1.Comparison of TNM and Lugano staging systems in predicting 5-year survival rate of primary gastrointestinal lymphoma patients
Shujian CHANG ; Xin SHI ; Zhenyu XU ; Quan LIU
Chinese Journal of Clinical Oncology 2015;(7):392-396
Objective:To assess the survival-predictive value of TNM and Lugano staging systems in patients with primary gastro-intestinal lymphoma (PGL). Methods:A total of 73 patients with PGL were recruited from February 2001 to August 2013. All patients were diagnosed according to the TNM and Lugano staging systems. Five-year survival rate was used as the major clinical outcome. Sur-vival curves were plotted using the Kaplan–Meier method and analyzed with the log-rank test. The prognostic value of different vari-ables for clinical outcomes was assessed using the Cox multiple regression model. Results:The median follow-up time of surviving pa-tients was 42.4 months (range:1.3-158.6 months). The estimated 5-year overall survival rate was 77.82%. When diagnosed with the TNM system, the 5-year survival rates in stagesⅠ,Ⅱ,Ⅲ, andⅣwere 100%, 90.0%, 67.4%, and 22.2%, respectively (χ2=17.7956, P=0.0005). When staged by the Lugano system, the 5-year survival rates in stagesⅠ,Ⅱ,ⅡE , andⅣwere 100%, 100%, 70.7%, and 46.2%, respectively (χ2=15.6776, P=0.0013). Cox analysis showed that the invasion depth (T) (P=0.0181) and metastasis (M) (P=0.0031) were covariates that were prognostically significant for the overall survival. Conclusion:The TNM staging system is more ac-curate than the Lugano system in predicting the 5-year survival rate of patients with PGL.
2.Correlative study between MRI signal intensity of the lumbar intervertebral disc and collagen content in the nucleus pulposus
Wen-Chang YU ; Jian-Yong YANG ; Wen-Quan ZHUANG ; Yun-Bin CHEN ; Shi-Fang ZHENG ;
Chinese Journal of Radiology 1999;0(10):-
Objective To study correlation between MRI signal intensity of the lumbar intervertebral disc and collagen content in the nucleus pulposus.Methods Thirty-one cases with lumbar intervertebral disc herniation(male 21,female 10)received percutaneous lumbar disketetomy.Thirty-one specimens of nucleous pulposus were obtained from percutaneous lumbar disketctomy procedure and collagen content in them was measured with reformed hydroxyproline measurement method.The signal intensity(SI) of lumbar intervertebral disc and cerebrospinal fluid was measured in T_2 WI sagittal image and then ratio of disc SI to cerebrospinal fluid SI was calculated.The Pearson analysis was used to analyze the correlation between the collagen content and SI ratio and disc SI on T_2 WI.Results Collagen content and SI ratio were (231.0?63.5)mg/g and 0.19?0.07,respectively.There was negative correlation between them (r=-0.61,P
3.Problems and strategies in the research of patient-derived gastric cancer xenograft models
quan Bin HU ; Bo CAO ; hong Chang SHI
Acta Laboratorium Animalis Scientia Sinica 2017;25(6):643-647
In this paper, we summarize the major problems existing in establishing gastric cancer patient-derived xenograft ( PDX) models and its solutions, and introduce its advantages on screening targeted drugs. As many previous re?search emphasized on individual characteristics too much, we argue that we should pay more attention to the generality of what we are studying in order to analyze the genotype of PDX models before taking a targeted therapy.
4.Study on apoptosis in myelodysplastic syndromes by DNA in situ end labelling combined with APAAP.
Xiao LI ; Quan PU ; Yizhi LIU ; Jun SHI ; Ying TAO ; Chunkang CHANG ; Qinyan JIANG ; Wei HUANG
Chinese Journal of Hematology 2002;23(1):27-29
OBJECTIVETo investigate the total in situ apoptotic cell number and the apoptotic situation in erythroid cell and megakaryocytes in patients with myelodysplastic syndromes (MDS).
