1.Identification of medicinal plant Dendrobium based on the chloroplast psbK-psbI intergenic spacer.
Hui YAO ; Pei YANG ; Hong ZHOU ; Shuang-jiao MA ; Jing-yuan SONG ; Shi-lin CHEN
Acta Pharmaceutica Sinica 2015;50(6):783-787
In this paper, the chloroplast psbK-psbI intergenic spacers of 18 species of Dendrobium and their adulterants were amplified and sequenced, and then the sequence characteristics were analyzed. The sequence lengths of chloroplast psbK-psbI regions of Dendrobium ranged from 474 to 513 bp and the GC contents were 25.4%-27.6%. The variable sites were 71 while the informative sites were 46. The inter-specific genetic distances calculated by Kimura 2-parameter (K2P) of Dendrobium were 0.006 1-0.058 1, with an average of 0.028 4. The K2P genetic distances between Dendrobium species and Bulbophyllum odoratissimum were 0.093 2-0.120 4. The NJ tree showed that the Dendrobium species can be easily differentiated from each other and 6 samples of the inspected Dendrobium species were identified successfully through sequencing the psbK-psbI intergenic spacer. Therefore, the chloroplast psbK-psbI intergenic spacer can be used as a candidate marker to identify Dendrobium species and its adulterants.
Chloroplasts
;
DNA, Chloroplast
;
genetics
;
DNA, Plant
;
genetics
;
DNA, Ribosomal Spacer
;
genetics
;
Dendrobium
;
classification
;
genetics
;
Plants, Medicinal
;
classification
;
genetics
2.Expression of BSAP/CD30 in classic Hodgkin lymphoma using double-staining technique.
Yan-Feng XI ; Wen-Qi BAI ; Jin-Fen WANG ; Quan-Hong WANG ; Shi-Lan JIAO
Chinese Journal of Pathology 2007;36(2):136-137
Adolescent
;
Adult
;
Aged
;
Biomarkers, Tumor
;
metabolism
;
Child
;
Female
;
Gene Expression Regulation, Neoplastic
;
Hodgkin Disease
;
genetics
;
metabolism
;
Humans
;
Ki-1 Antigen
;
metabolism
;
Male
;
Middle Aged
;
PAX5 Transcription Factor
;
metabolism
;
Staining and Labeling
;
methods
;
Young Adult
3.Application of CK34?E12,p63 and P504S in diagnosis of prostate cancer
Cai-Hong WANG ; Jin-Fen WANG ; Zhen-Guo MI ; Shi-Lan JIAO ;
Cancer Research and Clinic 2001;0(04):-
Objective To evaluate the value of CK34?E12,p63 and P504S immunostaining in diag- nosis of benign and malignant lesions of the prostate.Methods Expression of CK34?E12,p63 and P504S in 74 benign and malignant lesions of prostate,including 27 PC,6 HGPIN,10 LGPIN,3 AAH and 28 BPH were observed by immunohistochemical method.Results Expression of p63 and CK34?E12 were positive in AAH,LGPIN and BPH,all of PC were negative,the positive rate of HGPIN was 83.3%(5/6).There were sig- nificant differences in p63 and CK34?E12 expression between PC and AAH,HGPIN,LGPIN,BPH(P
4.The Screening and Study of Arctic Marine Psychrophilic Bacterial for Anti-tumor and Antibacterial Activity
Hong-Qiang SHANG ; Tian-Jiao ZHU ; Qian-Qun GU ; Wei-Ming ZHU ; Hua-Shi GUAN ;
Microbiology 1992;0(05):-
In this study, 101 strains of bacteria were isolated from arct ic water and sediment samples. The methanol extracts of the fermented broth prod uced by these strains were screened in vitro for anti-tumor activity on mou se tsFT210 cells using the method of flow cytometry, and screened for antibacter ial activity by the method of paper disk diffusion. The result showed that one strain exhibited anti-tumor activity and eight strains had antibacterial activ ity. The stability of the antibacterial components produced by strain AR084 an d its optimum medium were also studied. The research indicated that arctic bac teria had potential application in pharmaceutics.
