1.Influence of quercetin and bornyl acetate on ratio of IFN-γ/IL-4 and macrophages in uteri of LPS-induced abortion mice
Xiaodan WANG ; Aituan MA ; Wanyu SHI ; Guojun JIANG ; Xiuhui ZHONG
Acta Anatomica Sinica 2009;40(4):637-641
Objective To investigate significance of interferon-γ/interleukin-4(IFN-γ/IL-4) and macrophages in uterus in early embryo loss (or resorption), and to elucidate the anti-abortive effect and the immunological modulation at maternal-fetal interface with quercetin and bornvl acetate. Methods Lipopolysaccharide (LPS)(0.10μg/mouse)was injected via tail vein in order to induce abortion in 7-day-gestation mice which received quercetin and bornvl acetate at days 4-7 of gestation. Levels of IFN-γ and IL-4 in uterus lysate supernatant were measured using enzyme-linked immuno-absorbent assay (ELISA), and uterine macrophages of each group ( n =10) were detected by immunohistochemistry. Results The amount of macrophages was much higher in the uteri of LPS-induced abortion mice than that in control mice. The abortion rate of mouse declined to certain level. The therapy of quercetin combined with bornyl acetate reversed LPS-induced abortion. Conclusion The increase of IFN-γ/IL-4 and the amount of macrophages in the LPS-treated mouse uterus is associated with the embryo loss, and quercetin and bornyl acetate have the anti-abortive effect through modulation of maternal-fetal interface immunity balance.
2.The application of implantable venous access ports in management of postoperative breast cancer patients
Zhimin LIU ; Yuan ZHONG ; Xueqing JIANG ; Pengfei SHI ; Yang LI
Chinese Journal of Endocrine Surgery 2016;10(2):148-151
Objective To investigate and analyze the experience of planting and maintaining implantable venous access ports(IVAP) in management of post-operative breast cancer patients. Methods Breast cancer pa-tients receiving IVAP after surgery from Mar. 2011 to Jun. 2014 were retrospectively analyzed. The relative com-plications were documented and summarized during implanting operation. Results 468 patients received IVAP, among whom 451 patients underwent piercing implantation via right internal jugular vein, 15 patients underwent piercing implantation via right subclavian vein, and 2 patients underwent piercing implantation via left internal jugular vein. The mean cathe tering leng th was 12.8 cm for patients receiving IVAP via right internal jugular vein, ranging from 12 to 15 cm. Thereinto, 30(6.4%) patients experienced shot-term complications including 16 cases of puncture difficulty, 5 cases of accidental arterial puncture, 2 cases of extravasation, 2 cases of blood aspiration dif-ficulty and 5 cases of arrhythmia. Three cases had long-term complications as the following:one case of catheter-re-lated infection, one case of catheter lost, and one case of incision rupture. Conclusions IVAP is a safe and effec-tive intravenous infusion device. It is crucial to choose individualized implanting access and length by professional surgical team.
3.Clinical analysis of seven acute phosphine poisoning.
Tao CHEN ; Ran SHI ; Xue-zhong YANG ; Xue-zhong YANG ; Ming-jiang QIAN ; Hua-jun CHEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(3):223-225
Acute Disease
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Adult
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Humans
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Male
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Occupational Diseases
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diagnosis
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therapy
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Phosphines
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poisoning
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Poisoning
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diagnosis
;
therapy
4.Multiple lymphomatous polyposis of intestine: report of a case.
