1.The pharmacodynamic research of baijin capsule on depression in chronic unpredictable mild stress rats
Rong ZHANG ; Shifen DONG ; Boran NI ; Zhiqing WANG ; Jian NI ; Jianning SUN
Chinese Journal of Comparative Medicine 2015;(7):46-52
Objective To evaluate the effects of baijin capsule on behavioral changes and monoamine neurotransmitters concentration in chronic unpredictable mild stress ( CUMS ) depression rat model.Methods The depression rat model was induced by11-week chronic unpredictable mild stress combining with solitary.After the model were established, rats were given the decoction of baijin capsule ( 12.6 g/kg, 4.2 g/kg, 1.4 g/kg ) or fluoxetine hydrochloride (3.5 mg/kg) by intragastricfor 4 weeks.During the experiment period, sucrose consumption and open-field experiment were conducted to monitor the behavior of rats, such as sucrose consumption percentage, horizontal motion, and vertical motion.At the end of the experiment, the levels of the monoamine neurotransmitters in the cerebral cortex and hippocampus were analyzed by method of high performance liquid chromatography-electrochemistry.Results Compared with the normal group, the weight, horizontal displacement distance, vertical movement times, and sucrose consumption percentage of rats in model group decreased significantly after stimulated with CUMS and solitary for 7 weeks (P<0.05,P<0.01).Compared with model group, consecutively administrated for 4 weeks, horizontal displacement distance, vertical movement times, and the percentage in sugar water consumption significantly increased with the treatment of baijin capsule (P<0.05, P<0.01).Meanwhile, the content of norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamine (5-HT) in the cortex were significantly increased in rats of the baijin capsule ( P <0.05 ) .Conclusions The results indicated that baijin capsule improved the behavioral disturbances in depression rat model, which were related to enhancement of the concentration of monoamine neurotransmitters in the cortex.
2.Effect ofCoreopsis tinctoria Nutt. from Different Extract Regions on Proliferation and Differentiation of 3T3-L1 Preadipocytes
Mingxian JIA ; Yuan ZHANG ; Shifen DONG ; Dai DAI ; Jie YU ; Wenjie GU ; Zhiyuan LI ; Jing WANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;17(3):544-549
The 3T3-L1 preadipocytes were used as carriers in the investigation of total extract, n-butanol extract, CB-1 and CB-2 of Coreopsis tinctoria Nutt. on cell proliferation and differentiation. Three groups at different doses were set for each of the four extract regions of C. tinctoria Nutt., respectively. MTT assay was used to detect 3T3-L1cell proliferation by four extract regions of C. tinctoria Nutt. Oil Red O staining was used to analyze the formation and accumulation of cytoplasmic lipid during cell differentiation. The results showed that compared with the control group, there were significant inhibition on cell proliferation when thetotal extract of C. tinctoriaNutt. at 100 μg·mL-1, n-butanol extract at 0.5, 5, and 50 μg·mL-1, CB-1 and CB-2 at 50 μg·mL-1 (P< 0.01). N-butanol extract showed certain dose-dependent manner (r = -0.903). Oil Red O staining showed that compared with the control group, thetotal extract of C. tinctoria Nutt. at 1, 10, 100 μg·mL-1 can obviously inhibit cell differentiation, reduce the formation of cytoplasmic lipid (P< 0.01). N-butanol extract can inhibit cell differentiation in a dose-dependent manner (r= -0.779). CB-1 and CB-2 obviously inhibited cell differentiation at the concentration of 50 μg·mL-1 (P < 0.01). It was concluded that thetotal extract, n-butanol extract, CB-1 and CB-2 of C. tinctoria Nutt. can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes and reduce the formation of cytoplasmic lipid.
3.A Synchronus Study on the Iron Status of Mothers and Babies
ruwen, WANG ; xueyu, ZHANG ; zuzhao, ZHOU ; liyang, LI ; shifen, HAO ; yajun, XIE
Journal of Applied Clinical Pediatrics 1986;0(02):-
From August, 1984 to February, 1985, We kept a synchronous observation of 92mothers and their babies on the relationship of iron status between them. The 92 pregnantwomen, of whom the gestational periods were not longer than 36 weeks, were divided rando-mly into wo groups: a medication group and a control group. For all, Hb, Hct, FEPand SF were tested. In the 4th week after labor, Hb, Hct and SF of the mothers ofthe medication group were higher than that of the control group (P
4. Analysis of occupational stress and occupational exhaustion of primary caregivers
Shuhang XU ; Honger TIAN ; Liping ZHOU ; Yang WANG ; Li ZHANG ; Shifen JIA ; Lu LIU ; Xiaobo LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2019;37(3):197-201
Objective:
To investigate the occupation stress and job burnout of orphan child care workers in Nanjing and nursing staff in Yangzhong primary hospital, to compare the differences of occupational stress and job burnout between the two social service workers.
Methods:
From February to May 2017, a cross- sectional survey was conducted. Cluster sampling method was used to investigate the occupation stress and job burnout of orphan child care workers in Nanjing and nursing staff in Yangzhong primary hospital, totally 403 people, by a combination of the Internet of things assessment system of occupational stress and traditional questionnaire.
Results:
There were statistically significant differences of occupational stress and job burnout scores of both social service workers (
5. The influence of demographic characteristics on occupational stress of orphan child care workers
Li ZHANG ; Honger TIAN ; Liping ZHOU ; Yang WANG ; Shuhang XU ; Shifen JIA ; Liu LIU ; Xiaobo LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(12):908-911
Objective:
To investigate the occupation stress of orphan child care workers in Nanjing, to explore the influence of different demographic characteristics on occupational stress of orphan child care workers and propose measures.
Methods:
Cluster sampling method was used to investigate the occupation stress of 236 people of orphan child care workers in Nanjing by a combination of the Internet of things assessment system of occupational stress and traditional questionnaire.
Results:
The scores of workload, job prospects, participation decision-making, anxiety and logical in different age groups among orphan child care workers were statistically different (
6.Advances on myeloid-derived suppressor cells in hepatitis B virus infection
Shifen WANG ; Ni YAO ; Yan LYU ; Yuhong ZHAO ; Dawei CUI ; Jue XIE
Chinese Journal of Clinical Infectious Diseases 2023;16(3):234-240
Myeloid-derived suppressor cells (MDSCs) are a group of immature and heterogeneous cells that can inhibit T cell function. In pathological conditions such as tumors, infections, and chronic inflammation, the large expansion of MDSCs is involved in processes of immune escape, immune tolerance and inflammatory reactions. MDSCs are also crucial in the pathophysiology of hepatitis B virus (HBV) infection, however, their activation, differentiation, and function during HBV infection are still unclear. This article reviews the general characteristics and roles of MDSCs in HBV infection, as well as related drug therapies, in order to provide information for further research on the related mechanism and potential targeted treatment.