1.The molecular mechanisms of vasovagal syncope.
Ping HUANG ; Hong SHI ; Hong-wei WANG
Chinese Journal of Pediatrics 2006;44(5):387-389
2.Nursing Administration of Peripherally Inserted Central Catheter for Prevention of Infection:A Report of 160 Cases
Xuyi WANG ; Yurong HUANG ; Jian SHI
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To prevent the infection at intravenous injection site with peripherally inserted central catheter(PICC). METHODS The relationship between infection with sites,methods,and times of PICC placement in 160 patients was observed. RESULTS There was no correlated infection in all the patients,but family nursing might lead to infection. CONCLUSIONS Regular management and standard operation can prevent infection.Instructions of family nursing and follow-up are necessary for infection control.
3.The structure and the effector functions of N-glycan of therapeutic monoclonal antibodies
Shengshi HUANG ; Di WANG ; Yikang SHI
Chinese Journal of Biochemical Pharmaceutics 2017;37(4):443-448
More and more therapeutic monoclonal antibodies(mAbs)are widely used for treating various illnesses such as cancer and autoimmune diseases due to the specific and efficient properties of mAbs.All of the currently licensed therapeutic mAbs are of the IgG class.Fc region of IgG has a N-glycan which is structural heterogeneity.The glycan on Fc region has many significant roles on the structure,the effector functions and pharmacokinetics of mAbs.The glycan of Fc region can be changed through genome editing and optimization of cell culture condition.This review presents an overview of the role of the various glycans in the effector functions of therapeutic mAbs and the impact of various parameters on glycosylation pattern of therapeutic mAbs.
4.The diagnosis and treatment progress of invasive fungal sinusitis.
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(11):832-836
The incidence of invasive fungal sinusitis is low, on the rising in recent years. But the misdiagnosis rate of the disease is high. In order to improve the level of early diagnosis and reduce the misdiagnosis, in this pa per, the invasive fungal sinusitis's clinical characteristics, diagnosis, treatment and prognosis are reviewed.
Humans
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Mycoses
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diagnosis
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therapy
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Sinusitis
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diagnosis
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therapy
5.A case of oncocytic papilloma in nasal ethmoid sinus.
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(16):916-917
The incidence of oncocytic papilloma of nasal ethmoid sinus is very low. In order to improve the understanding of the disease for clinicians,we report one case of oncocytic papilloma in nasal ethmoid sinus. The case performs mainly congestion of the left nasal cavity, running pus nose and hyposmia. Nasal endoscopy found new creature in the meatus,and CT indicates soft tissue shadow in the left superior nasal passages,ethmoid sinus involved. Tumor is resected completely. There is no recurrence after five years follow-up.
Aged
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Humans
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Male
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Nasal Cavity
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Nose Neoplasms
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Papilloma
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Paranasal Sinus Neoplasms
6.Evaluation of macular function in open angle and angle-closure glaucomatous eyes by microperimetry
Wang WENQIAN ; Shi, YAN ; Wang, XIN ; Zhang, CHUN ; Huang, PING
Chinese Journal of Experimental Ophthalmology 2014;32(2):149-153
Background The biomeasurement and imageology of retinal nerve fiber layer(RNFL) thickness showed the damage of retinal structure in the early and middle stage of glaucomatous eye,however,the subtle functional damage of glaucoma can not be timely reflected only with automatic static perimeter.Microperimetry is a method of quantitatively assessing mean sensitivity (MS) of macular zone.Objective This study was to evaluate and compare the macular functional change in early and middle stage of primary open angle glaucoma (POAG) and chronic angle-closure glaucoma (CACG) with MP-1 microperimeter.Metbods This trail protocol was approved by Ethic Committee of Peiking University Third Hospital,and written informed consent was obtained from each subject prior to entering the study group.A cross-sectional and case-controlled study was designed.A total of 126 eyes from 126 subjects were enrolled in the trail,including 53 eyes