1.Comparison of tensionfree herniorrhaphy with conventional herniorrhaphy for inguinal hernia
Jiye LI ; Qun DENG ; Xinkui ZHANG ; Tao LI ; Shibin WANG ; Huabo JIAO ; Zhanliang LI
Chinese Journal of General Surgery 2001;0(08):-
ObjectiveTo evaluate the value of tensionfree herniorrhaphy for inguinal hernia. Methods(1)Comparison was made in operating time, early postoperative response, hospitalization time, postoperative complications and recurrence rate between the two herniorrhaphy groups.In tensionfree herniorrhaphy group( n=137),hernias were repaired with polypropylene mesh plug or mesh patch.(2)In conventional herniorrhaphy group (n=98 ) ,hernias were repaired with Bassini operation. Mean Follow up time was 22 month in the 2 groups. Results The operating time, analgesic use, early response and hospitaliz ation time in tensionfree herniorrhaphy group were significantly lower than those in conventional herniorrhaphy group(P
2.The effects of estrogen on the degenerative changes of rat condylar cartilage and subchondral bone
Tao YE ; Dongliang SUN ; Xili WENG ; Mian ZHANG ; Hongxu YANG ; Yichao LIU ; Shibin YU
Journal of Practical Stomatology 2018;34(1):5-10
Objective: To study the effects of estrogen on the degenerative changes of condylar cartilage and subchondral bone in rats. Methods: 18 female SD rats aged 6 weeks were divided into control(C),unilateral anterior cross-bite(UAC) and UAC treated with estrogen(UAC + E) groups(n = 6). UAC metal prosthesis was cemented to the left incisors of maxilla and mandible of the rats in group UAC and UAC + E. Rats in UAC + E group were given pexitoneal injection of 80 μg 17β-estradiol per day. The rats in group C were untreated. Animal were sacrificed at the 4th weeks. The micro-structure of subchondral bone was observed by Micro-CT scanning. HE staining,Safranin O staining,immunohistochemical staining,TUNEL staining and TRAP staining for the observation of pathological changes of histomorphology,extracellular matrix,chondrocyte apoptosis in condylar cartilage,and osteoclasts number in subchondral bone. Results: UAC and UAC + E group showed evident osteoarthritis(OA)-like lesions. Compare with UAC group,there was a significant decrease in the expression of proteoglycan(P < 0. 05),type Ⅱ collagen(P < 0. 01),and a significant increase in the number of apoptotic chondrocytes(P < 0. 01) in UAC + E group. As for subchondral bone,the BV/TV,Tb. Th parameters in C and UAC + E groups were significant higher than in UAC group(P < 0. 01),while the BS /BV,Tb. N,Tb. Sp parameters and the osteoclasts number in C and UAC + E groups were significant fewer than in UAC groups(P < 0. 01). There was no significant difference in bone ultra-parameters and osteoclasts number between C and UAC + E groups(P> 0. 05). Conclusion: In the model of rat TMJOA induced by unilateral anterior crossbite prosthesis,supra-physiological level of estrogen can reverse bone loss in subchondral bone,but accelerate the degradation of condylar cartilage.
3.Outcome comparison of sural neurofasciocutaneous flap for reconstructing soft tissue defects in forefoot and around ankle.
Lihong LIU ; Shibin TAO ; Zhonggen DONG ; Jianwei WEI ; Zhaobiao LUO ; Yu DAI
Journal of Central South University(Medical Sciences) 2022;47(1):79-85
OBJECTIVES:
To summarize our experience with the sural neurofasciocutaneous flap for reconstructing the soft tissue defects over the forefoot distal to the connecting line of midpoints in the metatarsal bones, and to compare the outcomes between the flap for resurfacing the defects distal and proximal to the connecting line.
METHODS:
The clinical data of 425 sural neurofasciocutaneous flaps for repairing the soft tissue defects in the middle and lower leg, ankle, and foot between Apr. 2002 and Apr. 2020 were reviewed. Based on the connecting line of midpoints of the metatarsals, the sural neurofasciocutaneous flaps were divided into a forefoot group (flaps with furthest edges distal to the connecting line) and a peri-ankle group (flaps with the furthest edges proximal to the connecting line).
RESULTS:
The partial necrosis rate in the forefoot group (14.5%, 10/69) was significantly higher than that in the peri-ankle group (7.0%, 25/356), with significant difference (P<0.05). Using the flap alone or in combination with a simple salvage treatment, the ratio of successful coverages of the defects was 98.6% (68/69) in the forefoot group, and 97.8% (348/356) in the peri-ankle group, respectively, with no statistical difference (P>0.05).
CONCLUSIONS
The sural neurofasciocutaneous flap is a better choice for covering the soft tissue defects over the forefoot distal to the connecting line of midpoints of the metatarsal bones. The survival reliability of the sural neurofasciocutaneous flap reconstructing the soft tissue defect proximal to the connecting line is superior to that of the flap reconstructing the defect distal to the connecting line.
Ankle/surgery*
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Humans
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Reconstructive Surgical Procedures
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Reproducibility of Results
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Soft Tissue Injuries/surgery*
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Surgical Flaps
4. Effect and mechanism of atorvastatin on cellular inflammatory response induced by calcium oxalate crystals
Yan SUN ; Zhiwei TAO ; Juening KANG ; Quan LIU ; Xiang WANG ; Shibin LONG ; Derong LI ; Yaoliang DENG
Chinese Journal of Urology 2019;40(10):780-785
Objective:
To investigate the effect and mechanism of atorvastatin (ATV) on the inflammatory response of human renal tubular epithelial cells (HK-2 cells) induced by calcium oxalate crystals.
Methods:
HK-2 cells were divided into control group (normal medium), ATV group (after 3 h pretreatment with 40 μmol/L ATV, replaced with normal medium), calcium oxalate crystal stimulation group (4 mmol/L calcium oxalate crystal) and ATV treatment group (after 3 h pretreatment with 40 μmol/L ATV, replaced with 4 mmol/L calcium oxalate crystals). After 12 h, the cells were collected, and the expression levels of NLRP3 and Cleaved caspase-1 were detected by immunohistochemical staining and Western blotting. The expression level of NF-κB was detected by immunofluorescence and Western blotting. The cell culture supernatant was collected to detecte the concentrations of interleukin-1β (IL-1β) and interleukin-18 (IL-18) by enzyme linked immunosorbent assay (ELISA).
Results:
Western blot analysis showed that the relative expression of NLRP3 (0.125±0.013 vs. 0.135±0.007) and Cleaved caspase-1 (0.090±0.014 vs. 0.095±0.006) was decreased in the ATV group compared with the control group, but the difference was not statistically significant (