Objective To study the expression of hyaluronidase(HAase) and matrix metalloproteinase-9(MMP-9) in the invasion and metastasis of human breast cancer.Methods The expression of HAase and MMP-9 in human breast cancer was detected using tissue homogenate preparing, enzyme-linked immunoadsorbent assay(ELSA),and immunohistochemistry examination.Results ①The levels of HAase were 4.89?2.55,8.03?2.66,and 12.00?3.96 mU/g in grade Ⅰ,Ⅱ,and Ⅲ of breast cancer,respectively(n=10,20,3),with significant differences between each other(P

Objective In order to provide important theoretical basis for further improving design of DC-7/7A high altitude partial pressure suit and FTF-2 air ventilation suit (named as A suit and B suit), the performance of ventilating heat dissipation of DC-7/7A and FTF-2 was studied. Methods Five healthy volunteers were enrolled to took participate in A group and B group tests. Environmental weather conditions for A and B suits were set at Tdb 40℃ and 45℃,rh 45% and 40% respectively, and the test time was 90min and 120 min respectively. Results During the test for A group and B group, Ts ranged from 31.5℃ to 34.5℃ for human comfort. Average skin temperatures of the lower part of the body were from 33.1℃ to 33.8℃. Skin temperatures of the legs did not obviously change or slightly lowered. Heart rate showed an increase of 12 beats/min and 14 beats/min. Sweat quantities were measured to be 0.288?0.056kg/1.5h and 0.445kg?0.077 kg/2h respectively. Evaporation rates were 77.4?3.6% and 84.2?2.4% respectively. Subjective uncomfortable sensation was more obvious as test time went on. Conclusion The results of the ventilating heat dissipation for both groups were quite good, but the findings suggested that the number of the ventilating holes for some important sites should shall be re-considered. Pilots wearing A group and B group with ventilation function would still suffer from mild heat stress and obvious subjective discomfort in hot enviroment.

Objective To explore the difference in pulmonary function between chronic obstructive pulmonary disease (COPD) and overlap syndrome patients.Methods 50 hospitalized COPD patients who were randomly recruited were divided into two groups, which contained 33 COPD patients and 17 overlap syndrome patients respectively. All the 50 patients’pulmonary function was evaluated and the results were analyzed statistically.Results The pulmonary function test showed that only PEF and V75 significantly decreased in overlap syndrome patients compared with COPD patients(P=0 032 and 0 048 respectively),But pulmonary volume, small airway function and pulmonary diffusion function in 5 COPD patients with severe sleep apnea syndrom (SAS) were significantly reduced compared with COPD patients.Conclusions Upper airway obstruction in SAS patients aggravates the pulmonary ventilation function in overlap syndrome. And obesity deteriorates both of the pulmonary function and hypoxemia in patients suffered from COPD.

Objective To study the effect of hyaluronidase and VEGF on human breast cancer invasion and metastasis. Methods By means of ELSA and immunohistochemistry, hyaluronidase and VEGF were detected in breast cancer. Results Hyaluronidase were (3.99?1.91) mU/g, (8.01?2.59) mU/g and (12.00?3.96) mU/g in gradeⅠ, Ⅱ, Ⅲ of breast cancer respectively. Hyaluronidase levels were significantly elevated in grade Ⅱ,Ⅲ as compared to grade Ⅰ, Hyaluronidase levels were significantly elevated in grade Ⅲ as compared to grade Ⅱ(P

Aim To observe the effect of valsartan on expression of Smads of HKCs stimulated by TGF-?1.Methods HKCs were divided into three groups:control group,TGF-?1 group and valsartan group.Total protein was firstly abstracted at 30 min after stimulation to detect p-Smad2/3 protein by Western blot.Total RNA and protein were abstracted at 48 hours after stimulation.The expressions of Smad2/3,Smad7 were measured by Western blot.Smad2mRNA and Smad7mRNA were measured by RT-PCR.Meanwhile, The protein synthesis of Col Ⅰ in the supernatants of the HKCs was detected by Western blot.MTT assay was used to investigate the effect of valsartan on proliferation of HKCs stimulated by 5 ?g?L-1 TGF-?1 at different time and different drug concentration.Results Western blot analysis indicated that TGF-?1 stimulation could increase the expression of Smad2/3 and activate phosphorylation of Smad2/3 at 48 hour.At the same time,Smad7 decreased.Valsartan could reduce the level of Smad2/3 and p-Smad2/3 but it did not have any effect on the expression of Smad7 protein.RT-PCR analysis also showed that the expression of Smad2mRNA or Smad7mRNA was higher after TGF-?1 stimulation than control group,while valsartan could down-regulate the expression of Smad2mRNA.There was no difference of the expression of Smad7mRNA between the TGF-?1 stimulation group and valsartan treatment group.Valsartan could also greatly ameliorate the secretion of Col Ⅰ in the supernatants of the HKCs stimulated by TGF-?1 detected by Western blot.Compared with control group,TGF-?1 supressed the proliferation of HKCs,While 1,10 and 100 ?mol?L-1 valsartan ameliorated it and the effect was dependent of dose.Conclusion Valsartan has some renal protective effects on renal interstitial fibrosis,partly through inhibiting TGF-?/Smads signaling pathway.

