ObjectiveTo study the effect of different treatment on prolapse of lumbar intervertebral disc. Methods 169 patients with prolapse of lumbar intervertebral disc were divided into two groups, the contral group treated with traction and massage, and the comprehensive group treated with acupuncture, physical therapy, and phsiotherapy besides traction and massage. The assessment included index and rate in improvement and efficiency of rehabilitation. Results The index and rate in improvement and efficiency of rehabilitation is better in the comprehensive group than in the contral group. The course of treatment in the comprehensive group is shorter than that in the contral group.

Aim To investigate the effects of angiotensin-converting enzyme inhibitor Ena on proliferation and cell cycle protein of cardiac fibroblasts and to explore the mechanism of Ena on cardiac fibrosis.Methods CFb was isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,collagen synthesis was observed by hydroxyproline concentration method,flow cytometry,immunofluorescenic and Western blot was used to measure cell cycle and CKI p27kip1 with Ena.Results Ena decreased MTT value and collagen synthesis dramatically;Ena increased phase G0/G1 and decreased phase S percentage ratio in cell cycle;Ena enhanced p27kip1 protein expression in a dose-dependent manner.Conclusion The antiproliferative effects of Ena on CFb can be attributed to upregulating CKI p27kip1 protein expression.

Aim To investigate the effects of enalapril(Ena) on cardiac fibrosis induced by isoprenaline(Iso) and to explore its mechanism.Methods Effect of Ena and captopril(Cap) on collagen content,HW/BW,LVI,hydroxyprolnie(Hyp) level,and TGF-?1 protein expression was observed with IH and WB in rats induced by Iso subcutaneous injection.Results Different doses of Ena could decrease HW/BW,LVI and Hyp level dramatically,and decrease TGF-?1 protein expression which was closely related to myocardial fibrosis(MF).Conclusion Enalapril can inhibit TGF-?1 protein expression,by which inhibit myocardial fibrosis.

Objective To observe the effects of ACE inhibitor enalaprilat(Ena) and protein kinase C(PKC) inhibitor chelerythrine(Chele)on proliferation,collagen Ⅰ,cell cycle,PKC and cyclinD1 protein expression of neonatal cardiac fibroblasts(CFb) and to probe its molecular mechanism.Methods CFb of neonatal Wistar rats were divided into control group,AngⅡ group,Chele+AngⅡ group,Chele+AngⅡ+Ena group and AngⅡ+Ena group.CFb were isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,immunocytochemical staining(IC) was used to measure collagen Ⅰ content,Western blotting and flow cytometry were used to detect PKC,cyclinD1 and cell cycle respectively.Results Compared with AngⅡ group,the MTT value decreased dramatically(P

Objective:To study the relationship betwen the mechanism of autoimmune disease and CD4+CD25+ T cell population in NIK mutated mice-aly mice.Methods:NIK mutated mice-aly/aly mice were used as model,aly/+mice as NIK normal control;cell populations were determined by FACS and the thymus structure were analyzed by immunohistochemistry.Results:The CD4+CD25+CD8- population were remarkably decreased in aly mice;and the UEA-1 positive cells were absent in aly mice.Conclusion:The autoimmune disease in aly mice might be the result of deceased the CD4+CD25+ population;the UEA-1 positive cells might play an important role in the development of CD4+CD25+ population. [

Objective To investigate the effects of angiotensin Ⅰ-converting enzyme inhibitor Enalaprilat(Ena) on proliferation and nitric oxide synthase-nitric oxide system of neonatal rat cardiac fibroblasts(CFb)and to probe its anticardiac fibrosis mechanism.Methods The cultured neonatal Wistar rat CFb were divided into control group,model group and three doses of Ena groups.CFb were isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,flow cytometry was used to measure cell cycle,nitric acid reductase method and spectrophotometry were used to detect the NO contents and NOS activity respectively with Ena.Results Ena could inhibit CFb proliferation induced by AngⅡ,there were significant differences of MTT values between model group and Ena groups after treated for 24,48 and 72 h(P

Objective To study the protective mechanism of Gross Saponin Tribulus Terrestris(GSTT) on the neonatal rat ventricular cardiocytes injured by hypoxia and the effect on protein kinase C(PKC).Methods The hypoxia-ischemia model was performed by treating the cultured neonatal rat ventricular cardiocytes with NaCN.The effect of GSTT(100 and 30 mg?L~(-1)) on the contents of ?PKC and ?PKC were detected by flow cytometry and laser confocal microscopy system.Results GSTT up-regulated ?PKC and ?PKC expressions.The contents of ?PKC and ?PKC in GSTT 100 mg?L~(-1) group(1 325.00?53.25,810.55?36.89;66.22?6.23,40.12?2.21) were increased significantly than those in model group(792.00?32.36,492.40?30.15;32.70?2.78,29.28?4.82)(P

Aim To investigate the effects of angiotensinI-converting enzyme inhibitor enalaprilat on proliferation,Endothelin-1 and Nitric oxide System of neonatal cardiac fibroblasts and to probe its mechanism of inhibiting cardiac fibrosis.Methods CFb was isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,HYP method was used to test the collagen synthesis;FCM for cell cycle;Nitric acid reductase method,radio-immunological method was used to detect NO content and ET-1 activity respectively.Results Ena decreased contents of MTT and HYP dramatically.The percentage ratio of phase G0/G1 was increaseded and the percentage radio of phase S was decreased.NO content was increased and ET-1 activity was dropped in a dose and time-dependent manner.Conclusion The anti-proliferative effects of Ena on CFb can be attributed to dropping endothelin-1 activity and enhancing Nitric oxide.

