Foreign Bodies ; pathology ; Humans ; Neck ; pathology ; Pharynx ; pathology

OBJECTIVE: To investigate the clinicopathological significance of the cyclin E expression in laryngeal squamous cell carcinomas (LSCCs). METHOD: The expression of cyclin E was detected by immunohistochemistry method in 50 cases of LSCCs, 20 cases of dysplastic squamous epithelium and 10 polyps of vocal cord. RESULT: The positive expression of cyclin E was localized in the nuclei of cells. The positive rate of cyclin E protein in LSCCs, dysplastic squamous epithelium and polyps of vocal cord was 10% (1/10), 15% (3/20), 6% (3/50), respectively. There's no significant difference between them. While the expression of cyclin E in laryngeal cancer stromal vascular endothelial cells was significantly related with clinical stage and lymph node metastasis (P<0.01). CONCLUSION In LSCCs, the expression of cyclin E has non-specificity, probably it has no participate the malignant transform events of laryngeal cancer cells. However, cyclin E is sensitive to vasculogenesis relatively; the positive expression of cyclin E may evaluate the malignant phase of LSCCs indirectly.
Adult ; Aged ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Cyclin E ; metabolism ; Female ; Humans ; Laryngeal Neoplasms ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis

OBJECTIVE: To explore the method of the controlled synechiae technique to maintain the stability of middle turbinate (MT), reduce the incidence of middle meatus synechia formation and improve the treatment effect of endoscopy surgery. METHOD: Eighty-six patients with chronic sinusitis were randomly divided into control group and treatment group in this study. In control group, the patients received extended nasal packing in middle meatus until 1 week after surgery. In treatment group, the patients received the controlled synechiae technique. RESULT: The MT position was described as stable, slight drifting laterally and synechia formation. And the incidence of synechia between MT and the nasal lateral wall was 29.4% and 14.9% in control group and treatment group,respectively. The differences were significant (P < 0.05). CONCLUSION For those patients with anatomic variation, destruction or weak supporting structures resulted from previous surgery, the controlled synechiae technique is very useful in preventing lateralization of the middle turbinate after endoscopy surgery.
Adult ; Aged ; Endoscopy ; methods ; Female ; Humans ; Male ; Middle Aged ; Nasal Surgical Procedures ; methods ; Postoperative Complications ; prevention & control ; Suture Techniques ; Tissue Adhesions ; Turbinates ; surgery ; Young Adult

OBJECTIVE: The aim of this study was to compare the sensitivity of conventional culture and PCR method. METHOD: DNA samples were collected from the sinus of 20 patients with chronic sinusitis and 18 patients diagnosed as fungal sinusitis clinically. Fungal specific PCR analysis and standard culture were performed on every sample. chi2 analysis was used to determine the statistical differences between groups. RESULT: Fungal DNA were detected in 78% of patients with fungal sinusitis and 5% of patients with chronic sinusitis in PCR analysis, while the positive rate were 44% and 0% in standard cultures respectively. CONCLUSION PCR is more rapid and sensitive than nasal swab culture in detecting the fungi in sinus mucosa. Our study suggests that it is an efficient method in diagnosis of fungal sinusitis.
Adult ; Chronic Disease ; DNA, Fungal ; analysis ; Fungi ; genetics ; isolation & purification ; Humans ; Middle Aged ; Paranasal Sinuses ; microbiology ; Polymerase Chain Reaction ; methods ; Sinusitis ; microbiology ; Young Adult