The Weibel-Palade-like body were found in the cytoplasm of the growth hormone and follicullar - stellate cells of rabbit adenopypophsis. The body is surrounded by a single unit membrane. In this body, there are numerous of microtubules which can be divided into two groups depending upon their diameters.Yet, it is uncertain what the virtual function is and where the bodies come from. However, according to the experimental observations, it is suggested that the body serve as a mechanical power device which has relations with the regional distribution of the organelles. The intranuclear microtubules and intranuclear tubules were also found in the growth hormone cells. The diameters of these intranuclear microtubules range from 25 - 30 nm, and the intranuclear tubule about 78 nm. The fomer seems to be connected with the heterochromatin granules, and may be. a transitional pattern from heterochromatin to euchromatin, but their importance remain to be clarified.

The ultrastructural changes in growth hormone (GH) cells of rabbit adeno-hypophysis are determined quantitatively with stereological method after burn injury. Blood sugar levels are concurrently measured. The results show: the volume fraction of Golgi complex, mitochondria and rough endoplasmic reticulum shows a 2-phase curve after burn, and it may be divided into 3-periods stress, modulation and functional activation and recovery; the rate of maturation of secretory granules is increased; a close relation between the blood sugar and some activities of GH cell secretion after burn presents; the autocrine shows a negative feedback to the exocytosis in the control group,and a positive feedback in burned ones; the newly formed mitochondria may be originated from the new Golgi complex from the reutilization of plasma components.

Objective To evaluate the importance of the prenatal diagnosis to fetus with mild ventriculomegaly ( MVM) in pregnancy outcome and prognosis. Methods The ultrosonographic results of 51 pregnant women who were admitted to our hospital were collected in this study. In each case,a thorough evaluation of fetal anatomy was performed,karyotyping or MRI was done if needed,and follow-up was done un-til afterbirth. Results In 51 cases of lateral ventriculomegaly fetal:ultrasonography indicated there were 26 fetuses with mild ventriculomega-ly. The incidence of MVM complicated with other abnormities was 46. 0%,and that complicated with chromosome disorder was 11. 5%. Con-clusion Ultrasonography plays an important role in the detection of MVM. Chromosome analysis should be carried out for elder pregnant women when their fetuses are diagnosed as MVM and other complicating abnormities.

Food Contamination ; prevention & control ; Public Health Practice

Objective To evaluate the sonography features of heptaobiliary involvement of pediatric Langerhans′cell histiocytosis ( LCH) . Methods Nine pediatric patients with LCH confirmed by the clinical presentation ,biochemical tests and pathologic results of our hospital were retrospectively analyzed . Results Eight patients were seen in multi‐system LCH ,while one patient only had liver involved . All patients showed varying hepatomegaly with multiple distributed lesions in ultrasound images . According the lesion distribution features ,they could be classified into three types:central type(6/9) ,peripheral type(1/9) and diffuse type(2/9) . In ultrasonography ,they manifested as linear lesions alongside the central portal veins , multi‐nodular and randomly distributed lesions in liver parenchyma ,or both of them . Eight bile‐duct involved patients behaved with unevenly thicked bile‐duct walls ,stenosis or dilated bile‐ducts ,and even sclerosing cholangitis , some of them with cholelithiasis . Conclusions Pediatric LCH patients with heptobiliary involved are seemed mainly in multi‐system LCH ,while it is rare but possible that pathological Langerhans′cell only infringe on liver and bile‐ducts . The ultrasound imaging findings of pediatric LCH liver infiltration are with characteristic and are helpful for the early diagnosis and clinical procedure .

Objective To explore the imaging features of pediatric splenic primary solitary tumor. Methods The medical imaging appearances of 6 children with splenic primary solitary tumor conifrmed by surgery and pathology from January 2010 to August 2015 were retrospectively studied. The related literatur were reviewed. Results In 6 children, 2 children of cystic lesions, ultrasound showed anechoic mass, CT scan showed low density mass, without change after contrast, pathologically conifrmed as congenital cyst;2 children’s ultrasound showed solid echo, color Doppler ultrasound explored the blood lfow signals, contrast CT showed the lower strength around the splenic parenchyma, pathologically conifrmed as splenic lymphangioma;one child’s unltrasound showed echo mass with rich signals of blood lfow, MRI scans showed T1WI, T2WI and other signals of lumps, with little homogenity after contrast, pathologically conifrmed as splenic hamartoma;one child’s ultrasound cannot distinguish tumor boundaries, contrast CT scan showed concentric filling reinforcement, pathologically confirmed as hemangioma. Conclusion Splenic primary solitary tumor in children is rare. There are no speciifc clinical characteristics. The image ifndings can deifne the locations and help the differential diagnosis. The ifnal diagnosis depends on pathology.

