Objective： To investigate pathophysiologic mechanism about initiation and propagation of epilepsy in a genetically epilepsy-prone rat (GEPR) model. Methods： The hippocampal MF sprouting of P77PMC rats was examined at different stages in the course of recurrent seizures using Timm method of sulfide silver staining. Results：The three groups of P77PMC rats which experienced 30 and less than 30 times of audiogenic seizure ( AGS ) and the Wistar control group did not display MF sprouting in dentate gyrus, however, the group of P77PMC rats which experienced 50 times of AGS was found hippocampal MF sprouting into the inner molecular layer of dentate gyrus. Conclusion：Our findings suggest that hippocampal MF sprouting presented not only in limbic seizure, but also in AGS, the seizure initiated in brainstem but rapidly generalized; in AGS-prone rats, recurrent AGSs can induce MF synaptic reorganization in hippocampus.

Objective To observe the changes and its significance of the levels of serum cytokines——interleukin-(IL)1?,IL-6,tumor necrosis factor(TNF)-? and C-reactive protein(CRP) in patients with cerebral infarction(CI).Methods The serum IL-1?,IL-6 and TNF-? level were detected by radioimmunnoassay and serum CRP level by immunoturbidimetry in 30 CI patients with DM(DMCI group),30 CI patients without DM(NDMCI group),20 patients with DM(DM group) and 20 normol controls(NC group).The situation of CI patients were assessed by modified Edinburgh-Scandinavian stroke scale(ESSS).Results The serum levels of IL-1? [(0.60?0.04)ng/ml,(0.33?0.03)ng/ml,(0.30?0.02)ng/ml],IL-6 [(231.07?7.68)pg/ml,(141.34?6.50)pg/ml,(118.92?5.82) pg/ml],TNF-?[(2.70?0.11)ng/ml,(1.85?0.11)ng/ml,(1.21?0.13) ng/ml] and CRP [(7.44?0.26),g/L,(4.67?0.21)mg/L,(4.54?0.24)mg/L] in DMCI group,NDMCI group and DM group were significantly higher than those in NC group [(0.20?0.03)ng/ml,(60.99?5.98)pg/ml,(0.70?0.10) ng/ml,(3.83?0.14) mg/L](all P

Resveratrol synthase (RS) plays a key role in resveratrol (Res) biosynthesis. RS gene has been formerly reported to be transformed into many plant species and microorganisms, and to play certain roles in metabolic and regulation processes. In this paper, the transformations of RS gene in plants, and the related changes of biological properties, such as metabolites, anti-pathogen activities, anti-radical properties, and developmental characters in transgenic plants, as well as the production of resveratrol in microbes by utilizing RS gene were summarized. Moreover, the application prospects of RS gene in bioengineering were also addressed.
Acyltransferases ; genetics ; Genetic Engineering ; Plants, Genetically Modified ; enzymology ; genetics ; Stilbenes ; metabolism

Objective To investigate the effect of hydrogen sulfide (H 2 S or NaHS)on myo-cardial ischemia reperfusion injury induced in type 2 diabetic rats in vivo and the role of AMP-activated protein kinase (AMPK)signal pathway.Methods The induced type 2 diabetic rat models were anesthetized,left thoracotomy were performed.All the models were randomly divided into six groups (n = 14):group Sham;group IR:the left anterior descending artery was ligated 30 min, reperfused for 4 hours;group CC:prior to thoracotomy,compound c was intraperitoneally injected 250 μg/kg,then received the same treatment as group IR;group DMSO received the same treatment as compound c group but DMSO was injected intraperitoneally as control;group NaHS:the left ante-rior descending artery was injected NaHS 0.05 mg/kg then reperfused for 4 hours;group CC +NaHS:prior to thoracotomy,compound c was intraperitoneally injected 250 μg/kg,then NaHS 0.05 mg/kg injected intravenously and reperfused 4 hours.All the rat models euthanatized,infarcted area was detected by TTC assay.The AMPK,LC3 and p62 were analyzed by Western blot.Results Com-pared with group Sham,the infarcted area and concentration of AMPK,LC3 and p62 were increased in other groups (P <0.05).Compared with group IR,the infarcted area and concentration of LC3, p62 markablely decreased in group NaHS (P < 0.05 ).Compared with group NaHS,the infarcted area and concentration of LC3,p62 significantly increased but AMPK down-regulated in group CC+NaHS (P <0.05).Conclusion Hydrogen sulfide could alleviate myocardial infraction via AMPK sig-nal pathway in type 2 diabetic rats'IR models.

