Objective To observe the biological characteristics and analyse primary therapeutic response of acute erythroid leukemia.Methods The data of 28 patients primarily diagnosed as acute erythroid leukemia were analyzed.The patients were divided into with muhilineage dysplasia group and without muhilineage dysplasia group,and the morphology,immunology,cytogenetics and molecular biology characteristics and the complete remission rate of the first induction therapy were compared.Results There were 14 cases(50％)with muhilineage dysplasia,which involved in two lineage or trilineage.In 6 cases by flow cytometry,the myeloid blast immunophenotypes were common expressed.In 8 cases detected by karyotype analysis,5 cases were chromosomal abnormal,including 4 cases were complex chromosomal abnormal,1 case was trisomy 8.In 4 cases underwent WT1 detection,all of them were positive.The complete remission rate of the first induction therapy was 39.29％(11/28),the ratein the multilineage dysplasia group was 35.71％(5/14),and the ratein without multilineage dysplasia group was 42.86％(6/14),the difference had no statistical significance(P＞0.05).The complete remission rate of the complex chromosome group was 25.00％(1/4),the intermediate prognostic group was 50.00％(2/4).Conclusions Acute erythroid leukemia had special biological features different from other subtype AML:accompanyed with high frequency of multilineage dysplasia.The abnormality of karyotype were high,and it was often complex karyotype involved with chromosome 5 and/or chromosome 7,which had a low complete remission rate.The complete remission rate of chemotherapy was low,treatment effect was poor.

The prevalence of prediabetes is increasing rapidly in China, and with higher prevalence in coronary heart disease and hypertension patients. Diabetes can be controlled and prevented, previous studies have confirmed that lifestyle interventions and drug interventions can delay the progression of prediabetes to diabetes, and acarbose can be used for the primary prevention of cardiovascular events in impaired glucose tolerance(IGT) and diabetic patients, but benefits of drug intervention for secondary prevention of cardiovascular disease in IGT population are uncertain. The Acarbose Cardiovascular Evaluation(ACE) Study is a multicentre, randomized, placebo-controlled, double-blind study, with the aim of assessing the use of acarbose to reduce the risk of cardiovascular events and the onset of diabetes in IGT patients with cardiovascular disease in China , the results will be very meaningful and valuable for the prevention and treatment of diabetes. We will predict the results of ACE Study based on previous evidences.

Hydroxyapatite has already been considered as an excellent medical biomaterial.and nanometer hydroxyapatite possesses special characteristics.Hydroxyapatite nanoparticles can inhibit the growth of a wide range of cancer cells.but have no effect on normal cells.thus it has a better selectivity in the treatment of cancer.The anti-tumor mechanism of nano-hydroxyapatite is relevant on the following factors:role in the cell membrane,affecting DNA synthesis,inducing cell cycle blockage and apoptosis,impacting the activity of telomerase.This study is designed to introduce the specific structure and characterjstics of nano-hydroxyapatite,analyze the research progress of nano-hydroxyapatite in tumor field.and discuss the possibility of nano-hydroxyapatite as a new anti-cancer drug in clinical application.

Objective To observe the growth inhibition effect of sustained-release fluorouracil implantation in nude mice with human colorectal carcinoma and to discuss its influence on caspase-3 expression .Methods The colorectal carcinoma subcutaneous transplanted model with LoVo cell in BALB/c nude mice was established and divided into 4 groups randomly :the tail intravenous injection of PBS (A group) ,the tail intravenous injection of 5-FU group (B group) ,the intratumor injection of 5-FU group (C group);and the implant of sustained-release fluorouracil group(D group) .After 15 d of medication ,the tumor weight and tumor volume were recorded ,the tumor inhibiting rates were calculated .The tumor tissues were observed by HE staining and detected the expression of caspase-3 by immunohistochemistry (IHC) .Results Sustained-release fluorouracil could suppress the transplantation tumor growth significantly ,the tumor weight and tumor volume in the D group had statistical differences compared with the A ,B and C groups(P<0 .05) ,which could up-regulated the expression of caspase-3 ,the differences between the D group with the A ,B and C groups (P<0 .05) .Conclusion Sustained-release fluorouracil can inhibit the grow th of colorectal carcinoma transplantation tumor more effectively than conventional fluorouracil and up-regulate the expression of caspase-3 ,which prompts that sustained-re-lease fluorouracil may play the anti-tumor role by inducing the tumor cell apoptosis via apoptosis pathway .Its definite tumor-inhibi-ting effect deserves to be promoted in clinical chemotherapy .

Objective To study the correlation between chronic gastritis with benign mucosal nodular-change,Helicobacter pylori(H.pylori)infection and lymphoid follicles infiltration.Methods During July 1,2004 to June 30,2005 patients with chronic gastritis and benign mucosal nodular-change were identified by chromo-endoscopy with anabrosis indigo carmine staining at the antrum.Multiple biopsies were obtained for H.pylori detection with quick test and for pathology examination of mucosal lymphoid follicles formation and lymphocyte infiltration,as well as H.pylori infection.Results The patients were divided into nodular gastritis group,atrophy gastritis group and verrucous gastritis group with mean age of(31.00?11.62),(58.61?12.14)and(51.29?12.99)years old,respectively.The patients with nodular gastritis were the youngest(P

Objective To identify whether soybean isoflavones plays a role in the prevention of chronicallograft nephropathy(CAN)in rats.Methods Forty-five male Lewis rats as receptors were randomly divided into 3 groups,receiving a diet with protein from high isoflavone soy protein fraction (HIS),low isoflavone soy protein fraction(LIS)or casein(CAS)starting one week before renal transplants from fisher donors,and continuing throughout the study.Serial serum and urine samples were collected at the beginning of the study,and at 4th,12th and 24th week.All rats were killed and the grafts were harvested in the 24th week.The functional and histopathogical changes of renal grafts were evaluated.Results In HIS group,arteria caudilis systolic blood pressure(SBP)value,24 h uri- nary protein(Upro)and serum creatitine(Cr)concentrations were significantly lower than those in LIS and CAS groups(all P