Cardiac toxicity is found in frequently used chemotherapeutic agents.There are many factors related to the cardiac toxicity caused by chemotherapeutic agents.The common cardiovascular complications include heart failure,ischemia,hypertension,hypotension,edema,QT prolongation,pleural effusion,pericardial effusion,bradyarrhythmia and thromboembolism.It is necessary to monitor the left ventricular function before and after chemotherapy and take effective measures to protect myocardium.

Object To explore the beneficial effect of phytoecdysone (EDS) on myocardial infarction and its mechanism of action Methods Rat myocardial infarction model was prepared by ligating the left anterior descending coronary artery, and EDS was injected ip for seven consecutive days Serum creatine phosphokinase (CPK) glutamic oxalacetic transaminase (GOT), lactic dehydrogenase (LDH) activities, infarct size(IS), coronary blood flow, capillary vessel density and vascular endothelial growth factor (VEGF) expression were determined Results 0 5, 5, and 50 mg/kg of phytoecdysone were able to effect the activities of serum CPK, GOT, LDH in a dose depending manner with an optimal effect for improving cardiac zymogram at the dose of 5 mg/kg ip At this dosage EDS can markedly reduce IS, increase coronary blood flow, capillary vessel density and the expression of VEGF Conclusion ESD can alleviate myocardial infarction symptoms The mechanism of such beneficial effect may due to its ability to promote VEGF expression regeneration of capillary vessels and increase coronary blood flow

Objective To evaluate the influence of duration of laser irradiation on histomorphometric meas- urements in experimentally tenotomized and repaired rabbit Achilles tendons and to explore the best irradiation time. Methods A total of 20 male New Zealand rabbits aged 10-12 weeks were used and randomly divided into 4 groups:a control group and three experimental groups.All the animals underwent surgical excised and then repair of their Achillis tendon.The animals in the control group were then treated with sham laser irradiation,while those in the three experimental groups were treated with 10,20 and 30 minutes of He-Ne laser irradiation(632.8 nm, 18.9 mW)daily,respectively,for 14 days.On the 28th day after surgical operation,the animals were sacrificed and their Achilles tendons were sampled.HE stain and Van Geison stain were used to observe morphometric changes of tendons.The SDS-PAGE electrophoresis method and CS-930 photodensity scan instrument were employed to measure the content of typesⅠandⅢcollagen.Results it was shown that laser irradiation enhanced cell proliferation,cel- lular content,granulation tissue formation and collagen deposition in laser-treated tendons,especially in those irradia- ted for 20 minute daily,as compared to the control group.TypeⅠand typeⅢcollagen levels were significantly in- creased at the 28th day in the healing tendons and the ratio of collagenⅢtoⅠincreased in all the 3 experimental groups,and the increase of both collagen content and ratio of collagen typeⅢtoⅠwas significantly greater in those ir- radiated 20 minutes daily(P

Objective: To identify the mutation points of Cu/Zn superoxide dismutase(SOD1) gene in an amyotrophic lateral sclerosis(ALS) family with a unique phenotype,and to compare the value of single strand conformation polymorphism(SSCP) and denaturing high performance liquid chromatography(DHPLC). Methods: Five exons of SOD1 gene were amplified by PCR. The difference of these products were analyzed by PCR-SSCP and DHPLC.DNA sequencing was used to examine the mutation. Results: ①Mutations were found in exons 2 and 5 in several family members.DNA sequencing revealed that a base pair insertion occurred in the codon area of exon 2 and in the non-codon area of exon 5.②The results of DHPLC tests proved double peaks in one member with ALS symptoms(Ⅲ1),which indicated the possibility of mutation in SOD1 exon 4.DNA sequencing revealed that there was a heterozygote,with a mutation of GAA to GGA in exon 4 in the member with double peak. Conclusion: ①The mutations in exons 2,4,5 were proved.Insertion of exon 2 may be responsible for the disease of the ALS family in Chongqing.②Compared with PCR-SSCP,DHPLC technique has been proven to be a rapid and reliable method for screening mutation site in large samples.