Objective To investigate the correlation of the prevalence of periodontitis with age and course of disease in patients with type 2 diabetes.Methods A total of 884 patients with diabetes (both sexes,aged 35-79 yr) were involved in present study.Diagnosis of periodontitis was made according to the 1999 WHO standard,and of type 2 diabetes to the 1997 WHO standard.Age,gender,course of diabetes and periodontal state of those patients were recorded.The patients were grouped according to their age (10 yr interval) and course of disease (5 years interval).Based on the grouping,the collected data were input into ACCESS data bank and statistically analyzed with SAS software,and the prevalence of periodontitis were then compared.Results The prevalence of periodontitis in patients with type 2 diabetes increased with the increasing of age and with the prolonging of disease course (P

Humans ; Periapical Diseases ; therapy ; Periodontal Diseases ; therapy ; Tooth Crown ; injuries ; Tooth Diseases ; therapy ; Tooth Root ; injuries

Objective To explore the effects of lactational maternal stress on neurobehavioral development of offsprings.Methods Maternal mice were divided into normal control,maternal deprivation and maternal stress group.A 5-min/day cold water swimming was used as maternal stress for continuously of 14 day since the 7th postnatal day.The increasing bodyweight and neurobehavior of adolescent offspring mice were assessed by forced swimming test,tail suspension test and locomotor activity in the 35th postnatal day.Results Maternal chronic stress in lactational stage significantly increased the vulnerability of offspring to acute stress,which was reflected by significantly increased immobility time of adolescent offspring in forced swimming test (female:(139±6) s,(138±9) s,(96±9) s respectively; male:(139±9) s,(112±9) s,(96±9) s respectively) and in tail suspension test (femal:(127±8) s,(123±8) s,(98±6) s respectively ; male:(141 ±7) s,(105±5) s,(92±6) s respectively).Meanwhile,sexual difference occurred for adolescent offspring to acute stress,and the female offspring were more vulnerable to acute stress.Conclusion Lactational maternal stress may be important factors for development of adolescent depression.Our findings highlight the area of early prevention and intervention for adolescent depression.

ObjectiveTo investigate the impact of health education and psychological intervention on quality of life of patients with asthma.MethodsNinety-six patients with bronchial asthma were assigned to the treatment group and the control group. The participants in the treatment group were treated with routine therapy and health education and psychological intervention.Results The awareness of bronchial asthma,self monitoring of health condition,and simple strategies to treat acute or serious asthma were improved in the treatment group. The overall effective rate of the treatment group was significantly higher than that of the control group (93.75％ vs 62.5％,P ＜0.01 ),and forced expiratory volume in one second (FEV1),peak expiratory flow (PEF) and ACT score were significantly improved (all P ＜ 0.01 ).Moreover,episode of bronchial asthma was significantly reduced after the combined intervention (P ＜0.01 ).ConclusionHealth education and psychological intervention may contribute to effective prevention and treatment of asthma,improved quality of life of patients,and reduced family and social economic burden.

Objective:To evaluate the ability of calcium hydroxide removal using different irrigation methods and the effect on canal sealing.Methods:200 human single root canal premolar teeth were prepared.Divided the teeth randomly into 2 groups(each group 96 teeth,8 as control),one group filled with chemical calcium hydroxide and another filled with finished product calcium hydroxide.7 days after,divided each groups randomly into 6 subsets and remove the calcium hydroxide in the root canal(table1).Then each subset group choose 8 teeth randomly,observed by scanning electron microscope.Other specimens were obturated by lateral condensation of cold gutta-percha with a AH-Plus sealer,stained by Indian ink and then made into transparent teeth model.Results:At the same condition,there was no significant difference between group A and group B;the results of groups associated with ultrasonic were better than groups with injection syringe.The rinse solution EDTA was the best,distilled water was the worse.Dye penetration showed that the length of dye staining was no significant difference among all groups.Conclusion:Ultrasonic and sodium hypochlorite associate EDTA is the best method to remove the calcium hydroxide.But none of the methods and rinse solution used in this experiment can remove all of the calcium hydroxide.The calcium hydroxide remained influence on the root canal sealer is not predominance.

