1.Reversal of multidrug resistance by neferine in human gastric carcinoma cell line SGC7901/VCR
Shuhong SHI ; Hui ZHANG ; Yingzhi ZHUANG
China Oncology 2001;0(02):-
Purpose:To study the reversal of multidrug resistance (MDR) and its mechanism by neferine which is a new calcium channel blocker in human gastric carcinoma cell line SGC7901/VCR. Methods:The cytotixic effect was tested by MTT assay. The expression of multidrug-resistance-associated protein in human gastric carcinoma cells was examined by SP immunocytochemical and flow cytometry. Results:Neferine at the concentration 10 ?mol/L was not of significant cytotoxicity to SGC7901 and SGC7901/VCR cells. Neferine at the concentration of 2.5,5,10 ?mol/L decreased the IC_(50)value of VCR to SGC7901/VCR cells from 2.32 ?g/ml to 0.340,0.128 and 0.053 ?g/ml, respectively and with the increase by 6.8-,18.1-and 43.8-fold in the chemosensitivity, respectively. It had more potent reversal action on SGC7901/VCR cells than Verapamil at the concentration of 10 ?mol/L(P
2.DETECTION AND LOCATION OF MELATONIN RECEPTOR IN HUMAN SKIN AND ITS MOLECULAR FEATURE
Yongquan SHI ; Hui ZHANG ; Zhimin LIU
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
In order to detect and localize melatonin receptor and its mRNA expression in the human skin, human skin was obtained from healthy volunteers. Immunohistochemistry and RT-PCR were applied to detect MR in human skin and to identify its subcellular distribution. Electrophoresis of RT-PCR product showed positive band of mt 1 subtype in human skin. The subtype of MR, mt 1was present in the membrane, cytosol and nucleus of skin cells as shown by immunohistochemistry. It is suggested that skin is the target organ of melatonin.
3.The application of breast specific gamma imaging and positron emission mammography in the diagnosis and therapy of breast cancer
Hui TAN ; Yiqiu ZHANG ; Hongcheng SHI
Chinese Journal of Nuclear Medicine and Molecular Imaging 2014;34(1):73-75
Breast-specific gamma imaging (BSGI) and positron emission mammography (PEM) have the high resolution in diagnosing breast lesions with minimum diameter of 3 mm.Both BSGI and PEM are functional imaging modalities,which have no relation with breast tissue density,implanted prosthesis,scar formation and so on.This review elaborates the application of BSGI and PEM in the early diagnosis,treatment protocols and evaluation of efficacy for the patients with breast cancer.
4.Changes and its clinical significance of serum brain-derived neurotrophic factor level and plasma tissue plasminogen activator level in patients with amnestic mild cognitive impairment
Hui YU ; Zhijun ZHANG ; Yongmei SHI
Journal of Clinical Neurology 1993;0(03):-
Objective To investigate the changes and its clinical significance of serum brain-derived neurotrophic factor (BDNF) level and plasma tissue plasminogen activator( t-PA ) level in patients with amnestic mild cognitive impairment (aMCI). Methods 99 aMCI patients (aMCI group) and 99 matched normal controls (NC group) were recruited for the present study. Multi-dimension neuropsychologic tests were used to extensively assess cognitive function of subjects. An enzyme-linked immunosorbent assay was applied to measure serum BDNF level and plasma t-PA level. The correlation analysis between serum BDNF level and plasma t-PA level tested by ELISA and cognitive function were also made. Results (1) The results neuropsychologic tests in aMCI guoup were significantly lower than those in NC group (all P
5.Clinical observation of ruangan suopi tablet in treating chronic hepatitis B caused liver cirrhosis.
Jia-fu LI ; Hui-qin ZHANG ; Peng-hui SHI
Chinese Journal of Integrated Traditional and Western Medicine 2002;22(3):188-189
Adult
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Hepatitis B, Chronic
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complications
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drug therapy
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Humans
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Liver Cirrhosis
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drug therapy
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etiology
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Male
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Middle Aged
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Phytotherapy
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Tablets
6.Effects of Omeprazole Combined with Somatostatin on Intra-Abdominal Pressure and Intestinal Mucosal Barrier Function of Elderly Patients with Severe Acute Pancreatitis
Hui ZHANG ; Niu SHI ; Miao ZHANG ; Yuting YUN ; Jie SONG
Progress in Modern Biomedicine 2017;17(24):4672-4675
Objective:To explore the effects of omeprazole combined with somatostatin on intra-abdominal pressure and intestinal mucosal barrier function of elderly patients with severe acute pancreatitis.Methods:Selected 82 cases of elderly patients with severe acute pancreatitis from our hospital.They were divided into research group (48 cases) and control group (34 cases).The control group was treated with basic therapy,and the research group was treated with omeprazole combined with somatostatin on the basis of the control group.Analyzed and compared the clinical effects,intestinal mucosal barrier function and symptom indexes of patients in the two groups.Results:①The total effective rate of the research group was significantly better than that of the control group,and the difference was statistically significant (P=0.005).②After treatment,the D-lactose and diamine oxidase (DAO) levels of both groups were significantly lower than before treatment,and the difference was statistically significant (both P<0.001).In addition,after treatment,the D-lactose and DAO levels were significantly lower in the research group than in the control group,and the differences were statistically significant (mean P < 0.001).③After treatment,patients of the research group showed better results in abdominal pain and abdominal distension relief time,bowel recovery time,hospital stay and blood amylase index than those in the control group,and the difference was statistically significant (mean P < 0.001).④After treatment,the intra-abdominal hypertension of two groups was significantly lower than before treatment,with statistical difference (P < 0.001).Yet it was significantly lower in the research group than in the control group,with statistical difference (P < 0.001).Conclusion:The combined treatment of omeprazole and somatostatin can effectively improve the clinical efficacy of severe acute pancreatitis in elderly patients,correct intra-abdominal hypertension and improve the intestinal mucosal barrier function of patients,so it is worthy of reference and promotion.
