The Weibel-Palade-like body were found in the cytoplasm of the growth hormone and follicullar - stellate cells of rabbit adenopypophsis. The body is surrounded by a single unit membrane. In this body, there are numerous of microtubules which can be divided into two groups depending upon their diameters.Yet, it is uncertain what the virtual function is and where the bodies come from. However, according to the experimental observations, it is suggested that the body serve as a mechanical power device which has relations with the regional distribution of the organelles. The intranuclear microtubules and intranuclear tubules were also found in the growth hormone cells. The diameters of these intranuclear microtubules range from 25 - 30 nm, and the intranuclear tubule about 78 nm. The fomer seems to be connected with the heterochromatin granules, and may be. a transitional pattern from heterochromatin to euchromatin, but their importance remain to be clarified.

The ultrastructural changes in growth hormone (GH) cells of rabbit adeno-hypophysis are determined quantitatively with stereological method after burn injury. Blood sugar levels are concurrently measured. The results show: the volume fraction of Golgi complex, mitochondria and rough endoplasmic reticulum shows a 2-phase curve after burn, and it may be divided into 3-periods stress, modulation and functional activation and recovery; the rate of maturation of secretory granules is increased; a close relation between the blood sugar and some activities of GH cell secretion after burn presents; the autocrine shows a negative feedback to the exocytosis in the control group,and a positive feedback in burned ones; the newly formed mitochondria may be originated from the new Golgi complex from the reutilization of plasma components.

Objective To evaluate the importance of the prenatal diagnosis to fetus with mild ventriculomegaly ( MVM) in pregnancy outcome and prognosis. Methods The ultrosonographic results of 51 pregnant women who were admitted to our hospital were collected in this study. In each case,a thorough evaluation of fetal anatomy was performed,karyotyping or MRI was done if needed,and follow-up was done un-til afterbirth. Results In 51 cases of lateral ventriculomegaly fetal:ultrasonography indicated there were 26 fetuses with mild ventriculomega-ly. The incidence of MVM complicated with other abnormities was 46. 0%,and that complicated with chromosome disorder was 11. 5%. Con-clusion Ultrasonography plays an important role in the detection of MVM. Chromosome analysis should be carried out for elder pregnant women when their fetuses are diagnosed as MVM and other complicating abnormities.

Food Contamination ; prevention & control ; Public Health Practice

Objective To explore the imaging features of pediatric splenic primary solitary tumor. Methods The medical imaging appearances of 6 children with splenic primary solitary tumor conifrmed by surgery and pathology from January 2010 to August 2015 were retrospectively studied. The related literatur were reviewed. Results In 6 children, 2 children of cystic lesions, ultrasound showed anechoic mass, CT scan showed low density mass, without change after contrast, pathologically conifrmed as congenital cyst;2 children’s ultrasound showed solid echo, color Doppler ultrasound explored the blood lfow signals, contrast CT showed the lower strength around the splenic parenchyma, pathologically conifrmed as splenic lymphangioma;one child’s unltrasound showed echo mass with rich signals of blood lfow, MRI scans showed T1WI, T2WI and other signals of lumps, with little homogenity after contrast, pathologically conifrmed as splenic hamartoma;one child’s ultrasound cannot distinguish tumor boundaries, contrast CT scan showed concentric filling reinforcement, pathologically confirmed as hemangioma. Conclusion Splenic primary solitary tumor in children is rare. There are no speciifc clinical characteristics. The image ifndings can deifne the locations and help the differential diagnosis. The ifnal diagnosis depends on pathology.

Objective To evaluate the sonography features of heptaobiliary involvement of pediatric Langerhans′cell histiocytosis ( LCH) . Methods Nine pediatric patients with LCH confirmed by the clinical presentation ,biochemical tests and pathologic results of our hospital were retrospectively analyzed . Results Eight patients were seen in multi‐system LCH ,while one patient only had liver involved . All patients showed varying hepatomegaly with multiple distributed lesions in ultrasound images . According the lesion distribution features ,they could be classified into three types:central type(6/9) ,peripheral type(1/9) and diffuse type(2/9) . In ultrasonography ,they manifested as linear lesions alongside the central portal veins , multi‐nodular and randomly distributed lesions in liver parenchyma ,or both of them . Eight bile‐duct involved patients behaved with unevenly thicked bile‐duct walls ,stenosis or dilated bile‐ducts ,and even sclerosing cholangitis , some of them with cholelithiasis . Conclusions Pediatric LCH patients with heptobiliary involved are seemed mainly in multi‐system LCH ,while it is rare but possible that pathological Langerhans′cell only infringe on liver and bile‐ducts . The ultrasound imaging findings of pediatric LCH liver infiltration are with characteristic and are helpful for the early diagnosis and clinical procedure .

