1.Effect of human serum albumin on the attachment of human gingival epithelial cells to commercial pure titanium
Yu LIU ; Shenggen SHI ; Yingliang SONG
Journal of Practical Stomatology 2001;0(01):-
objective: To investigate the effect of human serum albumin(HSA)on the attachment of human gingival epithelial cells(HGEs)to commercial pure titanium(cpTi).Methods: HGEs were cultured, the cells of passage 2~5 were seeded on to cpTi samples which were preincubated in keratinocyte serum free medium with 50 mg/ml HSA or without HSA. Cell attachment was studied by immunofluorescence analysis.Results: HGEs were cultured and identified by positive expression of cytokeratin. 4,12 and 24 h after seeding attached cells on HSA preincubated cpTi were 218,730 and 849, those on the control 417,745 and 864, respectively.Conclusion: The early stage of cell attachment of HGEs on to cpTi may be inhibited by HSA.
2.Study on the Application of the Multi-media Means to Pathological Course Teaching
Mumu SHI ; Hong LIU ; Yinli SONG
Chinese Journal of Medical Education Research 2006;0(11):-
In pathological course teaching,multi-media teaching application has promoted pathology teaching tremendously,enriched pathology teaching connotation and widened the students'visual field.It also brings about some negative effect.By discussing the advantages and disadvantages of applying multi-media teaching to pathological mechanism teaching,we can serve the pathological mechanism teaching better.
4.Effect of Ginkgo Biloba extract on the expression of Activin A during the process of hepatic fibrosis
Hao LIU ; Zhaohong SHI ; Wei HU ; Yufang WU ; Song LIU
Chinese Journal of Tissue Engineering Research 2007;0(02):-
AIM:Activin A,a member of transforming growth factor superfamily,is the negative regulator factor in liver regeneration. In this study,the effects of extract of Ginkgo Biloba on hepatic fibrosis and the expression of Activin A in rats with cirrhosis were investigated. METHODS:The experiment was performed at in the Central Laboratory of Wuhan First Hospital from September 2005 to December 2006. ①Thirty-six male SD rats of(160?20) g were randomized into 3 groups:control group,model group and treatment group. ② Except the rats in the control group,others were intraperitoneally injected with 500 mL/L CCl4 for 8 weeks to establish models of hepatic fibrosis. Meanwhile,the extract treatment group was infused with the extract of Ginkgo Biloba(Chinese drugs preparation laboratory of Wuhan First Hospital,detected by Hubeu Wushi Medicine Industry Co.,Ltd. No. 02-391) daily for 8 weeks. ③After administration,all anesthetized rats were sacrificed. Blood samples were collected for the determination of liver function biochemical indexes. Liver tissue samples were used for histopathological examinations. The expression of Activin A was determined by immunohistochemistry and RT-PCR. RESULTS:All 36 rats were involved in the final analysis. ①The liver function in extract treatment group was significantly improved compared with that in model group. ②The grade of fibrosis in extract treatment group were remarkably lower than that in model group under light microscope. ③The positive staining of Activin A in treatment group was significantly reduced compared with model group. ④The expression of Activin A mRNA in extract treatment group was significantly reduced compared with model group. CONCLUSION:Extract of Ginkgo Biloba can effectively decrease the expression of Activin A in rats with hepatic fibrosis caused by CCl4,and lessen the degree of hepatic fibrosis.
5.Effect of Electromyographic Biofeedback Therapy and Kinesitherapy on Radial Nerve Injury
Min LIU ; Song LI ; Chunhui LIU ; Hongling LI ; Hanwen SHI
Chinese Journal of Rehabilitation Theory and Practice 2013;19(2):167-169
Objective To observe the effect of electromyographic biofeedback therapy and kinesitherapy on patients with radial nerve injury.Methods 82 patients with radial nerve injury were divided into control group (n=41) and treatment group (n=41). The control group was treated with medcine and acupuncture, while the treatment group received electromyographic biofeedback therapy and kinesitherapy in addition. The radial nerve function was assessed and analyzed by electromyogram (EMG) and motor conduction velocity (MCV) before and after treatment. Results After 12 weeks of treatment, limb motor function, EMG and MCV were better in the treatment group than in the control group (P<0.05). Conclusion Electromyographic biofeedback therapy and kinesitherapy can effectively improve the limb motor function of patients with radial nerve injury
6.Optimization on Preparation Techniques of Wuji Gastric Floating Sustained-release Tablets by Multicomponent Indicator
Qun WANG ; Wen LIU ; Daye CHEN ; Song YANG ; Xiaowei SHI
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(7):1638-1643
This study was aimed to screen out an optimal pharmaceutical formulation of Wuji Gastric Floating Sus-tained-release Tablets. With multicomponents of release for index, the extra-floating ability of the tablets was stud-ied with the orthogonal test of four excipients, which were HPMC (K100M), PEG 6000, sodium bicarbonate and se-tanol. The results showed that the combined optimal pharmaceutical formulation was 35% HPMC, 5% PEG 6000, 10% sodium bicarbonate, 15% setanol, 10% MCC and 25% main drug. It was concluded that Wuji Gastric Floating Sustained-release Tablets had the characteristics of slow-release and long floating duration which comply with re-quirements of formulation design. This preparation technique is with good maneuverability.