7.Imaging Diagnosis of Cystitis Glandularis: A Report of 8 Cases
Xiangming SHI ; Hui WANG ; Qiang ZHANG ; Xiuping LI ; Bo QU
Journal of Practical Radiology 2010;26(2):216-218
Objective To study of the imaging diagnosis and differential diagnosis of cystitis glandularis. Methods The clinical data and imaging findings of 8 cases with cystitis glandularis proved by pathology were retrospectively analyzed with literaures re-view. Results The lesions on images appeared as nodular mass in 4 cases, diffuse in 2 cases and mixed type in 2 cases,1 case with cystic degeneration. The lesions in 2 cases obviously reduced in size after treatment. The CT value of the lesions post contrast-en-hanced scan averagely increased up to 15.8 HU compared with that of plain scan. The lesions were localized in 6 cases and diffuse in 2 cases. Conclusion cystitis galandularis has some imaging characteristics, but the final diagnosis is depended on pathology.
8.Compound ocimum oil oral suspension in the treatment of 382 children with acute diarrhea (Piweixure Syndrome)
Zhenyu WANG ; Yu CHEN ; Lei ZHANG ; Hui SHI ; Yan LI
Chinese Journal of Primary Medicine and Pharmacy 2015;22(1):23-25
Objective To investigate the efficacy and safety of compound ocimum oil oral suspension in the treatment of children with acute diarrhea(Piweixure Syndrome).Methods 382 children with acute diarrhea(Piweixure Syndrome) were selected for the multi center and open clinical trial.All patients was treated by compound ocimum oil oral suspension based on age and weight,6 months below 3mL thrice a day,6-< 12months 5mL thrice a day,12-<24 months 10mL thrice a day,24 months upper 20-40 mL thrice a day.The course was three days.The efficacy and safety was observed.Results The total efficiency was 93.98%,there were 274 cases with marked effect and 85 case with effective in analysis on clinical efficacy.There were significant differences between before and after therapy on symptoms and signs as defecation frequency,defecation characters and defecation disesthesia (x2 =467.43,524.51,263.03,all P < 0.05).Conclusion Compound ocimum oil oral ouspension in the treatment of children with acute diarrhea(Piweixure Syndrome) has definite effect and safety.It would be applied to clinical wildly with advantages as shorten the course,decreased diarrhea times and improved clinical symptoms.
9.The evaluation of insulin resistance and ?-cell function in patients with systemic lupus erythematosus
Hui GUO ; Yali ZHANG ; Shumei WANG ; Bingyin SHI
Journal of Xi'an Jiaotong University(Medical Sciences) 2003;0(06):-
Objective To evaluate insulin resistance and ?-cell function in patients with systemic lupus erythematosus(SLE).Methods The study group included thirty-two female SLE patients who were hospitalized or received outpatient treatment in our hospital;the control group consisted of twenty-two healthy females.Their fasting plasm glucose and serum insulin were all tested.Homeostasis model assessment(HOMA) was used to calculate HOMA-IR and HOMA-?-cell function,and these parameters were compared between the study group and control group.Results Compared with control group,the SLE patients had significantly higher fasting insulin levels(12.13?4.32 vs.8.84?2.19,P0.05).Conclusion Patients with SLE have a higher risk of insulin resistance and abnormal insulin secretion than controls.
10.The interaction between nitric oxide and hydrogen sulfide on the relaxation reactivity of pulmonary arteries in rats
Lin SHI ; Junbao DU ; Chunyu ZHANG ; Hui YAN ; Chaoshu TANG
Chinese Pharmacological Bulletin 2003;0(10):-
Aim To explore the interaction between nitric oxide( NO) and hydrogen sulfide(H 2S) on the relaxation reactivity of pulmonary arteries in rats.Methods Seven male healthy rats were anaesthesed with chloral hydrate; the pulmonary artery of each rat was removed for the study. Th e reactivities of pulmonary artery rings in response to different doses of NO do nar-sodium nitroprusside(SNP) and H 2S donar-NaHS were measured in vitro.DL-propargylglycine(PPG)and N?-nitor-L-methyl ester(L-NAME) were provided to pulmonary artery, respectively;the relaxation reactivities of pulmonary artery were observed.Results The relaxation reactivities of pulmonary arteries showed a dose-dependent increase in response to different doses of SNP and H 2S.The relaxation reactivity to SNP decreased by PPG. The relaxation reactivity to H 2S decreased by L-NAME.Conclusion H 2S acted as a vasorelaxator either independently or accompanied with NO, SNP acted as a vasorelaxator either independently or accompanied with H 2S;the networ k of gastransmitter played an important role in the relaxation of pulmonary arte ries.