Objective To research the improvement effect of acupuncture combined with massage on clinical symptoms and function of cervical vertebra in patients with vertebral artery type of cervical spondylosis.Methods 100 patients with vertebral artery type of cervical spondylosis were included in this study.They were divided into the observation group (52 cases) and the control group(48 cases) by the random number table method.The patients in the control group were treated with conventional treatment,and the observation group was given acupuncture combined with massage on the basis of the conventional treatment.The clinical efficacy,the improve degree of clinical symptoms and cervical vertebra function were compared between the two groups.Results The total effective rate of the observation group was 94.23 ％,which was significantly higher than 79.17 ％ of the control group,and there was statistically significant difference between the two groups (x2 =5.008,P =0.025).Before treatment,there were no significant difference between the two groups in terms of symptom scores and hemodynamic parameters (all P ＞ 0.05).After treatment,the clinical symptoms scores of the two groups were significantly higher.The average blood flow velocity (TMFV),peak systolic velocity(PSV),pulsatility index(PI) and resistance index(RI) of the observation group were (26.87 ±5.14) cm/s,(61.63 ± 10.11) cm/s,(0.74 ± 0.13),(0.53 ± 0.08),respectively,which of the control group were (24.18 ±4.48)cm/s,(54.47 ±9.34)cm/s,(0.92 ±0.22),(0.66 ±0.13),respectively,there were statistically significant differences between the two groups (t =2.779,3.669,5.026,6.073,P =0.006,0.000,0.000,0.000).Conclusion Acupuncture combined with massage in the treatment of vertebral artery type of cervical spondylosis,the local blood flow of patients is significantly increased,and the blood supply of the brain is effectively alleviated,so as to improve the clinical symptoms and cervical function.It can play a positive role in the treatment of vertebral artery type of cervical spondylosis.

[Objective] To observe the effect of Puji Xiaodu Decoction (PXD) on immune function in mice. [Methods] Thirty NIH mice were randomized into three groups. Group A was treated with normal saline, group B with 2.9 g/kg PXD and group C with 11.6 g/kg PXD for 7 days. After treatment, interleukin 2 (IL-2) production and natural killer cell (NK) activity in splenocytes and lymphocyte proliferation in the spleen were detected. [Results] PXD increased the NK activity and IL-2 production, and promoted the proliferation of splenic lymphocyte, the difference being significant as compared with group A ( P

【Objective】To compare aristolochic acid(AA)content in Caulis Aristolochiae Manshuriensis(CAM)and in the separated prescriptions of Longdan Xiegan Decoction(LXD),and to explore the effect of Chinese herbal medicine compatibility on AA content.【Methods】According to the herbs percentage compared to LXD,the herbs samples were named after: CAM group,LXD group,CAM group with heat-clearing herbs,CAM group with yin-nourishing herbs,CAM group with herbs for promoting urination,CAM group with Radix Glycyrrhizae,and LXD group with CAM removed.The AA content was determined by reverse phase HPLC.The chromatographic conditions were as follows: C18 Diamonsiltm analytical column,gradient eluted with a mixture of methyl alcohol,water and acetic acid(volume proportion as 74:24:1),and detection wavelength at 315 nm.【Results】AA content was the lowest in LXD group,and was lower in CAM group with yin-nourishing herbs than that in CAM group.【Conclusion】Subjecting to the theory of Chinese herbal medicine compatibility,it is feasible to reduce the CAM toxicity by composing a prescription with proper proportion of herbs.

Objective To explore the therapeutic mechanism of Jiaotai Pill(JP) for restoring normal coordination between the heart and the kidney.Methods Fifty SD rats were equally randomized into normal group,model group,Huanglian(Rhizoma Coptidis,15g) group,Rougui(Cortex Cinnamomi,1.5g) group,and JP group(the ratio of Huanglian and Ruigui being 15∶1.5).Insomnia rat models were induced by intraperitoneal injection of one-dose para-chloroamphetamine acid(PCPA,300mg/kg),and the serum interleukin-1(IL-1) and tumor necrosis factor(TNF-?) levels were detected by enzyme-linked immunosorbent assay(ELISA).Results In the model group,the serum levels of IL-1 and TNF-? were decreased(P0.05 compared with the model group),and increased in JP group(P

In recent years, researchers have shown great interest in the mechanism of induced programmed cell death (PCD) in tumor cells. Pyroptosis is a newly-discovered programmed cell death, which has a proinflammatory effect and is accompanied by cell pore formation, cytoplasmic swelling, cell membrane rupture, and release of cytoplasmic contents into the extracellular environment. A large number of studies have shown that pyroptosis is closely related to the occurrence and development of various diseases such as cancer. Currently, gasdermin family mediate cell pyroptosis, due to gasdermin D and gasdermin E are two molecules that are extensively studied in pyroptosis. This article mainly explores the relevant mechanism of these two proteins and their relationship with cancer, which will broaden our understanding of new insights to cancer and provide a new perspective on cancer prevention and treatment.