Cai-qin WANG ; Zhong-xin SHI ; Jing JIANG ; Ji-hong ZHANG ; Ying ZHANG ; Qian WANG
Chinese Journal of Pathology 2011;40(5):341-342
Antigens, CD20
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metabolism
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CD5 Antigens
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metabolism
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Colonic Neoplasms
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complications
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metabolism
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pathology
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surgery
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Cyclin D1
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metabolism
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Diagnosis, Differential
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Female
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Humans
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Ileal Diseases
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complications
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pathology
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surgery
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Ileocecal Valve
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Intestinal Neoplasms
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complications
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metabolism
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pathology
;
surgery
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Intestinal Polyps
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complications
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metabolism
;
pathology
;
surgery
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Intussusception
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complications
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pathology
;
surgery
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Leukemia, Lymphocytic, Chronic, B-Cell
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metabolism
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pathology
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Lymphoma, Mantle-Cell
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complications
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metabolism
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pathology
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surgery
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Middle Aged
5.Significance of peripheral blood T lymphocyte subsets and NK cells detection in lung cancer diagnosis and treatment
Jinhua JIANG ; Tinghua YAN ; Suiwan LU ; Guowei ZHONG ; Yongxin XIE ; Xiang CHEN ; Yanfen SHI ; Zhifeng ZHOU
Cancer Research and Clinic 2013;(2):90-93
Objective To analyse the relationship between T lymphocyte subsets and NK cells expression and dynamic changes in lung cancer patients 'peripheral blood and the occurrence and development of cancer,and investigate their clinical significances.Methods Flow cytometry was applied to detect 66 patients with lung cancer,60 patients with pulmonary tuberculosis and 60 healthy persons peripheral blood CD+3,CD+3CD+8,CD+3CD+4,Th/Ts,CD+16CD+56 expression.Lung cancer group peripheral blood CD+3,CD+3CD+8,CD+3CD+4,Th/Ts,CD+16CD+56 expression were also detected on 3rd,7th and 20th day before and after chemotherapy.Results Lung cancer group CD+3,CD+3CD+4,Th/Ts,CD+16CD+56 expression decreased significantly [(54.23±10.37)%,(34.23±8.03)%,1.35±0.20,(25.18±4.34)%] and had significant differences compared with pulmonary tuberculosis group [(63.09±9.19)%,(39.46±12.74)%,1.51±0.41,(26.45±3.96)%] and healthy group [(69.68±8.31)%,(42.31±13.29)%,1.89±0.48,(29.44±2.51)%](P < 0.05),but CD+3CD+8 expression showed no significant difference(P > 0.05).In chemotherapy group,comparing with before chemotherapy,remission group CD+3,CD+3CD+4,Th/Ts and CD+16CD+56 expression decreased significantly (P < 0.01)on 3rd day after chemotherapy,while CD+3CD+8expression increased significantly(P < 0.01).On 7th day,each index recovered to the level of before chemotherapy basically.On 20th day,CD+3,CD+3CD+4,Th/Ts and CD+16CD+56 expression increased significantly(P < 0.05)compared with before chemotherapy,while CD+3CD+8 expression significantly decreased(P < 0.05).Chemotherapy unease group had no significant difference (P > 0.05).Lung cancer of stage Ⅲ A and Ⅲ B compared with stage Ⅰ A,and lymph node metastasis in N3 group compared with N0 group,CD+3,CD+3CD+4,CD+3CD+8,Th/Ts and CD+16CD+56 expression had significant differences (P < 0.05).Compared with their pathological types,each index had no significant difference(P > 0.05).Conclusion Monitoring the peripheral blood T lymphocyte subsets and NK cells dynamic of lung cancer patients can guide the clinical diagnosis and treatment,and contribute to the assessment of immune function.
6.The diagnosis and treatment of the sharp injury in the back.
Shi CHENG ; Zhiqiang ZHONG ; Ruotian WANG ; Yiezhi ZHAO ; Zhihong LI ; Ming JIANG ; Huisheng YUAN ; Dongbo FAN
Chinese Journal of Practical Surgery 2001;21(4):216-217
Objective To improve the level of diagnosis and treatment of the sharp injury in the back. Methods 47 cases which were treated from Jan 1991 to May 2000 were reviewed. ResultsAmong 37 cases who underwent the exploration, 5 cases died. Among 10 cases who underwent conservative treatment, 2 cases died. ConclusionThe condition of sharp injury in the back is very complicated,it is easy to be misdiagnosed, the mortality is high. Saving should be equalled with the diagnosis and treatment. The application of wound exploration, abdominal puncture, ultrasound examination and X-ray checking is valuable to the diagnosis. The patients with operation indications should be operated at once,while the others should be observed for some time to prevent the delayed clinical manifestation.
