Objective To investigate the diabetes self-management (DSM) ability and influencing factors in patients with type 2 diabetes in Kunming. Methods A face to face questionnaire survey was conducted on type 2 diabetes patients admitted in the 1st Affiliated Hospital of Kunming Medical University from November 2010 to April 2012. Diabetes self-management instrument ( DSMI) was used to test self-management behavior, and psychological status was assessed by Problem Areas in Diabetes (PAID-5).Results 300 patients participated in the survey.The participant population consisted of 120 males (41.8%) and 168 females (58.2%) .A total of 190 patients (68%) suffered from DM related psychological problems.The mean DSM score was 95.23±20.6, and mean scores for the domain of integration DM care into one’s life, self-regulations, interaction with health professionals,self-blood monitoring glucose,and adherence to recommended regimen were 28.11±6.15,25.22± 5.67,23.06±6.41,10.98±2.96,and 8.75±2.21,respectively. There was no significant difference in the mean score between males and females (P>0.05) .Patients with psychological problems had lower scores in five DSM dimensions than those without psychological problems (P<0.05) . The result of multiple regression showed higher level blood glucose was negatively associated with DSM score, whereas patients with higher income was associated with a higher DSM score (P<0.05) .Conclusions The DSM situation is not good among patients with type 2 diabetes in Kunming, and most of the patients suffer from psychological problem. It is necessary to enhance the effective health education among diabetic patients.

Adolescent ; Adult ; Follow-Up Studies ; Fracture Fixation, Internal ; methods ; Humans ; Male ; Recovery of Function ; Tibial Fractures ; diagnostic imaging ; physiopathology ; surgery ; Tomography, X-Ray Computed ; Young Adult

Mice was stimulated by peptidoglycan from Lactobacillus sp and detect cytokines production。It was found that PG induced the production of inflammatory cytokines (IL-1,TNF-? in peritoneal macrophages,IFN-? in spleen cell)and did not induce the IL-2 production in spleen cell. Affymetrix MOE430A genechip was used to analyze changed gene expression of immune cells. It was found that expression of cytokines and related genes were changed under peptidoglycan administration. This might induced by activation of TLR-NF-?B signal pathway.

Adolescent ; Adult ; Female ; Fracture Fixation, Internal ; methods ; Humans ; Male ; Middle Aged ; Tibial Fractures ; surgery ; Treatment Outcome ; Young Adult

Objective To analyze the occurrence characteristic and preventive measures of adverse drug reactions (ADR) caused by monascus single drug preparations.Methods To retrieve Chinese CNKI,Wanfang database and VIP database,as of May 2017.Office software was used to analyze the adverse drug reactions of monascus single drug.Results A total of 13 articles,including 13 patients,were included.Adverse drug reactions of monascus single medicine preparations involved the system and (or) the main organ for musculoskeletal system damage (38.46％),followed by hepatobiliary system damage (23.08％),and the urinary system damage(15.38％).Conclusion The drug production enterprises should strengthen the revision of drug adverse drug reactions of the drug in clinical application,and attention should be paid to the occurrence of adverse drug reactions of monascus monotherapy drugs in clinical use.Besides the understanding of adverse drug reactions and medication guide should be strenthened to avoid the occurrence of serious adverse drug reactions.

Eight distinct bacteria were isolated form diseased mycelia of the edible mushroom, Pleurotus eryngii. 16S rDNA sequence analysis showed that the isolates belonged to a variety of bacterial genera including Bacillus (LBS5), Enterobacter (LBS1), Sphingomonas (LBS8 and LBS10), Staphylococcus (LBS3, LBS4 and LBS9) and Moraxella (LBS6). Among them, 4 bacterial isolates including LBS1, LBS4, LBS5, and LBS9 evidenced growth inhibitory activity on the mushroom mycelia. The inhibitory activity on the growth of the mushroom fruiting bodies was evaluated by the treatment of the bacterial culture broth or the heat-treated cell-free supernatant of the broth. The treatment of the culture broths or the cell-free supernatants of LBS4 or LBS9 completely inhibited the formation of the fruiting body, thereby suggesting that the inhibitory agent is a heat-stable compound. In the case of LBS5, only the bacterial cell-containing culture broth was capable of inhibiting the formation of the fruiting body, whereas the cell-free supernatant did not, which suggests that an inhibitory agent generated by LBS5 is a protein or a heat-labile chemical compound, potentially a fungal cell wall-degrading enzyme. The culture broth of LBS1 was not inhibitory. However, its cell-free supernatant was capable of inhibiting the formation of fruiting bodies. This indicates that LBS1 may produce an inhibitory heat-stable chemical compound which is readily degraded by its own secreted enzyme.
Agaricales ; Bacillus ; Bacteria ; DNA, Ribosomal ; Enterobacter ; Fruit ; Moraxella ; Ostreidae ; Pleurotus ; Sequence Analysis ; Sphingomonas ; Staphylococcus

BACKGROUND AND OBJECTIVEMultinodular hepatocellular carcinoma(HCC) might originate from multicentric occurrence (MO) or intrahepatic metastasis(IM). This study was to find out proteins which play important roles in clonal origin of multinodular hepatocellular carcinoma bt screening the differentially expressed proteins between the MO and IM tissues using comparative proteomic analysis.