METHODSApoptosis cell number and the apoptotic situation of erythroid cell and megakaryocytes were analysed on cold embedded bone marrow sections from 25 MDS patients by DNA in situ end labelling (ISEL)/alkaline phosphatase anti-alkaline phosphatase (APAAP) double stained techniques. Fourteen cases of iron deficiency anemia (IDA) were taken as control.
RESULTSMean apoptotic cell numbers in MDS and control group were (39.44 +/- 29.34)/mm(2) and (13.43 +/- 8.39)/mm(2) respectively (P < 0.01). RA/RAS subtypes had a higher apoptosis ratio (47.56 +/- 32.86/mm(2)) than that in RAEB/RAEB-t subtypes (21.87 +/- 13.65/mm(2)) (P < 0.05). Double staining showed similar apoptosis percentage in erythroid cell and megakaryocytes in MDS patients comparing with that of controls (P > 0.05). Some apoptotic cells showing erythroid or megakaryocytic morphologic characteristics expressed no cluster differentiation antigen.
CONCLUSIONOverapoptosis existed in MDS, RA/RAS group had a higher apoptosis ratio than RAEB/RAEB-t group. No obvious increased apoptosis in erythroid cell and megakaryocytes was observed in MDS perhaps due to the loss of surface antigens in later stages of apoptotic cells.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Alkaline Phosphatase ; immunology ; Apoptosis ; Bone Marrow Cells ; cytology ; metabolism ; Child ; DNA ; genetics ; Female ; Humans ; Immunoenzyme Techniques ; In Situ Nick-End Labeling ; Male ; Middle Aged ; Myelodysplastic Syndromes ; genetics ; pathology
5.Anti-tumor effects of triptolide on osteosarcoma cells in vitro and in vivo: an experimental research.
Qian-Wei SHI ; Shu-Guang LI ; Jun LI ; Chang-quan LING
Chinese Journal of Integrated Traditional and Western Medicine 2013;33(5):659-663
OBJECTIVETo observe in vitro and in vivo effects of triptolide on growth inhibition and apoptosis of osteosarcoma cells, and to further explore its correlated molecular mechanisms.
METHODSThe growth inhibition effects of triptolide on osteosarcoma cells were detected using MTT assay. The apoptosis was detected using flow cytometry.The protein expressions of associated signals were detected using Western blot. The in vivo anti-osteosarcoma effects of triptolide were verified in osteosarcoma nude mice. The in vivo associated protein expressions were detected using immunohistochemistry (IHC).
RESULTSTriptolide could significantly inhibit the proliferation of various osteosarcoma cells. Besides, it could induce their apoptosis. Triptolide triggered the mitochondrial dependent apoptotic pathway, significantly inhibited the in vivo growth of osteosarcoma cells, and caused in vivo apoptosis.
CONCLUSIONSTriptolide induced apoptosis of osteosarcoma cells partially through activating mitochondria associated apoptosis signal pathway. Triptolide also induced apoptosis of osteosarcoma cells and inhibited their in vivo growth in nude mice.
Animals ; Apoptosis ; drug effects ; Cell Line, Tumor ; Diterpenes ; pharmacology ; Epoxy Compounds ; pharmacology ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Osteosarcoma ; pathology ; Phenanthrenes ; pharmacology ; Xenograft Model Antitumor Assays
6.Study about proliferation and apoptosis of megakaryocytes in patients with myelodysplastic syndromes.