Objective To evaluate diagnostic significance of renal arteriography by injecting adrenalin in patients with renal tumour.Methods The renal angiography after administration of adrenalin (6 ?g) in 47 patients with renal malign ant tumour were performed.The angiographic results were analysed in comparison with that of pathology.The diagnostic accuracy parameters including sensitivity,specificity,accuracy,positive prediction rate,negative prediction rate,positive and negative index were caluclated.Results The sensitivity, specificity and accuracy in the detection of renal malign ant tumour were 93% ,94% and 94% respectively. Positive prediction rate were 97%,negative prediction rate was 89%, positive likelihood rate was 15.8, negative likelihood rate was 0.07 and Youden index was 0.87.Conclusion Medicaments angiography by injecting adrenalin is of important diagnostic value for renal tumour,especially for those which are vascularized and could not diagnosed by other technology qualitatively.

Objective To find out the possible regularity and mechanism of the adaptable change of human being T lymphocytes for physical exercise with oxygen and bring the original data for the Movement of All People Improving their Health. Methods We selected 16 untrained female students in university and let them had the same amount of exercise for 8 weeks. After that, we collected the cycle blood at the time point of before exercise, the end of exercise and 1 hour after exercise at the end of the 0,first,2 nd,4 nd,6 nd and 8 nd week respectively, so as to determine its stimuli index (SI) by MTT method. Results In the different time sect, such as the early stage of exercise, quiet condition,as soon as the end of exercise and 1 hour after exercise, we found that the SI were obviously Iower than that of normal (P＜0. 05) ,especially in the time sect of the end of exercise. Continuing to 4 weeks,the function of T lymphocytes restored gradualy and it lasted to the 8 th week, the SI in quiet condition and 1 hour after exercise had restored to normal(P＞0.05),but in the end of exercise, it still was Iow,however, the extent of the cases selected was in a condition of acute excitability. Conclusion As the bodies adapting to the exercise, the function of T lymphocytes restored slowly and the rate increased faster and faster.

The purpose of this paper is to investigate the reversal effect of small interfering RNA (siRNA) targeting MDR1 and MDR3 genes on the resistance of MCF-7/ADR cells to adriamycin. siRNA plasmid vector targeting MDR1 and MDR3 genes was transfected into MCF-7/ADR cells, and then was stained with Annexin-V FITC (fluorescein isothiocyanate conjugated) to detect the early stage cell apoptosis by flow cytometry (FCM). 50% inhibition concentration (IC50) of adriamycin for MCF-7/ADR cells was determined by MTT method. MDR1 and MDR3 mRNA was assessed by RT-PCR. Treatment of MCF-7/ADR cells with the two kinds of siRNAs resulted in a reversal of adriamycin resistance of MDR to different extents. 1) The apoptosis efficiency of MDR1 and MDR3 siRNA vector after transfection was (18.21+/-1.65) % and (9.07+/-2.16) % respectively (P<0.05), and there was significant differences in the apoptosis efficiency between pSuppressor Neo vector and the MDR1siRNA or MDR3 siRNA vector (P<0.01); 2) The reversal effect of MDR1 siRNA is higher than that of MDR3 siRNA (P<0.05); 3) The expression of MDRI and MDR3 mRNA can be restrained by pSuppressor Neo MDR1 and MDR3 siRNA respectively, and the reduction in the mRNA level was in a time-dependent manner (P<0.01). MDR1 and MDR3 gene silencing can enhance intracellular adriamycin accumulation in MCF-7/ADR cells, improve sensitivity of MCF-7/ADR cells to adriamycin, and induce cell apoptosis. The reversal effect of adriamycin resistance by siRNA of MDR1 was more effective than that of MDR3.

Objective To analyse the effect of berberine on D-dimer, fibrinogen (FIB) and interleukin-6(IL-6) in peripheral blood of ovarian cancer patients.Methods 130 cases selected from the hospital were diagnosed as unilateral ovarian cancer, treated by surgery and chemotherapy.The patients were divided into control group (25 cases) and experimental group (105 cases), and the experimental group were randomly divided into A, B, C three groups, 35 cases in each group.The experimental group received berberine hydrochloride tablets, orally, three times a day, on the basis of conventional chemotherapy.The experimental groups were divided into three subgroups according to the dosage: A group: 0.3 g, B group: 0.2 g, C group:0.1 g, 35 cases in each group, continuously treated for 2 weeks.The levels of D-dimer, FIB and IL-6 and quality of life score were detected before and after treatment.Results After treatment, D-dimer level in each subgroup of experimental group was lower than that in control group, respectively, and A group