[Objective]To observe the histomorphology and ultrastructure of the intervertebral disc(I D) in the rat model with cervical vertebral unbalance of dynamic and static force.[Method]Sixty SD rates were randomly divided into 4 groups: 3 months,5 months,7 months and the control group.The cervical I D degeneration model was made by destroying the neck muscle of rats and the tissues wer collected every month.The number,area and thickness of the cartilage end-plate were measured by the means of Miyamoto's Classes.The ultrastructure of the apoptosis cells of the intervertebral disc was observed under the electron microscope.[Result]Compared with the control group,the 3 months group showed degeneratin changes,with disordered structure of the annular fibrosis and thickness increase of calcification layer and decrease of blood vessel unmber in the cartilage layer.Complete fibrosis was found in nucleus pulposus in 5 months model group,with the fibro lamellar structure disappeared and few blood vessel buds.The features of 7 months model group was similar to 5 months and osteophyma had been formed near the bordr of part intervertebal.Under the electron microscope,the number of surface projection and organelles was decreased.Fatty drop and apoptotic body could be seen in disc cells of 3 month model group.Few cells,broken collagen fibers in ECM and more cavitation cells for necrosis in 5 months and 7months model groups could be seen.[Conclusion]The cervical I D of model groups has shown typical morphological changes of degeneration and this trend was more serious with the time passing.In the early and middle stage of degeneration,the apoptosis cells can be seen,but in the terminal stage the cellular necrosis was more common.

Objective To observe the effect of sitagliptin combined with insulin aspart 30 in the treatment of secondary failure of sulphonylurea in type 2 diabetes mellitus. Methods Fifty-six cases were divided into group A and group B in random block design, with 28 cases of each group. The patients in group A was treated with sitagliptin combined with insulin aspart 30, while the patients in group B was given subcutaneous injection of insulin aspart 30R. All patients were treated for 12 weeks. Fasting plasma glucose(FPG), 2-hour postprandial plasma glucose(2 hPG), glycosylated hemeglobin(HbA1c), insulin secretion index (HOMA-β), body mass index (BMI), and incidence of low blood glucose before and after treatment were compared. Results Compared with that in group B, FPG [(5.61 ± 1.14) mmol/L vs. (7.8 ± 1.22) mmol/L], 2 hPG [(7.62 ± 1.35) mmol/L vs(9.72 ± 1.41) mmol/L] and HbA1c [(7.11 ± 0.83)%vs.(8.32 ± 1.04)%] in group A had a significant decrease;HOMA-β[(50.31 ± 5.12) vs. (41.86 ± 4.53)] of group A was higher than that of group B (P

Combined with the five Zang Yin-Yang theory, to discuss the spinal marrow and cerebral Yin Yang state. As the internal organs, we believe that the spinal marrow and cerebral are substantial Yin and functional Yang. Further we can diagnose and treat the cerebral and spinal marrow injury related diseases. In the clinical therapy of acute and chronic cerebral and spinal marrow diseases, we should maintain the substantial Yin by avoiding injury, preventing spinal degeneration and maintaining the blood supply; and we should adjusting the functional Yang by nourishing the blood and promoting blood circulation, calming the liver to stop the wind, keep Yin and Weiqi in balance, tonifying the spleen and kidney etc. From the author's experience ,the patient with acute cerebral and spinal marrow injury diseases should be treated by regulating lung, liver, spleen and kidney to remove the wet water and purge the fire;the patient with chronic cerebral and spinal marrow injury diseases should be treated by regulating heart, liver, spleen and kidney to enhance Yang and nourish Yin.

0.05).Conclusion: rhGH can stimulate the growth of GHR2+ tumor cell lines such as SGC-7901 and weaken the inhibitory effect of 5-FU on GHR2+ tumor cells.However,such effect was not remarked for GHR+ tumor cells in vitro.

Asthma ; physiopathology ; Child ; Female ; Humans ; Male ; Maximal Expiratory Flow-Volume Curves ; Oscillometry ; methods ; Pulmonary Disease, Chronic Obstructive ; physiopathology ; Respiratory Function Tests

