Objective To study the expression and cellular localization of STAT3 and SOCS-3 in the motor neurons of normal rat spinal cord. Methods Immunocytochemistry and morphometry methods were used in the present study. Results STAT3 immunocytochemical staining was mainly distributed in the cytoplasm of the motor neurons of the ventral horns, SOCS-3 immunoreactive products were extensively distributed in the neurons of the ventral and dorsal horns, glia and fibers of the spinal cord. In the ventral horn, SOCS-3 immunoreactivity was found in the nuclei and/or cytoplasm of the motor neurons.Conclusions STAT3 and SOCS-3 are extensively distributed in the normal adult rat spinal cord and SOCS-3 is existence in the forms of nuclear or cytoplasmic protein.

Objective To analyze the literature distribution and research status of osteoporosis in the elderly published in Chinese Medical Association journals.Methods The literatures of elderly osteoporosis published in Chinese Medical Association journals from 2008 to 2013 were collected.And the publication year,distribution,content,institution and region of the literatures were analyzed.Results From 2008 to 2013,there were 76 articles about osteoporosis in the elderly published in 22 kinds of journals of the Chinese Medical Association.Among these journals,the Chinese Journal of Geriatrics-published elderly osteoporosis articles occupied the most number [28 (36.8％)].There were 21 articles published mostly in 2013 (27.6％),secondly in 2009 (17 articles,22.4％) and 2012 (12 articles,15.8％).The province or city that published more than 10 articles was Beijing (18 articles,23.7％),Zhejiang (13 articles,17.1％),Shanghai (10 articles,13.2％) and Guangdong (10 articles,13.2％).The institution that published most articles was Beijing Jishuitan Hospital (4articles,5.3％).The main contents of these literatures included the influencing factors,diagnosis,treatment strategy,bone metabolism and surgery treatment for elderly osteoporosis.Conclusions Chinese Medical Association journals are the important source to acquire osteoporosis literature for the medical workers.The distribution of journal is relatively concentrated,but the distribution of authors,institution is dispersed.Chinese Journal of Geriatrics publishes the most number of articles.The research of osteoporosis has attracted more attention.

To investigate the effects of ischemia-reperfusion on the levels of nitric oxide and nitric oxide synthase isoforms (nNOS and iNOS), rat organotypic hippocampus slice were cultured in vitro and subjected to ischemia by oxygen-glucose deprivation (OGD) for 30 min and then placed in the normal culture condition. The ischemia-reperfusion produced a time-dependent increase in nitrite levels in the culture medium. Reverse transcriptional-polymerase chain reaction showed augmented levels of mRNA for both nNOS and iNOS when compared with control at 12 h and remained increase at 36 h after OGD (P < 0.05). The protein levels of both nitric oxide synthase isoforms increased significantly as determined by Western Blot. OGD also caused neurotoxicity in this model as revealed by the elevated lactate dehydrogenase (LDH) efflux into the incubation solution. The results suggest that organotypic hippocampus slice is a useful model in studying ischemia-reperfusion brain injury. NO and NOS may play a critical role in the ischemia-reperfusion brain damage in vitro.
Animals, Newborn ; Cell Hypoxia ; Hippocampus/cytology ; Hippocampus/*metabolism ; Nitric Oxide/*metabolism ; Nitric Oxide Synthase Type I/*metabolism ; Nitric Oxide Synthase Type II/*metabolism ; RNA, Messenger/metabolism ; Rats, Sprague-Dawley ; Reperfusion Injury/*metabolism ; Tissue Culture Techniques

Objective To evaluate the short -term efficacy and adverse reactions of gemcitabin combined with nedaplatin to patients with metastatic triple -negative breast cancer after taxane and/or anthracycline treatment. Methods Thirty -three patients with anthracycline and/or taxane -resistant metastatic triple -negative breast cancer received gemcitabin and nedaplatin regimen:gemcitabin 1 000 mg/m2,d1,8 days,intravenous;nedaplatin 80mg/m2,d1,intravenous.Treatments were repeated every 21 days and response rate was evaluated every two cycles. Results Of 33 patients, complete remission ( CR) in 1 case ( 3.03%), partial remission ( PR) in 13 cases (39.39%),stable disease(SD) in 11 cases(33.33%),progressive disease(PD) in 8 cases(24.24%),objective response rate was 42.42%.The main adverse reaction was bone marrow suppression and gastrointestinal reaction. Conclusion Combination therapy of gemcitabin and nedaplatin is an effective and well tolerated rescue regimen in anthracycline and/or taxane -resistant metastatic triple -negative breast cancer patients.

Objective The objective of this study was to investigate the expression and clinical significance of transcription factors Sp1 and Survivin in breast cancer and to further analyze their correlation.Methods Seventy cases of breast cancer patients were diagnosed as breast cancer from June 2013 to June 2015.The expression of Sp1 and Survivin in 70 cases of breast cancer and 20 cases of adjacent normal tissues were detected by immunohistochemical staining.The relationship was detected between the protein expression and the clinicopathological parameters of breast cancer.Results The positive rates of Sp1 and Survivin in breast cancer were 71.43% and 78.57%,respectively.The positive rates of Sp1 and Survivin were 30% and 10% in adjacent normal breast tissues.They showed a significant difference(P<0.05).In breast cancer tissues,there were no differences in the lymph node metastasis,TNM stage and vessel infiltration(P>0.05).There were also no differences in the age,tumor size and histological grade of patients with breast cancer(P>0.05).In breast cancer tissues,there was a significant positive correlation between Sp1 and Survivin expression(r=0.517,P<0.01).Conclusion The transcription factors Sp1 and Survivin are closely related to the pathogenesis of breast cancer,which may serve as an adjunctive index for the diagnosis of breast cancer.It has certain guiding value for clinical diagnosis,treatment and prognosis of breast cancer.

