6.Influence of the fetal microchemirism in the thyroid glands on the Graves' disease animal model
Dan PU ; Hui GUO ; Aiqun MA ; Binying SHI
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
Objective To explore the influence of the fetal microchemirism in the thyroid tissue on the Graves' disease(GD) animal model.Methods Mice in experimental group were injected with Ad-TSH289(5?108IU) to construct the GD animal model,and when the immunization was over,the experimental group was randomly divided into three parts again: two groups were mated with male mice until two weeks of pregnancy or two weeks post-partum while the other group was simple immunized with Ad-TSH289 only.SRY was tested by real-time PCR.Thyroid tissue was stained with HE for pathological examination.Results Histological examination of the thyroid gland revealed that 61.8% of simple immunized mice had goiter,the success rate of GD animal model was 38.5% in pregnancy group,but 76.9% in postpartum groups(P
7.STUDIES 0N THE COLON TUMOR INHIBITION AND ITS MECHANISMS OF PEPTIDOGLYCAN DERIVED FROM LACTOBACILLUS
Xiyi MA ; Gongwei LE ; Yonghui SHI ; Hui DAI
Acta Nutrimenta Sinica 2004;0(06):-
Objective: To investigate the antitumor effect of lactobacillus peptidoglycan (PG) and explore its possible mechanisms. Methods: The inhibition for colon tumor growth, and the influence on the activities of CTL, NK, PM?, IL-1, IL-2, TNF-?, IFN-?and nitric oxide secretion were investigated in mice by cell culture, DNA agrose gel electrophoresis, respectively. Results: Growth in vivo of CT26 colon tumor was significantly inhibited by ip injection of PG. PG also induced significantly the activities of IL-1, TNF-?, IFN-?, CTL, NK, PM?and the production of nitric oxide, but had no effect on the activity of IL-2. Conclusion: The peptidoglycan derived from lactobacillus inhibited the colon tumor growth through activation of murine immunity. The antitumor effect of PG maybe partly if not all, induced, by the enhanced production of IFN-?, TNF-?and NO, through activation of NK cell and PM?. Key word: peptidoglycan; antitumor; immunity; cell activity; cytokine
8.Secretory carcinoma of breast in male: report of a case.
Yan XU ; Qun-Li SHI ; Xiao-Jun ZHOU ; Heng-Hui MA ; Hang-Bo ZHOU
Chinese Journal of Pathology 2009;38(10):707-708
Adenocarcinoma, Mucinous
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metabolism
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pathology
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Adult
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Breast Neoplasms, Male
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metabolism
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pathology
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surgery
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Cadherins
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metabolism
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Carcinoma
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metabolism
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pathology
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surgery
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Carcinoma, Signet Ring Cell
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metabolism
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pathology
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Diagnosis, Differential
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Follow-Up Studies
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Humans
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Keratin-5
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metabolism
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Lymph Node Excision
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Male
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Mastectomy
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methods
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Mucin-1
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metabolism
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S100 Proteins
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metabolism
9.Meningeal melanocytoma with nevus fuscoceruleus ophthalmomaxillaris: report of a case.
Chun WU ; Hai WANG ; Qun-li SHI ; Heng-hui MA ; Zhen-feng LU
Chinese Journal of Pathology 2011;40(3):194-195
Adult
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Diagnosis, Differential
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Humans
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MART-1 Antigen
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metabolism
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Magnetic Resonance Imaging
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Male
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Medulloblastoma
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metabolism
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pathology
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Melanocytes
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pathology
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Melanoma
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diagnosis
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metabolism
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pathology
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surgery
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Melanoma-Specific Antigens
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metabolism
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Meningeal Neoplasms
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diagnosis
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metabolism
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pathology
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surgery
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Neoplasms, Multiple Primary
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diagnosis
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metabolism
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pathology
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surgery
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Neurilemmoma
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metabolism
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pathology
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Nevus of Ota
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diagnosis
;
metabolism
;
pathology
;
surgery
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S100 Proteins
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metabolism
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Skin Neoplasms
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diagnosis
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
10.Identification of medicinal plant Dendrobium based on the chloroplast psbK-psbI intergenic spacer.
Hui YAO ; Pei YANG ; Hong ZHOU ; Shuang-jiao MA ; Jing-yuan SONG ; Shi-lin CHEN
Acta Pharmaceutica Sinica 2015;50(6):783-787
In this paper, the chloroplast psbK-psbI intergenic spacers of 18 species of Dendrobium and their adulterants were amplified and sequenced, and then the sequence characteristics were analyzed. The sequence lengths of chloroplast psbK-psbI regions of Dendrobium ranged from 474 to 513 bp and the GC contents were 25.4%-27.6%. The variable sites were 71 while the informative sites were 46. The inter-specific genetic distances calculated by Kimura 2-parameter (K2P) of Dendrobium were 0.006 1-0.058 1, with an average of 0.028 4. The K2P genetic distances between Dendrobium species and Bulbophyllum odoratissimum were 0.093 2-0.120 4. The NJ tree showed that the Dendrobium species can be easily differentiated from each other and 6 samples of the inspected Dendrobium species were identified successfully through sequencing the psbK-psbI intergenic spacer. Therefore, the chloroplast psbK-psbI intergenic spacer can be used as a candidate marker to identify Dendrobium species and its adulterants.
Chloroplasts
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DNA, Chloroplast
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genetics
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DNA, Plant
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genetics
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DNA, Ribosomal Spacer
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genetics
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Dendrobium
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classification
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genetics
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Plants, Medicinal
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classification
;
genetics