of 6 antidepressants.METHODS:With diprozin as internal standard,the alkalized samples were extracted by liquid-liquid extraction and separated on a Phenomenex-C18 column using acetonitrile-0.05 mol?L-1 sodium dihydrogen phosphate(pH was adjusted to 2.5 by phosphoric acid) as mobile phase at a flow rate of 1.2 mL?min-1 by a gradient elution.The column temperature was set at 40℃.Venlafaxine was detected by fluorescence detection at an Ex wavelength of 276 nm and Em wavelength of 598 nm;doxepin,paroxetine,sertraline,fluoxetine and amitriptyline were detected at an UV detection wavelength of 200 nm.RESULTS:The linear ranges of venlafaxine,doxepin,paroxetine,sertraline,fluoxetine and amitriptyline were 5~1 000 ?g?L-1,40 ~200 ?g?L-1,20~800 ?g?L-1,40~1 000 ?g?L-1 and 10~400 ?g?L-1,respectively,with correlation coefficients ≥0.990 for all.Both the intra-day RSD and inter-day RSD were less than 15%;the extraction recovery rates were greater than 60% and methodological recovery were greater than 90% for all the samples.CONCLUSION:The method is simple,economical,rapid,accurate and sensitive,and it is applicable for the clinical monitoring of plasma drug concentrations as well as the analysis and pharmacokinetic study of toxic drugs.
5.Effect of hand hygiene comprehensive intervention on incidence of pediatric healthcare-associated infection
Weixiu YANG ; Ping ZHENG ; Hua QIN ; Huacheng JIN ; Shijie LIU ; Yurong DAI ; Shixiang WEN ; Xiaoyan WANG ; Xiuli ZHANG
Chinese Journal of Infection Control 2017;16(4):297-302
Objective To explore the effect of 5M1E quality analysis tool-based hand hygiene(HH) comprehensive intervention measures on pediatric healthcare-associated infection(HAI).Methods HH status of 29 health care workers(HCWs) in the pediatric department of a hospital was monitored, April-June 2015 was baseline survey stage, 5M1E quality analysis tool was adopted to analyze various factors affecting the compliance rate of pediatric HH, intervention measures began to be taken in June 2015, and compared with HH in July 2015-March 2016.The occurrence of HAI between July 2014-March 2015 and July 2015-March 2016 was compared.Results HH compliance rate of HCWs increased from 30.86% in April-June 2015 to 81.94% in January-March 2016;HH correct rate increased from 68.14% to 93.75%;HH compliance rate and correct rate of HCWs gradually increased(X2=2 608.626, 630.798,respectively, both P<0.001).Qualified rate of detection of HCWs' HH sampling increased from 20.69% in April-June 2015 to 89.66% in January-March 2016(X2=31.957,P<0.001).Incidence of HAI decreased from 7.74% in July 2014-March 2015 to 3.62% in July 2015-March 2016(X2=46.717,P<0.001).Conclusion Application of 5M1E quality analysis tool in the investigation, analysis, and comprehensive intervention in HCWs' HH in pediatric department has greatly enhanced HCWs' HH compliance rate, and reduced the incidence of HAI in pediatric patients.
6.Clinical Observation of Fire-needle Acupuncture for Sub-acute and Chronic Eczema
Yang CHENG ; Xiaoyong ZHOU ; Xianyu ZENG ; Jiyuan WU ; Wen LIU ; Liming TIAN ; Kai LI ; Qingjun TIAN ; Shijie BAO
Shanghai Journal of Acupuncture and Moxibustion 2014;(10):903-905
Objective To compare the therapeutic efficacies of fire-needle acupuncture and Halometasone cream in treating sub-acute and chronic eczema. Method Totally 114 patients were divided into a treatment group and a control group. The treatment group was intervened by using fire-needle acupuncture, and the control group was by Halometasone cream. Itchy score and Eczema Area and Severity Index (EASI) were used for comparison before and after intervention. Result There were significant differences in comparing the markedly-effective rate and total effective rate between the fire-needle acupuncture group and the Halometasone control group according to the itchy score (P<0.01). There were no significant differences in comparing the markedly-effective rate and total effective rate between the two groups according to EASI (P>0.05). Conclusion Fire-needle acupuncture is superior to Halometasone cream for acute and chronic eczema in relieving itch, action time and effect-sustaining duration.