AIM: To observe the effect of mouse Sim2 (mSim2) eukaryotic expression vector transfection on the cell cycle in PC12 cells in vitro and to explore the role of Sim2 in the pathogenesis of Down syndrome. METHODS: The full open reading frame of mSim2 was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into the vector pcDNA3. Then the constructed pcDNA3-Sim2 vectors were transiently transfected into PC12 with Lipofectamine~ TM . The expression of mSim2 was detected by RT-PCR. The effect of mSim2 on the cell cycle was observed by flow cytometry. RESULTS: The eukaryotic expression vector mSim2 was successfully constructed. There was notable expression of mSim2 in the cells transfected with pcDNA3-Sim2. There were more cells in G_0/G_1 phase in the pcDNA3-Sim2 transfected cells than that in the control (P

Objective To study the basic expression and cellular localization of SOCS-3 in normal rat retina. Methods Neuro-immunocytochemistry techniques were used. Results SOCS-3 positive cells were widely distributed in the ganglion cell layer (GCL) and inner nuclear layer (INL) in the retina. In the GCL, SOCS-3 immunoreactivity was mainly in the neuclei of the ganglion cells.Some of the SOCS-3 positive cells in INL were M?ller cells.Conclusion Basal expression of SOCS-3 is widely present in the neurons and glia in normal adult rat retina and mainly in the form of nuclear protein.

Objective To analyze the citation of papers published in Chinese Journal of Geriatrics from the year 2008 to 2012,and evaluate the quality and academic level of the journal.Methods Based on the information from Wanfang database,bibliometric analysis was conducted to analyze number of cited papers,citation frequency,citation time distribution,authors,funds supports etc,and immediacy index and citation half-life were calculated.Results A total of 1567 papers were published in the journal from the year 2008 to 2012,and 377 articles were cited for 669 times with citation rate of 24.06％.The average number of cited times was 1.77,and the top value per article cited was 18 times.There were 12 authors whose articles were cited 6 and over times,and 11 affiliations whose articles were cited more than 10 times.Among 377 papers,105 papers (27.85％) were supported by funds,among which the majority was provincial fund (36 papers) and national fund (35 papers).Immediacy index from the year 2008 to 2012 were 0.042,0.032,0.019,0.067 and 0.006,respectively,and the citation half-life was 1.71 in the year 2012.Conclusions Articles published in Chinese Journal of Geriatrics have high-quality and can reflect the development and research progress in geriatric medicine.The journal is one of the most important information resources in geriatric academic field in China.

Objective To explore the distribution and research status of literatures on Alzheimer disease (AD) published recently,to compare the differences in the research papers on AD between China and foreign countries,and to provide bibliometrics data for Alzheimer disease research.Methods Using bibliometrics methods,the characteristics and contents of Alzheimer disease research documents were analyzed in Pubmed database and Wanfang web by analysis tools.Research status of Alzheimer disease including the type of literatures and journal distribution from 2008 to 2012 was analyzed.Currently hot topics or top high frequency words were emphatically analyzed.Results There were 646 matched literatures for Alzheimer disease retrieved in Chinese core periodicals,and 19 423 literatures in Pubmed database.The articles for clinical study of Alzheimer disease were more than experimental and epidemiologic study among the 646 literatures.The disciplines distribution of AD in Chinese core journals was similar to international journals,mainly including nerves,brain,and geriatrics journals.The domestic research papers on Alzheimer disease were less than 5％ in Pubmed database,and the articles from Chinese journals in Chinese or English edition accounted for only a small proportion of Pubmed database.The recent research direction of Alzheimer disease in China was similar to foreign countries such as amyloid proteins,diagnosis and gene,but foreign research paid more attention to the metabolism,image and the aging mechanism in AD,while in China there were quite a lot of reports in the literatures on cholinergic drugs,tau protein,hippocampus and Chinese herbal treatments.Conclusions The AD-related disciplines distribution in China and foreign country is relatively fixed in recent 5 years.The AD researches carried out in China are close to those in foreign country,but researches on some areas still lag behind.The experimental and epidemiologic study on AD should be strengthen in China.

Objective To analyze the structural,content and language characteristics of English abstract in domestic versus international geriatrics journals in order to promote international exchange in geriatric medicine research in our country.Methods In this paper,we analyzed the characteristics and contents of original articles published in 5 foreign journals (IF＞3.0) from Pubmed database (n=60) and 5 core (the source of statistics) domestic geriatric journals from Wanfang web during recent 3 years (n=61).Results Our survey showed that in the domestic geriatrics journals,the structured abstracts were used with inflexible four-heading and fixed leading words.In foreign geriatrics journals,however,non-structured abstracts were mostly applied in articles on basic research,and structured abstracts primarily appeared in clinical or survey research papers.Compared with foreign geriatrics journals,the shortages or some problems in English abstracts of the domestic geriatrics journals included a short writing,repetition of objective with title,lack of description of research background,improper description of results data,more passive voice,improper use of modal verbs.Conclusions The domestic geriatrics journals should learn the diverse forms of abstracts and content structure from foreign geriatrics journals,strengthen the elaboration of background and highlights,and use active voice in concordance with the international mainstream.