Objective To evaluate the clinical result of modified anterior decompression for the treatment of cervical myelopathy due to ossification of posterior longitudinal ligament(OPLL). Methods From April 1994 to October 2002, 42 cases of OPLL involving 134 cervical segments were operated using modified anterior decompression, among them there were 35 cases, 122 segments of the continuous type; 4 cases, 9 segments of the mixed type; 3 cases, 3 segments of the solitary type. The preoperative index of thickness (O-Index) ranged from 12.4%-78.8%, with an average of 43.2%. A slot was made in the vertebral body through anterior approach and bilateral border of the ossification was dissected from the posterior wall of vertebral body with burr-drill. Results The average follow-up was 3 years and 8 months ranging from 1 year and 6 months to 8 years. The recovery of neurologic function was evaluated according to JOA score: 28 cases with mild neurologic deficit (13-16 points) improved on average 74% postoperatively; 11 cases of the moderately damaged group (8-12 points) improved on 75%; 3 cases of the severely damaged group (

OBJECTIVE: Because of the widely origin, easily isolation and cultivation, the powerful multiplication ability and some other characteristics, endothelial progenitor cells have become the most important seeding cells in tissue-engineered vascular, but the condition to become a vascular in vitro, characteristics of endothelial progenitor cells and their progress in research and application in the tissue-engineered vascular deserve further study. DATA SOURCES: A computer-based online search of PUBMED database was undertaken to identify articles about endothelial progenitor cells being seeding cells in tissue-engineered vascular application published from January 1997 to July 2006 by using the keywords of "Endothelial progenitor cells,Tissue-engineered vascular,Tissue Engineering Blood Vessel" in English. STUDY SELECTION: The data were selected firstly, and quotation of each articles were read. Inclusive criteria: all articles related to the characteristics of endothelial progenitor cells and their progress in research and application in the tissue-engineered vascular were selected. Exclusive criteria: repetitive research or Meta articles were excluded. DATA EXTRACTION:Totally 136 articles were collected, of which 30 articles were associated with inclusive criteria. The 106 excluded articles were with old content or repetitive research. The 30 articles contained 8 articles on characteristics of endothelial progenitor cells, 14 articles about the advantages of endothelial progenitor cells as seeding cells in tissue-engineered vascular application, 8 articles on the application of endothelial progenitor cells construction in tissue-engineered vascular. DATA SYNTHESIS: ① Endothelial progenitor cells could be isolated from bone-marrow, peripheral blood, spleen and so on. They expressed CD133 and distinction from endothelial cells by CD133. Endothelial progenitor cells could transform to endothelial cells and smooth muscles, too. ② Endothelial progenitor cells could raise the density of capillary and the number of angiovascular. There were many research indicated that endothelial progenitor cells could improve ischemic, repair the injure of endocardium; Endothelial progenitor cells could improve reendothelialization, protect endomembrane, and hold the integrity of endomembrane; when incorporated with ischemic vascular, endothelial progenitor cells could improve patency rate and prevent restenosis. ③ With smooth muscle cells and some other materials, endothelial progenitor cells could widely applied in constructing tissue-engineered vascular and have a brilliant prospect in the tissue-engineering blood vessel. CONCLUSION: The research of endothelial progenitor cells will have a breakthrough in the expression of its vessel and the function in the organization remodeling. As one of the seeding cells, endothelial progenitor cells have widely origin and the powerful multiplication ability, and they have a brilliant prospect in the tissue-engineering blood vessel.

