1.Analysis of poor distance visual acuity after refractive rotationally asymmetric multifocal intraocular lens implantation in cataract patients
Dongmei HUO ; Wei CHEN ; Danjie NIE ; Shuai SHI
Journal of Chinese Physician 2021;23(5):658-662
Objective:To analyze the cause of poor distance visual acuity after refractive rotationally asymmetric multifocal intraocular lens (IOL) implantation in cataract patients.Methods:The data were collected from patients who underwent cataract phacoemulsification combined with intraocular lens (refractive rotationally asymmetric multifocal intraocular lens, SBL-3) implantation at Beijing Aier Intech Eye Hospital from December 2016 to December 2018, and followed up for six months after operation. Standard logarithmic visual acuity chart was used to record uncorrected distance visual acuity, best corrected distance visual acuity, intermediate visual acuity, and near visual acuity. Defocus curve was drawn. The corneal topography, postoperative visual quality and intraocular lens centered or not were checked by Itrace.Results:Total 95 eyes of 70 patients were implemented by cataract phacoemulsification combined with regional refractive intraocular lens operation. Among them, uncorrected distance visual acuity of 16 eyes of 16 patients, were less than or equal to 0.6. The distance power zone of the intraocular lens of 7 patients were placed in the area of high corneal refractive power. Because of measurement error, reserved error, etc, the power of IOL in 7 patients had deviations. Farsightedness and dizziness occurred in 2 patients. The visual discomfort symptoms disappeared and vision improved through intraocular lens positioning.Conclusions:The poor quality of distance visual acuity is mainly caused by the coincidence of the distance power zone with the corneal high-refractive region, the deviation of the power of the intraocular lens, and the intraocular lens center shift. If the cornea has a high refractive area, the distance power zone should avoid to be placed in the same area. The intraocular lens should be reserved between 0-+ 0.25 D. Two-step surgery is recommended. It′s also recommended to make sure the lens is centered during the operation by lens reflection method.
2.Molecular cloning, sequence characterization and mRNA tissue expression analysis of TDRP1 gene from the Banna minipig inbred line (BMI)
Pei WANG ; Jinlong HUO ; Shuyan WANG ; Weirong PAN ; Xingqin ZHA ; Chen SHI ; Yangzhi ZENG
Acta Laboratorium Animalis Scientia Sinica 2014;(6):9-16
Objective To get TDRP1 gene of sterile and fertile boar of the Banna minipig inbred line (BMI), predict its function by bioinformatics analysis, and detect its expression patterns in the fertile boar.Methods Based on the NM_001198925 sequence, we designed specific primers and amplified BMI TDRP1 using RT-PCR method for sequen-cing and bioinformatics analysis.Meanwhile, the expression of TDRP1 in 17 organ tissues ( heart, liver, spleen, lung, kidney, thymus, lymph nodes, skin, duodenum, stomach, cerebrum, cerebellum, testis, epididymis, seminal vesicle, prostate, and bulbourethral gland) of fertile BMI boar and in the testis of sterile and fertile BMI boars was analyzed by semi-quantitative RT-PCR.Results The experiment obtained 680 bp cDNA sequence ( GenBank accession number:KJ186786) of BMI TDRP1, which encodes a protein of 186 amino acids with a predicted molecular weight (Mw) of 20.49 kDa and isoelectric point (pI) 5.86, and no signal peptide.It was a nuclear protein with a probability of 94.1%and had a leucine-rich nuclear export signals.Homology analysis of protein sequences revealed that BMI TDRP1 showed high identi-ty with that of humans, macaca mulatta, mouse and rat.The RT-PCR analysis showed that TDRP1 had a similar expression in the testes of sterile and fertile BMI boars.It was highly abundant in the seminal vesicle and prostate, moderately ex-pressed in cerebellum and testis and weakly expressed in cerebrum and kidney, while undetected in other 11 organ tissues. Conclusions We have cloned TDRP1 complete coding sequence, and found 2 SNPs,showing no difference in sequences and the testis mRNA expression levels between the fertile and sterile BMI boars.The multi-tissue transcription profile shows different expression levels in different organ tissues, being high in the seminal vesicle and prostate.The results of this study provide a foundation for further insight into the role of this gene in spermatogenesis.
