1.History and development of spinology
Journal of Integrative Medicine 2003;1(4):304-8, 320
Spinal disease is the injury caused by congenital malformation, degeneration, inflammation and trauma. These injuries lead to structural lesion of spinal itself, intervertebral discs, spinal cord, nerve root and the attachment, and can cause spinal and associated diseases. Spinal diseases include some frequently encountered diseases, as well as some stubborn and serious diseases. These diseases may cause pain of head and neck, shoulder and arm, waist and leg when they are mild, and partial or thorough paralysis when they are serious. In this article, the history, development, foundations and research progress of non-surgical operation of spinal diseases and the developing tendency of modern spinology are introduced.
3.Ktrans of dynamic contrast enhanced MRI in evaluation of anti-angiogenic effects on nude mice with orthotopic transplantation tumor model of gastric cancer
Xuelian SHI ; Gaofeng SHI ; Xiaoling WANG ; Qi WANG ; Hui LIU
Chinese Journal of Medical Imaging Technology 2017;33(6):843-847
Objective To assess the feasibility of K value of dynamic contrast enhanced MRI (DCE-MRI) in evaluation of anti-angiogenic effects on nude mice with orthotopic transplantation tumor model of gastric cancer.Methods Nude mice with orthotopic transplantation tumor model of gastric cancer were randomly assigned to two groups:Treatment group (n =15),mice were given apatinib intragastrically for 18 days (100 mg/kg),and control group (n=15),mice were given ddH2O2 in the same manner.After 18 days,DCE-MRI was performed and K value was measured.Then the tumors were dissected from the adjacent tissues in order to detect the microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression levels.MVD and VEGF expression level were compared between treatment group and the control group.Results MVD (x2 =4.89,P<0.05) and VEGF expression level (x2 =8.69,P<0.01) of treatment group were much lower than those of control groups.The Kt value of treatment group was significantly lower than that of control groups ([0.63±0.05]/min vs [1.66±0.23]/min,t=17.05,P<0.01).Conclusion The value of k in DCE-MRI can be utilized to assess the effects of apatinib on nude mice with orthotopic transplantation model of gastric cancer.
5.Histomorphology and ultrastructure in the rat model with cervical vertebral unbalance of dynamic and static force
Chenguang LI ; Yongjun WANG ; Qi SHI
Orthopedic Journal of China 2006;0(05):-
[Objective]To observe the histomorphology and ultrastructure of the intervertebral disc(I D) in the rat model with cervical vertebral unbalance of dynamic and static force.[Method]Sixty SD rates were randomly divided into 4 groups: 3 months,5 months,7 months and the control group.The cervical I D degeneration model was made by destroying the neck muscle of rats and the tissues wer collected every month.The number,area and thickness of the cartilage end-plate were measured by the means of Miyamoto's Classes.The ultrastructure of the apoptosis cells of the intervertebral disc was observed under the electron microscope.[Result]Compared with the control group,the 3 months group showed degeneratin changes,with disordered structure of the annular fibrosis and thickness increase of calcification layer and decrease of blood vessel unmber in the cartilage layer.Complete fibrosis was found in nucleus pulposus in 5 months model group,with the fibro lamellar structure disappeared and few blood vessel buds.The features of 7 months model group was similar to 5 months and osteophyma had been formed near the bordr of part intervertebal.Under the electron microscope,the number of surface projection and organelles was decreased.Fatty drop and apoptotic body could be seen in disc cells of 3 month model group.Few cells,broken collagen fibers in ECM and more cavitation cells for necrosis in 5 months and 7months model groups could be seen.[Conclusion]The cervical I D of model groups has shown typical morphological changes of degeneration and this trend was more serious with the time passing.In the early and middle stage of degeneration,the apoptosis cells can be seen,but in the terminal stage the cellular necrosis was more common.