Objective To evaluate the significance of molecular microenvironment in neurons for the nerve regeneration and repair by investigating the dynamic changes of nerve regrowth-associated proteins following bilateral sciatic nerves crush in pyridoxine-induced ganglionopathy rats model.Methods Bilateral sciatic nerve crush were performed 4 weeks after induction of pyridoxine-induced ganglionopathy. The changes of mean percentage of TUNEL positive cells in dorsal root ganglion (DRG) following bilateral sciatic nerves crush for 7 days, 14 days, 21 days, and 28 days. Western blotting techniques were used to investigate the expression of GAP-43 and trk A in different duration following sciatic nerve crush injury.Results The percentage of TUNEL positive neurons in DRG significantly increased in early stage and markedly decreased in 21～28 days after sciatic nerve crush. The expression of GAP-43 and trk A in DRG were upregulated at all time point after nerve injury in pyridoxine-induced ganglionopathy, but the overall level was lower than that of pure nerve crush injury.Conclusion In pyridoxine-induced ganglionopathy, neurons in DRG undergo survival crisis, the gene expression system was disintegrated, the capacity to regenerate their axons declines after nerve injury.

【Objective】To observe the effect of Ma Xing Shi Gan Decoction(MXSGD)on metabolism of oxygen free radicals in mice with bronchial asthma.【Methods】Forty BALB/c mice were randomized into 4 groups:normal group,model group,low-dose MXSGD group(20?g?kg~(-1)?d~(-1))and high-dose MXSGD group (80?g?kg~(-1)?d~(-1)).Except the normal group,the mice in other 3 groups received sensitization by intraperitoneal injection of ovalbumin(OVA)and challenge by inhalation of OVA to induce bronchial asthma.Meanwhile,MXSGD groups were given the corresponding doses according to the experimental design by gastric gavage,and normal group and model group received the same volume of normal saline for 27 days.Twelve-four hours after last challenge,blood sample was taken and homogenate of lung tissue was prepared for the detection of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)contents in the plasma and the lung tissue.【Results】High-and low-dose MXSGD increased SOD and GSH-Px contents and decrease MDA content in the lung tissue and plasma(P

AIM:To determine the effects of Tongxinluo (TXL) on connexin 43 (Cx43) remodeling and ven-tricular arrhythmia ( VA) after myocardial infarction ( MI) in rats .METHODS:Male SD rats were randomly divided into sham-operated (sham) group (n=25) and operation group (n=75).The left anterior descending (LAD) was ligated in operated group , while the rats in sham group only underwent pericardiotomy .The rats in operation group which survived for 3 d after operation were randomly assigned to TXL group and MI group .The rats in TXL group was administrated with TXL (2 g? kg-1? d-1, intragastric administration) for 4 weeks, while normal saline was applied to the rats in sham group and MI group.The levels of interleukin-1β(IL-1β) and endothelin-1 (ET-1) in the tissue from the border zone were measured by ELISA after treatment .The distribution and the mRNA and protein expression of Cx 43 were detected by immunohisto-chemical staining , RT-PCR and Western blotting , respectively .The burst pacing was used to induce ventricular arrhythmia ( VA) .RESULTS:Compared with sham group , the levels of IL-1βand ET-1 and the incidence of VA were significantly increased , while the mRNA and protein expression of Cx 43 was markedly reduced with irregular distribution in MI group (P<0.05).Compared with MI group, the levels of IL-1βand ET-1 and the incidence of VA were significantly reduced , while the expression of Cx 43 at mRNA and protein levels was markedly increased with augmented linear distribution in the myocardial cell intercalated disc in TXL group (P<0.05).CONCLUSION:TXL reduces the incidence of VA after MI via inhibiting the Cx43 remodeling .

0.05), but LA patients had less postoperative pain,less time to ambulation after operation, less time to food intake after operation, less average length of hospital stay,less rate of incision infection and less intestinal adhesion than OA did(all P

Objective To systematically review the efficacy and safety of mini-percutaneous nephrolithotomy (MPCNL) and flexible ureteroscopic lithotripsy (FURL) in management of lower pole kidney stones.Methods PubMed, Embase, Scopus, Cochrane library, CNKI, VIP and Wanfang Data were searched from the beginning of database to September 2016 and clinical trials that compared the two above operation for treatment of lower pole stones were collected.Two researchers independently filtered literature,extracted data and evaluated the methodological quality of research papers.The meta-analysis was performed using the RevMan 5.3 software.Results One randomized and seven non-randomized studies were analyzed, which consists of 621 patients including MPCNL group 327 cases, FURL group 294 cases.The results of meta-analysis showed that MPCNL was better than FURL in stones clearance (OR =2.65,95％ CI 1.58-4.46,P ＜ 0.01) and operative time (WMD =-21.86,95％ CI-28.52--15.20, P ＜ 0.01).FURL was better in hospital stay time (WMD =2.28,95％ CI 0.29-4.28, P =0.02), decrease in haemoglobin levels (WMD =0.78,95 ％ CI 0.68-0.89, P ＜ 0.01), bleeding (OR =5.11,95 ％ CI 1.12-23.31,P =0.04), transfusion(OR =7.04,95％ CI 1.59-31.15, P =0.01).There was no significant difference in fever,urinary tract infection, hematuria (P ＞ 0.05).Conclusions Both MPCN and FURL are safe and effective for the treatment of lower pole stones, MPCNL can get higher stone clearance rates and shorter operation time.However, FURL can get shorter hospitalization time and lower complication rates.