METHODSTotal protein extracted was separated by two-dimensional gel electrophoresis. Comparative analyses of the 2-DE protein patterns between the two groups were carried out using computerized imaging techniques. Proteins exhibiting significant alternations were subsequently isolated and identified by mass spectrometry.

RESULTSA total 1025+/-52 and 900+/-98 spots were detected in the protein profile in IM and MO, respectively. Twenty-five protein spots were statistically different at expression levels between the two groups. Twenty of them were identified by MALDI-TOF-MS and bioinformatics.

CONCLUSIONSThe protein profile of MO HCC tissues is different from that in IM HCC tissues. The twenty differentially expressed proteins might play a key role in the carcinogenesis and progression of multinodular HCC. These newly identified proteins might be potential and valuable biomarkers for identifying the multinodular HCC of clonal origin.

Adult ; Carcinoma, Hepatocellular ; metabolism ; pathology ; Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Liver Neoplasms ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Metastasis ; Neoplasms, Multiple Primary ; metabolism ; pathology ; Protein Array Analysis ; Proteomics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Background and objective Lung cancer is the most common malignancy and screening can decrease the mortality. High quality screening guideline is necessary and important for effective work. Our study is to review and evalu-ate the basic characteristics and methodology quality of the current global lung cancer screening guidelines so as to provide useful information for domestic study in the future.Methods Electronic searches were done in English and Chinese databases including PubMed, the Cochrane Library, Web of Science, Embase, CNKI, CBM, Wanfang, and some cancer offcial websites. Articles were screened according to the predeifned inclusion and exclusion criteria by two researchers. hTe quality of guidelines was assessed by AGREE II.Results At last, a total of 11 guidelines with methodology were included. hTe guidelines were is-sued mainly by USA (81%). Canada and China developed one, respectively. As for quality, the average score in the “Scale and objective” of all guidelines was 80, the average score in the “Participants” was 52, the average score in the “rigorism” was 50, the average score in the “clarity” was 76, the average score in the “application” was 43 and the average score in the “independence”was 59. hTe highest average score was found in 2013 and 2015. Canada guideline had higher quality in six domains. 7 guidelines were evaluated as A level.Conclusion hTe number of clinical guidelines showed an increasing trend. Most guidelines were issued by developed countries with heavy burden. Multi-country contribution to one guideline was another trend. Evidence-based methodology was accepted globally in the guideline development.

Objective:To study the value of micro-CT in monitoring the dynamic evolution of acute pulmonary tuberculosis in mice.Methods:Establish the murine tuberculosis model of C57BL/6J in intranasal as the experiment group.The control group was set at the SPF level.Micro-CT scans were undertaken 1,4,8,12 weeks later,respectively.And then the mice were sacrificed and the lung tissue was taken for pathological analysis.Objective:In the experiment group,nine mice died and three were negative.So,totally 28 mice were enrolled.There were 7,6,8,7 mice in each subgroup,respectively.One week after infection,ground glass opacity(GGO)was 71.4％and consolidation was 57.1％in the experiment group.Corresponding pathology showed alveolar exudate and alveolar septum widened.Four weeks after infection,the imaging findings included GGO and patchy opacities(in 66.7％)in the experiment group.Microscopically,alveolar effusion and consolidation,with epithelioid cells,multinucleated giant cells and foam cells were found.Eight weeks after infection,nodules,air bronchogram and consolidation had equal proportion in the experiment group.Microscopically,it showed consolidation and tuberculosis nodules.12 weeks after infection,nodules had the highest proportion,about 57.1％in the experiment group.Microscopically,the nodules showed granulomas.Conclusions:The images of acute pulmonary tuberculosis murine model can reflect the pathological changes,which can be used to simulate the course of human pulmonary tuberculosis and evaluate the disease process.

OBJECTIVE: To observe the impact of acupuncture on myofascial trigger points(MTrPs),low-frequency electrical stimulation and manipulative stretch reduction on sacroiliac joint on perinatal period separation of symphysis pubis.METHODS: Totally 100 cases of perinatal separation of pubic symphysis in Huai'an Women and Children Health Hospital from January 2013 to July 2016 were selected.They were randomly divided into comprehensive treatment group,electrical stimulation group,manipulative reduction group,pelvic belt group and control group,20 cases in each group.The comprehensive treatment group was given acupuncture on myofascial trigger points,low-frequency electrical stimulation and manipulative stretch reduction on sacroiliac joint,the other three treatment groups were treated with their respective monotherapy,and the control group only received the brake observation.The NRS pain score and clinical efficacy were compared among the groups.RESULTS: Six days after treatment,18 cases,7 cases,4 cases,0 case and 0 case were cured in the five groups,respectively.Six weeks after treatment,20 cases,13 cases,11 cases,4 cases and 0 case were cured in the five groups,respectively.Six months after treatment,20 cases,18 cases,15 cases,13 cases and 9 cases were cured in the five groups,respectively.The treatment effect of the comprehensive group was the best,followed by the electric stimulation group,and then manipulation reduction group.CONCLUSION: acupuncture on myofascial trigger points and electrical stimulation combined with manipulative stretch reduction can not only quickly relieve the pain of patients in a short time,but also quickly make the pubic symphysis form return to normal.It has a high treatment efficiency and is less painful.So it has a high clinical value.