Actins ; metabolism ; Adult ; Diagnosis, Differential ; Endometrial Neoplasms ; metabolism ; pathology ; surgery ; Endometrial Stromal Tumors ; metabolism ; pathology ; surgery ; Female ; Humans ; Hysterectomy ; Leiomyoma, Epithelioid ; metabolism ; pathology ; Melanoma-Specific Antigens ; metabolism ; Perivascular Epithelioid Cell Neoplasms ; metabolism ; pathology ; surgery ; Receptors, Progesterone ; metabolism ; Sarcoma, Clear Cell ; metabolism ; pathology ; Uterine Neoplasms ; metabolism ; pathology ; surgery

Objectiv To evaluate the usefulness of dot immuno-gold filtration assay(DIGFA) for the diagnosis of Paragonimus infection.Methods During 2003 to 2006,72 cases suspected of paragonimiasis in Zhejiang Province were examined with DIGFA for rapid detection of specific antibodies against Paragonimus(Pw-DIGFA).The diagnosis was primarily established with the presence of antibodies,experience of ingesting raw freshwater crabs or crayfishes and clinical presentations.The cases were treated with praziquantel and followed-up at 3 and/or 6 months post-treatment.Antibody level in patients(pre-and post-treatment) were detected in parallel and analyzed comparatively by Pw-DIGFA and ELISA.Results The result of detection by Pw-DIGFA was in agreement with that of ELISA.28 of 72 cases were antibody positive and 44 cases were negative.Among the 28 positives,26 cases had a history of eating raw freshwater crab or crayfishes and the other 2 cases drank freshwater from brook before.21 cases showed paragonimiasis-related clinical symptoms such as low-grade fever,cough,or changes in image examination,while the other 7 cases showed only eosinophilia in peripheral blood(15%-70%).The mean absorbance values(A450) of positive sera,negative sera and normal sera tested by ELISA were 1.7361,0.2973 and 0.2657 respectively.There was significant difference between the positive cases and the negative cases(t=12.047,P0.05).At 3 month post-treatment,serum antibody in 5 cases whose clinical symptoms and physical signs relieved or disappeared decreased 2-5 titers and that of one case who relapsed with new signs increased by one titer.In Pw-DIGFA,the dot color of 5 cured cases showed a little weaker than that of pre-treatment and the relapsed case displayed similar response.At 6 month post-treatment,7 sera of clinically cured cases showed significantly weaker response than that of pre-treatment.The antibodies of those sera dropped 3-6 titers.Conclusion Pw-DIGFA is of supplementary value for clinical diagnosis of paragonimiasis.Antibody detection by pre-and post-treatment using Pw-DIGFA shows potential for the evaluation of therapeutic effect.

Food Sanitation Law which was issued for many years has no longer been congruent with the current development of socio-economy. Under this situation,Food Safety Law came into being. Although the promulgation of the law is important,the task of carrying out the law is more difficult. On the basis of concluding the problems in carrying out the food sanitation law and the analysis of its causes,it is significant to discuss the Food Safety Law from the viewpoint of Food Sanitation Law enforcement.

Objective To investigate effects of Ginkgo biloba extract(EGb) on expression of CREB and pCREB in cortex of aging rats.Methods Male Wistar rats were divided into three groups:young control group,old control group and EGb group.Rats in EGb group were treated with intragastric administration of EGb,while rats in the other two groups were treated with distilled water.The spatial learning and memory were evaluated by Morris water maze,and the expression of CREB,pCREB were detected by western blot.Results( 1 ) Compared with young control group (9.6 ± 2.88,41.55 ± 6.30),the swimming time and times through platform in the target quardrant of rats in old control group(6.8 ± 2.49,34.92 ± 4.56) were reduced (P ＜ 0.05 ).The times passing through the platform and the time exploring the target quadrant were more and longer in EGb group(9.4 ± 2.63,41.0 ± 6.68 ) than those in old control group(P ＜ 0.05 ).(2)Compared with rats in young control group( 1.07 ±0.33,0.26 ± 0.04),relative contents of CREB and p-CREB proteins in cortex (0.70 ± 0.21,0.13 ± 0.05 ) weredecreased in old control group(P＜0.05 ).CREB and p-CREB Levels were higher in EGb group ( 1.02 ±0.18,0.18 ±0.02)than those in old control group(P ＜ 0.05 ).Conclusion EGb can ameliorate spatial learning and memory of rats by increasing the expression of CREB and p-CREB in cortex.