6.The brief discussion on the anti-epileptic drugs withdrawal in patients with epilepsy
Xiaoshan WANG ; Jintao SUN ; Qi SHI
Chinese Journal of Neurology 2021;54(1):75-78
Epilepsy is the common neurological disease, and antiepileptic drugs are the main means for the treatment of epilepsy. However, there is no conclusion on the timing of drug withdrawal in patients with epilepsy. In recent years, some large-scale prospective studies have provided some guidance on anti-epileptic drugs withdrawal. This article focused on the factors affecting the prognosis and timing of drug withdrawal, hoping to further the understanding of anti-epileptic drugs withdrawal and instruct clinical practise.
7.Reversal of multi-drug resistance by vector-based-ShRNA-Mdr1 In Vitro and In Vivo.
Shi, LU ; Qi, HUANG ; Zehua, WANG ; Yinfeng, SONG ; Lijun, WANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2009;29(5):620-4
In order to investigate the effects of vector-based hairpin small interference RNA (shRNA) on the reversal of multi-drug resistance (mdr) of A2780/Taxol cells, a novel vector pEGFP-H1/mdr1 containing mdr1-shRNA targeting at position 2943-2963 of mdr1 was designed and synthesized. Subsequently, A2780/Taxol cells were transfected with pEGFP-H1/mdr1, and the expression of mdr1 mRNA and P-gp was detected by using RT-PCR and Western blot respectively. MTT was used to measure the 50% inhibition concentration (IC(50)) of Taxol to A2780/Taxol cells. The results showed that at the 24th and 48th h after transfection, the expression of mdr1 mRNA was decreased to (52.1+/-1.0)% and (0.01+/-1.7)%, and that of P-gp decreased to (88.3+/-2.1)% and 0%, respectively. At the 48th h after transfection, the relative reversal rate of A2780/Taxol cells to Taxol was 69.54%. In vivo, the nude mice xenografts were injected with pEGFP-H1/mdr1, and then administrated Taxol. The tumor volume in pEGFP-H1/mdr1-transfected group was significantly reduced as compared with that in blank control group or pEGFP-H1-transfected group (807.20+/-103.16 vs 1563.78+/-210.54 or 1480.78+/-241.24 mm(3), both P<0.01). These results suggested that transfection of pEGFP-H1/mdr1 could efficiently down-regulate the expression of mdr1 mRNA and P-gp in A2780/Taxol cells, and effectively restore the sensitivity of A2780/Taxol cells to Taxol both in vitro and in vivo.
8.Clinical application of wire guided localization of breast biopsy under X-ray guidance
Qi CHEN ; Lihui SHI ; Zhi LI ; Jing WANG ; Teng ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2017;24(19):2910-2913
Objective To study the diagnostic and treatment value of wire guided localization of breast biopsy for non-palpable breast lesions.Methods The clinical data of 314 cases of non-palpable breast lesion resection operation in our hospital were retrospectively analyzed.The accuracy rate of resection and postoperative pathological examination results were analyzed.Results All lesions were positioned accurately and completely resected,48 cases in 314 cases were breast cancer(15.3 %),in which 42 cases were early breast cancer which were confirmed by pathology (87.5%).Conclusion Wire guided localization of breast biopsy has high accuracy,low cost,which is useful for the diagnosis of early breast cancer,there is a certain value in clinical application.
