1.The microRNA-21-5p regulation of type Ⅱ alveolar epithelial cell apoptosis
Ying HE ; Yun CHENG ; Lei SHI ; Zhouxiong XING ; Miao CHEN
Chinese Critical Care Medicine 2021;33(3):344-348
Objective:To explore whether microRNA-21-5p (miR-21-5p) has the effect of anti-apoptosis of human alveolar typeⅡ epithelial cells (ATⅡ).Methods:ATⅡ cells derived from the human were cultured in vitro and used for experiments when the cells were grown until the presence of lamellar bodies and microvilli were observed by light microscope. The cells were divided into blank control group (direct culture), hydrogen peroxide (H 2O 2) injury group (cultured with 0.5 mmol/L H 2O 2), and miR-21-5p overexpression group (using miR-21-5p with a multiplicity of infection (MOI) of 100 lentiviral overexpression vector with 0.5 mmol/L H 2O 2) and miR-21-5p empty virus control group (miR-21-5p lentiviral blank vector was co-cultured with 0.5 mmol/L H 2O 2). In each group, cell proliferation was detected by cell counting kit-8 (CCK-8) at 0, 12, 24, 36, and 48 hours of cell culture; cell apoptosis was detected by flow cytometry at 24 hours of culture. Results:① Cell proliferation activity test results: with the extension of cell culture time, the cell proliferation activity of the blank control group gradually increased, while the cell proliferation activity gradually decreased after the addition of 0.5 mmol/L H 2O 2. However, the cells proliferation activity in the miR-21-5p overexpression group decreased more slowly than that in the H 2O 2 injury group and the miR-21-5p empty virus control group, and the cell proliferation activity at 48 hours was significantly higher than the H 2O 2 injury group and the miR-21-5pempty virus control group ( A value: 0.295±0.005 vs. 0.184±0.005, 0.169±0.002, both P < 0.05). It showed that both H 2O 2 and lentivirus accelerated cell damage, while miR-21-5p could reduce cell apoptosis. ② Apoptosis rate test results: compared with the blank control group, the apoptosis rate increased significantly after adding 0.5 mmol/L H 2O 2; while the apoptosis rate of the miR-21-5p overexpression group was lower than that of the H 2O 2 injury group and miR-21-5p empty virus control group [early apoptosis rate: (14.31±0.12)% vs. (24.50±0.12)%, (23.41±0.13)%; late apoptosis rate: (8.12±0.13)% vs. (9.71±0.11)%, (10.41±0.15)%; overall apoptosis rate: (22.33±0.12)% vs. (34.21±0.10)%, (33.82±0.14)%; all P < 0.05], which further proved that miR-21-5p had anti-apoptotic effects. Conclusion:miR-21-5p has an anti-apoptotic effect on human ATⅡ.
2.Morphological characteristics of thoracic vertebrae in children aged from 10 to 12 years with three-dimensional digital reconstruction
Xiangwei LIU ; Xing WANG ; Shaojie ZHANG ; Zhijun LI ; Ying LIU ; Jun SHI
Chinese Journal of Tissue Engineering Research 2016;20(44):6620-6628
BACKGROUND:Articular process of joint is an important bony structure to maintain the spinal stability and normal physiological activities. Clinical study found that bilateral articular process asymmetry is also one of the causes of vertebral degeneration, but the existing research mainly focuses on the adult, and give priority to with cervical vertebra and lumbar vertebra. OBJECTIVE:To explore the morphological and developmental characteristics of thoracic facet joints and bony structures of 10 to 12-year-old children and compare with data of adults. METHODS:A total of 30 normal cases aging from 10 to 12 years were admitted into this study. No cases experienced bone destruction, deformity, fractures, or tumors, and spine surgery was not involved in. DICOM 3.0 data of multi-slice spiral CT (0.625-1.25 mm), ranging from T1 to T12, were used for three-dimensional reconstruction, measurement and statistical analysis. RESULTS AND CONCLUSION:(1) Joint width overal trends in“V”. Joint surface height from T1 to T12 increased gradual y with the increase of the operation sequence. (2) The joint thickness from T1 to T12 showed a gradual y increasing trend with the increase of the job sequence;articular process under the joint thickness was gentle. In the left and right sides of the upper and lower joints, joint surface width, height, and thickness did not show significant difference in addition to the significant difference between the individual vertebral body. Joints spacing on the side and there was no significant difference between upper and lower, articular process spacing between sides showed an increasing trend with the increase of operation sequence;upper and lower joints spacing increased with the job sequence, and showed wide“U”shape. (3) These results confirmed that the application of three-dimensional reconstruction techniques can display shape and characteristics of each vertebral body clearly and intuitively and improve measurement accuracy. Width and height of facet joints in 10-12-year-old school children were overal smal er than those of adults. The distance between upper and lower facet joints changed a little. Distance between left and right facet joints increased from T1 to T12, which complies with the growth and development of children.
