Objective To discuss digital CR mammography and common screen/film radiography.Methods 200 cases of digital CR mammography were compared with those of common screen/film radiography.Results Digital CR mammography had more diagnostic value than common screen/film radiography.Conclusion Digital CR mammography can demonstrate the lesion of mammary gland tumor,such as tiny calcification,barb sign and small nodules.

Objective To evaluate the effect of the radiography in prediction of correction of scoliosis cooperated with clinical treatment.Methods 40 case of adolescent idiopathic scoliosis(AIS) with 45 structural curves were studied,preoperative X-films of standing,supine anterior-posterior and lateral plane,supine anterior-operation in thoracic and fulcrum-bending.Results The mean Cobb angle of pre-operation in thoracic and lumber curves were 43?and 22?,38?and 19?in fulcrum bending,37?and 19?in post-operation.In severe and moderate group the average Cobb angle were 52? and 22? in supine side-bending,49? and 19? in fulcrum bending,45? and 22? in post-operation.Conclusion The fulcrum-bending X-films can offer more information for clinic.

Objective To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on autophagy and the secretion of chemokine receptor CXCR4 induced by low-dose immunosuppressive durgs.Methods Flow cytometry was used to detect the changes of hUC-MSCs surface markers after treatment with low-dose tacrolimus and rapamycin.The effect of treatment with tacrolimus and rapamycin on proliferation of hUC-MSCs was analyzed with WST-1 assay.Regular RT-PCR was applied to analyze the mRNAs expression of ligands such as LC3B,Atg5 and Beclin1 in hUC-MSCs.Western blotting was carried out to detect the expression of LC3B,Atg5,Beclin1 and p-ULK1 in hUC-MSCs after treatment with tacrolimus and rapamycin.The secretion of chemokine receptor CXCR4 in hUC-MSCs was analyzed under the state of autophay by flow cytometry.Results Flow cytometry analysis confirmed low-dose immunosuppressive drugs tacrolimus and rapamycin did not cause changes in hUC-MSCs phenotypes significantly.Low-dose tacrolimus had no cytotoxic effect on hUC-MSCs,while,rapamycin could inhibit the proliferation of hUC-MSCs after 24 h or 48 h,with survival rate being 73.66％ and 68.81％ (P＜0.05) of controls,respectively.Moreover,both tacrolimus and rapamycin could inhibit PI3K/AKt/mTOR signaling pathway to activate hUC-MSCs autophagy,and the related proteins of LC3B,Atg5 and Beclin1 increased significantly and induced the up-regulation of CXCR4 secretion.Conclusion Our results here demonstrated that low-dose tacrolimus and rapamycin induce autophagy in hUC-MSCs and promote the secretion of CXCR4.