Acupuncture Points ; Acupuncture Therapy ; Female ; Humans ; Male ; Maternal-Fetal Exchange ; Pregnancy ; Pregnancy Complications ; therapy

China ; Health Knowledge, Attitudes, Practice ; Humans ; Occupational Diseases ; prevention & control ; Occupational Exposure ; prevention & control ; Occupational Health ; statistics & numerical data ; Rural Population ; statistics & numerical data

China ; Health Education ; Health Knowledge, Attitudes, Practice ; Humans ; Occupational Diseases ; prevention & control ; Occupational Health ; Rural Population ; Sampling Studies ; Transients and Migrants

OBJECTIVE To study the epidemiology of drug-resistant and antiseptic-resistant genes of(productive)(enzyme) in meticillin-resistant coagulase negative Staphylococcus(MRCNS).METHODS mecA Gene of the(drug-)resistant ?-lactam,aac(6′)/aph(2″) and aph(3′)-Ⅲ genes of aminoglycoside-modifying enzyme(AME) and(ermA/B/C) genes of erythromycin methyltransferase were detected by polymerase chain reaction(PCR) in 40 MRCNS strains.RESULTS From them there were 39 strains with mecA gene,32 strains with aac(6′)/aph(2″) gene,15 strains with aph(3′) -Ⅲ gene,30 strains with ermA/B/C gene,2 strains with tetM and 6 strains with qacA/B gene.There were 26 strains(65.0%) simultaneously with mecA,aac(6′)/aph(2″)and(or) aph(3′)-Ⅲ and ermA/B/C genes in MRCNS.CONCLUSIONS The drug-resistant rate is higher in MRCNS.There are more than half tested strains simultaneously with 3 to 4 drug-resistant genes.

Objective To detect the important parameters of three dimensional structure of the aortic annulus by using real time three dimensional transesophageal echocardiograhy (RT 3D TEE) and analyse morphological and physiological characteristics , in order to establish simplified model of aortic valve and provide references for designing aortic annulus .Methods A total of 95 ca‐ses of healthy individuals carrying out RT 3D TEE were selected ,and the images of total volume of aortic root were collected .The important parameters of three dimensional aortic valve in four cardiac cycles ,including sinus tube junction(S) ,distance between ad‐jacent valve junction(L ,R and N) ,diameter of the circumcircle of triangle(J) ,aortic annulus height(H) ,long diameter(A) ,short di‐ameter(B) and eccentricity ratio (B/A) of the aortic valve ,were measured by using the QLAB7 .0 software .In the 3D imaging ,the extending relationship between the bottom of aortic valve and aortic annulus was analysed ,the structural characteristics of the aortic valve were simplified ,and the model was established .Results The values of the parameters measured at the end‐diastole were as follows :sinus tube junction (26 .3 ± 3 .3)mm ,three‐dimensional aortic annulus height(12 .4 ± 1 .9)mm ,aortic valve long diameter and short diameter (25 .9 ± 2 .7)mm and (19 .8 ± 2 .0)mm ,adjacent valve junction distance(L ,R and N) all was(21 .6 ± 2 .5)mm . These three structures approximately formed an equilateral triangle .The values of the adjacent cycle time ,the change of sinus tube junction ,distance between adjacent valve junction ,aortic annulus height and long diameter were compared ,and there was no statisti‐cal significant differences(P>0 .05) ,while statistically significant differences were found in the aortic valve short diameter and long short diameter between those at the end‐diastole and those at the medium‐systole(P<0 .01) .Conclusion RT 3D TEE can accu‐rately measure the size of each part of the aortic valve and display its shape and change ,which contribute to simplifying and recon‐structing the aortic annlus .

Objective To explore the features of clinical manifestations and electrophysiology of spinal muscular atrophy(SMA) in children.Methods The clinical features and laboratory data were analyzed in 32 children with SMA,electromyography tests were carried out in 28 patients and the muscle biopsy were performed in 24 cases.Results The 32 cases were subdivided into 3 clinical groups,15 cases were SMAⅠ,12 cases were SMAⅡ,5 cases were SMAⅢ.They were all characterized by progressive muscle weakness associated with hyptonia and atrophy.The clinical distinction between SMAⅠto SMAⅢ reflected different age of onset and disease severity.All cases of SMAⅠhad symptoms of respiratory disability,only 1 case of SMAⅡ had paradoxical breathing and none of SMAⅢ had similarly symptoms.Electromyographic studies showed a pattern of denervation with no sensory involvement.The rate of spontaneous potential was 87%,with gentle strain,the duration of motor unit was extended(30%-150%) and the amplitude of it was increased(90%-450%),the motor nerve conduction velocity was reduced slightly in 28% patients.The muscle biopsy provided evidence of skeletal muscle denervation with groups of atrophy.Conclusions The clinical features,the changes of electromyography and the muscle biopsy are valuable for diagnosis of SMA.Respiratory management will prolong survival and improve the quality of life for these patients.