Objective: By observing the differences in Western Ontario and McMaster Universities osteoarthritis index (WOMAC) and visual analog scale (VAS) scores in moxibustion treatment for moderate-to-severe primary knee osteoarthritis (KOA) with moxa of different storage years (3-year moxa and 1-year moxa from Qichun, Huanggang City, Hubei Province, China) through a randomized clinical trial, to objectively evaluate the differences in therapeutic efficacy of moxibustion with moxa of different storage years. Methods: A total of 63 patients with moderate-to-severe KOA who met the inclusion criteria were randomized into moxibustion group 1 and moxibustion group 2 by central randomization method, with 32 cases in moxibustion group 1 and 31 cases in moxibustion group 2. Moxibustion group 1 was treated with moxa stored for 3 years, and moxibustion group 2 was treated with moxa stored for 1 year. Dubi (ST 35), Neixiyan (EX-LE 4) and Heding (EX-LE 2) were selected in both groups, and the treatment lasted 20 min per time, 3 times a week. The immediate efficacy was compared after 6 times of treatment, and long-term efficacy was compared at follow-up 4 weeks after the end of treatment. Results: During the treatment, there were 2 dropouts in moxibustion group 1, and 1 dropout in moxibustion group 2. The total effective rate in the two groups was 83.3％ and 60.0％, respectively. Followed up at 4 weeks after the end of treatment, the total effective rate in the two groups was 80.0％ and 66.7％, respectively. There were no statistical differences between the two groups (both P>0.05). After treatment and 4 weeks after the end of treatment, the WOMAC and VAS scores in both groups decreased significantly compared with those before treatment (all P<0.01); the scores of stiffness item of WOMAC in moxibustion group 1 were lower than those in moxibustion group 2 (both P<0.05); there were no statistical differences in the scores of pain item and dysfunction item of WOMAC, and VAS scores between the two groups (all P>0.05). Conclusion: Moxibustion with moxa of different storage years (stored for 3 years and 1 year) both can improve the pain, stiffness and motor function in patients with moderate-to-severe KOA. While moxa stored for 3 years has a better therapeutic efficacy in improving stiffness of the knee joint than that stored for 1 year.

Aim To study the pharmacodynamics of antianxietic compound prescription capsule ( ACPC ) on acute stress in rats and the influence upon the ex-pression of ERK/CREB signal pathway and brain-de-rived neurotrophic factor ( BDNF) in the cerebral cor-tex and hippocampus of rats. Methods The elevated plus maze ( EPM ) test was applied to observe the effects of ACPC on acute stress rats administered 7 d low-, medium- and high-dose ( 0. 75 , 1. 5 , 3 g · kg-1 ) . The expression of ERK/CREB signal pathway and BDNF in the cerebral cortex and hippocampus of rats were studied by using Western blot method. Re-sults In EPM, high-dose of ACPC increased signifi-cantly the rat open arm time ( OT%) ( P<0 . 05 ) and the percentage of open arm entries ( OE%) ( P <0. 05). In Western blot, the medium-dose of ACPC reduced significantly p-ERK1/2 expression in hippo-campus ( P <0. 05 ) , and high-dose group decreased significantly the expression of p-ERK1/2 and p-CREB in the cortex and hippocampus of rats ( P <0. 05 ) . High-dose group increased significantly the expression of BDNF in the cortex and hippocampus of rats ( P<0. 05 , P<0. 01 ) . Conclusion ACPC has anti-anxie-ty effect in the model of EPM, and its mechanism may be related to the ERK/CREB signal pathway and in-creased BDNF expression.

Objective To investigate the recent studies about the relationship between Chlamydia pneumoniae and abdominal aortic aneurysm. Methods The current literatures about the relationship between Chlamydia pneumoniae and abdominal aortic aneurysm were reviewed. Results Chlamydia pneumoniae is one of the most important factors for the formation of abdominal aortic aneurysm since Chlamydia pneumoniae can cause abdominal aortic aneurysm through the metabolism of matrix metalloproteinases, the apoptosis of smooth muscle cells in the vessels and the chronic infection of the wall of the aneurysm. Conclusion There maybe a distinguishingly close relationship between Chlamydia pneumoniae and abdominal aortic aneurysm, and Chlamydia pneumoiae may take an important role in the development and progress of the abdominal aortic aneurysm.