of 53 normal subjects,50 eyes of POAG patients and 23 eyes of CACG patients.A macular 10° program was set with MP-1 microperimetry to record the MS of various subareas.The macula was zoned into central 2°,6°,and 10° visual fields and 4 quadrants in each ring.The MS of different rings and subareas was detected and compared among POAG patients,CACG patients and normal controls.Results The MS values of central 2°,6°,10° and whole macular area were (15.09 ± 3.03),(15.72 ± 3.22),(13.99 ± 3.63) and (14.91±3.07)dB in the POAG group,which were significantly lower than those of (17.29±1.59),(18.05±1.24),(16.76±1.89) and (17.37±1.46)dB in the normal control group (all at P=0.000).The MS values of central 2°,6°,10° and whole macular area was (16.00±2.39),(15.83±2.63),(14.45±3.15) and (15.42±2.54) dB in the CACG group,and the reduced MSs were seen at the 6°,10° rings and whole macular area in the CACG group compared with the normal control group (P =0.004,0.013,0.011).Within the 6° ring,the MS values in the inferotemporal quadrant were declined in the POAG group and CACG group compared with the normal control group (P =0.000,0.022),but the difference was not statistically significant between the POAG group and the CACG group (P =0.311).In addition,the MS value in the inferonasal quadrant was significantly lower than that of the normal control group (P =0.005); while that in the CACG group was not significantly different in comparison with the normal control group (P=0.119).In the POAG group,the MS value of the inferotemporal quadrant was significantly lower than that of the superonasal or superotemopral quadrant (P =0.043,0.016),but no significant differences were found among the 4 quadrants in the CACG group (all at P>0.05).Conclusions The mild damage of retinal function appears in the early and middle stage of POAG and CACG.More serious MS reducing occurs in the inferotemporal and inferonasal quadrants of POAG.
7.Pharmacokinetic Study on Ferulic Acid in Plasma of the Healthy Female Volunteers after Oral Administration of Shenghua Decoction
Yanmei WANG ; Hengjun SHI ; Xi HUANG ; Baoxiu WANG ; Hongquan GAN
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(01):-
Objective To study the pharmacokinetics of ferulic acid (FA) in plasma of the healthy female volunteers after oral administration of Shenghua decoction.Methods Using p-hydroxybenzaldehyde as the internal standard, the plasma concentration of FA was determined by RP-HPLC. Plasma samples were extracted and treated with boiling water and 3P97 programme was used to calculate the pharmacokinetic parameters. Results The main pharmacokinetic parameters of FA were as follows: T1/2?=18.72 min ,T1/2?=79.21 min,T1/2 Ka=11.19 min,AUC=18004.87 ?g?min?L-1,CL=0.17L?min-1?kg-1,Cmax=206.30 ?g?L-1, Tpeak=22.78 min.Conclusion After oral administration of Shenghua decoction, FA could be absorbed and eliminated rapidly and pharmacokinetics of FA conforms to a two-compartment open model.
8.Study on the Healing-promoting Effect of Total Tanshinone on Sore and Ulcer of Model Rats
Hailing WANG ; Hongyong HUANG ; Chuansheng WANG ; Tianyuan SHI
China Pharmacy 2015;(19):2635-2637
OBJECTIVE:To investigate the healing-promoting effect of total tanshinone on sore and ulcer of model rats. METH-ODS:Staphylococcus aureus levitation liquid was made to reproduce model rats with sore and ulcer,sc. 50 rats were randomly di-vided into normal group(equal volume of CMC-Na solution),model group(equal volume of CMC-Na solution),ampicillin group (positive drug 20 mg/kg)and total tanshinone high and low dose groups(200 and 100 mg/kg),ig,once a day for continuous 12 d. The general conditions were observed. The content of lysozyme(LZM)and immunoglobulins G(IgG)in serum were tested af-ter 12 d;the content of LZM and IgG in purulent secretion of model rats were respectively tested after dosing of 4 d,8 d and 12 d. The integral of wound deterioration were evaluated. RESULTS:Compared with normal control group,the index was worse in model group. Compared with model group,the appetite and activity amount of total tanshinone in high dose group (200 and 100 mg/kg)were increased,with no secretion on the wound and a clean escharosis;content of LZM and IgG in serum of rats in in to-tal tanshinone high and low dose group were increased after 12 d;also,the content of LZM and IgG in purulent secretion of rats in total tanshinone high and low dose groups were increased after dosing of 4 d,8 d,and 12 d;the integral of wound deterioration in total tanshinone high dose group were decreased after dosing of 8 d and 12 d,with significant difference (P<0.01 or P<0.05). CONCLUSIONS:Total tanshinone can promote the wound healing on sore and ulcer of model rats by a mechanism that is related to the content of LZM and IgG in serum and purulent secretion.
