1.Epidemiological investigation and phylogenetic analysis of Classical Swine Fever virus in Yunnan province from 2015 to 2021
Jun YAO ; Linlin SU ; Qiaoping WANG ; Lin GAO ; Jiarui XIE ; Yuwen HE ; Xianghua SHU ; Chunlian SONG ; Jun CHAI ; Yifang ZHANG ; Shibiao YANG
Journal of Veterinary Science 2022;23(4):e57-
Background:
Classical swine fever virus (CSFV), the causative agent of classical swine fever (CFS), is a highly contagious disease that poses a serious threat to Chinese pig populations.
Objectives:
Many provinces of China, such as Shandong, Henan, Hebei, Heilongjiang, and Liaoning provinces, have reported epidemics of CSFV, while the references to the epidemic of CSFV in Yunnan province are rare. This study examined the epidemic characteristics of the CSFV in Yunnan province.
Methods:
In this study, 326 tissue samples were collected from different regions in Yunnan province from 2015 to 2021. A reverse transcription-polymerase chain reaction (RT-PCR), sequences analysis, and phylogenetic analysis were performed for the pathogenic detection and analysis of these 326 clinical specimens.
Results:
Approximately 3.37% (11/326) of specimens tested positive for the CSFV by RTPCR, which is lower than that of other regions of China. Sequence analysis of the partial E2 sequences of eleven CSFV strains showed that they shared 89.0–100.0% nucleotide (nt) and 95.0–100.0% amino acid (aa) homology, respectively. Phylogenetic analysis showed that these novel isolates belonged to the subgenotypes 2.1c and 2.1d, with subgenotype 2.1c being predominant.
Conclusions
The CSFV was sporadic in China’s Yunnan province from 2015 to 2021. Both 2.1c and 2.1d subgenotypes were found in this region, but 2.1c was dominant.
2. Influencing factors of occupational stress among female workers in a large electronic manufacturing enterprise
Wenhui LIU ; Yali JIN ; Lichun LI ; Jiwu GENG ; Wei WEN ; Rongzong LI ; Huiqing CHEN ; Shibiao SHU
China Occupational Medicine 2017;44(05):604-609
OBJECTIVE: To analyze the influencing factors that cause occupational stress among female workers in a large electronic manufacturing enterprise. METHODS: A total of 1 081 female workers from a large electronic manufacturing enterprise were selected by the cluster random sampling method. The Chinese version of Job Content Questionnaire and Effort Reward Imbalance Questionnaire were used to assess occupational stress and its influencing factors were analyzed.RESULTS: Among 1 081 female workers,70. 95% and 19. 43% of them showed high level of occupational stress of job demand-control( JDC) model and effort-reward imbalance( ERI) model,respectively. Educational level with senior high school and above,assembly line working and exposure to occupational hazards were risk factors for high occupational stress of JDC model in female workers( P < 0. 01). The risk of high occupational stress ERI model in female workers with junior high school educational level and below was higher than that of senior high school educational level and above( P < 0. 01).CONCLUSION: The occupational stress rate of female workers in this electronic manufacturing enterprise is high. The main occupational stress model is JDC model. The main influencing factors of JDC model are educational level,operation mode and status of occupational hazard exposure.