Objective:To investigate the surgical treatment for compression of inferior vena cava (IVC) by hepatic hydatid cyst.Methods:The retrospective and descriptive study was conducted. The clinical data of 10 patients with hepatic hydatid cyst compressing inferior vena cava who were admitted to the First Affiliated Hospital of School of Medicine of Shihezi University from May 2013 to May 2018 were collected. There were 7 males and 3 females, aged (46±5)years, with a range from 38 to 51 years. Patients underwent subadventitial close total pericystectomy, subadven-titial partial pericystectomy or subadventitial close total pericystectomy with partial IVC resection according to the relative distance between hepatic hydatid cyst and IVC, and the feasibility of total pericystectomy. Observation indicators: (1) surgical situations; (2) postoperative situations; (3) follow-up. Follow-up was conducted using outpatient examination or telephone interview. Patients were followed up at the outpatient department at postoperative 3, 6, 12, 24 months using color Doppler ultrasound examination to detect the recurrence of hepatic hydatid cyst and inferior vena cava occlusion. The endpoint of follow-up was 2 years after operation, and the secondary endpoint of follow-up was recurrence of hepatic hydatid cyst. The follow-up time was up to May 2020. Measure-ment data with normal distribution were represent as Mean±SD, and count data were described as absolute numbers. Results:(1) Surgical situations: 10 patients underwent surgery successfully without massive hemorrhage and septic shock during the operation, including 7 patients undergoing subadventitial close total pericystectomy, 2 patients undergoing subadventitial partial pericys-tectomy, and 1 patient undergoing subadventitial close total pericystectomy with partial IVC resection. The operation time and volume of intraoperative blood loss of 10 patients were (99±27)minutes and (99±48)mL, respectively. (2) Postoperative situations: the time to drainage tube removal and duration of postoperative hospital stay of 10 patients were 2-5 days and (7.1±1.8)days. None of patients underwent postoperative bleeding, residual cavity infection, biliary fistula, or perioperative death. (3) Follow-up: of 10 patients, 9 patients completed the endpoint of follow-up and no recurrence of hepatic hydatid cyst or inferior vena cava occlusion formation was detected. The other 1 patient was followed up at postoperative 3, 6 months but failed to be followed up at postoperative 12 months, and no recurrence of hepatic hydatid cyst or inferior vena cava occlusion formation was detected during the follow-up time.Conclusion:Complete resection of hepatic hydatid cyst can be achieved by subadventitial close total pericystectomy, subadventitial partial pericystectomy or subadventitial close total pericystectomy with partial IVC resection.

Objective Acute respiratory dysfunction (ARD) can occur after aortic surgery with the use of cardiopulmonary bypass and deep hypothermic circulation arrest, but relatively little is known about acute respiratory dysfunction in the patients with type A aortic dissection. This study aims to analyze the independent risk factors of acute respiratory dysfunction after A type aortic dissection surgery and to assess possible prevention and treatment option in the future. Methods Clinical data of the 252 patients including 193 male patients and 59 female patients who underwent type A aortic dissection surgery from February 2009 to October 2010 were collected. The mean age was 47 years. Postoperative acute respiratory dysfunction was defined as oxygenation impairment (PaO2/FiO2 ＜ 150) that occurred within 72 h of surgery except pleural effusion, cardiogenic pulmonary edema, pneumonia, pulmonary embolism and haemato-/ pneumothorax. There were 187 acute A type aortic dissection patients and 65 chronic type A aortic dissection patients. Clinical characteristics including age, gender, weight, height, history of hypertension, history of smoking, preoperative complications such as preoperative shock and acute renal failure, pericardial effusion, previous cardiac surgery, time from event to surgery, malperfusion syndrome, cardiopulmonary time, cross-clamp time,deep hypothermia circulation arrest time, surgical procedure, duration of intensive care unit stay and postoperative complications including tracheotomy, dialysis dependent renal failure and hospital mortality were gathered. Arterial blood analysis, chest X ray, ventilator parameters, number of blood transfusion and flood balance were assayed after operation. All the factors were evaluated by means of univariate and multivariate logistic regression analysis to identify relative risk factors of ARD. Results Acute respiratory dysfunction occurred in 32 (12.7% ) patients. The in-hospital mortality was significant difference between acute respiratory dysfunction group and non- acute respiratory dysfunction group (P ＜ 0.05). The value of BMI, incidence of acute aortic dissection, preoperative SBP level, cardio-pulmonary bypass time, aortic clamp time and total arch replacement in acute respiratory dysfunction group were significantly higher than the values in non- acute respiratory dysfunction group. Multivariate Logistic regression analysis showed blood transfusion more than 10 units and cardio-pulmonary bypass time more than 160 minutes were independent risk factors of early stage acute respiratory dysfunction after type A aortic dissection surgery.Conclusion Acute respiratory dysfunction after type A aortic dissection was a severe early stage postoperative complication and was associated with in-hospital mortality. The patients in acute aortic dissection were prone to have acute respiratory dysfunction. The independent risk factors of acute respiratory dysfunction included blood transfusion more than 10 units and cardio-pulmonary bypass time more than 160 minutes.