Huolinguole lignite was sequentially extracted with carbon disulfide, ethyl acetate, methanol and acetone.All of the extracts were analyzed using a time-of-flight mass spectrometry (TOF-MS) equipped with an atmospheric pressure photoionization (APPI) ion source.Toluene or 1,4-difluorobenzene was chosen as dopant for APPI.The results indicated that both dopants could well ionize compounds which could not be ionized by APPI without dopant.Toluene induced higher ionization efficiency than 1,4-difluorobenzene.Some compounds in the extracts were identified as dimers, which might be formed via molecular association.Heteroatoms were identified in all of the associated molecules.Molecular weight distributions under three APPI ionization modes were similar.Compounds with molecular weight from 200 to 500 Da occupied 60% of all the products and around 10% of the products had molecular weight over 500 Da.

Objective To characterize the clinical manifestations and to evaluate the value of the electrophysio-logical examination in patients with peripheral nerves injuries caused by neurobrucellosis. Methods Electrophysiology examination was conducted in 32 patients with peripheral nerve injuries caused by neurobrucellosis and 32 normal controls who had equivalent age and gender. The results were further statistically analyzed. Results There were sig-nificant difference between patients and healthy control group in distal motor latency (DML), compound motor active potentials (CMAP) amplitude, motor nerve conduction velocity (MCV), sensory nerve action potential latency(SL),senso-ry nerve action potential (SNAP) amplitude and sensory nerve conduction velocity (SCV) ( P﹤0.05). Electrophysiology examination revealed peripheral nerve damage of limbs involving, both sensory nerve and motor nerves, of which sen-sory and motor nerve injuries accounted for 55.47% and 16.80%, respectively. Median and sural nerve injuries were most frequently affected in upper (64) and lower limbs (16). Motor and sensory nerve conduction velocity was delayed in 43 nerves (16.80%) of 256 examined motor nerves and in 142 nerves (55.47%) of 256 examined sensory nerves. The damage of the sensory nerves was more severe than those of the motor nerves and damage of nerves in the upper limbs was more severe than those in the lower limbs. Conclusion Electrophysiologoical examination provides objective basis for the diagnosis of peripheral nerve injuries in neurobrucellosis.

24.34, the sensitivity and specificity were 95.0% and 75.8%, respectively. Conclusion: The autofluorescence spectrum of dilute gastric juice may become an effective means in the diagnosis and screening of gastric carcinoma.

Objective:To investigate the clinical characteristics of atypical neurobrucellosis.Methods:Retrospective analysis was made on the epidemiological characteristics, clinical manifestations, laboratory and imaging findings of five cases of atypical neurobrucellosis admitted to the People's Hospital of Inner Mongolia Autonomous Region from December 2020 to June 2021.Results:The age of the five cases ranged from four to sixty-nine years old, including three females and two males. Four cases had a clear history of sheep contact. Serum F1 antibody against brucellosis was positive in all five cases, serum tube agglutination test (SAT) was positive in three cases, lumbar puncture cerebrospinal fluid (CSF) examination showed increased intracranial pressure in four cases, and bacterial smear and tuberculosis culture were negative in all of the five cases. After magnetic resonance imaging (MRI), two cases were found to have abnormal intracranial high signals, of which one case was abnormal in bilateral frontal parietal lobe and right temporal occipital lobe, showing long T1 and T2 signals and increased fluid-attenuated inversion recovery sequence(FLAIR) signal; and another case was abnormal in bilateral corona radiata, posterior limbs of internal capsule and bilateral cerebral peduncles, it showed continuous T2 and FLAIR slightly high signal intensity. One case had abnormal signal in the spinal cord, showing a small patch like long T2 signal on the right side of the spinal cord at the level of C2-3 discs. Electromyography was abnormal in one case. Among the five cases, two cases presented with brucellosis encephalitis, one case with brucellosis myelitis, one case with glossopharyngeal nerve damage caused by brucellosis, and one case with brucellosis cerebrospinal neuropathy. All five patients were treated with a combination of doxycycline, rifampicin and ceftriaxone, and three patients had a good prognosis.Conclusions:The clinical manifestations of atypical neurobrucellosis are various. Clinicians should strengthen their understanding of the disease and reduce the chance of missed diagnosis and misdiagnosis.