5.Application of Cook MOB-15 system in guiding wire insertion during endoscopic retrograde cholangiopancreatography
Yun-Xing SHI ; Xiao-Hong ZENG ; Jiao LV ; Guo-Zhong ZHOU ; Chang-Yun LIU ;
Academic Journal of Second Military Medical University 2001;0(09):-
Objective:To evaluate the value of Cook MOB-15 system in guiding wire insertion during endoscopic retrograde cholangiopancreatography (ERCP). Methods: The clinical data of 51 patients who received Cook MOB-15 system-guided wire insertion during ERCP between Jan. 2005 to Dec. 2007 were retrospectively analyzed. Forty patients who received conventional ERCP catheter for malignant jaundice between Jan. 2002 and Dec. 2004 were taken as control. The successful insertion rates were compared between the 2 groups. Results: The successful insertion rate was 90.2% (46/51) in the Cook MOB-15 system group and 72.5% (29/40) in the conventional group; there was significant difference between the 2 groups (P
6.Influence of particle size and MePEG molecular weight on in vitro macrophage uptake and in vivo long circulating of stealth nanoparticles in rats.
Chao FANG ; Bin SHI ; Ming-huang HONG ; Yuan-ying PEI ; Hong-zhuan CHEN
Acta Pharmaceutica Sinica 2006;41(4):305-312
AIMTo investigate the influence of particle size and methoxypolyethyleneglycol (MePEG) molecular weight on the in vitro macrophage uptake and in vivo long circulating of recombinant human tumor necrosis factor-alpha (rHuTNF-alpha)-loaded stealth nanoparticles in rats.
METHODSThree sizes (approximately 80, 70 and 240 nm) of poly (methoxypolyethyleneglycol cyanoacrylate-co-n-hexadecyl cyanoacrylate) (PEG-PHDCA) nanoparticles loading rHuTNF-alpha were prepared at different MePEG molecular weights (Mr 2,000, 5,000, 10,000) using the double emulsion method. The in vitro macrophage uptake and in vivo long circulating properties in rats were examined and compared.
RESULTSThe uptake by macrophages decreased and the half-life of rHuTNF-alpha in rat increased with the increase of MePEG molecular weight or the decrease of particle size. The linear-ships between particle size and MePEG molecular weight and the in vitro macrophage uptake and in vivo long circulating properties were fairly good. Having the highest MePEG surface density (1.32 nm(-2)) , the shortest average distance between neighboring MePEG chain (0.87 nm) and the thicker fixed aqueous layer thickness (FALT, 5.16 nm), PEG5,000-PHDCA nanoparticles (80.0 nm) earned the strongest potency of decreasing uptake by macrophages and prolonging the half-life of rHuTNF-alpha in rat.
CONCLUSIONWithin the experimental limits, particle size and MePEG molecular weight had dramatic influence on in vitro macrophage uptake and in vivo long circulating properties of rHuTNF-alpha-loaded stealth nanoparticles.
Animals ; Cyanoacrylates ; chemistry ; Drug Carriers ; chemistry ; Drug Delivery Systems ; Macrophages ; physiology ; Male ; Mice ; Molecular Weight ; Nanoparticles ; Particle Size ; Phagocytosis ; drug effects ; Polyethylene Glycols ; chemistry ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins ; pharmacokinetics ; Tumor Necrosis Factor-alpha ; administration & dosage ; pharmacokinetics
7.Segmenting lung fields in serial chest radiographs using both population and patient-specific shape statistics.
Yong-hong SHI ; Fei-hu QI ; Hong-xia LUAN ; Guo-rong WU
Chinese Journal of Medical Instrumentation 2006;30(4):264-255
This paper presents a new deformable model using both population-based and patient-specific shape statistics to segment lung fields from serial chest radiographs. First, a modified scale-invariant feature transform (SIFT) local descriptor is used to characterize the image features in the vicinity of each pixel, so that the deformable model deforms in a way that seeks for the region with similar SIFT local descriptors; second, the deformable model is constrained by both population-based and patient-specific shape statistics. At first, population-based shape statistics plays an leading role when the number of serial images is small, and gradually, patient-specific shape statistics plays a more and more important role after a sufficient number of segmentation results on the same patient have been obtained. The proposed deformable model can adapt to the shape variability of different patients, and obtain more robust and accurate segmentation results.