Objective To investigate the effect and mechanism of metformin on endometrial carcinoma subline of progestin-resistance and find whether metformin could regulate progestin-resistance in endometrial carcinoma. Methods Ishikawa endometrial carcinoma cells were cultured for a long period in the presence of the synthetic medroxyprogesterone 17-acetate (MPA) to generate a subline refractory to the growth-suppressive effects of MPA,named Ishikawa/MPA cells. The effect of MPA (1, 5, 10, 20, 40 and 60 μmol/L) or metformin (1, 10, 20, 40, 60, 70 and 80 mmol/L) on proliferation of the Ishikawa/MPA and parental Ishikawa cells was detected by cell counting kit-8 (CCK-8) method. Western blot was used to detect the effect of metformin and(or)MPA on the expression of PR-B in the Ishikawa/MPA and Ishikawa cells. Results The Ishikawa/MPA showed that growth stimulation rather than inhibition in the Ishikawa cells after low MPA concentration treatment. The doubling time of Ishikawa/MPA cell lines were (43±4) hours and that of Ishikawa cell line were (47 ± 3) hours. No changes in doubling time were observed (t=0.349,P=0.572). Low concentration (1 and 5μmol/L) of MPA could promote the growth of Ishikawa/MPA cells (by 3% and 13%). High concentration (10, 20, 40 and 60 μmol/L) of MPA could inhibited the growth of Ishikawa/MPA cells (by 4%, 3%, 9%and 40%) and the growth of Ishikawa cells (by 41%, 55%, 65%and 66%). At the same concentration, the difference of inhibition rates between the two cell lines were statistically significant (P<0.01). Metformin (1, 10, 20, 40, 60, 70 and 80 mmol/L) could inhibite the growth of Ishikawa/MPA (by-10%, 20%, 56%, 89%, 97%, 98%and 99%) greater than those in parental Ishikawa cells (by-6%, 19%, 37%, 54%, 70%, 72%and 83%), and the inhibitory effect was dose-dependent manner. Western blot assay showed that for Ishikawa cells, the protein expression levels of PR-B in metformin group and MPA+metformin group were respectively (53.5±4.0)%and (37.7±5.2)%, which were higher than that in the control group [(23.4 ± 3.0)%], and there were significant the differences (P<0.01). For Ishikawa/MPA cells, the protein expression levels of PR-B in metformin group and MPA+metformin group were respectively (38.6 ± 1.7)%,(36.3 ± 2.5)%,which were higher than those in the control group [(6.4 ± 1.6)%], and there were also significant differences (P<0.01). Conclusion Metformin may regulate the progestin-resistance in endometrial carcinoma by increasing the expression of PR-B.

Objective To explore the change and its clinical significance of neuron-specific enolase (NSE) and S-100? in serum and hippocampus tissue in rats with epilepsy induced by Kainic acid (KA).Methods 180 Wistar rats were randomly classified into control, KA and carbamazepine (CBZ) group, and the later two groups were further divided into 6 sub-groups (1 h, 4 h, 12 h, 24 h, 48 h and 72 h ) according to epileptic attack at different time point. The NSE and S-100? concentrations in serum and homogenate of hippocampus were determined by radioimmunity assay (RIA) and enzyme linked immunosorbent assay (ELISA), respectively.Results The concentrations of NSE and S-100? were dynamic change during 72 h after epileptic attack in serum and hippocampus homogenate, and the changes were synchronous. The concentrations reached peak at 12 h. Both NSE and S-100? concentrations in KA and CBZ group were obviously higher than those in control group ( P

Objective To explore the clinical results of minimally invasive therapy for femoral intertrochanteric fractures with proximal femoral nail anti-rotation(PFNA) in 25 elderly patients.Methods A total of 25 patients with intertrochanteric fractures were treated by PFNA in our hospital from February 2007 to June 2008.The patients received close reduction and minimal invasive operation.Based upon the Tronzo-Evans classification,type Ⅰ fractures were diagnosed in 2 cases,type Ⅱ fractures in 2 cases,type Ⅲ in 16,and type Ⅳ in 5.Results The mean operation time in this series was 50.3 min(30-70 min),an the mean intraoperative blood loss was 100.2 ml(70-200 ml).All the 25 patients completed a follow-up of 3 to 12 months(average time 7.1 months),during which all the fractures healed in 10 to 14 weeks with a mean of 11 weeks,without occurrence of varus deformity,hardware loosening or hardware cutting.According to the Harris Hip Score,20 cases achieved excellent results,4 were good,and 1 was fair. Conclusions It is a reliable and ideal method to treat intertrochanteric fractures with PFNA in elderly patients,for the procedure is convenient and minimally invasive,resulting in firm fixation,less blood loss,short operation time,and high healing rate.

Objective:To evaluate the equity of the secondary performance allocation in target hospital.Methods:Through the comparison on the Gini coefficient calculation,the calculation method of Gini coefficient developed by Jianhua Zhang was applied to estimate the secondary allocation result of performance salary in the target hospital from 2014 to 2015.Results:From 2014 to 2015,the Gini coefficient remained at 0.22 or so,the overall allocation was average.Conclusion:Estimating the Gini coefficient was beneficial for the hospital managers control the structure and level of the second performance distribution and modulate the program of the sencond performance distribution in time,so that the distribution of performance salary would be accordant to the designed program and acts as the direction of motivation.

Humans ; Tooth Crown ; pathology ; Tooth Diseases ; therapy ; Tooth Root ; pathology