9.Application progress of uncut Roux-en-Y anastomosis in digestive tract reconstruction after distal gastrectomy of gastric cancer
Yuqin HUANG ; Youquan SHI ; Dong TANG ; Jie WANG ; Daorong WANG
Chinese Journal of Digestive Surgery 2016;15(9):943-946
Digestive tract reconstruction is an important part of gastric carcinoma operation as well as tumor resection and lymph node dissection.Surgeons are seeking the optimal reconstruction method that reduces the occurrence of complications and maintains better quality of postoperative life extremely.Uncut Roux-en-Y anastomosis is a modified procedure in which an artificial jejunal occlusion is devised to avoid Roux stasis syndrome based on Billroth Ⅱ and Braun's anastomosis.Compared to the conventional Roux-en-Y anastomosis,the uncut Roux-en-Y anastomosis retains the advantage of preventing biliary and pancreatic secretions reflux,furthermore,it can decrease the symptoms associated with Roux stasis owing to the abnormal myoelectrical conduction of Roux limb.Because the early studies indicated that there was higher incidence of dehiscence or recanalization of the jejunal occlusion,the uncut Roux-en-Y anastomosis has not been widely applied.Since jejunal occlusion has been optimized recently,the uncut Roux-en-Y reconstruction may be an optional and appropriate method of digestive tract reconstruction after distal gastrectomy.
10.Reversal of multi-drug resistance by vector-based-ShRNA-Mdr1 In Vitro and In Vivo.
Shi, LU ; Qi, HUANG ; Zehua, WANG ; Yinfeng, SONG ; Lijun, WANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2009;29(5):620-4
In order to investigate the effects of vector-based hairpin small interference RNA (shRNA) on the reversal of multi-drug resistance (mdr) of A2780/Taxol cells, a novel vector pEGFP-H1/mdr1 containing mdr1-shRNA targeting at position 2943-2963 of mdr1 was designed and synthesized. Subsequently, A2780/Taxol cells were transfected with pEGFP-H1/mdr1, and the expression of mdr1 mRNA and P-gp was detected by using RT-PCR and Western blot respectively. MTT was used to measure the 50% inhibition concentration (IC(50)) of Taxol to A2780/Taxol cells. The results showed that at the 24th and 48th h after transfection, the expression of mdr1 mRNA was decreased to (52.1+/-1.0)% and (0.01+/-1.7)%, and that of P-gp decreased to (88.3+/-2.1)% and 0%, respectively. At the 48th h after transfection, the relative reversal rate of A2780/Taxol cells to Taxol was 69.54%. In vivo, the nude mice xenografts were injected with pEGFP-H1/mdr1, and then administrated Taxol. The tumor volume in pEGFP-H1/mdr1-transfected group was significantly reduced as compared with that in blank control group or pEGFP-H1-transfected group (807.20+/-103.16 vs 1563.78+/-210.54 or 1480.78+/-241.24 mm(3), both P<0.01). These results suggested that transfection of pEGFP-H1/mdr1 could efficiently down-regulate the expression of mdr1 mRNA and P-gp in A2780/Taxol cells, and effectively restore the sensitivity of A2780/Taxol cells to Taxol both in vitro and in vivo.