Xiao LI ; Quan PU ; Yi-Zhi LIU ; Jun SHI ; Ying TAO ; Chun-Kang CHANG ; Qin-Yan JIANG ; Wei HUANG
Journal of Experimental Hematology 2002;10(1):40-43
In order to observe the proliferative and apoptotic situation of megakaryocytes in patients with myelodysplastic syndromes (MDS). CD41 immunoenzyme labeling (alkaline phosphatase anti-alkaline phosphatase APAAP)/DNA in situ end labelling (ISEL) double stained techniques was used onto plastic cold embedded bone marrow sections in 29 MDS patients to analyse the proliferative and apoptostic characterization of megakaryocytic line with 14 cases of iron deficient diseases (IDA) as control. The results showed that the mean CD41 positive cell number in MDS group was (26.23 +/- 8.18) /mm(2) with a count of (15.64 +/- 7.11) /mm(2) in control group (p < 0.05). The small-micro megakaryocytes in MDS is much higher than that in IDA group (P<0.01). There was a positive co-relation between total megakaryocytes and small-micro megakaryocytes count in MDS (r = 0.702, p<0.01). Some megakaryocytes distributed abnormally around trabecula and formed small or large clusters. Apoptotic megakaryocytes in MDS occupied 4.40% and 9.32% of all CD14 positive cells and all apoptotic cells respectively (p > 0.5 comparing with control). Apoptosis in megakargocytic line occurred only in small-micro megakaryocytes and showed positive co-relation to the number of micro-megakaryocytes. Some apoptotic cell with morphologic characters of megakaryocytes expressed no CD41. It is concluded that overproliferation of megakaryocytes exists in MDS. Apoptosis occurring in micro-megakaryocytes may be a kind of physiological response to abnormal megakaryopoicsis in MDS. No obvious increased apoptosis of megakaryocytes in MDS was found perhaps due to lack of surface antigens CD41 in some later stages of apoptotic cell.
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Apoptosis
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Cell Division
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Child
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Female
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Humans
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Male
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Megakaryocytes
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pathology
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Middle Aged
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Myelodysplastic Syndromes
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pathology
7.Inhibitory effects of Notch receptor blocker MWI67 on the proliferation of U87 glioma cells in vitro
Jian-Quan YANG ; Wei-Cheng YAO ; Shi-Fang LI ; Chang-Qing DING
Chinese Journal of Neuromedicine 2008;7(12):1231-1233,1241
Objective To observe the effect of Notch receptor blocker MW167 on the proliferation and apoptosis of U87 glioma cell line in vitro. Methods The inhibitory effect of MW167 on the proliferation of U87 cells was investigated by MTT assay, and flow cytometry with Annexin-FITC and PI staining was performed to detect the apoptotic rate of U87 cells after MW167 treatment. Results MTT assay demonstrated an obvious concentration-dependent inhibitory effect of MW167 on the proliferation of U87 cells. Flow cytometry showed that MW167 also concentration-dependently induced U87 cell apoptosis. Conclusion MW167 significantly inhibits the proliferation and induces apoptosis of U87 glioma cells, suggesting the potential therapeutic value of MW167 in the treatment of human glioma. [Key wnrds] Notch signaling;MWI67;Proliferation;Apoptosis;Glioma
8.Clinical value of (18)F-FDG positron emission tomography-computed tomography in local liver neoplasm ablation.
Wang-jun LIAO ; Hu-bing WU ; Jin-zhang CHEN ; Min SHI ; Chang-xuan YOU ; Quan-shi WANG
Journal of Southern Medical University 2009;29(8):1641-1642
OBJECTIVETo assess the value of (18)F-fluoro-2-deoxy-D-glucose positron emission tomography-computed tomography ((18)F-FDG PET-CT) in ultrasound-guided local ablation of malignant liver tumors.
METHODSThirteen patients with 35 local residual tumor foci following previous tumor ablation underwent (18)F-FDG PET-CT and ultrasound-guided local ablation with intratumoral alcohol injection.
RESULTSAfter the second local ablation guided by (18)F-FDG PET-CT and ultrasound, radioactive defects were detected in the corresponding location in 31 of the 35 residual foci, and after the third local ablation, the other 4 foci also showed radioactive defects.
CONCLUSION(18)F-FDG PET-CT can sensitively and accurately identify tissue necrosis and residual tumors, and serves as an excellent approach for ultrasound-guided local ablation of local residual tumors.
