Acute Disease ; Adult ; Age Factors ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Occupational Exposure ; prevention & control ; Pesticides ; poisoning ; Poisoning ; epidemiology ; mortality ; prevention & control ; Rural Population ; Sex Factors ; Survival Rate

Objective To evaluate the safety、feasibility and the long-term effectiveness of polyurethane stent placement for treatment of nasolacrimal duct obstruction. Methods Fifty-three cases (64 eyes) with complete nasolacrimal duct obstruction received the lacrimal stent placement. Dacryocystography was performed before and after the procedure. The average follow-up period was 48.3 months (range, 13-78months), the long-term patency and the effectiveness after stents removal were analyzed. Results Stent placement was technically successful in 62eyes, and the success rate was 96.9% with no severe complications. On long-term follow up, the stent patency rate was 76.8% at 1 year. However, it decreased to 33.3% at 3 years and 28.6% at 5 years. Nine stents were removed when the symptoms recurred, including granulation tissue in 4 stents and mucous material in 5. After removal, 2 nasolacrimal ducts were patent, and 2 kept patent after stents replacement, but all reoccluded within 6 months. Conclusions Interventional placement of polyurethane stent in the obstructed nasolacrimal duct is a simple and safe outpatient procedure, but the long-term patency is not encouraging, and the right stent removal time has not yet been approved.

Prostate cancer is one of the common malignant tumors of the urinary system and mostly found in elderly men. Like most tumors, prostate cancer involves a variety of molecules in its occurrence and progression. More studies on the development of prostate cancer focus on the tumor markers, DNA damage repair related genes, and tumor invasion and metastasis related factors. This article presents an overview on the research progress in these three aspects.
Biomarkers, Tumor ; Biomedical Research ; DNA Repair ; Disease Progression ; Humans ; Male ; Neoplasm Invasiveness ; Prostatic Neoplasms ; genetics ; pathology

ObjectiveTo investigate the effect of N-ras oncogene expression in acute myelogenous leukemia(AML).Methods N-ras oncongene in 10 cases of AML were determined using reverse transcriptase-polymerase chain reaction(RT-PCR).Results 3/10 cases were the positive expression,the positive rates were 30%;2/3 patients were treated to complete remmision(CR).Their N-ras oncogene change negative,1/3 patients was no remmision(NR),his N-ras oncogene change negative,1/3 patient was no remmision(NR),his N-ras oncogene keeps positive and dead after 4 months.Conclusions Determination of the N-ras oncogene expression in AML has worthy for diagnose and prognosis.

Objective:To observe the clinical effect and adverse reaction of capecitabine as first-line monotherapy in elderly patients with stage Ⅱa breast cancer.Methods:From June 2002 to June 2005,71 elderly patients with stage Ⅱa breast cancer received chemotherapy(different scheme:capecitabine group and CEF group) after operation.The efficacies and adverse reactions were evaluated and compared between the two groups.Results:The 3-year and 5-year survival rates of patients in capecitabine group were 97.06% and 94.12%,respectively; the relapse rate was 5.88%; all were comparable to those of CEF group. One of the advantages of capecitabine was its oral administration. The adverse effect of capecitabine was mainly hand-foot syndrome,with an incidence of 82.35%,but was tolerable. The gastrointestinal reaction and bone marrow repression in capecitabine group were significantly lower than those in the CEF group(P

Objective:To investigate the expression and correlation of cyclooxygenase-2(COX-2) and c-erbB-2 in human breast cancer.Methods: The expressions of COX-2 and c-erbB-2 in 48 breast cancer patients with breast cancer were detected with immunohistochemistry examination and was analyzed.The relationship between their expression and the clinicopathologic characters of breast cancer was also analyzed.Results: The positive rate of COX-2 was 60.4%(29/48) in these patients;the expression was higher in patients with tumor size larger than 2 cm,lymph node metastasis,and negative expression of ER or PR,but was not related with the ages,the pathologic types and the TNM stages.Expression of c-erbB-2 was identified in(39.6%)(19/48) of these patients;the expression was significantly higher in patients with lymph node metastasis or negative expression of ER and PR,but was not related with the ages,the sizes and the pathologic types.COX-2 was positive in 84.2%(16/19) of patients positive of c-erbB-2 and in 44.8%(13/29) of patients negative of c-erbB-2,with significant difference found between the 2 figures(P=0.006).Conclusion: The high expression of COX-2 in breast cancer is related to the expression of c-erbB-2,suggesting that they may regulate each other and jointly contribute to the tumorigenesis and progression of breast cancer.

