Objective To analysis the correlation of Serum homocysteine ,vitamin B12 and folate levels and subclinical hypot‐hyroidism in pregnancy .Methods 150 cases of subclinical hypothyroidism during pregnancy were selected as the experimental group ,and 150 cases of pregnant women with normal thyroid function were collected as the control group .Compared the serum thy‐roid‐stimulating hormone ,free thyroid hormones ,three free triiodothyronine ,homocysteine ,serum vitamin B12 ,folic acid levels of pregnant women in the two groups ,and analyzed the related serum markers of thyroid‐stimulating hormone and serum homocys‐teine .Results The homocysteine levels in experimental group was higher than that of control group(P<0 .05) ,the vitamin B12 and folate levels in experimental group were lower than that of control group (P<0 .05) ,the thyroid‐stimulating hormone levels in ex‐perimental group was significantly higher than that of control group (P<0 .05) .Serum thyroid‐stimulating hormone and homocys‐teine levels were positively correlated (P<0 .05) ,serum thyroid‐stimulating hormone and vitamin B12 and folic acid had no signifi‐cant correlation (P>0 .05) .Serum homocysteine and thyroid stimulating hormone levels were positively correlated (P<0 .05) ,ser‐um homocysteine and folate levels were negatively correlated (P<0 .05) ,serum homocysteine levels and vitamin B12 were not corre‐lated (P> 0 .05) .Conclusion Subclinical hypothyroidism during pregnancy have some relevance with homocysteine ,vitamin B12 and folate levels .

Objective:To explore the effect of the solution-focused brief group counseling on relieving procrastination of graduate students.Methods:Totally 33 graduate students with procrastination [the score of Pure Procrastination Scale (PPS) was more than or equal to 3.5] were selected through the Internct.They were randomly divided into two groups,17 in the ordinary counseling group (ordinary group) and 16 in the solution-focused counseling group (SF group).Samples in the SF group were given the solution-focused brief group counseling,while the ordinary group was given ordinary group counseling.The General Self-efficacy Scale (GSES),Self-control Scale (SCS) and Pure Procrastination Scale (PPS) were used to get the outcome of graduate students at baseline,the time after the group counseling (1 week follow-up,1 month follow-up).Results:The interactive effect between group and time of SCS,PPS were statistically significant (Ps ＜0.01).Separate analyses showed that there was no significant difference between two groups in all variables at baseline (Ps ＞0.05).At the end of the group counseling,the SCS scores were higher in the SF group than in the Ordinary group,and the PPS scores were lower in the SF group than in the Ordinary group.Conclusion:It suggests that solution-focused brief group counseling could improve selfcontrol and relieve procrastination.

Objective To establish the molecular genetic assay for presymptometical diagnosis of Wilson disease(WD). Methods Chromosome haplotype of WD was constructed using three genetic markers.Disease-causing mutations of Wilson disease gene(WND) were examined among the WD probands and their sib by PCR-SSCP and DNA sequencing analysis in WD pedigrees, to expound whether the disease causing mutations of WD patients gene was simila to that of their probands.Results Two presymptomatical WD patients were diagnosed correctly in ten WD pedigrees, including one newborn girl. One carrier ,who has a lower serum ceruloplasmin(sCP) level,was discovered. Conclusions Presymptomatical WD diagnosis can be made by haplotype analysis and mutation examination.So that, WD patients can be properly treated in their early stage.

Shenjufencilu (SFL) is a liniment prepared from Dedsnthema mordelium,Angelica dahurica,Sophora fiavescens,Dictamnus dasycarpus and a mumber of other herbal midicines for the treatment of puberal acne.The presence of the above four contituents was identified by TLC and the content of matrine,the active principle from Sophora fiavescens was determined quantitatively by TLC scanning,to establish the quality standard of SFL. The methods were simple,sensitive,reproducible and accurate

Carcinoma, Squamous Cell ; genetics ; metabolism ; pathology ; Esophageal Neoplasms ; genetics ; metabolism ; pathology ; Gene Frequency ; Humans ; Lymphatic Metastasis ; Neoplasm Invasiveness ; Neoplasm Staging ; Polymorphism, Genetic ; Prognosis ; Proportional Hazards Models ; Risk Factors ; Survival Rate ; Tumor Necrosis Factor-alpha ; genetics ; metabolism

