Humans ; Infant, Newborn ; Meconium ; Peritonitis ; etiology

Child ; Humans ; Hypertension, Pulmonary ; complications ; Male ; Pulmonary Heart Disease ; complications ; Sleep Apnea, Obstructive ; complications

Objective: To further study the chemical constituents of Gymnotheca chinensis. Methods: The compounds were isolated and purified by silica gel column chromatography and preparative HPLC, and their structures were elucidated by spectral analysis. Results: Sixteen compounds were isolated and identified as 2-(1-hydroxy-4-oxo-cyclohexa-2,5-dienyl)-acetonitrile (1), N-benzoyl-2-hydroxyltyramine (2), N-benzoyltyramine (3), N-benzoyl-2-phenylethylamine (4), 7,4'-dimethoxykaemferol (5), nobiletin (6), tangeretin (7), curculonone A (8), 6-hydroxy-4,7-megastigmadien-3,9-dione (9), 5,6-epoxy-3-hydroxy-7-megastigmen-9-one (10), 9-hydroxy-4-megastigmen-3-one (11), 9-hydroxy-4,7-megastigmadien-3-one (12), loliolide (13), 6β-hydroxy-stigmasta-4-en-3-one (14), 6β-hydroxy- stigmasta-4,22-dien-3-one (15), and 3β-hydroxy-stigmasta-5-ene-7-one (16). Conclusion: Compounds 1 and 2 are new natural products, and the others are isolated from this genus for the first time.

Objective: To further study the chemical constituents of Clematoclethra scandens subsp. actinidioides. Methods: The compounds were isolated and purified by chromatographic methods such as silica gel column chromatography and preparative HPLC, and their structures were identified on the basis of physicochemical properties and spectral data. Results: Nineteen compounds were isolated and identified as vulgarsaponin A (1), 9-hydroxy-megastigma-4,7-dien-3-one-9-O-β-D-glucopyranoside (2), kaempferol-3-O- α-L-rhamnpyranoside (3), quercetin-3-O-α-L-rhamnopyranoside (4), quercetin-3-O-β-D-glucopyranoside (5), hyperoside (6), 1,3,6-tri-O-galloyl-β-D-glucopyranoside (7), 1,2,3,6-tetra-O-galloyl-β-D-glucopyranoside (8), catechin (9), gallocatechin (10), procyanidin B3 (11), protocatechuic acid (12), syringic acid (13), vanillic acid (14), 7-oxokaurenolide (15), stigmast-3,6-dione (16), stigmast-4-en-3,6-dione (17), taraxerol (18), and β-sitosterol (19). Conclusion: Compounds 1-12, and 16 are isolated from the plant of Clematoclethra Maxim. for the first time.

The factor VIII gene is located on the X chromosome,making haemophilia A, a sex-linked disorder. Thus on pedigree grounds all daughters of such patients are obligate carriers. One female case of haemophilia A was reported and related literatures were reviewed.
Child ; Chromosomes, Human, X ; Epistaxis ; etiology ; genetics ; Factor VIII ; Female ; Hemophilia A ; complications ; genetics ; Humans

Objective: To investigate the chemical constituents of Dendrobium nobile from Guizhou province. Methods: The constituents were isolated and purified by silica gel, MCI column chromatography, and preparative HPLC technology. The structure of the isolated compound was elucidated by MS and NMR spectra. Results: A new sesquiterpene, identified as δ-cadinen-12,14-diol (1), was isolated from the stem of D. nobile. Conclusion: Compound 1 is a new compound.

OBJECTIVETo observe therapeutic effects of locking titanium plate for the treatment of comminuted proximal humeral fracture in elderly.

METHODSFrom June 2011 to May 2013, 72 elderly patients with comminuted proximal humeral- fractures were divided into locking titanium plate group and anatomical plate group, 36 cases in each group. In locking titanium plate group, there were 16 males and 20 females aged from 60 to 79 years old with an average of (69.55±5.62) years old; 10 cases were type Neer II, 18 were type Neer III and 8 cases were type Neer IV in accordance with Neer classification; treated with locking titanium plate. In anatomical plate group, there were 15 males and 21 females aged from 60 to 81 years old with an average of (69.76±5.70) years old; 9 cases were type Neer II, 20 were type Neer III and 7 cases were type Neer IV; and treated with anatomical plate. Clinical effects, preoperative and postoperative Neer scoring, operative time, bone healing time and incidence of complications between two groups were compared.

RESULTSAll patients were followed up from 1 to 3 years with an average of 15 months. The excellent and good rate of locking titanium plate group (91.7%) was significantly higher than anatomical plate group (75.0%). Postoperative Neer score of two groups were improved obviously, but locking titanium plate group (92.51±7.85) was higher than anatomical plate group (83.64±8.56); there was no significant differences between two groups in operative time (P>0.05); bone healing time in locking titanium plate was (18.6±3.4) weeks, and shorter than anatomical plate group (24.3±3.9) weeks; incidence of complications in locking titanium plate was (5.6%) shorter than anatomical plate group (22.2%), and had obviously differences between two groups.

CONCLUSIONLocking titanium plate for the treatment of comminuted proximal humeral fracture in elderly plays an important role in good rate, bone healing time and Neer score. It has advantages of early rehabilitation exercise, less shoulder pain, rapid recovery of shoulder joint, less complications, safe and effective, and be worthy of clinical application, especially for senile osteoporosis patients.

Aged ; Aged, 80 and over ; Bone Plates ; Case-Control Studies ; Female ; Fractures, Comminuted ; surgery ; Humans ; Male ; Middle Aged ; Shoulder Fractures ; surgery ; Titanium

Adenocarcinoma, Clear Cell ; Female ; Humans ; Middle Aged ; Nasal Cavity ; Nose Neoplasms

Dietary Proteins ; administration & dosage ; Energy Intake ; Female ; Hospitalization ; Humans ; Infant, Newborn ; Infant, Very Low Birth Weight ; Logistic Models ; Male ; Parenteral Nutrition ; Weight Gain

Objective:To evaluate the impact of sample pooling strategy on 2019-nCoV RNA detection results.Methods:Ten negative swabs were stored in 6 ml virus transport medium, mixed thoroughly and diluted 1∶2 and 1∶10. Inactivated 2019-nCoV culture medium was added to simulate pooling samples: 10 pooling samples, 5 pooling samples and 1 swab sample. Extraction and amplification were made using three nucleic acid extraction reagents a, b, and c with different extraction methods and systems, as well as five 2019-nCoV detection reagents A-E with various template loading volumes and sensitivities respectively.Results:For the same sample, the Ct values of extracted templates a were 2.10±0.47 and 3.46±0.62 earlier than extracted templates b and c. For samples with identical amplifying, the Ct valves of N and ORF1ab gene of A reagent were 1.16±0.48 and 2.36±0.54 earlier than that of reagent B. Adding nucleic acid of 10 negative swabs to the amplification system lagged the Ct values of reagent A by about 1.36±0.32 Ct, while Ct values of reagent B were not affected. Extracted by regent a, a lag of 1.66±0.39 Ct on average was observed in C, D, and E reagents in detecting pooling samples of ten swabs as compared with one swab sample. When extracting 400 copies/ml pooling samples of ten swabs by reagent a, N gene could be detected by reagents C and E, but not by reagent D.Conclusion:Large amount of extraneous DNA is introduced by sample pooling, which could interfere the effiency of extraction and amplification. Strategies of using extraction reagents with large loading volume and high effiency, together with amplification reagents with large template volume and low limit of detection are helpful for ensuring detection sensitivity of pooling samples, and greatly reducing the risk of false negative results.