1.Study on Effects of Shenkang Oral Liquid on Resistant Senility
Shifang LI ; Zhirong WU ; Yunfen DONG ;
Chinese Traditional Patent Medicine 1992;0(08):-
Objective: To investigate the effects of clinical therapeutic and pharmacological actions “Shenkang Oral Liquid” on sexual function failing, senile nocturia. Results: Index of phagocyte, weight of seminal vesicle gland and content of testosterone in experimental rats with yang deficiency were observed by experiment.Results: “Shenkang Oral Liquid” could significantly raise index of phagocyte (k=0.0258?0.012), weight of seminal vesicle gland (24.07?1.99mg/10g) and content of testosterone (363?50.56pmol/mL) in rats with yang deficiency. Conclusion: “Shenkang Oral Liquid” shows enhancement effects on immune and sexual functions.
2.TNF-αenhances IFN-γ-induced NIT-1 cells apoptosis via death receptor pathway
Shifang DONG ; Guiqing LI ; Weifan JIANG ; Fei YANG ; Zhaohui CAO ; Guilian XU
Chinese Journal of Immunology 2014;(11):1448-1451
Objective:To investigate the effect of TNF-αand IFN-γon NIT-1 cells apoptosis and the apoptosis mechan-ism.Methods:NIT-1 cells were exposed to a combination of TNF-αand IFN-γtreatment.The viability of NIT-1 cells was assessed via MTT assay.The morphological changes of the cells and nuclei were observed under the inverted or confocal laser scanning microscope with Hoechst 33258 staining.The activation of Caspase-8,-3 and PARP was detected by Western blot.Results:TNF-αand IFN-γsig-nificantly inhibited NIT-1 cells viability, promoted cells apoptosis, induced the activation of Caspase-8,-3 and PARP cleavage.Conclusion:TNF-αcombined with IFN-γtreatment induced the apoptosis of NIT-1 cells through death receptor pathway.
3.Effect of Huanglian Wendantang on Inflammatory Response and Hepatocyte Burn Death in IGT Rats
Han LI ; Yun-lei HUANG ; Wan-ru DONG ; Bo-yan MA
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(8):25-32
Objective:To investigate whether there is inflammatory reaction and cell pyroptosis in impaired glucose tolerance (IGT) rats induced by high-fat diet and the intervention effect of Huanglian Wendantang. Method:Healthy male SD rats were fed with 45% fat content diet for 20 weeks to replicate the IGT model. The rats in line with the model establish criteria were randomly divided into 3 groups, with 10 rats in each group, another 10 rats were selected as the blank control group. Huanglian Wendantang group was given 7.8 g·kg-1·d-1 compound decoction of Huanglian Wendantang, and the positive control group was given 0.05 g·kg-1·d-1 aqueous solution of metformin hydrochloride, with the dose volume of 10 mL·kg-1·d-1. The blank group and the model group were given the same volume of distilled water. After continuous intragastric administration for 4 weeks, the body weight, body length and abdominal circumferences were measured, the Lee's obesity index was calculated, and the levels of fasting plasma glucose (FPG) and 2-hour plasma glucose (2 h PG) were detected in each group. Serum interleukin-6 (IL-6) content was detected by enzyme-linked immunosorbent assay (ELISA). The mRNA and protein expressions of nuclear factor kappa B (NF-
4.Investigation on the safety of regular plasma donors aged 56-60 years
Yang GAO ; Kecheng DONG ; Mingli MA ; Yan DONG ; Donghong FANG ; Yumeng SU ; Rong ZHOU ; Demei DONG
Chinese Journal of Blood Transfusion 2024;37(7):812-815
【Objective】 To investigate the safety of regular plasma donors aged 55 to 60, so as to provide reference for retention and recruitment of elderly plasma donors in China. 【Methods】 Plasma donors from 9 blood products manufacturing enterprises from 2018 to 2020 and the local general population were selected as the research objects. The total protein level, albumin and globulin ratio(ALB/GLB, A/G) and adverse reactions of plasma donation of regular plasma donors and local general population were retrospectively analyzed. 【Results】 The total protein level (g/L) and A/G of plasma donors aged 56 to 60 and the general population were 61.21±5.62 vs 60.04±6.93 and 1.610±0.299 vs 1.635±0.330, respectively, and the differences were statistically significant. The total protein level of regular plasma donors was higher than that of general population, but A/G was slightly lower than that of general population. From 2018 to 2020, there were a total of 23 056 302 plasma donations in 108 plasma stations, and adverse reactions occurred in 20 932 donations, with a total incidence of 0.09%, with no serious adverse reactions. 【Conclusion】 It is safe for regular plasma donors aged 55 to 60 to donate plasma, and the retention of them can alleviate the pressure of plasma supply.