Objective To investigate the effect of high pressure distention on the expression of stenosis-related genes of saphenous vein graft(SVG) during the coronary artery bypass grafting(CABG).Methods The biopsy specimens of saphenous vein collected from 10 patients who have undergone CABG,were divided into expansion group and no-expansion group.Real-time PCR and immunohistochemical staining were performed for examination of mRNA and protein expression of VE-cad,Egr-1,VCAN respectively.Student's t and Chi-square test were used to do statistic analysis.Results The results of RT-PCR showed that the mRNA transcription of Egr-1,VCAN in the expansion group were statistically significantly higher than those in no-expansion group(P<0.05).The mRNA transcription VE-cad in expansion group was statistically significantly lower than that in the no-expansion group(P<0.05).The immunohistochemical staining results showed that the expression of Egr-1 and VCAN in expansion group were significantly stronger than those in no-expansion group,while the expression of VE-cad was significantly lower than no-expansion group.Conclusion The intraoperative expansion of SVG can increase the expression of stenosis-related genes Egr-1 and Versican,and decrease the expression of stenosis-related gene VE-cad,which may be related with the SVG stenosis after CABG.

Objective To evaluate the safety of a self-made vena cava stent-filter(VCSF)for prevention of pulmonary embolism.Methods Fusiform unmbrella-like vena cava filter was made of Nitinol wires and stainless steel metal pole,and then ten mongrel dogs were implanted with these self-made filters and divided into 5 groups according to the different periods(2,3,4,5 and 6 w) of filter placement,with 2 dogs in each group.After the VCSFs were placed in inferior vena cava via the right femoral vein approach,the dogs in each group were bred for 2-6 weeks,respectively.The blood flow of inferior vena cava and the position of the filters were inspected by inferior vena-cavography according to the indwelling periods.Finally the metal pole was retrieved via the femoral vein,leaving the VCSF as permanent venous stent.The feasibility of retrieval and the free state of filter net with the adhering vascular wall were evaluated.Laparotomies were performed to remove the inferior vena cava from the animals for gross and electron microscopic examinations of the inferior vena cava intimal changes of the involved segment.Results All 10 VCSFs were placed at the right positions of the dogs successfully.Angiography showed patent inferior vena cava without filter thrombosis at 2-6 weeks.There were no tilting and migration of the filter and all the metal poles were successfully retrieved.The superior and inferior extremities of filter nets could be set free with all the filters turning into venous stents.Postmortem displayed retroperitoneal hemorrhage and caval thrombosis.The barbs of the filters penetrated over the caval adventitial coat.Under electron microscope,a thin layer of neointima already covered the braiding net of VCSFs at 2 weeks after the deployment.The tunica intima became slightly thick at 3-4 weeks and with moderate proliferation at 5-6 weeks.Conclusions The self-made vena cava stent-filter possesses rather long indwelling period according to the necessity of treatment with simultaneously keeping patent caval flow,stability of the position and easy for displacement and retrieval.

Ten compounds were isolated from the 70% ethanol extract of linseed meal (Linum usitatissimum L) through a combination of various chromatographic techniques, including silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC. On the basis of spectroscopic data analysis, they were elucidated as 1-methylethyl-2-O-beta-D-glucopyranosyl-(1" --> 6')-beta-D-glucopyanoside (1), linustatin (2), neolinustatin (3), lotaustralin (4), linamarin (5), deoxyguanosine (6), deoxyadenosine (7), (+)-pinoresinol-4'-O-beta-D-glucopyranoside (8), 4-O-beta-D-glucopyranosylvanillyl alcohol (9) and tachioside (10), separately. Among them, compound 1 is a new compound, and compounds 6, 8 and 10 were isolated from the linseed meal for the first time.

Objective To understand the progress in schistosomiasis resoareh over the last l0 years through a biblimetrie approaeh.Methods The literature was searched in Web of Science citation database (1998-2008)by using the following terms: schistosomiasis* or schistosoma or schistosome. The searching results were analyzed by the Derwent Analytics software concerning the article numbers and the total citations by countries,institutes and authors, and journal distribution, in an effort to discover schistosomiasis research progress in and outside China. Results The annual number of schistosomiasis articles published in Web of Science had little change from 1998 to 2008.The US phyed a leading role in the schistosomiasis research in the world.China had made a great progress in schistosomiasis research in the past years.but Was still lagging behind developed countries.The vaccine and drug development,the immune mechanisms resulting in morbidity were the focuses of schistosomiasis research in the world.Meanwhile.the transcriptome and proteome of Schistosoma japonicum,schistosomiasis vaccines and drugs,spatial epidemiology Were the focuses of research in China.Conclusion The research of schistosomiasis in China has been progressing rapidly,but is still lagging behind its international peers.