3.Establishment and evaluation of a multi-system chain transitional care programme for postpartum pelvic floor training
Ran HUO ; Huizhen WANG ; Lanping SHI ; Weiying ZHANG ; Zhenyun WU ; Xueli CHEN
Chinese Journal of Practical Nursing 2014;30(28):29-32
Objective To build a multi-system chain transitional care programme for postpartum pelvic floor training and test its effect.Methods By a prospective randomized controlled trial,the study group (n=60) received a multi-system chain transitional care programme which consisted of ward nurse,home visiting doctor,post-natal care clinic doctor,pelvic floor doctor and pelvic floor nurse.The control group(n=60) received routine care.The pelvic floor muscle strength,sexual function and patient satisfaction of baseline,on the 42th days,3rd months and 6th months were compared between two groups by short-term follow-up.Results There was no difference in baseline consisting of age,gestational weeks,birth weight and so on between groups.Participants in the study group had significantly better improvement and score in pelvic floor muscle strength,sexual thoughts,sexual arousal,sexual psychopathy and problems affecting sexual function.There was no difference in score of sexual pleasure and ejaculation time between two groups.Conclusions This study was an original effort to establish and evaluate a multi-system chain transitional care program for postpartum pelvic floor training.Results demonstrated that this transitional care was effective in improving pelvic floor function.
4.Effect of Methylprednisolone on expression of aquaporin 1 in lung tissue of pulmonary edema rats induced by adrenaline
Keling WANG ; Longge WANG ; Ling CHEN ; Rongfu SHI ; Xiaoming WANG ; Ximin HUO
Chinese Journal of Applied Clinical Pediatrics 2017;32(9):682-686
Objective To observe the changes in the lung aquaporin 1 (AQP1) expression in adrenaline-induced pulmonary edema(PE),and the effect of Methylprednisolone (MP) on its expression.Methods Fifty Wister rats of 1-month old were randomly divided into 5 groups (10 rats in each group):control,adrenaline PE,MP A,MP B,and MP C groups,respectively.Control group animals were treated with 0.27 mL 9 g/L saline;PE group was given 2.7 mg/kg adrenaline (1 ∶ 1 000) by intraperitoneal injection;MP A,MP B and MP C groups rats were intraperitoneally injected 10 mg/kg,20 mg/kg,and 30 mg/kg MP intraperitoneally immediately after intraperitoneal injection of adrenaline,respectively.The morphology changes in the lungs were observed with HE staining,and lung wet/dry weight (W/D) was measured.The levels of AQP1 mRNA,AQP1 protein,and AQP1 distribution in the lung tissues were detected by using real time-polymerase chain reaction,Western blot,and immunohistochemical method.Results (1)PE group exhibited a faster breathing rate,and double lung volume increased significantly;there was a visible hemorrhagic distribution in the lung surface and cross section,endotracheal filled with white or pink foam liquid.(2) The W/D of rats in PE group was higher than that of the control group (6.50 ± 0.53 vs.4.59 ± 0.36,P < 0.05).(3) Pathological grading of PE group (3.80 ± 0.42) increased significantly compared with that of the MP A group (3.30 ± 0.48),MP B group (2.30 ± 0.68) and MP C group (1.20 ± 0.42),and there were significant differences (all P < 0.05).(4) Immunohistochemistry showed that the expression of AQP1 in PE group (1.20 ± 0.79) was reduced compared with that of the control group (4.20 ± 1.03),and there were significant differences (all P < 0.05).(5) The levels of AQP1 mRNA and AQP1 protein (0.12 ± 0.43 and 0.20 ± 0.04) were significantly lower than those of the control group (0.90 ± 0.32 and 0.60 ± 0.15),and there were significant differences (all P < 0.05);compared with PE group,AQP1 mRNA and AQP1 protein of each group with MP treatment showed the highest values (MP A group:0.17 ±0.06 and 0.32 ±0.04,MP B group:0.39 ±0.13 and 0.37-±0.09,MP C group:0.61 ±0.21 and 0.44 ± 0.07) (all P < 0.05).Conclusion The expression of AQP1 reduced in adrenaline-induced PE rats.MP could improve the expression of AQP1,and significantly ameliorate the PE and bleeding.
