Sample preparation and separation is a very crucial and complicated process in proteomics research.Based on the actual condition of university and students,this article explored the way to improve the teaching quality of sample preparation and separation in proteomics course for medical post-graduates aiming at cultivating and enhancing the students' scientific thought,making them have a better understanding of the rules,technologies,strategies in sample preparation and separation procedure.

INTRODUCTIONThere has been an explosive growth of Internet usage worldwide and this is expected to continue with its use becoming an integral part of everyday life. The Internet provides tremendous educational benefits; however, excessive Internet use can lead to negative outcomes such as poor school performance and social isolation.

MATERIALS AND METHODSThe survey consisted of a 69-item, anonymous, self-administered paper-and-pencil questionnaire. Data collected included demographic data, academic performance, social support and general wellbeing as well as questions pertaining to Internet use. For the purposes of our study, we defined Internet use of more than 5 hours a day as "excessive use".

RESULTSOf the 2735 adolescents who took part in the study, 1349 (49.3%) were male and 1383 (50.6%) were female. The mean age of the adolescents was 13.9 years [standard deviation (SD), 1.0]. A quarter of the adolescents surveyed (25%) reported that they did not access the Internet everyday, while 17.1% of adolescents reported using it for more than 5 hours every day. Excessive Internet use was associated with (i) no rules of Internet use at home (x2 = 313.1, P <0.001 ), (ii) less likelihood of having confidants (x2 = 15.8, P = 0.003), (iii) feelings of sadness or depression (x2 = 49.6, P <0.001) and (iv) perceived poorer grade/school work (x2 = 226.1, P <0.001).

CONCLUSIONSThe high figures of excessive Internet use (17.1%) reported in our study is not equivalent to Internet addiction as no diagnostic instruments were used. However, school counsellors and teachers need to be made aware of the prevalence of and problematic behaviours associated with excessive Internet use. Training and resources should also be made available to parents and caregivers so that they can play a greater role in setting boundaries and detecting early warning signs.

Adolescent ; Adolescent Behavior ; Behavior, Addictive ; Data Collection ; Female ; Humans ; Internet ; utilization ; Male ; Singapore ; Social Isolation

INTRODUCTIONPathological gambling is defined as the failure to resist the impulse to gamble despite disruption to personal, family and vocational life. It appears to be highly comorbid with psychiatric disorders. This study aims to describe the socio-demographic profile and comorbidity of 40 pathological gamblers compared to controls.

MATERIALS AND METHODSQuestionnaires were administered to 40 cases (90% male, mean age = 38.3, SD = 10.0) scoring 5 or more in the South Oaks Gambling Screen (SOGS) and verified to be pathological gamblers based on DSM-IV criteria and 40 age-and-gender-matched controls.

RESULTSStudy results showed that the prevalence of lifetime DSM-IV diagnoses were significantly different between cases and controls (chi(2) = 16.4, P <0.001), with 67.5% of the cases and 22.5% of the controls meeting the criteria, respectively. Eighty percent of the cases had at least one personality disorder compared to 45% in the control (chi(2) = 10.5, P <0.005). The common lifetime disorders observed in the cases were substance abuse disorder and anxiety while common personality disorders were obsessive-compulsive, avoidant and paranoid.

CONCLUSIONThis study highlights the high rates of comorbidity in a largely Chinese sample in an Asian setting and the importance of assessing pathological gamblers for comorbidities.

Adult ; Asia ; ethnology ; Comorbidity ; Demography ; Female ; Gambling ; psychology ; Humans ; Male ; Mental Disorders ; epidemiology ; ethnology ; Middle Aged

INTRODUCTIONFew studies have examined the impact of pathological gambling on quality of life especially in the Asian context. The aim of the current study was to examine the quality of life in pathological gamblers in a multiracial population in Singapore and we hypothesised that those with pathological gambling would have poorer quality of life as compared to controls.

MATERIALS AND METHODSForty subjects with "compulsive gambling behaviour" were recruited and matched (for gender and age) with 40 controls. Subjects with pathological gambling were compared with control subjects with regard to sociodemographic data as well as on the World Health Organization Quality of Life assessment - abbreviated version (WHOQOL-BREF).

RESULTSA one-way MANOVA revealed that pathological gamblers had significantly diminished quality of life as compared with the healthy controls using the summary scores of the 4 domains of quality of life (Pillai's Trace = 0.338, F = 9.5, P <0.001). Univariate tests indicated subjects with pathological gambling scored significantly lower on physical health, psychological, social relationships and environment domains of quality of life compared with subjects without pathological gambling.

CONCLUSIONOur study found that those with pathological gambling had lower scores than the controls in all the domains of the quality of life scale. The impact and the extent of pathological gambling on the quality of life should be borne in mind -- not only as a consideration in the management but also as an important indicator of treatment outcome of pathological gamblers.

Adult ; Analysis of Variance ; Asian Continental Ancestry Group ; statistics & numerical data ; Case-Control Studies ; Competitive Behavior ; Confidence Intervals ; Cross-Sectional Studies ; Ethnic Groups ; statistics & numerical data ; Female ; Gambling ; psychology ; Health Surveys ; Humans ; Male ; Psychometrics ; Quality of Life ; psychology ; Singapore ; ethnology ; Surveys and Questionnaires ; Young Adult

Objective To establish a HPLC method for the simultaneous determination of artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L.Methods The analysis was performed on Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column with a mobile phase of acetonitrile(A)-water(B)and the flow rate of 0.8 mL·min-1 in a gradient elution manner.The column temperature was 30℃.The injection volume was 10 μL,and the detection wavelength was 210 nm.Results Artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D were correlated well linearly with peak area in their respective ranges 1.608 8-16.088 μg(r=0.999 9),0.014 1-0.141 4 μg(r=1),0.185 1-1.850 9 μg(r=0.999 9),0.144 1-1.441 4 μg(r=0.999 9),the average recovery rate(n=6)were 102.44%,97.82%,95.07%,95.55%,and the RSD values were 1.12%,1.44%,1.29%,1.53%.Conclusion This method is convenient and accurate.It has good stability and repeatability,and can be used to simultaneously determine the content of artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L.