9.Effect of telomerase-antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide
Zhihua DENG ; Ziyan HAN ; Qi WANG ; Shuisheng SHI
Chinese Journal of Cancer Biotherapy 2006;0(06):-
Objective: To observe the effect of telomerase-antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide, in an effort to look for a new anti-hepatic cancer agent with high efficiency, low cytotoxicity. Methods: We designed and synthesized a 20nt telomerase-antisense DNA targeting telomerase template and observed its influence on the telomerase activity of hepatoma cells. H-E staining, flow cytometry, and DNA agarose electrophoresis were used to study the preventive effect of telomerase-antisense DNA on hepatoma cells apoptosis induced by arsenic trioxide. Flow cytometry was used to detect the expression of Fas, Fas-L, and bcl-2. Results: Telomerase-antisense DNA (5 ?mol/L) effectively inhibited the telomerase activity of hepatoma cells after 24 hours (P
10.Establishment and biological characteristics of a platinum-resistance nude mouse model in epithelial ovarian cancer
Lijun SHI ; Hongjing YU ; Wei ZHANG ; Li LI ; Qi WANG
Chinese Journal of Obstetrics and Gynecology 2014;(7):523-530
Objective To establish a platinum resistance nude mice model of epithelial ovarian cancer (EOC) and investigate its resistance to cisplatin (DDP) biological characteristics, so as to provide evidences for exploring chemoresistence mechanisms and screening for reversal targets in vivo micro-environment. Methods The resistance model was produced by repeating a crossover subcutaneous injection of human ovarian cancer SKOV3 cells labelled green fluorescent protein(GFP) and transplatation of tumor fragment into nude mice. Two kinds of cancer cell lines of SKOV3/DDPⅠand SKOV3/DDPⅡwere induced with acquired resistence to DDP. The chemosensitivities of EOC cells to DDP were tested and half maximal inhibitory concentration(IC50) was measured by methyl thiazolyl tetrazolium (MTT) and flow cytometry (FCS). Dynamic analysis among the concentration of DDP treatment and cell apoptosis, cell cycle phase distribution and intracellular DDP concentration. The expression of PTEN, STAT5, XIAP, BRCA1 and MDR1 were examined by real time quantitative reverser transcription PCR (qRT-PCR) in vivo. Results IC50 value of cisplatin for SKOV3/DDPⅡ were 2.83 ± 0.12 and 3.82 ± 0.19 folds than those for SKOV3/GFP by MTT and flow cytometry, separately. SKOV3/DDPⅠwere 2.20±0.16 and 3.40±0.20 folds. The apoptosis rate of SKOV3/DDPⅡ and SKOV3/DDPⅠ were decreased significantly at 29.7 and 39.6μmol/L DDP when treatment for 36 hours,which were lower than that of SKOV3/GFP cells [(57.0±1.4)%vs (37.6 ± 4.36)%vs (83.1 ± 2.71)%,P=0.024;(74.4 ± 2.3)%vs (50.5 ± 3.4)%vs (87.4 ± 4.0)%,P=0.001]. SKOV3/DDPⅠ and SKOV3/DDPⅡ was positively related with cisplatin processing time. Intracellular DDP accumulation of SKOV3/DDPⅡand SKOV3/DDPⅠwere lower than SKOV3-GFP in dynamic processes(P<0.05). Besides intracellular DDP accumulation of SKOV3/DDPⅡ also lower than SKOV3/DDPⅠin dynamic processes (P<0.05). Transplanted tumor of SKOV3/GFP appeared organelle degradation and nuclear membrane imcompleted after five times DDP injection with concentration of 4 mg/kg. SKOV3/DDPⅡand SKOV3/DDPⅠdid not generate these phenomenon untill eighth DDP injections with concentration of 4 mg/kg. STAT5 and BRCA1 of SKOV3/DDPⅡwere increased with DDP treatment at concentration of 4 mg/kg. Expression of XIAP from SKOV3/DDPⅡwas positive correlated with injection times. STAT5,XIAP and BRCA1 of SKOV3/DDPⅡwere up-regulated 3.86,28.1 and 14.6 folds than those in SKOV3/GFP cells after eighth DDP treatment, separately. While PTEN of SKOV3/DDP Ⅱ was decreased 3.77 folds. Conclusions We have successfully established platinum-resistent EOC mice model,which provides a new platform for further study on chemoresistant reversal and individualized clinical treatment. The results shown that potential mechanisms of SKOV3/DDPⅡDDP-resistance included over-expressed BRCA1 gene may be promote DNA damage repair, elevate XIAP gene to decrease cell apoptosis,up-regulated STAT5 gene and decrease PTEN gene to stimulate proliferation.