5.A survey of chikungunya virus in brain tissue samples from rat like animals in Xiamen, Shenzhen and Guangzhou
Shuting HUO ; Yun MO ; Yongxia SHI ; Yiquan XIONG ; Dongliang LI ; Min QIU ; Xueyan ZHENG ; Shaowei CHEN ; Lina JIANG ; Qing CHEN
The Journal of Practical Medicine 2017;33(5):820-822
Objective To examine the prevalence of chikungunya virus in brain tissue samples from rat?like animals in Xiamen, Shenzhen and Guangzhou, and to explore whether the rat?like animals are potential sources of human chikungunya fever infections and the host of the virus. Methods Rat?like animals were trapped in residential areas, city parks, hospitals, markets and schools in Xiamen, Shenzhen and Guangzhou (Yuexiu and Baiyun districts) between January 2013 and June 2016. Brain tissue samples of the trapped animals were collected under sterile. Chikungunya virus was detected by using reverse transcription polymerase chain reaction (RT?PCR). Results Totally 1092 rat?like animals were trapped, which belonged to 7 species, 3 genera, 2 families, 2 orders. Rattus norvegicus was the dominant species in the indoor environment, Rattus losea was dominant in wild environment, and 1092 brain tissue samples were collected. No detectable chikungunya virus was found in the brain tissue samples by RT?PCR. Conclusion There is a low possibility that rat?like animals act infectious sources of human chikungunya fever infections and the host of the virus.
6.Use of melatonin in rats with acute paraquat posoning
Xianli GUO ; Fei TONG ; Yingping TIAN ; Hanwen SHI ; Shuhua HUO ; Lin HU ; Hui CHEN ; Lidou LIU ; Hembo CAO ; Xia WANG
Chinese Journal of Emergency Medicine 2008;17(9):948-951
Objective To investigate the effects of melatonin (MT) on rats with acute paraquat (PQ) poisoning. Method Fifty-four Sprague-Dawley (SD) rats were randomly divided into three groups (each group 18 rats) and given the following treatment: intragastric injection of PQ at 50 mg/kg (PQ); intragastric injection of paraquat followed by intraperitoneal injection of MT at 10mg/kg once a day (MT); intragastric injection of normal saline (Control). Serum assays for malondialdehyde (MDA) levels and superoxide dismutase (SOD) and glu tathione peroxidase (GSH-Px) activities were determined on the 1st, 3rd and 7th day post treatment. Clinical manifestations of poisoning and pathological changes in the lungs were also observed. Results Serum MDA levels were significantly increased (P < 0.01), and SOD and GSH-Px activities significantly decreased (P < 0.05) in the PQ group compared to the control group. Serum MDA levels were significantly decreased, and serum SOD and GSH-Px activities increased in MT group compared to the PQ group (P < 0.05). Clinical manifestations of intoxication and pathological lung changes were also ameliorated in poisoned rats treated with MT. Condutions Administration of MT alleviates clinical manifestations of acute paraquat poisoning in rats by Limiting the damage from lipid peroxidation.
7.Way of emergency treatment on patients with traumatic brain injuries in Guangdong mountain area
Jin-Zhao ZHANG ; Yong-Gao DENG ; Shi-Huo CHEN ; Zhi-Sheng CHEN ; Jing LI ; Zhi-Piao XIE
Chinese Journal of Neuromedicine 2010;9(5):500-502
Objective To investigate the new way that can improve the emergency treatment rate on patients with traumatic brain injuries in Guangdong mountain area. Methods New emergency treatment way was adopted from June 2006 to June 2008 in patients traumatic brain injuries and these patients were chosen as experimental group. Control group is composed of patients treated with old emergency treatment way from June 2004 to June 2006. The pre-hospital mortality rate, in-hospital mortality rate and CPR success rate in the 2 groups were compared; the interphase between injury and receiving treatment, and the rescuing radius in these 2 groups were analyzed. Results Control grouphad the in-hospital mortality rate and pre-hospital mortality rate reaching 29.06% (68/234) and 32.76%(114/348), respectively, which was significantly higher than the experimental group (in-hospital mortality rate: 10.49% [28/267], pre-hospital mortality rate:18.10% [59/326]) (P<0.05). The maxima rescuing radius and mean interphase between injury and receiving treatment in the experimental group (10 km, 50min) were statistically different as compared with those in the control group (70 km, 85 min) (P<0.05).CPR success rate in the experimental group (80.32%) was obviously higher man that in the control group (23.24%) (P<0.05). The death rate in the experimental group was increased following the prolonged interphase between injury and receiving treatment. Conclusion The new way, by increasing 120urgent care centre, shortening the rescuing radius and reaction time, strengthening the care technique ofthe doctors and nurses and reinforcing the equipment in the urgent care centre, can obviously increase the emergency treatment rate.
