Arteries ; surgery ; Arteriosclerosis Obliterans ; surgery ; Bioprosthesis ; Blood Vessel Prosthesis ; Blood Vessel Prosthesis Implantation ; instrumentation ; Humans ; Lower Extremity ; blood supply ; surgery

Objective To study the suitable dose of intracoronary adenosine (AD) on fractional flow reserve (FFR) measurement in Chinese patients with angiographic coronary artery disease. Methods FFR was measured in 32 patients with 40 moderate coronary stenosis. Boluses of intracoronary AD at increasing doses of 60μg (A1), 80μg (A2), 100μg (A3) and 120μg (A4) were randomly administered. FFR values, symptoms, systemic effects and development of atrioventricular block were recorded. Results FFR value decreased significantly by 8.99%(A1), 11.24%(A2), 13.48%(A3) and 13.48%(A4) compared with the baseline distal coronary pressure/aortic pressure (0.891±0.044, all P<0.001). A3 and A4 showed significantly lower FFR values than A1 (t=6.331、6.343, all P < 0.001),A2 (t=2.974、3.058, P=0.005、0.004). Positive rates of an FFR of<0.75 were 30.0%(n=12), 32.5%(n=13), 35.0%(n=14) and 35.0%(n=14) in A1, A2, A3 and A4. A total of 13 patients(40.6%) reported at least one side effects. Conclusions This study suggests a dose-response relationship for intracoronary AD on the measurement of FFR in Chinese patients with angiographic coronary artery disease. The suitable dose of bolus of intracoronary AD is 100μg.

BACKGROUND:Both of acupuncture and mesenchymal stem cel transplantation have therapeutic effects on cerebral palsy. OBJECTIVE:To explore the therapentic effect of acupuncture combined with placenta derived mesenchymal stem cel s on rat cerebral palsy. METHODS:Thirty-five Wistar rats were equivalently randomized into five groups, including control group, model group, acupuncture group, transplantation group and combination group. Rat models of cerebral palsy were prepared in al groups except the control group. At 7 days after modeling, in the transplantation group, 10μL placenta derived mesenchymal stem cel s were transplanted into the right cerebral cortex and striatum of rats;rats in theacupuncture group were given acupuncture at Shuigou (DU26), Dazhui (DU14), Baihui (DU20), Guanyuan (RN4) and Qihai (RN6) points;rats in the combination group given acupuncture and transplantation treatment as described above;the model and control groups received no treatment. Then, after 7-day intervention, the body mass, neurological function and pathological changes of rat brain tissues were measured and observed, respectively. . RESULTS AND CONCLUSION:The order of the growth rate of body mass was as fol ows:control group>combination group>acupuncture and transplantation groups>model group, and al groups had significant differences except the two groups of acupuncture and transplantation (P<0.05);findings from holding tract, foot error and water finding in maze tests showed the neurological function was best in the control group, fol owed by the combination group, as wel as acupuncture and transplantation groups, and the worst in the model group. Al groups had significant differences except acupuncture and transplantation groups (P<0.05). Besides, in the model group, cel s in the brain and hippocampus decreased, arranging disorderly with fuzzy structure, and inflammatory cel s appeared;pathological changes in the other groups were in between control group and model group, Additional y, brain tissues in the combination group exlibited better pathological changes than those in the acupuncture and transplantation groups, which were similar with the normal brain tissues. To conclude, acupuncture, placenta derived mesenchymal stem cel s transplantation, or their combination can improve neurological function in rats with cerebral palsy, especial y the combination treatment.

BACKGROUND:Acupuncture is proved to have therapeutic effect on cerebral palsy of immature rats.
OBJECTIVE:To discuss the possible effect on cerebral amino acids and neurotransmitters in immature rats with cerebral palsy by using fire-needle.
METHODS:Cerebral palsy models were established in 45 Wistar rats aged 1 week by left common carotid artery ligation and randomly assigned into model, acupuncture and positive groups (n=15 per group). The other five rats were defined as controls, without any management. At 3 days, positive group rats were oral y administrated with brain enzyme solution, and acupuncture group given fire-needle treatment at points of Neiguan (P 6), Yongquan (K 1), Quchi (LI 11) and Baihui (Du 20) for 21 days. Hanging test, inclined plane test and Y maze test were performed 1 hour after the last treatment, and the cerebral cortex and hippocampus were extracted and homogenized after execution. Lelves of acethl choline, norepinephrine, 5-hydroxytryptamine, glutamic acid andγ-aminobutyric acid were recruited, and morphology of hippocampus was observed by pathological section.
RESULTS AND CONCLUSION:(1) Within 21-day treatment, positive and acupuncture treatment rats were more active than model group. Compared with the control group, the body mass in the model group was significantly decreased (P<0.05);compared with the model group, the body mass in the positive and fire-needle groups were significantly increased (P<0.05), while it showed no significant difference between latter two groups. (2) The hanging time of model group rats was significantly shorter than that of control group (P<0.05), and significantly lengthened in the positive and fire-needle groups compared with the model group (P<0.05). Compared with the control rats, the error numbers of model group rats were significantly increased (P<0.05), and conversely, significantly decreased in the positive and fire-needle groups (P<0.05). (3) In the model group, the levels of norepinephrine, 5-hydroxytryptamine andγ-aminobutyric acid were significantly decreased, while glutamic acid significantly increased compared with the control group (P<0.05);compare with the model group, the positive and fire-needle group rats held significantly increased levels of norepinephrine, 5-hydroxytryptamine andγ-aminobutyric acid and decrease of glutamic acid (P<0.05). (4) Hematoxylin-eosin staining showed that in the mode group, there were abundant neurons damaged, cel volume reduced, nucleus pycnosis and unclear border with nucleus;both positive and fire-needle groups could improve neuron structure induced by cerebral ischemia. (5) In conclusion, fire-needle can treat cerebral palsy effectively.