3.PCNA, Bcl-2 and TERT expression in epiretinal membrane of rat traumatic proliferative vitreoretinopathy
Li-Ping, XUE ; Feng-Ying, KANG ; Shi-Xing, HU ; Xin-Guo, DENG ; Shao-Chun, LIN
International Eye Science 2005;5(1):19-22
· AIM: To explore the dynamic expression and correlation among telomerase catalytic subunit (TERT), proliferating cell nuclear antigen ( PCNA) and antiapoptosis protein Bd-2 which relate to cell proliferation in epiretinal membrane of rat traumatic proliferative vitreoretinopathy(PVR).· METHODS: S-P technique was applied for immunohistochemical staining of epiretinal membrane of traumatic PVR with TERT, PCNA and Bcl-2 antibody. HE staining was also carried out. The staining results were analyzed with image analysis system.· RESULTS: The positive rate and average A of PCNA protein were upregulated at first and then down-regulated, with the peak value in 14d Group, which was significantly different from those in 7d Group and 28d Group.The positive rate and average A of TERT and Bcl-2 were also upregulated at first and then down-regulated, with the peak value in 14d Group and 21d Group, which were significantly different from those in 7d Group. There was significant correlation among PCNA, Bcl-2 and TERT protein expression (P≤0.01).· CONCLUSTON: TERT and Bcl-2 take part in the regulation of proliferative cells in epiretinal membrane of traumatic proliferative PVR, with high correlation with the dynamic changes of cell proliferation.
4.Possible damaging effect of human cytomegalovirus infection on the kidney in children.
Shi-jun LI ; Xiao-yang YUAN ; Xing-yan BIAN ; Ying HAN
Chinese Journal of Pediatrics 2004;42(6):428-428
Biomarkers
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urine
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Child
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Child, Preschool
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Cystatin C
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Cystatins
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urine
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Cytomegalovirus Infections
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pathology
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urine
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Female
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Humans
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Kidney
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pathology
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Male
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TATA Box Binding Protein-Like Proteins
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urine
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alpha-Macroglobulins
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urine
5.Changes of P_(300) and Mismatch Negativity in the Treatment of First Episode Depression
hui, SU ; kai-da, JIANG ; fei-ying, LOU ; xing-shi, CHEN ; jian-hua, LIANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To study P300 of the first episode depression and mismatch negativity(MMN) changes after antidepressant treatment. Methods Sixty-four patients with first episode depression were evaluated by HAMD 17, and P300 and MMN tests were performed at the baseline and week 12. The cognitive potentials were compared with those of control group(N=36). Results Compared with the control group, depressive patients had longer latency of P300 and MMN,lower amplitude of P300 and MMN before treatment (P
6.Study on contingent negative variation in anxiety disorder
xin-kai, ZHANG ; ming-yuan, ZHANG ; jie-cheng, ZHAO ; fei-ying, LOU ; xing-shi, CHEN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(10):-
0.05)and significantly delayed reaction time,(305?109)ms vs(212?70)ms(P
7.Optimization of the detection method for urine S-phenylmercapturic acid via modified performance liquid chromatography-mass spectrometry
CUI Shi wei ZHOU Xiao ying XING Cai hong YAN Hui fang
China Occupational Medicine 2022;49(06):692-696
Objective -
To optimize the extraction and quantification methods for the determination of S phenylmercapturic acid
- Methods
(SPMA) in urine with performance liquid chromatography mass spectrometry. The urine was hydrolyzed with 50.0%
sulfuric acid. The hydrolysate was purified by solid phase extraction column. Purified samples were separated by C18
chromatographic column and detected by tandem mass spectrometry. The isotope labeled SPMA was used as the internal
Results -
standard. The internal standard curve was used for quantification. The linear range of SPMA was 0.50 50.00 μg/L with
the correlation coefficient of 0.999 8. The detection limit and the lower limit of quantification were 0.05 and 0.17 μg/L,
- - - -
respectively. The recovery rate was 97.0% 102.0%. The within run and between run relative standard deviation were 0.6% 1.0%
-
and 1.7% 6.5%, respectively. The mass concentration of urinary SPMA in the occupational benzene exposure group was
- vs P
higher than the non occupational benzene exposure group by this method (median: 2.81 0.28 μg/g creatinine, <0.05).