Objective To discuss the application of visualization software of CiteSpaceⅢ to the treatment and research of transfusion medicine.Methods The software of CiteSpaceⅢ and the function of the reference database ISI Web of Science itself were used to the study.Results In the past 16 years,paper quantity and cited frequency on transfusion medicine re-search had the wave-like increasing tendency year by year.The research forces of the field were mainly distributed in Europe and the United States,the research hot spot and frontier around the blood safety,change over time in a dynamic develop-ment.Conclusion The study reveals the progress and development tendency of transfusion medicine,which could provide ef-fective reference for related research.

Objective To investigate the peripheral blood Th17 and CD4 + CD25 + regulatory T cell levels and their correlations in patients with primary hepatocellular carcinoma (PHC).Methods Peripheral blood samples were collected from 30 PHC patients and 25 healthy controls in the First Affiliated Hospital of Zhejiang University from June 2008 to May 2009.Mononuclear cells were isolated and the Th17 and CD4 + CD25 + regulatory T cells were detected by flow cytometry and compared between patients and controls by t test.Spearman test was performed to analyze the correlation of Th17 with CD4 + CD25 +regulatory T cell concentrations.Results The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood in healthy controls were (2.10 ± 0.87) ％ and (7.10 ± 2.32) ％ ; while those in PHC patients were (3.38±1.68)％ and (11.78±5.62)％ (t=3.640 and 4.162,P＜0.01).The level of Th17 cells was positively associated with that of CD4 + CD25 + regulatory T cells in PHC patients (r =0.821,P ＜0.01).Conclusion The levels of Th17 and CD4 + CD25 + regulatory T cells in peripheral blood are high in PHC patients and positively correlated with each other,which indicates that CD4 + CD25 + regulatory T cells may contribute to the disease progression and pathogenesis of carcinoma through inducing Th17 cells differentiation.

[Objective]To investigate the significance of the high-risk human papilloma virus (HPV)detection in the screening and diagnosis of cervical lesions.[Methods] The high-risk HPV DNA test results of 797 patients with cervical lesions who all accepted cytology and histopathology test were collected and analyzed retrospectively.[Results]The high-risk HPV DNA positive rates in cervicitis,cervical intraepithelial neoplasia(CIN)Ⅰ,CIN Ⅱ,CIN Ⅲ and cervical cancer were 53.41％(188/352),70.91％(117/165),87.63％(85/97),97.90％(140/143),97.50％(39/40),respectively.The sensitivity and negative predictive value of the high-risk HPV DNA detection for CIN Ⅱ and more serious lesions were 96.66％(318/329),93.29％(153/164),respectively.The detection rate of CIN Ⅱ and more serious lesions in patients with atypical squamous cells of undetermined significance(ASCUS)and positive high-risk HPV DNA was 30.03％(94/313),while the rate in patients with negative high-risk HPV DNA was 1.55％(2/129).[Conclusions] The more serious the cervical lesion is,the higher high-risk HPV DNA positive rate is.It is most closely related with CIN 11 and cervical cancer.The high-risk HPV DNA detection has high sensitivity and negative predictive value for CIN Ⅱ and more serious lesions.The high-risk HPV DNA detection has high negative predictive value in CIN Ⅱ and more serious lesions in ASCUS.

Objective: To identify the mutation points of Cu/Zn superoxide dismutase(SOD1) gene in an amyotrophic lateral sclerosis(ALS) family with a unique phenotype,and to compare the value of single strand conformation polymorphism(SSCP) and denaturing high performance liquid chromatography(DHPLC). Methods: Five exons of SOD1 gene were amplified by PCR. The difference of these products were analyzed by PCR-SSCP and DHPLC.DNA sequencing was used to examine the mutation. Results: ①Mutations were found in exons 2 and 5 in several family members.DNA sequencing revealed that a base pair insertion occurred in the codon area of exon 2 and in the non-codon area of exon 5.②The results of DHPLC tests proved double peaks in one member with ALS symptoms(Ⅲ1),which indicated the possibility of mutation in SOD1 exon 4.DNA sequencing revealed that there was a heterozygote,with a mutation of GAA to GGA in exon 4 in the member with double peak. Conclusion: ①The mutations in exons 2,4,5 were proved.Insertion of exon 2 may be responsible for the disease of the ALS family in Chongqing.②Compared with PCR-SSCP,DHPLC technique has been proven to be a rapid and reliable method for screening mutation site in large samples.