Objective To establish a platinum resistance nude mice model of epithelial ovarian cancer (EOC) and investigate its resistance to cisplatin (DDP) biological characteristics, so as to provide evidences for exploring chemoresistence mechanisms and screening for reversal targets in vivo micro-environment. Methods The resistance model was produced by repeating a crossover subcutaneous injection of human ovarian cancer SKOV3 cells labelled green fluorescent protein(GFP) and transplatation of tumor fragment into nude mice. Two kinds of cancer cell lines of SKOV3/DDPⅠand SKOV3/DDPⅡwere induced with acquired resistence to DDP. The chemosensitivities of EOC cells to DDP were tested and half maximal inhibitory concentration(IC50) was measured by methyl thiazolyl tetrazolium (MTT) and flow cytometry (FCS). Dynamic analysis among the concentration of DDP treatment and cell apoptosis, cell cycle phase distribution and intracellular DDP concentration. The expression of PTEN, STAT5, XIAP, BRCA1 and MDR1 were examined by real time quantitative reverser transcription PCR (qRT-PCR) in vivo. Results IC50 value of cisplatin for SKOV3/DDPⅡ were 2.83 ± 0.12 and 3.82 ± 0.19 folds than those for SKOV3/GFP by MTT and flow cytometry, separately. SKOV3/DDPⅠwere 2.20±0.16 and 3.40±0.20 folds. The apoptosis rate of SKOV3/DDPⅡ and SKOV3/DDPⅠ were decreased significantly at 29.7 and 39.6μmol/L DDP when treatment for 36 hours,which were lower than that of SKOV3/GFP cells [(57.0±1.4)%vs (37.6 ± 4.36)%vs (83.1 ± 2.71)%,P=0.024;(74.4 ± 2.3)%vs (50.5 ± 3.4)%vs (87.4 ± 4.0)%,P=0.001]. SKOV3/DDPⅠ and SKOV3/DDPⅡ was positively related with cisplatin processing time. Intracellular DDP accumulation of SKOV3/DDPⅡand SKOV3/DDPⅠwere lower than SKOV3-GFP in dynamic processes(P<0.05). Besides intracellular DDP accumulation of SKOV3/DDPⅡ also lower than SKOV3/DDPⅠin dynamic processes (P<0.05). Transplanted tumor of SKOV3/GFP appeared organelle degradation and nuclear membrane imcompleted after five times DDP injection with concentration of 4 mg/kg. SKOV3/DDPⅡand SKOV3/DDPⅠdid not generate these phenomenon untill eighth DDP injections with concentration of 4 mg/kg. STAT5 and BRCA1 of SKOV3/DDPⅡwere increased with DDP treatment at concentration of 4 mg/kg. Expression of XIAP from SKOV3/DDPⅡwas positive correlated with injection times. STAT5,XIAP and BRCA1 of SKOV3/DDPⅡwere up-regulated 3.86,28.1 and 14.6 folds than those in SKOV3/GFP cells after eighth DDP treatment, separately. While PTEN of SKOV3/DDP Ⅱ was decreased 3.77 folds. Conclusions We have successfully established platinum-resistent EOC mice model,which provides a new platform for further study on chemoresistant reversal and individualized clinical treatment. The results shown that potential mechanisms of SKOV3/DDPⅡDDP-resistance included over-expressed BRCA1 gene may be promote DNA damage repair, elevate XIAP gene to decrease cell apoptosis,up-regulated STAT5 gene and decrease PTEN gene to stimulate proliferation.

Adenocarcinoma ; metabolism ; secondary ; surgery ; Anal Canal ; chemistry ; pathology ; surgery ; Anus Neoplasms ; metabolism ; pathology ; surgery ; Carcinoembryonic Antigen ; analysis ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Keratin-20 ; analysis ; Male ; Middle Aged ; Mucin-1 ; analysis ; Paget Disease, Extramammary ; metabolism ; secretion ; surgery ; Skin Neoplasms ; metabolism ; secretion ; surgery

Carcinoembryonic Antigen ; metabolism ; Carcinoma, Squamous Cell ; metabolism ; pathology ; surgery ; Gastrectomy ; Hodgkin Disease ; pathology ; Humans ; Lymphoma, Large B-Cell, Diffuse ; pathology ; Male ; Middle Aged ; Mucin-1 ; metabolism ; Stomach Neoplasms ; metabolism ; pathology ; surgery

Cochlea ; metabolism ; Deafness ; genetics ; Humans ; Mutation

Objective To study the value of MR diffusion tensor imaging (MR-DTI)using a 3.0 T scanner in anterior ischemic optic neuropathy(AION).Methods A total of 26 patients suffering from unilateral subacute AION were examined using DTI and pattern visual evoked potential(P-VEP).The parameters values of optic nerves including fractional anisotropy (FA),ADC,vertical diffusivity (λ⊥),paralleldiffusion (λ//),P100 value and amplitude were obtained.Paired t-tests were used for comparing the FA,ADC,λ⊥,and λ// values of the two groups.Correlations of DTI parameters and P-VEP parameters were analysed by using the Pearson rank correlation analysis.Results The values of FA,ADC,λ//,and λ_ in the affected nerve were 0.28±0.07,(1.43±0.20) ×103 mm2/s,(1.79±0.16) ×10-3 mm2/s,and (1.25±0.17) ×10-3 mm2/s respectively,and the corresponding values of unaffected nerve were 0.57±0.05,(1.04±0.17) ×10-3 mm2/s,(1.71 ±0.19) × 10-3 mm2/s,and (0.75±0.08) × 10-3 mm2/s respectively.Compared to unaffected contralateral nerves,the mean FA was reduced,the mean ADC,λ⊥ were increased in the affected nerves(t=-19.269,10.537,15.301,P＜0.01).However,there were no significant difference of the λ//(t=l.632,P＞0.05).There was significant negative correlation between ADC and P-VEP amplitude in affected optic nerves (r=-0.722,P＜0.01).There was moderate negative correlation between λ⊥ and P-VEP amplitude in affected optic nerves (r=-0.634,P＜0.01).There was moderate correlation between FA and P-VEP amplitude (r=0.539,P＜0.01).There was no correlation between λ// and P-VEP amplitude.There was moderate negative correlation between FA and P-VEP P100 latency(r =-0.619,P＜0.01).Conclusions DTI can sensitively detect diffusional abnormality of anterior ischemic optic neuropathy.DTI could be used as a supplemental way in the assessment of AION.