In order to clarify the chemical composition and source of Banxia Xiexin decoction quickly and comprehensively, whole and individual herbs of Banxia Xiexin decoction were analyzed by ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS(E)). Under identical experiment conditions, chromatography results were compared between experiment groups. Based on the Q-TOF-MS(E) analysis, 74 peaks were identified on line. The herbal sources of these peaks were assigned. The results implied that flavonoids, triterpenoid saponins, alkaloids and glycosides were the main components in effective part of Banxia Xiexin decoction. The method established is simple and rapid for elucidation the constituents of Banxia Xiexin decoction and the results could be used for the quality control of Banxia Xiexin decoction.

Humans ; Occupational Health ; Refuse Disposal

BACKGROUND:Core decompression and autogenous bone grafts are widely used in treatment of early avascular necrosis of femoral head. According to the report, the success rate of this therapy has obvious difference;the reasons may be related to inaccurate puncture location and secondary damnification of repeated puncture. OBJECTIVE:To reconstruct three-dimensional model of femoral head necrosis by Mimics software for reappearance of lesions in the necrotic area to realize measurement of necrotic area of the femoral head and estimation of its volume. METHODS:We restructured images by using multi-slice spiral CT Syngommvvp VE23A workstation, Inspace software and NeuroDSA software. Hip CT data in DICOM format were imported into Mimics 13.0 software systems. Necrotic area of the femoral head was reconstructed with Mimics SimuIation software to truly reproduce the integrated form, scope and stereochemical structure of the necrotic area so as to achieve the measurement of the necrotic area of the femoral head and the volume estimation. We designed the best core decompression channel, simulated core decompression surgery, so that the patients could refer to the best simulated decompression path in the operation of core decompression. RESULTS AND CONCLUSION:(1) Among 36 patients (48 hips) with avascular necrosis of femoral head, there were phase I in 8 hips, accounting for 17%, phase II in 28 hips, accounting for 58%, and phase III in 12 hips, accounting for 25%. (2) The volume of necrotic area was (1 475.48±647.342) mm3 in the phase I, (4 571.77±2 344.55) mm3 in the phase II, and (4 836.46±2 969.33) mm3 in the phase III. (3) We simulated the core decompression based on the radius of the sphere of the necrotic area as parameter in the Mimics Simulation software module, and then completely cleared the necrotic area. (4) Surgery can more clearly understand information and stereochemical structure of the necrotic area with Mimics software to simulate the core decompression. It is the theoretical basis of operation.

0.05). Among 36 sera collected at 12 months post-treatment, the antibody negative conversion rates were 80.6% (29/36) and 58.3% (21/36), there was a significant difference between the two assays (P

OBJECTIVE To investigate spectrum and drug resistance of No-Pseudomonas aeruginosa,to provide the basis for proper use of antibiotics and prevent patients from hospital acquired infection.METHODS API staph analysis system and the K-B agar diffusion test according to NCCLS were used to identify 357 strains of No-Pseudomonas aeruginosa and to performe drug sensitivity test.RESULTS From 2004 to 2008,357 strains of No-Pseudomonas aeruginosa were isolated.There are 233 Stenotrophomonas maltophilia strains(65.27%,233/357),55 Burkholderia cepacia strains(15.41%,55/357).Sputum was the main sourse of No-P.aeruginosa(88.52%,316/357).The isolation rate of No-P.aeruginosa was the highest in intensive care units(38.10%,136/357),the next was department of respiratorymedicine(23.53%,84/357).The results of drug susceptibility test showed that the resistance rate of No-P.aeruginosa to most drugs were higher,only the resistance rates of S.maltophilia to minocycline,gatifloxacin and levofloxacin were lower,(3.26%,20.55% and 20.83% respectively),The resistance rates of B.cepacia to meropenem,piperacillin/tazobactam and Imipenem were lower,being 29.17%,33.33% and 39.62% respectively.CONCLUSIONS The most common strains is S.maltophilia and Burkholderia cepacia,the most common site of infection is lower respiratory tract,Most strains of S.maltophilia are isolated from ICU and department of respiratory diseases.The result of drug sensitive test showed that there were high rates of multiple drug resistance in No-P.aeruginosa and treatment of No-P.aeruginosa infection should be based on the results of the drug susceptibility test.

OBJECTIVE To investigate hand hygiene of healthcare workers in burn ward in our hospital and provide scientific basis for the effective prevention and control of hospital infection in burn ward.METHODS Selected randomly 30 healthcare workers in burn ward and sampled from hands for bacterial culture.RESULTS The average colony of hand pathogens of the 30 healthcare workers was 105.42 CFU/cm2,the maximam was 1093 CFU/cm2.Among the hand pathogens,40.91% were Bacillus subtilis,36.36% were micrococcus and 13.64% were Staphylococcus aureus.CONCLUSIONS Hand hygiene of healthcare workers are not optimistic in burn ward.Strengthening the education of hand hygiene and raising the executable rate of hand hygiene are important measures to prevent and control the hospital infection in burn ward.