Objective To investigate the effects of BKCa channel on electrophysiology excitatory regulation in MVN neuron following hypoxia and to reveal its molecular mechanism.Methods C57BL/6 mices were performed MVNs hypoxia mice model,and randomly allowed to normal oxgen group and hypoxia group.The hypoxia group, according to the application of NS1 6 1 9 ,was further divided into the no NS1 6 1 9 pretreatment group and NS1 6 1 9 pre-treatment group.Using the patch clamp experiment technology,we recorded the effects of the MVN abnormal neu-ronal firing and the change of the BKCa currents.Using immunohistochemical technique,the changes of BKCa in the hypoxic MVNs detected were.Results Acute hypoxia increased neuronal activities.NS1619 pretreatment de-creased hypoxia-induced firing rate,and increased and postponed the maximum increase by hypoxia(P<0.05),al-so alleviated 10-min-hypoxia-induced depolarization(P<0.05).Perfusion with hypoxic significantly reduced the BKCa positive neurons(P<0.05).Conclusion These findings suggest that acute hypoxia increases neuronal activi-ties.The decreased MVN BKCa channels contribute to hypoxia-induced abnormal neuronal activities.

Objective With the increasing of the CT examination, the dose of x-ray has been brought to public attention. For the sake of making the MDCT technology better applied, available optimal technology has to be used to reduce the x-ray dose to the patients. Methods Many optimal technologies were involved in, such as ECG modulation, cardiac bowtie, 3D dose modulation, compact geometry design, electron collector and speed 4D CARE dose. Results The result of the research showed that the dose of scanning x-ray of CT could be decreased sharply by using optimal technologies. The Q2 values was the standard which could well evaluate the quality of the CT′s images and the dose of x-ray. Conclusion The quality of images and the dose of x-ray can′t be separated, which must be quoted together.

The level of the laboratory work is an important symbol for the levels of teaching,research and management in a college.The Logistics university of the chinese people's armed police force is in a institution of transitional restructuring and need to integrate existing research laboratories in order to form a comprehensive experimental center and to build collaborative research platform.On the basis of the current situation and the necessity of a college research laboratory analysis of the Armed Police,we produce the measures and methods and discuss the purpose and significance of the integration.Through these analysis,we both provide the leaders a reference for the development of the construction plan of the hospital research laboratories,but also provide a reference for the management of the research laboratories.At last we can build the scientific research system which the university need and improve the research quality.

Objective To explore the effects of different operations on respiratory function after colon replacement for esophageal cancer.Methods Fourty patients with esophageal cancer underwent esophagectomy from May 2004 to June 2008,were retrospectively analyzed.The site of esophageal anastomosis was at neck after sternum in 20 cages.at neck through esophageal bed in other 20 cages.Blood gas analysis and pulmonary fuction were monitored in these patients before operation and in 14 days after operation.Results VC% were (42.17±10.15)%、(49.52±9.56)%、(55.67±10.73)%、(60.27±10.52)%in patients after sternum and(37.65±9.52)%、(40.72±10.12)%、(47.02±10.65)%、(52.89±10.82)%in patients through esophageal bed in 5th、7th、10th、14th postoperative day with sigllificant statistical difference;FEV_1% were(60.55±16.71)%、(67.12±16.90)%in patients after sternum and(45.23±16.26)%、(50.52±16.72)%in patients through esophageal bed in 10th、14th postoperative day with significant statistical difference;PaO_2 were(17.56±7.32)mm Hg、(19.67±6.08)mm Hg、(17.17±4.85)mm Hg、(15.43±5.02)mm Hg、(11.32±3.79)mm Hg、(9.67±2.87)mm Hg、(6.98±3.26)mm Hg in patients after sternum and(20.17±7.04)mm Hg、(22.83±6.75)mm Hg、(20.67±4.31)mm Hg、(18.32±4.85)mm Hg、(16.02±3.71)mm Hg、(13.44±2.56)mm Hg、(9.01±3.17)mm Hg in patients through esophageal bed in 1st,2nd,3rd,5th,7th,10th,14th postoperative day with significant statistical difference. Conclusions After esophagectomy,esophageal anastomosis through esophageal bed has significant negative effect on respiratory function.

Carcinoma, Renal Cell ; complications ; Humans ; Kidney Neoplasms ; complications ; Lymphoma, Non-Hodgkin ; complications ; Male ; Middle Aged