Objective To detect the expression of Beclin1 and Bcl2 in invasive pituitary adenomas and to explore the relationship of Beclin1 and Bci2 in invasive pituitary adenomas and the relativity between the 2 genes.Methods 61 specimens were classified into invasive group (32 cases) and non-invasive group (29 cases) according to the comprehensive evaluation of invasive pituitary adenomas.lmmunofluorescence analysis and RT-PCR were adopted respectively to detect the protein and mRNA expressions of Beclinl and Bcl2.The difference and relativity of Beclin1 and Bcl2 expression in invasive group and non-invasive group were analyzed.Results 32 specimens of pituitary adenoma were invasive and 29 were non-invasive.Beclin1 protein and mRNA expressions were lower in the invasive group than in the non-invasive group (P ＜0.01 ).Bcl2 protein and mRNA expressions were higher in the invasive group than in the non-invasive group (P ＜0.01 ).Pearson related analysis showed that Beclin1 mRNA expression was negtively correlated with Bcl2 mRNA expression in the invasive group ( r =-0.42,P =0.028 ).Conclusions Beclinl expression is decreased in invasive pituitary adenomas.The invasiveness of pituitary adenoma is closely related to the high expression of Bcl2 protein and mRNA,and the low expression of Beclin1 protein and mRNA.The inhibition of the autophagy may lead to the enhancement of the invasiveness of pituitary adenomas and that inhibition may come from the interaction of Beclin1 and Bcl2.

Objective To investigate the pathological mechanism of spinal injury by axial compression experiment on animal spine, so as to provide references for the treatment, prevention and research of spinal injury. Methods The biomechanical study of rabbit spine segments was performed by axial segment compression experiment. The compression process was recorded and strain analysis was performed by digital image correlation (DIC) technology. Results From the top to the bottom of the spine, the ultimate load and bearing capacity of the segment increased continuously; the average limit load of the corresponding single vertebral body was significantly larger than the segment; the strain of the intervertebral disc in the horizontal and vertical directions was significantly larger than that of the upper and lower vertebral bodies. Conclusions In the process of spine compression, the bearing capacity of the intervertebral disc should be taken into account and the injury of spinal segments is mainly manifested as abnormality of the intervertebral disc. The research findings contribute to the prevention and treatment of spinal compression fractures, as well as the design of related therapeutic instruments and assistive devices.