Objective To understand the acceptability and relevant impact factors of provider initiated testing counseling (abbreviated as PITC) in Liangshan Yi autonomous prefecture,so as to promote strategies for the sustainable development of PITC in AIDS epidemic areas.Methods Four medical institutions were selected with rates of acceptance counted.Multi-factors logistic regression was used to analyze the impact factors from the completed questionnaires.Results Among the 413respondents,197 patients accepted the PITC,with an acceptance rate as 47.7％ (95％ CI:42.9％-52.3％).Factors as being female (OR=7.283,95％CI:3.933-13.465),acceptance of publicity (OR=1.855,95％ CI:1.013-3.395),worried about being infected of HIV/AIDS (OR=2.699,95％ CI:1.616-4.506) etc.were protect factors of PITC,while those who previously received HIV testing (OR=0.226,95％CI:0.124-0.409),afraid of taking blood test (OR=0.052,95％CI:0.024-0.106),knowing more AIDS related knowledge (OR=0.446,95％ CI:0.258-0.773) would significantly reduce the rate of acceptance.Conclusion Nearly half of the outpatients from medical institutions were willing to accept PITC in Liangshan Yi prefecture,thus provided important chance for HIV screening.Ways as strengthening related counseling before and after HIV test,launching effective HIV/AIDS-related publicity and advocating correct knowledge on blood testing,etc.need to be promoted,in order to improve the acceptability of PITC.