8. Optimization of DXA lumber spine bone imaging parameters based on Monte Carlo method and CT voxel phantom
Shi LI ; Yifei PI ; Wanli HUO ; Zhi WANG ; Zhi CHEN ; Xie XU
Chinese Journal of Radiological Medicine and Protection 2018;38(7):541-546
Objective:
To study the lumber spine imaging process of dual-energy X-ray absorptiometry (DXA) and parameters used to optimize the image quality.
Methods:
A computational voxel phantom was constructed from patient computed tomography (CT) data. Using the Monte Carlo radiation transport method, a dual energy x-ray beam was simulated to scan the phantom of lumbar spine to generate a bone density image. The Figure of Merit (FOM) of each image was claculated. Parameters including the combination of the high and low energy tube voltage, the thickness of Cu filter, and the ratio of two beam energy incident photon number were optimized, which based on FOM.
Results:
FOM reaches a minimum of 1.59 × 10-2 with the tube voltage combination of 75 and 200 kVp. With the thickness of the Cu filter from 0 mm to 3 mm, FOM decreases from 6.30×10-2 to 1.87×10-2, showing a gradually slow-down trend. With the incident photon number ratio (low energy/high energy) increasing from 1∶3 to 19∶1, FOM decreases firstly and then increases, reaching a minimum of 1.40×10-2 at 3∶1.
Conclusions
According to the simulation results, the combinations of low tube voltage from 70 kVp to 85 kVp and high tube voltage from 160 kVp to 200 kVp, 0.3 mm Cu filter and beam incident photon number ratio from 1 to 5 can yield the best lumbar spine image quality with the lowest patient dose.
10.Comfort of crew and passengers and atmospheric pressure, noise, wind speed in high-speed train of Shijiazhuang-Taiyuan passenger dedicated line.
Yi-biao ZHAI ; Wei HUO ; Qiao-ying LIU ; Bao-shan CHEN ; Jin-long ZHANG ; Lei SHI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(11):849-851
OBJECTIVETo explore the crew and passengers' comfort on the Shijiazhuang-Taiyuan passenger dedicated line and physical factors, such as air pressure, noise, wind speed.
METHODSComfort investigation of all the crew (n = 244) and passengers (n = 377) on the Shijiazhuang-Taiyuan passenger dedicated line at speed of 250 km/h and 200 km/h and the detection of the air pressure, noise and wind speed were performed in 2011.
RESULTSSignificantly higher ratio of comfortable feeling, lower ratio of seriously discomfortable feeling were observed in crew and passengers at 200 km/h compared with those at 250 km/h (P < 0.05), as well as rapid disappearance of discomfortable feeling in crew (P < 0.05) and significantly higher ratio of lightly discomfortable feeling and lower ratios of tinnitus and eardrum discomfort induced by air pressure and noise in passengers at 200 km/h. No significant difference was observed in ear discomfort induced by air pressure and noise among crew, and the duration of disappearance of discomfortable feeling among passengers between 200 km/h and 250 km/h. The noise in carriages exceeded the related standard when the high-speed train passing through the tunnels.
CONCLUSIONThe individuals feel more comfortable at 200 km/h than 250 km/h in this line., which may be related with rapid variation of wind speed and noise when the train passes through the tunnels with high speed.
Adolescent ; Adult ; Atmospheric Pressure ; Female ; Humans ; Male ; Noise, Occupational ; Noise, Transportation ; Occupational Health ; Railroads ; Sensation ; Surveys and Questionnaires ; Wind ; Workplace ; Young Adult