AIM Interleukin 1?(IL-1?), which is a pivotal cytokine in inflammatory response, can inhibit human polymorphonuclear leukocyte (PMN) apoptosis,which can enhance PMN-mediated cytoxicity. Therefore we research the effect of IL-1? on the change of PMN functions was studied. METHODS Functional capacity of PMN was assessed by spreading, polarization (shape change), superoxide anion production and phagocytosis. RESULTS IL-1? can prolong PMN function by the inhibition of PMN apoptosis. CONCLUSION IL-1? inhibits PMN apoptosis, resulting in a larger population of surviving PMN with a greater capacity for phagocytosis and superoxide production. The later potentially facilitates PMN mediated tissue injury and may be one of mechanisms by which IL-1? contributes to organ dysfunction.

Child ; Humans ; Masturbation ; Medicine, Chinese Traditional ; Qi ; Syndrome ; Yin-Yang

Acupuncture Therapy ; Child ; Child, Preschool ; Drugs, Chinese Herbal ; therapeutic use ; Enuresis ; drug therapy ; etiology ; Female ; Humans ; Male ; Phytotherapy

Objective:To study the mechanism of effect of miR-21 via Wnt/β-catenin signaling pathway in human A549 lung cancer cells and Lewis lung carcinoma in mice. Methods:The effect of miR-21 on A549 cells were detected by MTT method. MiR-21 expression levels were overexpressed or inhibited in A549 cells by transfecting with miR-21 mimics or inhibitors. Correlation among key molecules (Wnt1,β-catenin, CyclinD1 and miR-21) of mRNA and protein levels in Wnt/β-catenin signaling pathway were studied by Real-time PCR and Western blot hybridization assay. Invasive ability of A549 cells was determined via Transwell chamber cell invasion assay;the role of miR-21 in A549 cells was explored via the Wnt/β-catenin signaling pathway. A Lewis lung carcinoma animal model was established to detect miR-21 expressions in tumor animals and controlled animal tissues, and verify expression changes of the above moleculesin the Wnt/ β-catenin signaling pathway was determined in the animal level. Results:MTT assay results showed that miR-21 overexpression could markedly enhance cell absorbance value;that is, miR-21 could increase the ability proliferation of A549 cells.β-catenin and CyclinD1 expression levels were significantly higher in miR-21 mimic transfected cells (P<0.05), and Wnt1 gene had no significant change. Wnt1,β-catenin and CyclinD1 gene expression showed no significant change when miR-21 expression was suppressed, compared with controls. After cells were transfected with miR-21 mimics, cell invasion assay revealed that the perforated cells was significantly higher than the perforated cells in the control group (P<0.01). Lewis lung assay revealed that miR-21 expression levels in the Lewis lung carcinoma were significantly higher;and at the same time, Wnt1,β-catenin and CyclinD1 gene expression levels were significantly increased, compared to controls. Conclusions: In A549 human lung cancer cells and Lewis lung carcinoma in mice, key moleculesβ-catenin and CyclinD1of miR-21 expressions and the Wnt/β-catenin signaling pathway are positively correlated.

Objective To measure the serum levels of TWEAK and survivin in rheumatoid arthritis (RA)patients and explore their clinical value. Methods The serum levels of TWEAK and survivin were measured by ELISA methods in 61 patients with RA and 35 healthy controls. The function stage, X-ray stage,and serum levels of ESR, RF and CRP were measured at the same time. Results There was no significant difference between the serum levels of TWEAK in patients with RA[(479±381)pg/ml]and control[(421±87 pg/ml]. The serum level of survivn in patients with RA[(159±82)pg/ml]was increased significantly than that of the controls[(119±24)pg/ml]and were associated with X-ray stage. Conclusion We need further research to explore the relationship between TWEAK and RA. The serum level of survivin is associated with chronic erosive RA and maybe a target for new therapeutic approach of RA.

Objective To investigate the association between FcγRIIA H/H131 genotype and pneumonia through a comprehensive meta‐analysis .Methods Databases including PubMed ,Embase ,and China National Knowledge Infrastructure (CNKI) were searched with such terms as “FcgammaRIIa” ,“FCGR2A” ,“Fcgamma receptor IIA” ,“CD32” and“pneumonia” ,“polymorphism” ,“variant” ,“genotype” ,“allotype” ,as well as“pneumonia”and“polymorphism”in Chinese to identify relevant studies .Two reviewers independently extracted the data .Crude odds ratios (ORs) and corresponding 95%confidence intervals (CIs) were estimated .Heterogeneity across studies and publication bias were evaluated and sensitivity analyses were performed .Results Six case‐control studies were finally included in this analysis .The results indicated an association between FcγRIIA H/H131 genotype and pneumonia susceptibility (OR 1 .67 , 95% CI 1 .11‐2 .51 ) , but no significant association with R/R genotype(OR 1 .09 ,95% CI 0 .85‐1 .40) or H/R genotype(OR 0 .81 ,95% CI 0 .60‐1 .19) . Statistically significant heterogeneity was found during data pooling (I2 was 75 .4 % ,51 .8% and 71 .9% ) .Sensitivity analyses further indicated the reliability of the study .Egger′s and Begg′s tests identified publication bias in R/R genotype ,but not in H/H or H/R genotype .Conclusions We preliminarily find an association between FcγRIIA H/H131 genotype and pneumonia susceptibility .