Objective The clinical characteristics of central nervous system neurobrucellosis (NB),such as incidence features,clinical symptoms,laboratory tests and imaging manifestations,are summarized to provide referenced clinical data for improving diagnosis standard.Methods Thirty-two in-patients with central nervous system NB were chosen in our hospital from March 2011 to December 2014.The clinical data were collected after they underwent some supplementary examinations,and then,follow-up was performed after 6 months of treatment.Results (1) The proportion of male and female was 5.5:1,and the average age was 50 years.(2) Twenty-four patients had onset with infectious of the central nervous system;another 8 had onset with cerebrovascular disease.(3) The clinical symptoms included fever,headache,nausea and vomiting and limb weakness;the signs included pathological syndrome,decreased muscle strength,and meningeal irritation sign.(4) All 32 NB patients underwent lumbar puncture,including 15 with normal cerebrospinal fluid pressure,and routine and biochemical examinations,4 with atypical NB cerebrospinal fluid changes,5 with NB early changes,and 6 with NB late changes.(5) Twenty-three patients appeared with positive imaging performance;15 patients were noted having lesions in the cortex,semi oval center,paraventricular and basal ganglia,3 in the spinal canal,one in the cervical spinal cord,two in the thoracic spinal cord,one in the cerebellar hemisphere and one in the dura mater.(6) After systematical treatment,one died,the other 31 recovered,of which 9 had different degrees of sequela.Conclusions NB is a part of the whole of Brucella infection.Its nonspecific clinical manifestations and extensive clinical syndrome bring some difficulties to definite diagnosis.Cerebrospinal fluid and neuroimaging examination are important evidences for the diagnosis.So it is necessary to study and understand these characteristics deeply to provide valuable data for early diagnosis and treatment of the disease.

Objective To analyze the clinical characteristics and prognosis of neurobrucellosis(NB).Methods Twenty-seven cases of NB patients who received treatment in Disease Prevention and Control Center of Inner Mongolia Autonomous Region and Inner Mongolia Medical University Hospital from January to December in 2016 were collected.The clinical data of these patients were recorded and systematically analyzed.Results Twenty-four cases(88.89%)had a history of exposure to cattle and sheep.Twenty-four cases(88.89%)were admitted with fever,18(66.67%)cases with sweat,16 cases(59.26%)with headache,14 cases(51.85%)with neurological symptoms of meningitis and meningoencephalitis,4 cases(14.81%)with auditory nerve damage and 3 cases(11.11%)with spinal cord damage.Elevated white blood cells were found in 2 cases(7.41%),elevated serum C-reactive protein in 3 cases(11.11%),elevated procalcitonin in 2 cases(7.41%)and elevated erythrocyte sedimentation rate in 15 cases(55.56%).All the 27 patients underwent lumbar puncture and the cerebrospinal fluid test results were abnormal,of which increased protein levels in 17 cases(62.96%),increased mononuclear cell ratio 14 cases(51.85%),increased pressure in 14 cases(51.85%),and reduced chloride levels in 14 cases(51.85%),and reduced glucose levels in 6 cases(22.22%).Positive cerebrospinal fluid culture(CSF)was found in 1 case(3.70%).There were 8 cases(29.63%)with white matter damage,5 cases(18.52%)with meningeal enhancement,3 cases(11.11%)with spinal cord lesions,2 cases(7.41%)with cerebral edema and 2 cases(7.41%)with brain abscess.There were 10 cases(37.04%)with sensory nerve damage in the extremities,4 cases(14.81%)with auditory nerve damage and 2 cases(7.41%)with motor nerve damage.All patients were treated with regular anti-Berg's disease for 6 weeks and were followed up for 1 year(every 3 months)after the treatment.Nineteen patients(70.37%)were cured,7 patients(25.93%)developed sequelae and 1(3.70%)patient died.Conclusions The analysis suggests that NB have a variety of clinical characteristics.CSF examination,imaging examination and neuro electrophysiology detection have contribution to the diagnosis of the NB.