Objective To explore the expression of membrane form of CD28 (mCD28) on T lymphoeytes and the serum level of soluble CD28 (sCD28) in elderly patients with primary non-small cell lung cancer (NSCLC) in order to investigate the relationship between age-related changes of CD28 and the development of NSCLC in elderly patients. Methods 63 elderly patients with NSCLC, 35 elderly patients with lung benign lesion, 30 elderly healthy donors, 30 young healthy donors, 20 young patients with lung benign lesion and 20 young patients with NSCLC were enrolled in this study. The mCD28 on T cells and the serum level of sCD28 were measured by four-color flow eytometric assay and enzyme linked immunosorbent respectively, and the relationship between CD28 and clinical characteristics of NSCLC was analysed Results The expression of mCD28 was decreased and the serum level of sCD28 was increased in elderly patients with NSCLC compared with the other groups (F= 184.25, P<0. 01 ; F= 365.40, P<0.01). The expression of mCD28 was significantly lower and the level of sCD28 was significantly higher in elderly healthy donors than those in young healthy donors and young patients with lung benign lesion (P<0. 05). There were no significantly statistical differences in expression of mCD28 and level of sCD28 between elderly healthy donors and elderly patients with lung benign lesion [(42.84±5.82)% vs. (46.09±-7.34)%, (39.38±6.02)μg/L vs. (35.84±5.02)μg/L, P>0. 05]. Logistic regression analysis showed that aging (OR=2. 432), down-regulation of mCD28 expression (OR=0. 876) and up-regulation of sCD28 level (OR= 1. 113) were the risk factors for lung cancer. In the elderly patients with NSCLC, there were significant differences in mCD28 expression and sCD28 level between stages Ⅲ-Ⅳ and stages Ⅰ-Ⅱ [(16. 51± 5.64)% vs. (24.41±8.24)%, (75.03±5.98) μg/L vs. (66.73±7.52)μg/L; t=4.497,4.794, both P <0. 01]. However, there were no significantly statistical differences among different pathological types (F=0. 609, 0. 302, both P > 0. 05). Conclusions The down-regulation of mCD28 expression and up-regulation of sCD28 level with advancing age play an important role in the oncogenesis and development of primary non-small cell lung cancer in the elderly patients.

reliability, content validity, construct validity, and criterion - related validity of the CNS are entirely in accordance with the psychometric demands.

Objective To investigate the incidence and influencing factors of aldosterone escape in patients with non-diabetic nephropathy by RASI-therapy. Methods A total of 104 patients with non-diabetic nephropathy were treated with ARB or combination therapy of ACEI and ARB in a mean follow-up period of 12 months. Aldosterone escape was determined according to the change of plasma aldosterone concentration before and after treatment during 6-month and 12-month ACEI/ARB treatment, while the influencing factors of aldosterone escape in patients with non-diabetic nephropathy was also analyzed after therapy with RASI . Results In 12 months, the incidence of aldosterone escape was significantly higher than that in 6 months (26.92% vs. 14.42%, P = 0.007). After 12-month treatment, the difference was statistically significant in incidence of aldosterone escape among different stages of CKD (P = 0.027). Compared with 6-month incidence of aldosterone escape in the losartan group, 12-month incidence increased evidently (P = 0.020). The Ald level was positively correlated with urinary protein excretion and the Scr level (r = 0.431, P = 0.003 and r = 0.336, P = 0.009, respectively), and negetively correlated with levels of the eGFR (r = -0.275, P = 0.006). Univariate Logistic regression demonstrated that risk factors of aldosterone escape included pre-treatment values of the urinary protein excretion (OR = 3.671, P = 0.028) and the eGFR (OR = 0.972, P = 0.019). Multivariate Logistic model revealed pre-treatment values of the eGFR was positively associated with aldosterone escape (OR = 0.970, P = 0.012). Conclusion The incidence of the aldosterone escape increases along with the time of treatment. Renal function has correlated with aldosterone escape and pre-treatment value of the eGFR is an independent risk factor of aldosterone escape.

To foster the medical scientific and humanistic spirit of medical students,a cours "eOutline of medical humanities" was designed and implemented in the Third Military Medi-cal University. This course puts great emphasis on helping students understand the meaning of medical humanities,improving their judgment of the social and moral value of medicine,giving correct guidance to young students as to the essence of medicine,promoting the harmony of medical scientific spirit and medical humanistic spirit of the medical students and making tehm become qualified medical workers.

ObjectiveTo improve the teaching quality of medical humanity education in medical university.MethodsA cluster sampling survey was given to 448 sophomores who studied the outline of medical humanities course in military medical university,and the data were descriptively and statistically analyzed.ResultsThe survey shows that 90％ sophomores satisfy with this course,95％ sophomores understand the importance of medical humanity education,and the students have more requirements on education resources and teaching ways.ConclusionOutline of medical humanities course is important to cultivate the medical humanity spirit of medical students,and the course still needs more improvements.