Objective To clone and construct the plasmid containing human thyroid stimulating hormone receptor(TSHR) gene ectodomain,and then identify the immunoreactivity of the purified recombinant protein.Methods TSHR total RNA was extracted from human thyroid,and cDNA was obtained with RT-PCR technique.Human TSHR ectodomain gene(hETSHR) was cloned into pcDNA3.1(+) vector.The recombined construct was transfected into CHO cells by Lipofectin 2000.The transcript mRNA was detected by RT-PCR,and protein immunoreactivity was assayed by TSHR antibody with immunocytochemistry staining and Western blot.Results DNA sequencing results showed that the recombinant of human thyrotropin receptor ectodomain had confirmed sequence reported in GenBank.The fusion protein had the immunoreactivity.Conclusion Human thyroid stimulating hormone receptor was successfully cloned in eukaryotic expression vector and the constructor was expressed in eukaryotic cells very well.

Objective To explore the relationship between relocation satisfaction and psychosocial stress of transitional peri-od immigrants in the south-to-north water diversion project .Methods Stratified multi-stage cluster sampling method was used in this study ,a basic questionnaire and Psychosocial Stress Survey for Groups(PSSG) were used to investigate transitional period im-migrants who moved to Xingyang and Baofeng from Zhechuan holding different subjective feelings of migration .Results (1) Ex-cept that the evaluation of the implementation of immigration policy existed significant differences(P< 0 .05) between the two are-as ,other evaluations didn′t exist significant differences .(2) There were significant differences (P< 0 .05) in negative emotions ,pos-itive emotions ,negative coping style ,total psychological stress score between the three groups about the different evaluations of new life satisfaction ,new residence satisfaction and overall relocation satisfaction in new residence .Other evaluations didn′t exist signifi-cant differences in psychological stress .(3) Decision tree indicated that the key factors from strong to weak which influence psycho-social stress were new residence satisfaction ,new life satisfaction and overall relocation satisfaction .Conclusion The overall level of psychological stress in immigration is more intense than reference ,which positive and negative nature of evaluation has a direct link with it .

Objective To summarize and discuss the diagnostic and treating experiences of chronic pancreatitis with mass in the head. Methods Eight patients of chronic pancreatitis with mass in the head who were misdiagnosised as carcinoma of head of pancreas were analyzed retrospectively in the past 10 years. Results All the patients exhibited abdominal pain,5 of whom were with jaundice and 3 with anorexia. All the patients were misdiagnosised as carcinoma of head of pancreas before the operation,but the pathology after operation indicated chronic pancreatitis. The pancreaticoduodenectomy was performed in 5 patients,the choledochojejunostomy in 2 patients,while the exploratory laparotomy in 1 patient. After the operations,the abdominal pain was relieved in 7 patients, while 2 patients who accepted pancreatoduodenectomy suffered from pancreatic fistula,1 of whom died in the end. Conclusion It’s hard to differentiate the chronic pancreatitis with mass in the head from the carcinoma of head of pancreas before operation. If the carcinoma of head of pancreas can’t be excluded during the operation,the pancreatoduodenectomy should be performed,while the duodenum-preserving total resection of the head of the pancreas or any intra-drainage operations should be done if chronic inflammation is found in the whole pancreas with a negative result of the biopsy of the pancreas through the needle aspiration.

Objective We sought to evaluate the effect of structured health education on quality of life and the end point of patients with coronary disease with evidence-based method.Methods The Cochrane Library,Cochrane Database of Systematic Reviews (CDSR),Medline (OVID),Pubmed and Web of Science were searched to collect the randomized controlled trials (RCT) which mentioned the structured health education to CHD patients,the quality of the RCTs was evaluated by Jadad Score,then effective data were extracted and analyzed by Meta-analysis with RevMan 5.2 software.Results A total of 12 eligible studies of RCT were included in the study with Jadad Score evaluations at B level or above.There were 20 476 cases in the intervention group,14 522 cases in the control group.Meta-analysis showed that the quality of life scores of the intervention group were significantly higher than those of the control group,weighted mean difference was 3.29,95％ CI (0.49-6.09).No significant difference was found in cardiac morbidity outcomes,re-hospitalization and the mortality rate,relative risk,95％ confidence interval were respectively 0.72(0.50-1.04),0.98(0.86-1.11),0.77(0.58-1.03).Conclusions The structured health education can improve the quality of life of patients with coronary disease,but has no obvious effect on the cardiac morbidity outcomes,re-hospitalization and mortality rate.We suggest transitional care to be carried out in future research to decrease the incidence rates of them.