Objective To compare safety and feasibility using radial versus femoral access during cardiac catheterization of patients who had previously undergone coronary artery bypass graft ( CABG) surgery. Methods We retrospectively evaluated 116 consecutive patients who underwent graft intervention via the transradial (TRA group, n = 46) or transfemoral approach (TFA group, n = 70), and observed the baseline clinical characteristics, angiography characteristics and complications between the 2 groups. Results The baseline clinical characteristics between the 2 groups were similar ( all P > 0. 05) . No significant difference was observed in angiography characteristics and procedural parameters including operation time, radiation exposure and puncture time between the 2 groups (all P > 0. 05). There was no significant difference in major adverse cardiac events during hospitalization. PCI to graft vessels were all successful and procedural success rates were similar between the 2 groups (P = 0. 669). Vascular access site complications were significantly lower ( P = 0. 03) in the TRA group. No access site complication was recorded in the TRA group. 7 cases (10. 0% ) with complications were recorded in the TFA group including 1 case of major bleeding (1. 4% ), 3 cases of minor bleeding (4. 3% ), 2 cases of local hematorna (2. 9% ) and 1 case of A-V fistula formation. Conclusions In contrast to the transfemoral route, the rate of major vascular complications was negligible using the transradial approach.

Objective To investigate the method and value of adjustable interatrial fistulization in the operation of congenital heart disease(CHD) accompany with severe pulmonary arterial hypertension(PH).Methods Twenty-seven patients(19 male,8 females) accompany with severe PH were entered the study,age ranged from 4 to 14 years old,weight from 13.7 to 42.0 kilogram.The enrolled diseases included 11 cases of atrial septal defect(ASD),10 cases of ventricular septal defect(VSD),4 cases of patent ductus arteriosus(PDA),and 2 cases of Ebstein syndrome accompany with severe tricuspid insufficiency.All patients were diagnosed as CHD accompany with severe PH(bidirectional shunt)which was the contraindications for routine operation before operation through chest X-ray,electrocardiography,ultrasonic cardiography,cardiac catheteri-zation and cardiac angiography.Results With adjustable interatrial fistulization and treatment to the abnormalities,14 fistulaes were closed immediately after operation,7 fistulaes were closed 2 days after operation,3 fistulaes were closed 3 days and 1 fistulae was closed 4 days after operation and accompanied with empyema discharged initiatively.One fistula was never closed,1 case died from low cardiac output symptom.The effective rate was 92.6%,closed to that of routine operations.Conclusion Adjustable interatrial fistulization is an easy procedure,and it can decrease the danger of PH post-operation effectively and provide operation opportunity for those patients with CHD approaching terminal stage.

OBJECTIVETo investigate the effect of hypoxia on the human pulmonary artery smooth muscle cells two pore domain potassium channels TASK-1 and the regulation of non-receptor tyrosine kinase c-Src in this process.

METHODSThe cultured human pulmonary artery smooth muscle cells (hPASMCs) were divided into: normal group, hypoxia 30 minute group, hypoxia 6 hours group and hypoxia 48 hour group, and hypoxia 48 hour + PP2 group, hypoxia 48 hour + PP3 group, hypoxia 48 hour + bpV group. Flow cytometry was used to analyze the cell cycle, RT-PCR and Western blot technique were carried out to detect the expression changes of TASK-1 mRNA and protein in different groups.

RESULTS(1) Cell Cycle Show: Compared with normal control group, with prolonged hypoxia, the percentages of hPASMCs in S phases of cell cycle were increased. While compared with hypoxia 48 hour group, the percentages of hypoxia 48 hour + PP2 group hPASMCs in S phases of cell cycle were decreased. The expression of TASK-1 mRNA on hPASMCs in acute hypoxia 6 hour group was increased, while the expression of TASK-1 protein on hPASMCs in the acute and chronic hypoxia group was decreased, and the expression of TASK-1 mRNA on hPASMCs in the chronic hypoxia group was decreased; After pre-incubation of a potent and selective inhibitor of the Src family of protein tyrosine kinases PP2, the expression of TASK-1 mRNA and protein in hypoxia 48 hour group was increased, however after pre-incubation of the inhibitor of the Src family of protein tyrosine phosphatase bpV, the expression of TASK-1 protein in hypoxia 48 hour group was decreased.

