Objective　To study the effect of hypoxia-ischemia on cysteinyl aspartate-specific proteinases (caspase-3) activity in cerebral tissue of neonatal rat and probe into its significance. Methods　To induce hypoxic-ischemic brain damage (HIBD), the left carotid artery of rats at day 7 was ligated and animals were exposed to 8% oxygen for 2 hours. 0.5,12,24,and 48 hours after HIBD, both ipsilateral and contralateral cerebral tissue were ditected and homogenized. caspase-3 activity was measured by cleavage of the colorimetric substrate DEVD-pNA. Results　Caspase-3 activity in ipsilateral cerebral tissue increased gradually after HIBD and peaked at 24 hours, and then decreased significantly at 48 hours（P<0.001）. There were no significant changes in caspase-3 activity in the contralateral tissue at all time points (P>0.05）. Conclusions　Significant activation of caspase-3 after cerebral hypoxia-ischemia strongly suggests that apoptosis is involved in HIBD. Application of caspase inhibitors or other anti-apoptotic agents may become a new therapeutics of HIBD.

Perihematomal edema is common after hypertensive intracerebral hemorrhage. It is one of the important causes influencing functional recovery. This article reviews the mechanisms of perihematomal brain edema formation after hypertensive intracerebral hemorrhage, particularly, the potential mechanisms of hypertension in the processes of brain edema formation, as well as therapeutic targets of brain edema.

ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

Objective To investigate the reason and prevention of severe hyponatremia in hysteroscope treatment of the submucous myoma.Method Retrospective study was done on 106 cases of submucous myoma,3 cases with severe hyponatremia after hysteroscope treatment.Results The rate of hyponatremia was 2.8％(3/106),patients appeared nausea and vomiting 1 hour after hysteroscope treatment,blood sodium was lower than 110 mmol/L,volume of distention liquid was 8000-10000 ml,the volume of discharge was 6300-7500 ml.Three patients were treated immediately,1 patient was given sodium lactate Ringer injection and 0.9％ sodium chloride by intravenous drip,and furosemide by discontinuous injection 15 hours later,the hyponatremia was recovery; 2 patients were given 3％ sodium chloride and 15％ potassium chloride by intravenous drip,and furosemide by injection,12 hours later,the hyponatremia were recovery.Conclusion The prevention of severe hyponatremia complication after hysteroscope treatment of the submucous myoma not only need operate carefully but also need take active preventive measures.

18 dogs with hemorrhagic shock were divided into 3 groups. Group Ⅰwascontrol, group Ⅱ and Ⅲ were jetted with air or oxygen respectively and continuouslyoutside the glottis by HFJV (frequency 80 beat/min, driving pressure 0.2 MPa, I:E timeratio=1:2) during the uncompensated period. Results: Not much difference was foundbetween group Ⅰand Ⅱ in their blood gas, acid-base balance and plasma malondialdehyde(MDA). However, the P_aO_2, P_aCO_2, CO_2, combining power and plasma MDA in group Ⅲwere markedly higher than those of group Ⅰand group Ⅱ (P

Objective To explore the relationship between nuclear factor ?B (NF ?B) and the occurrence, metastasis, and treatment of colon cancer. Methods The literature on the structure and the property of molecular biology of NF ?B, the relationship between NF ?B and apopotosis, malignant tumor and colon cancer were reviewed.Results NF ?B had action of antiapopotosis. The occurrence of malignant tumor had close relation with the oncogene by NF ?B, the metastasis of malignant tumor was that cell of cancer escaped the killing and supervising of immunity by NF ?B. NF ?B affected the occurrence and metastasis of colon cancer by regulating c myc, Cox2, ICAM 1.Conclusion NF ?B has important action in the occurrence and metastasis of colon cancer. It will become a new target of treatment.

Both ?-cell dysfunction and insulin resistance contribute to the development of type 2 diabetes, and ?-cell function, especially early insulin secretion, is important in maintaining normal glucose tolerance. This review focuses on the ciritical role of ?-cell dysfunction in the pathogenesis of type 2 diabetes.

Objective To investigate the inhibitory effect of appling slow-releasing triptolide on adventitia on the intima hyperplasia of autologous vein graft.Methods In 24 male New Zealand rabbits, external jugular vein to common carotid artery models were established, and then were divided into 3 equal groups at random: blank-control group, receiving no management on adventitia of the vein graft; F-127 control group, receiving local application of 0.5 mL of 20 % F-127 on adventitia of the vein graft; experiment group, receiving local application of 0.5 mL of 20 % F-127 containing triptolide 300?g. Vein graft specimens were harvested at 2 weeks after the operation. Histomorphologic methods were used to detect the degree of intima hyperplasia of the specimens. The expression of bcl-2 and Fas of the specimens was detected by immunohistochemistry. The apoptosis vascular smooth muscle cells (VSMC) were detected by TUNEL.Results Two weeks after vein grafting, compared to blank-control group and F-127 control group, intima hyperplasia of experiment group [intima thickness (29.9?7.6)?m, I/M 0.56?0.08] was markedly inhibited (P

1.CON-CLUSION:Safe,effective and cheap antiinfectives assume a predominant place in our hospital.

ObjectiveTo introduce the effect of trifoil peptides on the protection and treatment of gastrointestinal ulcer(GIU). MethodsWe reviewed literatures about trifoil peptide in its structure, distribution, physiologic effect and mechanism, and its relation with GIU. Results Trifoil peptides formed a layer of mucus glue by combining with glycoprotein ,and improved the defense ability of the gastrointestinal(GI) mucosa .Trifoil peptides repaired the GI mucosa by abjusting the movement of mucosal cells. Trifoil peptides also had the effect of antiproliferation and antiapopotosis. There was an ulcer-associated cell lineage (UACL) around GIU. In GIU, trifoil peptides unusually expressed new cells coming from UACL moved to the surface of ulcer, and repaired the ulcer. UACL can express three trifoil peptides.Used before and after the experiment, trifoil peptides have the protective and therapeutic effect on the experiment gastroenteric ulcer. ConclusionsTrifoil peptides may offer a new method to prevent and treat GIU.