Algorithms
;
Artificial Intelligence
;
Computer Simulation
;
Data Interpretation, Statistical
;
Humans
;
Lung
;
diagnostic imaging
;
Lung Diseases
;
diagnosis
;
Models, Statistical
;
Pattern Recognition, Automated
;
methods
;
Radiographic Image Enhancement
;
methods
;
Radiographic Image Interpretation, Computer-Assisted
;
methods
;
Radiography, Thoracic
;
methods
;
Reproducibility of Results
;
Sensitivity and Specificity
8.Research progress of DNA hydrogel
Jiao-Jiao CHEN ; Shi-Rui YUAN ; Ya SONG ; Qiao-Fang MA ; Xiao-Le QI ; Zheng-Hong WU
Journal of International Pharmaceutical Research 2017;44(10):935-940,946
DNA hydrogels,combining the features of both DNA and hydrogels macromolecules,are endowed with the biologi?cal characters of DNA and the framed structure of hydrogels skeleton.Currently,most DNA hydrogels can achieve sensitive response to temperature,pH,light,and small molecule stimuli,by introducing specific groups or sequences into their backbone.Therefore, the functional properties of DNA hydrogels can be further improved.In this review,we introduce the mentioned stimuli-response DNA hydrogels,as well as their applications in drug controlled-releasing,targeted cancer therapy,biosensor and others.Finally,the pros?pects in the development of DNA hydrogels are also mentioned.
9.Quantitative analysis of circulating DNA in serum of cancer patients.
Hong TU ; Hai-feng GAO ; Shi-long FU ; Hao CHEN
Chinese Journal of Oncology 2004;26(10):606-608
OBJECTIVETo develop a method for ng quantitation of circulating DNA in serum and explore the value in the diagnosis of cancer.
METHODSSerum DNA was extracted by commercial "genomic DNA extraction kit" and detected by fluorescent dye (SYBR green I) staining. Loss of heterozygosity (LOH) at BRCA1 (D17S579, D17S855) and p53 (TP53, D17S786) in serum DNA was analyzed by PCR-based method.
RESULTSSYBR green I dot staining could detect DNA as low as 2 ng. Using this method, we detected serum samples from 483 patients with various types of cancer and 150 healthy individuals. The mean DNA concentration in the normal controls was 22.2 +/- 13.4 ng/ml, while that in cancer patients was 81.3 +/- 98.3 ng/ml (P < 0.001). In 33 ovarian cancer patients with increased DNA level, 27(81.8%) displayed LOH in at least one of the four loci analyzed.
CONCLUSIONCirculating DNA in serum may become additional tumor marker for the diagnosis of cancer.
BRCA1 Protein ; genetics ; Biomarkers, Tumor ; blood ; DNA, Neoplasm ; blood ; genetics ; Female ; Genes, BRCA1 ; Genes, p53 ; Humans ; Loss of Heterozygosity ; Male ; Microsatellite Repeats ; Neoplasms ; blood ; genetics ; Tumor Suppressor Protein p53 ; genetics
10.Series orthodontic treatment on teeth transposition of maxillary canine and lateral incisor.
Shi-tong JIANG ; Bao-lan WANG ; Hong-jie LIU ; Guang-jun JIAO ; Zhong-jun AN ; Liang-kun JIANG
West China Journal of Stomatology 2010;28(1):74-80
OBJECTIVETo explore the treatment on teeth transposition of maxillary canine and lateral incisor in order to improve the clinical treatment effect.
METHODSEleven patients with transposition maxillary canine and lateral incisor were treated with the method: Expand space, artificial reverse occlusion of transpositional lateral incisor to give way, transpositional lateral canine distalization and controlling root, mesial movement of lateral incisor with tongue arch, interactive controlling roots and retention with tongue fixed retaining appliance.
RESULTSEleven patients had satisfactory treatment effect, with tidy dentition and parallel teeth roots of transpositional canine and lateral incisor.
CONCLUSIONSeries orthodontic treatment on teeth transposition of maxillary canine and lateral incisor can effectively improve the clinical treatment effect and shorten the treatment time.
Cuspid ; Humans ; Incisor ; Malocclusion