CONCLUSIONHypoxia promotes human pulmonary artery smooth muscle cell proliferation, and non-receptor tyrosine kinase c-Src may participate in the expression of two pore domain potassium channels TASK-1 regulated by hypoxia. Therefore, we hypothesized that TASK-1 channels and c-Src participatein the acute and chronic hypoxic human pulmonary vasoconstriction.

Cell Hypoxia ; Cell Proliferation ; Cells, Cultured ; Humans ; Myocytes, Smooth Muscle ; cytology ; Nerve Tissue Proteins ; metabolism ; Potassium Channels, Tandem Pore Domain ; metabolism ; Pulmonary Artery ; cytology ; RNA, Messenger ; Vasoconstriction ; src-Family Kinases ; metabolism

Adult ; Antineoplastic Agents ; adverse effects ; Chromosome Aberrations ; chemically induced ; DNA Damage ; Female ; Humans ; Micronucleus Tests ; Nursing Staff, Hospital ; Occupational Exposure

Objective:To investigate the effect of bone marrow mesenchymal stem cells transplantation on the cognitive function in hippocampus after traumatic brain injury in rats.Methods: BMSCs were isolated and cultured from male 3-week-old SD rats in vitro.60 female SD rats were randomly divided into 3 groups(n=20),control group,model group,BMSCs treatment group.After the es-tablishment of brain trauma model of the model 1,7,14,28 d,BMSCs treatment group was given BMSCs(1×106ml-1)intervention.In addition,20 rats were tested by water maze,and each group was divided into four points:6,7,8 9 d after injury.The morphological changes of brain tissue was detected by HE staining;the water content of brain tissue was detected by dry wet method;Morris detection method,detection of spatial learning and memory ability in rats;nerve motor function score, neural function analysis detection;field test,detection of spatial learning and memory ability in rats.Results: The morphological changes of brain were observed by HE staining;the water content of brain tissue was detected by wet and dry gravity;the water maze test was conducted to examine the spatial learning ability,the motor function score,and the field experiment.The results of wet and dry gravity test showed that compared with the control group,the model group was significantly higher than the control group(P< 0.05),the water content of the treatment group at each time was significantly lower than the model group;the results of water maze test showed that the latency of safe island was significantly increased in the model group on the eighth and ninth day after injury,compared with the control group,and the difference was statistically significant(P<0.05).In the treatment group,the safe island latency was significantly shorter in the eighth day and ninth day after injury than in the control group;the neurological score results showed that compared with control group,model group,rats in the hind limb grasping reflex,vibrissae evoked forelimb positioning and lateral pad step tests were not normal performance,resulting in functional score decreased with statistical significance(P<0.05).The functional scores of the treatment group and the model group were significantly higher than those of the model group.Conclusion:Through the behavioral related tests,the data show that BMSCs can restore the neurological function to a certain extent after traumatic brain injury in rats.

BACKGROUND:Meta-analysis results have shown that Duhuojisheng Decoction can improve the symptoms and relieve the pain of patients with knee osteoarthritis, but the mechanism and pathway are not very clear.OBJECTIVE:To further verify the curative effect of Duhuojisheng Decoction for primary knee osteoarthritis in macaca fascicularis. METHODS: Under natural conditions, 6 clean young macaca fascicularis (aged 3-5 years, normal group) and 6 elderly macaca fascicularis (aged more than 20 years, model group) were selected from 24 macaca fascicularis. The models of primary knee osteoarthritis were established in the model group,then aged animals were randomly divided into control and intervention(given the treatment of Duhuojisheng Decoction) groups. The number of whole blood leukocytes, C-reactive protein, erythrocyte sedimentation rate, properties of the joint fluid and expression level of tumor necrosis factor-α were compared between the control and intervention groups. RESULTS AND CONCLUSION: The aged macaca fascicularis was characterized by a typical osteoarthritis similar to human. Treatment with Duhuojisheng Decoction significantly down-regulated the expression level of tumor necrosis factor-α and significantly reduced the levels of whole blood leukocytes, and C-reactive protein. Our results suggest that the aged macaca fascicularis model of primary knee osteoarthritis is an effective animal model to simulate the occurrence and development of human primary knee osteoarthritis.Duhuojisheng decoction alleviates the progression of primary knee osteoarthritis probably by inhibiting the expression level of tumor necrosis factor-α and decreasing the levels of inflammatory factors in the articular cartilage. Additionally, the levels of whole blood leukocytes and C-reactive protein are important parameters for the prediction of curative effects.