China ; epidemiology ; Humans ; Occupational Diseases ; epidemiology ; prevention & control ; Occupational Exposure ; prevention & control ; statistics & numerical data ; Rural Population ; Transients and Migrants

Objective To observe the effect of electromyographic biofeedback therapy and kinesitherapy on patients with radial nerve injury.Methods 82 patients with radial nerve injury were divided into control group (n=41) and treatment group (n=41). The control group was treated with medcine and acupuncture, while the treatment group received electromyographic biofeedback therapy and kinesitherapy in addition. The radial nerve function was assessed and analyzed by electromyogram (EMG) and motor conduction velocity (MCV) before and after treatment. Results After 12 weeks of treatment, limb motor function, EMG and MCV were better in the treatment group than in the control group (P<0.05). Conclusion Electromyographic biofeedback therapy and kinesitherapy can effectively improve the limb motor function of patients with radial nerve injury

Objective: By observing the differences in Western Ontario and McMaster Universities osteoarthritis index (WOMAC) and visual analog scale (VAS) scores in moxibustion treatment for moderate-to-severe primary knee osteoarthritis (KOA) with moxa of different storage years (3-year moxa and 1-year moxa from Qichun, Huanggang City, Hubei Province, China) through a randomized clinical trial, to objectively evaluate the differences in therapeutic efficacy of moxibustion with moxa of different storage years. Methods: A total of 63 patients with moderate-to-severe KOA who met the inclusion criteria were randomized into moxibustion group 1 and moxibustion group 2 by central randomization method, with 32 cases in moxibustion group 1 and 31 cases in moxibustion group 2. Moxibustion group 1 was treated with moxa stored for 3 years, and moxibustion group 2 was treated with moxa stored for 1 year. Dubi (ST 35), Neixiyan (EX-LE 4) and Heding (EX-LE 2) were selected in both groups, and the treatment lasted 20 min per time, 3 times a week. The immediate efficacy was compared after 6 times of treatment, and long-term efficacy was compared at follow-up 4 weeks after the end of treatment. Results: During the treatment, there were 2 dropouts in moxibustion group 1, and 1 dropout in moxibustion group 2. The total effective rate in the two groups was 83.3％ and 60.0％, respectively. Followed up at 4 weeks after the end of treatment, the total effective rate in the two groups was 80.0％ and 66.7％, respectively. There were no statistical differences between the two groups (both P>0.05). After treatment and 4 weeks after the end of treatment, the WOMAC and VAS scores in both groups decreased significantly compared with those before treatment (all P<0.01); the scores of stiffness item of WOMAC in moxibustion group 1 were lower than those in moxibustion group 2 (both P<0.05); there were no statistical differences in the scores of pain item and dysfunction item of WOMAC, and VAS scores between the two groups (all P>0.05). Conclusion: Moxibustion with moxa of different storage years (stored for 3 years and 1 year) both can improve the pain, stiffness and motor function in patients with moderate-to-severe KOA. While moxa stored for 3 years has a better therapeutic efficacy in improving stiffness of the knee joint than that stored for 1 year.

Aim To investigate the effects of dual COX-2/5-LOX inhibitor darbufelone on the inhibition of gastric adenocarcinoma in vitro.Methods MTT assay was used to detect the inhibition of gastric adenocarcinoma cell line SGC-7901 by darbufelone in different concentrations and at different times.TUNEL staining was used to evaluate the apoptosis of SGC-7901 cells.The changes of 5-LOX and COX-2 mRNA expression were studied by reverse transcription polymerase chain reaction.5-LOX and COX-2 protein expressions were analyzed by immunocytochemistry in SGC-7901 cells.Results darbufelone could decrease the proliferation significantly in a dose-dependent and time-dependent manner in SGC-7901 cells.After treating SGC-7901 with darbufelone at concentration of 1.5?10-5,1.0?10-5,5?10-6 mol?L-1 for 72 hs,apoptosis index(%) of SGC-7901 was(30.3?2.1)%,(23.0?2.0)%,(15.0?1.5)%,respectively,which was significantly higher than(0.6?0.1)% that in control group(P