Objective To compare the short-term efficacy between da Vinci robotic and laparoscopic radical gastrectomy for gastric cancer.Methods Database including PubMed,EMBASE,the Cochrane Library,Medline,the China National Knowledge Infrastructure (CNKI),Wanfang Database,VIP and China Biomedicine were searched with the key words of gastric cancer,gastrectomy,da Vinci surgical system,laparoscopic,laparoscopy,胃癌,胃切除术,达芬奇机器人手术系统and腹腔镜.Literatures published between May 2002 and March 2014 were retrieved.Related controlled trials containing the clinical efficacy of da Vinci robotic and laparoscopic radical gastrectomy for gastric cancer were enrolled in the study,and then the literatures were screened and the data were extracted by 2 independent reviewers.The quality of the literatures was assessed,and the data were analyzed using the RevMan 5.2 software.Patients receiving da Vinci robotic surgery and laparoscopic surgery were allocated into the robotic group and laparoscopy group,respectively.The count data were analyzed using the relative risk (RR) and 95％ confidence interval (95％ CI),and the measurement data were analyzed using weighted mean difference (WMD) and 95％ CI.The heterogeneity of the data was analyzed using the I2 test.Results Eleven literatures including 3 698 cases were enrolled in the study.There were 953 patients in the robotic group and 2 745 patients in the laparoscopic group.There were significant differences in the operation time,volume of intraoperative blood loss,time of postoperative flatus,time of first oral intake and duration of postoperative hospital stay between the 2 groups (WMD =51.23,-36.96,-0.24,-0.26,-1.11,95％ CI:28.01-74.46,-61.28--12.65,-0.42--0.06,-0.39--0.14,-1.92--0.29,P ＜ 0.05).There was no significant difference in the number of lymph nodes resected,the distance to proximal and distal resection margin and overall incidence of postoperative complications (WMD =1.63,0.21,0.06,RR =1.11,95％ CI:-0.49-3.76,-0.65-1.06,-0.13-0.25,0.90-1.37,P ＞ 0.05).Conclusion Da Vinci robotic radical gastrectomy for gastric cancer is safe and feasible,with the advantages of minimal invasion and rapid postoperative recovery.

Background Cytokines play a crucial role in mediating immune tolerance or immune rejection of corneal transplantation.However,the study on the expression of cytokines in corneal graft is seldom.ObjectiveThe purpose of the study is to investigate the change of cytokine expression in allografts at different time points after corneal transplantation.Methods BALB/c and C57BL/6 mice aged 6 to 8-week old were used to establish autologous and allografts keratoplasty models.BALB/c mouse was use as donor and receipt in autologous group,and the cornea of C57BL/6 mouse was used to graft on the BALB/c mouse in the allografts group.The graft inflammation was clinically scored,and graft inflammatory scores of ≥5 or opaciflcation scores of ≥2 were identified as rejection.BALB/c mice were randomized into normal control group(3 mice)and allografts group(15 mice).Reverse transcriptPCR (RT-PCR) was used to detect the expression of interleukin-4 (IL-4) mRNA,interferon-γ (IFN-γ) mRNA,IL-10mRNA and tumor necrosis factor(TNF-α) mRNA in graft 6 hours and 1 day,3,7,14 days after operation.Results The corneal graft opacification score was ＜2 and inflammatory score was ＜5 in the 10 mice with autologous keratoplasty until 60 days with the survival rate 100％.The edema,opacification and new blood vessel were seen in the BALB/c mice received allografts keratoplasty.The inflammatory score was ≥ 5 and the opacification score was ≥2 24 days after surgery with the rejection rate 100％,in the allografts group,and the graft survival time was (17.80±4.66)days.RT-PCR showed that IL-4 and IFN-γ were positively expressed,and IL-10 and TNF-α were absently expressed in normal mouse cornea.In the allografts keratoplasty mice,positive responses for IL-4,IFN-γ and IL-10 were found in 6hours after operation,but TNF-α was absent.From 1 day through 3 days after operation,the expressions of IL-4,IFN-γand TNF-α were enhanced but IL-10 was disappeared in the graft.IL-10,IFN-γ and TNF-α were expressed till the 7th day,but on the 14th day,only IL-10 was detected in graft in the allografts keratoplasty mice.Conclusions TNF-αis a main factor among the variety of cytokines that may influent corneal allograft rejection locally.

The pathologic grading and clinical staging systems have some restrictions for the evaluation of the prognosis of prostate cancer.Early detection of prostate cancer with clinically sensitive diagnostic methods and / or tumor specific markers is of prime importance.Recently,close attention has been paid to the effects of genes related to apoptosis and tumor suppressor genes on the development,progression and prognostic value of prostate cancer.The importance of enzymes/proteins and hormone receptors have also received more and more emphasis in the studies of prostate cancer.

AIM: To establish a normal HPLC fingerprint chromatogram of herba Taraxaci in Henan Province. METHODS: 40 different kinds of herba Taraxaci were determined by RP-HPLC.Methanolthe solution of NaH_2PO_4(0.02 mol/L)(pH=3.8) gradient elution were adopted as a mobile phase,80 min the recording chromatogram chart and 1.0 mL/min of the velocity of flow detection wavelength was at 323 nm,column temperature was at 35℃. RESULTS: 9 steady mutual peaks were indicated. CONCLUSION: This method is simple,credible and of good reproducibility,can be used to identify and evaluate herba Taraxaci.