7.The blood homocysteine level and distribution analysis of Ganzhou city people
Lixia JIANG ; Shaohua SHI ; Xingming ZHONG ; Yihong YANG ; Rong HU ; Xuxiang XI
Chongqing Medicine 2014;(31):4197-4199,4202
Objective We analysed the homocysteine level and distribution of ganzhou city crowd ,learned about its elevatory sit‐uation ,and provided theory basis for the intervention of high homocysteine level crowd and the prevention of heart and head blood vessel disease .Methods We detected the blood homocysteine level of 6419 adults who had physical examination in our hospital dur‐ing 2012 by enzymatic methods ,and analyzed the diagnose rate of HHcy and the distributions of Hcy in different age and gender . Results The total detection rate of high homocysteine level in 6419 adults was 39 .11% ,among which 47 .30% in male and 22 .49% in female ,the comparison was statistically significant (χ2 = 424 .28 ,P< 0 .05) ;the detection rate of male was higher than female .In different ages ,the detection rates were statistically significant comparatively (P< 0 .05) .The detection rate of homocys‐teine level in both gender rises with age .At the same time ,homocysteine levels in males of 25 - 34 years old age group and 35 - 44 years old age group ,45 - 54 years old age group and 55 - 64 yeas old age group were not statistically significant ;while there were statistical differences of Hcy level in the rest age groups of male ,different age groups of female and same age different gender groups (P< 0 .05) .Conclusion The HHcy detection rate and the blood Hcy distribution of Ganzhou city crowd has its regional characteristics compared with other regions ,and it may be associated with the main population distribution of hakka people and oth‐er geological factors .The detection rate of HHcy was relatively high ,and it′s advisable to take early prevention to lower the risk of heart and head blood vessel disease .
8.The purification of anti-D antibody from IgG contained anti-D by the RhD positive red blood cell
Mingjun SHI ; Jing XIE ; Jiang ZHONG ; Huazhen GUI ; Bing GUO ; Guozhong ZHANG
Chinese Journal of Immunology 2000;0(11):-
Objective:To investigate the purified methods of human anti-D antibody from IgG contained anti-D. Methods:The IgG was separated by the column ion-exchange chromatography(CIEC) from the plasma in which the content of anti-D was 0.814 ?g/ml. Then the IgG preparation contained anti-D was purified by the affinity chromatography(AC) with the O group, RhD positive red blood cell (genotype CCDee). Results:The content of non anti-D IgG were reduced about 90% by the method of AC and the proportion of anti-D could be significantly increased in the final preperation. The quality of final preparation attained reqirements of national standard of biologics. Conclusion:This method is able to purify anti-D from IgG contained anti-D and offer a reference for plasma products.
9.Influence of Angelica Sinenisis Injection on Bone Marrow Histology and Ultrastructure in Immune-Induced Aplastic Anemia Mice
qing-zhao, SHI ; zhi-liang, XU ; hui, WANG ; zhong-yan, JIANG
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To investigate the influence of angelica sinenisis injection on bone marrow histology and ultrastructure in immune-induced aplastic anemia(AA) mice.Methods Female Balb/c mice were divided into normal group,model group,therapy group and contrast group.All mice were killed by cutting neck on the 12 th day, ulnas and femur were taken out. The bone marrow histology section and ultrastructure of mice ulnas were observed. The quantities of hematopoietic cells were counted. The number of femur bone marrow mononucleate cells (MNC) were measured.Results The quantities of hematopoietic cells in model group were lower than those in normal group (P
10.Research status, development and utilization of traditional Dai medicine in China.
Bao-Zhong DUAN ; Jiang XU ; Hai-Tao LI ; Chen SHI-LIN
China Journal of Chinese Materia Medica 2015;40(1):18-23
OBJECTIVETo provide a reference for the development and utilization of Dai medicine by investigate the present situation and existing problems of traditional Dai medicine.
METHODCombined with the previous relevant investigations and literature in the field, the key and the development direction of traditional Dai medicine were analyzed.
RESULTThe textual research, history, species, distribution, endangered resources, protection status etc. were elaborated and the key strategy of further investigation was expounded.
CONCLUSIONDai medicine resources should strengthen the basic research, such as the protection of traditional knowledge, the textual research, quality standard, chemical composition, biological activity, exploration of medicinal resources, especially monographic study on protection of major endangered medicinal resources should be intensified, which will be rise the level of development and utilization of Dai medicine resources.
China ; ethnology ; Conservation of Natural Resources ; history ; Drugs, Chinese Herbal ; history ; pharmacology ; History, 20th Century ; History, Ancient ; Medicine, Chinese Traditional ; history ; Plants, Medicinal ; growth & development