Conclusion
Compared to the national standard method, this optimized method of solid phase extraction and internal standard
for quantification eliminates the matrix effect. This method is accurate and precise, and is suitable for the determination of SPMA
acid in urine.
8.Problems and optimization of online book purchase recommendation system in academic library
Ying MA ; Xing LIU ; Chun-Zhuang YANG ; Dan SHI ; Li-Ying WU
Chinese Journal of Medical Library and Information Science 2017;26(12):64-67
After the current studies, processes and types of online book purchase recommendation system in academic library were briefly described,its major problems at present were analyzed with suggestions put forward for its optimization, including improvement of its functions, establishment of its dynamic knowledge repository, improvement of its timeliness, enforcement of its electronic resources purchase and importance attached to its performance assessment.
9.Long-term efficacy and adverse effects of intensity-modulated radiotherapy for nasopharyngeal carcinoma
Xiaomin OU ; Qi SHI ; Xin ZHOU ; Youqi YANG ; Xing XING ; Tingting XU ; Chunying SHEN ; Xiaoshen WAGN ; Lin KONG ; Xiayun HE ; Hongmei YING ; Chaosu HU
Chinese Journal of Radiation Oncology 2016;25(4):304-309
[Abstra ct] Objective To investigate the long-term efficacy and adverse effects of intensity-modulated radiotherapy (IMRT) for nasopharyngeal carcinoma (NPC).Methods A total of 869 patients with biopsy-proven NPC without distant metastasis who underwent the whole course of IMRT from 2009 to 2010 were enrolled.Of all the patients, 84.8%received cisplatin-based chemotherapy.The prescribed dose to the primary lesion in the nasopharynx was 66-70Gy in 30-32 fractions, and the dose to the positive lymph nodes in the neck was 66 Gy in 30-32 fractions.The Kaplan-Meier method was used to calculate survival rates, the log-rank test was used for difference analysis and univariate prognostic analysis , and the Cox proportional hazards model was used for multivariate prognostic analysis .Rseu lts The 5-year overall survival( OS ) , local recurrence-free survival, regional recurrence-free survival, distant metastasis-free survival, and disease-free survival ( DFS ) were 84.0%, 89.7%, 94.5%, 85.6%, and 76.3%, respectively.In the patients with locally advanced NPC,concurrent chemotherapy tended to reduce distant metastasis (83.6%vs.75.7%, P=0.050) and improve OS (82.6%vs.77.0 %, P=0.082).Induction chemotherapy tended to improve OS ( 80.7% vs.71.4%, P=0.057 ) , and the induction chemotherapy containing docetaxel or gemcitabine tended to improve OS (83.3%vs.72.2%, P=0.058).The patients who received a boost after the initial radiotherapy had a significantly lower DFS rate than those who did not (52.2%vs.71.1%, P=0.004).The concurrent chemotherapy increased the incidence rates of long-term xerostomia and trismus, while a high dose of cisplatin increased the incidence rates of xerostomia and hearing impairment.Conclusions IMRT for NPC provides satisfactory long-term efficacy.Concurrent chemotherapy combined with IMRT tends to reduce the incidence of distant metastasis, and other values need further investigation.The boost therapy after radiotherapy may be associated with poor prognosis.Chemotherapy increases the incidence of long-term toxicities.
10.Changes on the Polysomnography in Patients with Chronic Fatigue Syndrome
ming-dao, ZHANG ; hong-xing, WANG ; ze-ping, XIAO ; xiao-wei, LIU ; xing-shi, CHEN ; fei-ying, LOU ; ming-yi, TAO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To assess the changes on the whole night polysomnography (PSG) in patients with chronic fatigue syndrome(CFS). Methods The whole night PSGs were recorded from 24 patients with CFS and 33 normal subjects. Results Compared with normal subjects, patients with CFS showed significantly reduced total sleep duration ([488.7?21.7]min vs [515.9?31.7]min, P