Ablation Techniques ; adverse effects ; Adult ; Aged ; Female ; Fluorodeoxyglucose F18 ; Humans ; Liver Neoplasms ; diagnosis ; diagnostic imaging ; surgery ; Male ; Middle Aged ; Positron-Emission Tomography ; Postoperative Complications ; Retrospective Studies ; Tomography, X-Ray Computed ; Treatment Outcome
9.Pharmacokinetics and distribution of 5-Fu magnetic albumin deuto-microsphere in normal and tumor-bearing mice.
Rong XU ; Shao-Jun SHI ; Shun-Chang ZHOU ; Jian-Wei ZHENG ; Hui CHEN ; Sheng-Quan ZOU ; Fan-Dian ZENG
Acta Pharmaceutica Sinica 2007;42(1):66-70
To observe the pharmacokinetic and tissue-distribution characters of 5-flourouracil magnetic albumin deuto-microsphere (5-Fu-MAD) in normal and tumor-bearing mice, HPLC method for the determination of 5-Fu in plasma and tissues was established and applied to determine 5-Fu in mouse plasma and tissue samples. A Flame atomic absorption spectrometer was used to detect the iron concentration in mouse tissue. Plasma concentration-time curves of free 5-Fu, 5-Fu-MAD and 5-Fu-MAD plus the magnetic frame (MF) conformed to two compartment model of first order absorption and they had C(max) of 34.9, 7.95 and 5.97 mg x L(-1); T1/2 (Ke) of 22.26, 76.0 and 124.6 min, V(d) of 3.28, 30.7 and 66.1 L x kg; AUC(0-t), of 233.9, 78.3 and 50.2 mg x min x L(-1); AUC(0-infinity) of 237.2, 89.3 and 68.1 mg x min x L(-1), respectively. The distribution of 5-Fu and iron was the highest in the plenty blood perfusion organs like the liver, tumor, spleen and lung, while lower in the kidney and heart and lowest in brain and muscle. The tissue distribution of muscle and tumor increased significantly when a magnetic frame was inserted there. The pharmacokinetics and tissue distribution of 5-Fu-MAD exhibited sustained-release and target characteristics.
Albumins
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chemistry
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Animals
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Antimetabolites, Antineoplastic
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administration & dosage
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pharmacokinetics
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Area Under Curve
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Cell Line, Tumor
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Delayed-Action Preparations
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Female
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Fluorouracil
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administration & dosage
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pharmacokinetics
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Liver
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metabolism
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pathology
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Liver Neoplasms, Experimental
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metabolism
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pathology
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prevention & control
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Magnetics
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Male
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Mice
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Microspheres
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Random Allocation
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Tissue Distribution
10.Disease gene screening of known loci in a Chinese family with autosomal dominant retinitis pigmentosa.
Wei LIU ; Fang LU ; Li-feng QIA ; Zhi-quan SHA ; Xia-oqi LIU ; Shi MA ; Xin TANG ; Jin-xia CHANG ; Zheng-lin YANG ; Bin YE
Chinese Journal of Medical Genetics 2009;26(1):70-73
OBJECTIVETo map the disease-causing gene in a Chinese family with autosomal dominant retinitis pigmentosa.
METHODSTwenty-seven micro-satellite markers were randomly selected from the region around the known loci of causative genes, and haplotypes were determined by ABI3100 genetic analyzer. Two-point linkage analysis was performed using MLINK.
RESULTSThe Lod score of each marker vs adRP was below 1.
CONCLUSIONThe phenotype of this family may not be caused by mutation of the known disease-causing genes.
Asian Continental Ancestry Group ; genetics ; China ; Female ; Genes, Dominant ; Genetic Linkage ; Genetic Testing ; Humans ; Male ; Microsatellite Repeats ; genetics ; Mutation ; Pedigree ; Phenotype ; Retinitis Pigmentosa ; diagnosis ; genetics ; pathology