7.The study on Norovirus infection status among patients with virus diarrhea in Xiamen district
Huineng ZHENG ; Li LI ; Juanjuan CHEN ; Shijie HUANG ; Guilin MA ; Huixin WEN ; Litong SHEN ; Changyi ZHOU ; Jianwei HUANG
Chinese Journal of Zoonoses 2010;(3):259-262
The purpose of the present study was to investigate Norovirus infection status among patients with virus diarrhea in Xiamen city and provide evidence for exploring the prevalence characteristics and constituting appropriate control strategy.From April 2007 to July 2008,323 fecal samples of virus diarrhea cases collected from 3 surveillance hospitals in Xiamen were detected for antigen and RNA by ELISA and Real-Time RT-PCR respectively.The RdPd genes from some samples were furtherly amplified and sequenced for genogroup identification when the Real-Time RT-PCR detection results were positive.In the 323 fecal specimens,68 (21.05%) were positive for Norovirus antigen by ELISA and 107 (31.13%) were positive for Norovirus RNA by Real-Time RT-PCR.The overall positive prevalence rate of Norovirus in Xiamen was 38.08%.107 positive specimens were detected by Real-Time RT-PCR and results showed that 80 strains were Norovirus GGⅡ(74.77%),2 strains were GGⅠ(1.87%) and 25 strains (23.36%)were unidentified.It's indicated that Norovirus infection in Xiamen district was mainly caused by Norovirus GGⅡ,and Norovirus was also the main cause for virus diarrhea.
8.RovoIving ultrasonic probe for medical endoscope imaging system based on USB2.0.
Xiaodong CHEN ; Shijie WEN ; Daoyin YU
Journal of Biomedical Engineering 2008;25(5):1048-1052
This paper mainly introduces the design and implementation of rotary scanning imaging for Endoscopic Ultrasonography System based on USB2.0. The ultrasonic pulse-echo imaging technique has been used. In the paper, we simply introduce the emission circuit, receiving circuit and isolation circuit. According to the character of rotary scanning, we design the synchronous control circuit based on FPGA and data transmission circuit based on USB2.0. Finally the original polar coordinate image is transformed to rectangular coordinate grey image through coordinate transformation. The system performances have been validated by the experimental result gotten by scanning a specific object with continuous rotary motor.
Capsule Endoscopes
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Endoscopy, Gastrointestinal
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methods
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Equipment Design
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Humans
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Image Processing, Computer-Assisted
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methods
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Ultrasonography
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instrumentation
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methods
9.Coded excitation circuit on medical ultrasonic endoscope imaging system.
Daoyin YU ; Jing BAO ; Xiaodong CHEN ; Shijie WEN
Journal of Biomedical Engineering 2009;26(3):484-487
In this paper, we introduce a coded excitation circuit for medical ultrasonic endoscope imaging system. This circuit is composed of TC6320 and its drive chip. The experiment demonstrates that this circuit can export frequency-adjustable, duration-controllable coded signals, and its voltage reaches +/-60 V. It still works well when excitant frequency reaches 30 MHz. Compared with the conventional medical ultrasonic pulse-echo imaging system, this coded excitation system has the potential of higher SNR and deeper penetration depth, especially for the small emission power system. This method has a bright future.
Endosonography
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instrumentation
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methods
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Equipment Design
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Humans
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Image Interpretation, Computer-Assisted
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methods
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Transducers
10.Development of a rapid molecular typing method for Vibrio cholerae using melting curve-based multilocus melt typing.
Shijie HUANG ; Zanzan LIU ; Huixin WEN ; Li LI ; Qingge LI ; Jianwei HUANG
Chinese Journal of Preventive Medicine 2015;49(2):122-127
OBJECTIVETo develop a high-throughput rapid method for Vibrio (V.) cholerae molecular typing based on Melting Curve-based Multilocus Melt Typing (McMLMT).
METHODSSeven housekeeping genes of V.cholerae were screened out, and for each gene, the specific primers were designed for correspondent genes as well as 4 probes covering polymorphism loci of sequences. After optimizing all parameters, a method of melting-curve analysis following asymmetric PCR was established with dual-fluorescent-reporter in two reaction tubes for each gene. A set of 28 Tm-values was obtained for each strain and then translated into a set of code of allelic genes, standing for the strain's McMLMT type (MT). Meanwhile, sequences of the 7-locus polymorphism were typed according to the method of MLST. To evaluate the efficiency and reliability of McMLMT, the data were compared with that of sequence-typing and PFGE using BioNumerics software.
RESULTSMcMLMT method was established and refined for rapid typing of V. cholerae that a dozen of strains can be finished testing in a 3-hours PCR running using 96-well plates. 108 strains were analyzed and 28-Tm-values could be grouped and encoded according to 7 housekeeping gene to obtain the code set of allelic genes, and classified into 18 types (D = 0.723 3). Sequences of the 7 genes' polymorphism areas were directly clustered into the same 18 types with reference to MLST method. 46 of the strains, each represented a different PFGE type, could be classified into 13 types (D = 0.614 5) with McMLMT method and A- K groups at 85% similarity (D = 0.858 9) with PFGE method.
CONCLUSIONMcMLMT method is a rapid high-throughput molecular typing method for batches of strains with a resolution equal to MLST method and comparable to PFGE group.
Multilocus Sequence Typing ; Polymerase Chain Reaction ; Vibrio cholerae