7.Effect of metformin on expression of SIRT3 in skeletal muscle of rats with type 2 diabetes
Yuping SONG ; Chong HAN ; Jingli SHI ; Ying WU ; Zhimin LIU
Chinese Journal of Endocrinology and Metabolism 2013;(5):427-429
[Summary] Type 2 diabetic rat model accompanied by insulin resistance was established by high fat/high glucose diet and streptozotocin.Following metformin treatment for 4 weeks,realtime PCR and Western blot were used to detect the expressions of SIRT3 mRNA and protein in skeletal muscle tissue,respectively.The results showed that insulin sensitivity,superoxide dismutase (SOD),and glutathione (GSH) levels were significantly reduced in diabetic group compared with the control group (P<0.05),while methane dicarboxylic aldehyde (MDA) level was increased (P<0.05),along with the decreased expressions of SIRT3 mRNA and protein in skeletal muscles (P<0.01).After metformin treatment,the insulin sensitivity,SOD,GSH,and SIRT3 mRNA and protein levels were significantly increased (P< 0.05 or P< 0.01),while MDA level was decreased (P < 0.05),suggesting that metformin may ameliorate insulin resistance via upregulating SIRT3 expression in skeletal muscles of diabetic rats.
8.Relationship Between Chlamydia Pneumoniae and Abdominal Aortic Aneurysm
Sen SHI ; Yanzheng HE ; Yong LIU ; Li SONG
Chinese Journal of Bases and Clinics in General Surgery 2003;0(06):-
Objective To investigate the recent studies about the relationship between Chlamydia pneumoniae and abdominal aortic aneurysm. Methods The current literatures about the relationship between Chlamydia pneumoniae and abdominal aortic aneurysm were reviewed. Results Chlamydia pneumoniae is one of the most important factors for the formation of abdominal aortic aneurysm since Chlamydia pneumoniae can cause abdominal aortic aneurysm through the metabolism of matrix metalloproteinases, the apoptosis of smooth muscle cells in the vessels and the chronic infection of the wall of the aneurysm. Conclusion There maybe a distinguishingly close relationship between Chlamydia pneumoniae and abdominal aortic aneurysm, and Chlamydia pneumoiae may take an important role in the development and progress of the abdominal aortic aneurysm.
9.The protective effect of hydrogen sulfide on intestinal ischemia-reperfusion injury in rats
Hao LIU ; Xiaobin BAI ; Song SHI ; Yongxiao CAO
Journal of Xi'an Jiaotong University(Medical Sciences) 2003;0(06):-
Objective To study the protective effect of hydrogen sulfide(H2S) on intestinal ischemia-reperfusion(I/R) injury so as to provide a new clinical treatment on I/R.Methods NaHS was taken as a donor of H2S.Forty Sprague-Dawley rats were divided into 5 groups with 8 rats in each group: sham operation group,I/R model group,tetram ethylpyrazine group,and NaHS 7 ?mol/kg and 14 ?mol/kg groups.The contents of superoxidase dismutase(SOD),malondialdehyde(MDA) and glutathione peroxidase(GSH-Px) in serum and intestinal tissue were measured,respectively.The intestinal mucosal injury and histological alteration were observed.Results The content of MDA within serum and intestinal tissue was significantly reduced and the activities of SOD and GSH-Px were significantly increased.H2S could obviously reduce the injury in intestinal mucosa and glands.Conclusion H2S possesses a protective effect on intestinal I/R injury in rats.
10.Clinical effects of combined fascia sheath suspension and frontal muscle flap suspension in correction of severe congenital ptosis
Junhu SHI ; Shan LIU ; Hongbin ZHANG ; Ping BAI ; Lihua SONG
Chinese Journal of Medical Aesthetics and Cosmetology 2017;23(2):107-110
Objective To compare the therapeutic effect of combined fascia sheath suspension and frontal muscle flap suspension in the treatment of congenital severe congenital ptosis.Methods 46 cases (58 eyes) with congenital severe upper eyelid prolapse who were treated from March 2015 to March 2016 in Eye Hospital of Hebei Province were selected.The patients were divided into two groups:combined fascial sheath suspension group and frontalis muscle flap suspension.Comparisons of postoperative patients with eyelid height,eyelid radian,corneal exposure with eyes closed,eyelid activity,complication of the situation were carried out.And patient satisfaction survey was conducted.Results The effective rate of combined fascial sheath suspension group was 93.10%,the complication rate was 6.9%,and the patient satisfaction rate was 91.22±0.37,significantly better than the frontal muscle flap suspension group of patients (75.86 %,24.14 % and 75.05 ± 0.28,respectively).The time of closure of the eyelid was shortened,the complications were less,and the eyelid had better activity.The difference between the two groups was statistically significant (P<0.05).Conclusions Combined fascia sheath for congenital severe upper eyelid droop is significant,with fewer complications and a higher rate of satisfaction,which is worth clinical promotion.